Restriction map for
pFN21AE0899
(Linear) (Six-base) MAPSORT of: KIEE0899.seq Check: 2169 from: 1 to: 3738
KIEE0899.orf
With 182 enzymes: SgfI *
November 8, 2010 13:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 940 3738
Size: 940 2798
AatII G_ACGT'C
Cuts at: 0 584 3403 3738
Size: 584 2819 335
Fragments arranged by size:
2819 584 335
AccI GT'mk_AC
Cuts at: 0 622 3738
Size: 622 3116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 275 779 3553 3738
Size: 130 145 504 2774 185
Fragments arranged by size:
2774 504 185 145 130
AflIII A'CryG_T
Cuts at: 0 651 1449 3738
Size: 651 798 2289
Fragments arranged by size:
2289 798 651
AhdI GACnn_n'nnGTC
Cuts at: 0 2130 3738
Size: 2130 1608
AleI CACnn'nnGTG
Cuts at: 0 2621 2717 2828 3738
Size: 2621 96 111 910
Fragments arranged by size:
2621 910 111 96
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1183 2607 3738
Size: 1183 1424 1131
Fragments arranged by size:
1424 1183 1131
AlwNI CAG_nnn'CTG
Cuts at: 0 1881 2934 3432 3738
Size: 1881 1053 498 306
Fragments arranged by size:
1881 1053 498 306
ApaI G_GGCC'C
Cuts at: 0 1205 1867 3738
Size: 1205 662 1871
Fragments arranged by size:
1871 1205 662
ApaLI G'TGCA_C
Cuts at: 0 1597 2587 2989 3085 3738
Size: 1597 990 402 96 653
Fragments arranged by size:
1597 990 653 402 96
ApoI r'AATT_y
Cuts at: 0 123 3738
Size: 123 3615
AseI AT'TA_AT
Cuts at: 0 1985 3738
Size: 1985 1753
AvaI C'yCGr_G
Cuts at: 0 1177 1614 1789 2786 3033 3738
Size: 1177 437 175 997 247 705
Fragments arranged by size:
1177 997 705 437 247 175
BamHI G'GATC_C
Cuts at: 0 3037 3738
Size: 3037 701
BanI G'GyrC_C
Cuts at: 0 2914 3738
Size: 2914 824
BanII G_rGCy'C
Cuts at: 0 185 1205 1867 1934 1958 2267 2279 3263
Size: 185 1020 662 67 24 309 12 984
Cuts at: 3263 3733 3738
Size: 470 5
Fragments arranged by size:
1020 984 662 470 309 185 67 24
12 5
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 2353 3738
Size: 584 1769 1385
Fragments arranged by size:
1769 1385 584
BbvCI CC'TCA_GC
Cuts at: 0 2861 3738
Size: 2861 877
BciVI GTATCCnnnnn_n'
Cuts at: 0 3261 3738
Size: 3261 477
BfrBI ATG'CAT
Cuts at: 0 253 3738
Size: 253 3485
BglI GCCn_nnn'nGGC
Cuts at: 0 1651 3341 3738
Size: 1651 1690 397
Fragments arranged by size:
1690 1651 397
BglII A'GATC_T
Cuts at: 0 3419 3584 3738
Size: 3419 165 154
Fragments arranged by size:
3419 165 154
BlpI GC'TnA_GC
Cuts at: 0 3158 3738
Size: 3158 580
Bme1580I G_kGCm'C
Cuts at: 0 1205 1601 1867 2591 2993 3089 3686 3738
Size: 1205 396 266 724 402 96 597 52
Fragments arranged by size:
1205 724 597 402 396 266 96 52
BmgBI CAC'GTC
Cuts at: 0 3681 3738
Size: 3681 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 1094 1208 2334 2626 3096 3738
Size: 1094 114 1126 292 470 642
Fragments arranged by size:
1126 1094 642 470 292 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 401 1501 2176 2209 2571 2750 3505
Size: 368 33 1100 675 33 362 179 755
Cuts at: 3505 3690 3738
Size: 185 48
Fragments arranged by size:
1100 755 675 368 362 185 179 48
33 33
Bpu10I CC'TnA_GC
Cuts at: 0 887 1736 2372 2861 3262 3738
Size: 887 849 636 489 401 476
Fragments arranged by size:
887 849 636 489 476 401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 2305 3738
Size: 1204 1101 1433
Fragments arranged by size:
1433 1204 1101
BsaI GGTCTCn'nnnn_
Cuts at: 0 2783 2789 3118 3738
Size: 2783 6 329 620
Fragments arranged by size:
2783 620 329 6
BsaAI yAC'GTr
Cuts at: 0 88 1452 2580 3504 3738
Size: 88 1364 1128 924 234
Fragments arranged by size:
1364 1128 924 234 88
BsaHI Gr'CG_yC
Cuts at: 0 581 1186 2414 3400 3738
Size: 581 605 1228 986 338
Fragments arranged by size:
1228 986 605 581 338
BsaWI w'CCGG_w
Cuts at: 0 1587 1776 3738
Size: 1587 189 1962
Fragments arranged by size:
1962 1587 189
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1543 1573 2101 2131 2953 2983 3738
Size: 1543 30 528 30 822 30 755
Fragments arranged by size:
1543 822 755 528 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 571 1338 2155 2413 2416 2878 2881
Size: 559 12 767 817 258 3 462 3
Cuts at: 2881 3306 3423 3738
Size: 425 117 315
Fragments arranged by size:
817 767 559 462 425 315 258 117
12 3 3
BseYI C'CCAG_C
Cuts at: 0 157 373 1848 1859 2526 3738
Size: 157 216 1475 11 667 1212
Fragments arranged by size:
1475 1212 667 216 157 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1779 1894 3022 3738
Size: 968 811 115 1128 716
Fragments arranged by size:
1128 968 811 716 115
BsiEI CG_ry'CG
Cuts at: 0 883 1427 1495 1511 3738
Size: 883 544 68 16 2227
Fragments arranged by size:
2227 883 544 68 16
BsiHKAI G_wGCw'C
Cuts at: 0 1522 1601 1934 1958 2147 2591 2993 3089
Size: 1522 79 333 24 189 444 402 96
Cuts at: 3089 3263 3495 3733 3738
Size: 174 232 238 5
Fragments arranged by size:
1522 444 402 333 238 232 189 174
96 79 24 5
BsmI GAATG_Cn'
Cuts at: 0 2852 3738
Size: 2852 886
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3391 3738
Size: 3391 347
Bsp1286I G_dGCh'C
Cuts at: 0 185 1205 1522 1601 1867 1934 1958 2147
Size: 185 1020 317 79 266 67 24 189
Cuts at: 2147 2267 2279 2591 2993 3089 3263 3495 3686
Size: 120 12 312 402 96 174 232 191
Cuts at: 3686 3733 3738
Size: 47 5
Fragments arranged by size:
1020 402 317 312 266 232 191 189
185 174 120 96 79 67 47 24
12 5
BspEI T'CCGG_A
Cuts at: 0 1776 3738
Size: 1776 1962
BspHI T'CATG_A
Cuts at: 0 2495 2948 3738
Size: 2495 453 790
Fragments arranged by size:
2495 790 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 677 940 1223 3738
Size: 677 263 283 2515
Fragments arranged by size:
2515 677 283 263
BsrBI CCG'CTC
Cuts at: 0 1710 1938 3738
Size: 1710 228 1800
Fragments arranged by size:
1800 1710 228
BsrDI GCAATG_nn'
Cuts at: 0 152 403 1152 3738
Size: 152 251 749 2586
Fragments arranged by size:
2586 749 251 152
BsrFI r'CCGG_y
Cuts at: 0 1853 2075 2147 2354 3738
Size: 1853 222 72 207 1384
Fragments arranged by size:
1853 1384 222 207 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 3738
Size: 516 3222
BstYI r'GATC_y
Cuts at: 0 1667 2183 3037 3419 3584 3738
Size: 1667 516 854 382 165 154
Fragments arranged by size:
1667 854 516 382 165 154
Bsu36I CC'TnA_GG
Cuts at: 0 1678 3738
Size: 1678 2060
BtgI C'CryG_G
Cuts at: 0 64 824 1019 1505 1658 1922 2283 3170
Size: 64 760 195 486 153 264 361 887
Cuts at: 3170 3446 3738
Size: 276 292
Fragments arranged by size:
887 760 486 361 292 276 264 195
153 64
BtsI GCAGTG_nn'
Cuts at: 0 1013 2589 2591 2628 3738
Size: 1013 1576 2 37 1110
Fragments arranged by size:
1576 1110 1013 37 2
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1209 1244 3738
Size: 1209 35 2494
Fragments arranged by size:
2494 1209 35
DraIII CAC_nnn'GTG
Cuts at: 0 2289 3104 3738
Size: 2289 815 634
Fragments arranged by size:
2289 815 634
DrdI GACnn_nn'nnGTC
Cuts at: 0 2413 3738
Size: 2413 1325
EaeI y'GGCC_r
Cuts at: 0 836 1424 1492 1508 1655 2081 2352 3012
Size: 836 588 68 16 147 426 271 660
Cuts at: 3012 3738
Size: 726
Fragments arranged by size:
836 726 660 588 426 271 147 68
16
EagI C'GGCC_G
Cuts at: 0 1424 1492 1508 3738
Size: 1424 68 16 2230
Fragments arranged by size:
2230 1424 68 16
EarI CTCTTCn'nnn_
Cuts at: 0 127 1328 1510 2371 2390 2558 3738
Size: 127 1201 182 861 19 168 1180
Fragments arranged by size:
1201 1180 861 182 168 127 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1363 1564 1677 3373 3738
Size: 1363 201 113 1696 365
Fragments arranged by size:
1696 1363 365 201 113
EcoICRI GAG'CTC
Cuts at: 0 1932 1956 3261 3731 3738
Size: 1932 24 1305 470 7
Fragments arranged by size:
1932 1305 470 24 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 275 368 401 779 1501 2176 2209
Size: 130 145 93 33 378 722 675 33
Cuts at: 2209 2571 2750 3505 3553 3690 3738
Size: 362 179 755 48 137 48
Fragments arranged by size:
755 722 675 378 362 179 145 137
130 93 48 48 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2655 3738
Size: 2655 1083
EcoO109I rG'GnC_Cy
Cuts at: 0 1287 1405 1419 1682 1863 1864 3655 3738
Size: 1287 118 14 263 181 1 1791 83
Fragments arranged by size:
1791 1287 263 181 118 83 14 1
EcoRI G'AATT_C
Cuts at: 0 123 3738
Size: 123 3615
EcoRV GAT'ATC
Cuts at: 0 431 3738
Size: 431 3307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 487 3738
Size: 487 3251
FspI TGC'GCA
Cuts at: 0 2053 3738
Size: 2053 1685
HaeII r_GCGC'y
Cuts at: 0 1559 1860 3738
Size: 1559 301 1878
Fragments arranged by size:
1878 1559 301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 1052 1084 1543 1575 1758 1790
Size: 238 32 782 32 459 32 183 32
Cuts at: 1790 2116 2148 2657 2689 3399 3431 3555 3587
Size: 326 32 509 32 710 32 124 32
Cuts at: 3587 3738
Size: 151
Fragments arranged by size:
782 710 509 459 326 238 183 151
124 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 623 692 1113 3738
Size: 623 69 421 2625
Fragments arranged by size:
2625 623 421 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3374 3738
Size: 3374 364
MscI TGG'CCA
Cuts at: 0 2083 3014 3738
Size: 2083 931 724
Fragments arranged by size:
2083 931 724
MslI CAynn'nnrTG
Cuts at: 0 612 650 931 1921 2500 2621 2717 2828
Size: 612 38 281 990 579 121 96 111
Cuts at: 2828 3738
Size: 910
Fragments arranged by size:
990 910 612 579 281 121 111 96
38
MspA1I CmG'CkG
Cuts at: 0 814 1108 1278 1296 1878 2208 3294 3738
Size: 814 294 170 18 582 330 1086 444
Fragments arranged by size:
1086 814 582 444 330 294 170 18
NaeI GCC'GGC
Cuts at: 0 1855 2356 3738
Size: 1855 501 1382
Fragments arranged by size:
1855 1382 501
NcoI C'CATG_G
Cuts at: 0 1658 1922 3170 3446 3738
Size: 1658 264 1248 276 292
Fragments arranged by size:
1658 1248 292 276 264
NgoMIV G'CCGG_C
Cuts at: 0 1853 2354 3738
Size: 1853 501 1384
Fragments arranged by size:
1853 1384 501
NsiI A_TGCA'T
Cuts at: 0 255 3738
Size: 255 3483
NspI r_CATG'y
Cuts at: 0 145 655 670 2004 2049 2505 3738
Size: 145 510 15 1334 45 456 1233
Fragments arranged by size:
1334 1233 510 456 145 45 15
PasI CC'CwG_GG
Cuts at: 0 2920 3738
Size: 2920 818
PciI A'CATG_T
Cuts at: 0 651 3738
Size: 651 3087
PflMI CCAn_nnn'nTGG
Cuts at: 0 1848 2289 3104 3738
Size: 1848 441 815 634
Fragments arranged by size:
1848 815 634 441
PfoI T'CCnGG_A
Cuts at: 0 2186 3251 3738
Size: 2186 1065 487
Fragments arranged by size:
2186 1065 487
PmlI CAC'GTG
Cuts at: 0 1452 2580 3504 3738
Size: 1452 1128 924 234
Fragments arranged by size:
1452 1128 924 234
PpuMI rG'GwC_Cy
Cuts at: 0 3655 3738
Size: 3655 83
PspOMI G'GGCC_C
Cuts at: 0 1201 1863 3738
Size: 1201 662 1875
Fragments arranged by size:
1875 1201 662
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 68 100 3738
Size: 68 32 3638
Fragments arranged by size:
3638 68 32
PstI C_TGCA'G
Cuts at: 0 1295 2093 2930 3392 3738
Size: 1295 798 837 462 346
Fragments arranged by size:
1295 837 798 462 346
PvuII CAG'CTG
Cuts at: 0 1278 1296 3294 3738
Size: 1278 18 1998 444
Fragments arranged by size:
1998 1278 444 18
RsrII CG'GwC_CG
Cuts at: 0 1728 3738
Size: 1728 2010
SacI G_AGCT'C
Cuts at: 0 1934 1958 3263 3733 3738
Size: 1934 24 1305 470 5
Fragments arranged by size:
1934 1305 470 24 5
SalI G'TCGA_C
Cuts at: 0 621 3738
Size: 621 3117
SapI GCTCTTCn'nnn_
Cuts at: 0 1510 3738
Size: 1510 2228
ScaI AGT'ACT
Cuts at: 0 2548 3738
Size: 2548 1190
SfcI C'TryA_G
Cuts at: 0 1291 2089 2926 3388 3738
Size: 1291 798 837 462 350
Fragments arranged by size:
1291 837 798 462 350
SgrAI Cr'CCGG_yG
Cuts at: 0 2147 3738
Size: 2147 1591
SmaI CCC'GGG
Cuts at: 0 3035 3738
Size: 3035 703
SmlI C'TyrA_G
Cuts at: 0 1219 2320 2786 3738
Size: 1219 1101 466 952
Fragments arranged by size:
1219 1101 952 466
SnaBI TAC'GTA
Cuts at: 0 88 3738
Size: 88 3650
SphI G_CATG'C
Cuts at: 0 145 2004 3738
Size: 145 1859 1734
Fragments arranged by size:
1859 1734 145
SspI AAT'ATT
Cuts at: 0 2754 3738
Size: 2754 984
StuI AGG'CCT
Cuts at: 0 2098 3738
Size: 2098 1640
StyI C'CwwG_G
Cuts at: 0 186 839 1658 1691 1922 3131 3170 3446
Size: 186 653 819 33 231 1209 39 276
Cuts at: 3446 3738
Size: 292
Fragments arranged by size:
1209 819 653 292 276 231 186 39
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 869 871 934 1716 3115 3738
Size: 869 2 63 782 1399 623
Fragments arranged by size:
1399 869 782 623 63 2
TatI w'GTAC_w
Cuts at: 0 1010 2546 3738
Size: 1010 1536 1192
Fragments arranged by size:
1536 1192 1010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2953 2985 3029 3061 3738
Size: 2953 32 44 32 677
Fragments arranged by size:
2953 677 44 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1326 2347 3738
Size: 1062 264 1021 1391
Fragments arranged by size:
1391 1062 1021 264
XhoI C'TCGA_G
Cuts at: 0 2786 3738
Size: 2786 952
XmaI C'CCGG_G
Cuts at: 0 3033 3738
Size: 3033 705
XmnI GAAnn'nnTTC
Cuts at: 0 115 467 710 2305 3738
Size: 115 352 243 1595 1433
Fragments arranged by size:
1595 1433 352 243 115
ZraI GAC'GTC
Cuts at: 0 582 3401 3738
Size: 582 2819 337
Fragments arranged by size:
2819 582 337
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbsI BbvCI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PsrI PstI PvuII RsrII SacI SalI SapI ScaI
SfcI SgrAI SmaI SmlI SnaBI SphI SspI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AloI AscI AsiSI
AvrII BaeI BbeI BcgI BclI BmtI BplI BsaBI
BsiWI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI DraI FseI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
PacI PmeI PpiI PshAI PsiI PspXI PvuI SacII
SanDI SbfI SexAI SfiI SfoI SgfI SpeI SrfI
SwaI Tth111I XbaI