Restriction map for
pFN21AE0904
(Linear) (Six-base) MAPSORT of: KIEE0904.seq Check: 1791 from: 1 to: 1071
KIEE0904.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AccI GT'mk_AC
Cuts at: 0 214 1071
Size: 214 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 320 337 854 1071
Size: 284 36 17 517 217
Fragments arranged by size:
517 284 217 36 17
AfeI AGC'GCT
Cuts at: 0 32 616 1071
Size: 32 584 455
Fragments arranged by size:
584 455 32
AflIII A'CryG_T
Cuts at: 0 962 1071
Size: 962 109
AleI CACnn'nnGTG
Cuts at: 0 90 1071
Size: 90 981
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 753 785 1071
Size: 753 32 286
Fragments arranged by size:
753 286 32
AvaI C'yCGr_G
Cuts at: 0 34 201 1071
Size: 34 167 870
Fragments arranged by size:
870 167 34
BanI G'GyrC_C
Cuts at: 0 179 1071
Size: 179 892
BanII G_rGCy'C
Cuts at: 0 528 638 805 1071
Size: 528 110 167 266
Fragments arranged by size:
528 266 167 110
BbsI GAAGACnn'nnnn_
Cuts at: 0 106 581 1071
Size: 106 475 490
Fragments arranged by size:
490 475 106
BglI GCCn_nnn'nGGC
Cuts at: 0 1031 1071
Size: 1031 40
BlpI GC'TnA_GC
Cuts at: 0 637 1071
Size: 637 434
BmgBI CAC'GTC
Cuts at: 0 950 965 1071
Size: 950 15 106
Fragments arranged by size:
950 106 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 1071
Size: 146 925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 1071
Size: 1026 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1071
Size: 307 764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1071
Size: 674 397
BsaAI yAC'GTr
Cuts at: 0 922 1071
Size: 922 149
BsaHI Gr'CG_yC
Cuts at: 0 560 1023 1071
Size: 560 463 48
Fragments arranged by size:
560 463 48
BsaWI w'CCGG_w
Cuts at: 0 753 1071
Size: 753 318
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 691 721 1071
Size: 691 30 350
Fragments arranged by size:
691 350 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 564 688 896 1071
Size: 418 146 124 208 175
Fragments arranged by size:
418 208 175 146 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 238 1071
Size: 116 122 833
Fragments arranged by size:
833 122 116
BsiEI CG_ry'CG
Cuts at: 0 631 914 1071
Size: 631 283 157
Fragments arranged by size:
631 283 157
BsiHKAI G_wGCw'C
Cuts at: 0 68 346 1071
Size: 68 278 725
Fragments arranged by size:
725 278 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 240 1071
Size: 240 831
Bsp1286I G_dGCh'C
Cuts at: 0 68 346 528 638 805 1071
Size: 68 278 182 110 167 266
Fragments arranged by size:
278 266 182 167 110 68
BstYI r'GATC_y
Cuts at: 0 267 1071
Size: 267 804
BstZ17I GTA'TAC
Cuts at: 0 215 1071
Size: 215 856
BtgI C'CryG_G
Cuts at: 0 366 926 975 1071
Size: 366 560 49 96
Fragments arranged by size:
560 366 96 49
BtsI GCAGTG_nn'
Cuts at: 0 66 1035 1071
Size: 66 969 36
Fragments arranged by size:
969 66 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 804 839 1071
Size: 804 35 232
Fragments arranged by size:
804 232 35
EaeI y'GGCC_r
Cuts at: 0 665 911 1032 1071
Size: 665 246 121 39
Fragments arranged by size:
665 246 121 39
EagI C'GGCC_G
Cuts at: 0 911 1071
Size: 911 160
EarI CTCTTCn'nnn_
Cuts at: 0 169 238 467 479 814 830 995 1071
Size: 169 69 229 12 335 16 165 76
Fragments arranged by size:
335 229 169 165 76 69 16 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 307 320 337 854 1071
Size: 284 23 13 17 517 217
Fragments arranged by size:
517 284 217 23 17 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 1071
Size: 319 752
EcoO109I rG'GnC_Cy
Cuts at: 0 740 1071
Size: 740 331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 325 1071
Size: 325 746
FspI TGC'GCA
Cuts at: 0 852 1071
Size: 852 219
HaeII r_GCGC'y
Cuts at: 0 34 618 1071
Size: 34 584 453
Fragments arranged by size:
584 453 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 620 652 691 723 829 861
Size: 550 32 38 32 39 32 106 32
Cuts at: 861 1071
Size: 210
Fragments arranged by size:
550 210 106 39 38 32 32 32
32
HincII GTy'rAC
Cuts at: 0 968 1071
Size: 968 103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1071
Size: 830 241
MscI TGG'CCA
Cuts at: 0 667 1071
Size: 667 404
MslI CAynn'nnrTG
Cuts at: 0 90 166 725 1071
Size: 90 76 559 346
Fragments arranged by size:
559 346 90 76
MspA1I CmG'CkG
Cuts at: 0 210 230 910 977 1071
Size: 210 20 680 67 94
Fragments arranged by size:
680 210 94 67 20
NotI GC'GGCC_GC
Cuts at: 0 911 1071
Size: 911 160
PfoI T'CCnGG_A
Cuts at: 0 122 416 1071
Size: 122 294 655
Fragments arranged by size:
655 294 122
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 142 174 1071
Size: 142 32 897
Fragments arranged by size:
897 142 32
PstI C_TGCA'G
Cuts at: 0 540 1071
Size: 540 531
PvuII CAG'CTG
Cuts at: 0 210 1071
Size: 210 861
SacII CC_GC'GG
Cuts at: 0 978 1071
Size: 978 93
SapI GCTCTTCn'nnn_
Cuts at: 0 830 995 1071
Size: 830 165 76
Fragments arranged by size:
830 165 76
SfcI C'TryA_G
Cuts at: 0 536 1071
Size: 536 535
SmaI CCC'GGG
Cuts at: 0 203 1071
Size: 203 868
SmlI C'TyrA_G
Cuts at: 0 689 1071
Size: 689 382
StuI AGG'CCT
Cuts at: 0 194 1071
Size: 194 877
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 272 695 729 1071
Size: 272 423 34 342
Fragments arranged by size:
423 342 272 34
Tth111I GACn'n_nGTC
Cuts at: 0 705 1071
Size: 705 366
XmaI C'CCGG_G
Cuts at: 0 201 1071
Size: 201 870
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AloI AvaI BanI
BanII BbsI BglI BlpI BmgBI BmrI BplI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BstYI BstZ17I BtgI BtsI CspCI
EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NotI
PfoI PsrI PstI PvuII SacII SapI SfcI SmaI
SmlI StuI TaqII Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII Bme1580I BmtI Bpu10I BsaI BsaBI BseYI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI
ClaI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI TstI XbaI XcmI XhoI XmnI ZraI