Restriction map for
pFN21AE0898
(Linear) (Six-base) MAPSORT of: KIEE0898.seq Check: 9781 from: 1 to: 4107
KIEE0898.orf
With 182 enzymes: SgfI *
September 16, 2010 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1771 4107
Size: 1771 2336
Acc65I G'GTAC_C
Cuts at: 0 1812 4107
Size: 1812 2295
AclI AA'CG_TT
Cuts at: 0 2172 4107
Size: 2172 1935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 1073 1271 1571 3110 4107
Size: 536 537 198 300 1539 997
Fragments arranged by size:
1539 997 537 536 300 198
AflIII A'CryG_T
Cuts at: 0 3033 4107
Size: 3033 1074
AlwNI CAG_nnn'CTG
Cuts at: 0 1314 4107
Size: 1314 2793
ApoI r'AATT_y
Cuts at: 0 194 532 565 741 769 895 1017 1095
Size: 194 338 33 176 28 126 122 78
Cuts at: 1095 1575 1965 2159 2221 2258 2356 3150 4107
Size: 480 390 194 62 37 98 794 957
Fragments arranged by size:
957 794 480 390 338 194 194 176
126 122 98 78 62 37 33 28
AseI AT'TA_AT
Cuts at: 0 269 2387 4107
Size: 269 2118 1720
Fragments arranged by size:
2118 1720 269
AvaI C'yCGr_G
Cuts at: 0 1558 2000 4107
Size: 1558 442 2107
Fragments arranged by size:
2107 1558 442
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1666 1699 1796 1829 4107
Size: 1666 33 97 33 2278
Fragments arranged by size:
2278 1666 97 33 33
BanI G'GyrC_C
Cuts at: 0 1812 3265 4107
Size: 1812 1453 842
Fragments arranged by size:
1812 1453 842
BanII G_rGCy'C
Cuts at: 0 173 4107
Size: 173 3934
BbsI GAAGACnn'nnnn_
Cuts at: 0 357 929 2065 3919 4107
Size: 357 572 1136 1854 188
Fragments arranged by size:
1854 1136 572 357 188
BbvCI CC'TCA_GC
Cuts at: 0 3122 4107
Size: 3122 985
BclI T'GATC_A
Cuts at: 0 302 3134 4107
Size: 302 2832 973
Fragments arranged by size:
2832 973 302
BfrBI ATG'CAT
Cuts at: 0 3421 4107
Size: 3421 686
BglI GCCn_nnn'nGGC
Cuts at: 0 103 4107
Size: 103 4004
BglII A'GATC_T
Cuts at: 0 3363 4107
Size: 3363 744
BlpI GC'TnA_GC
Cuts at: 0 1307 4107
Size: 1307 2800
BmrI ACTGGGnnnn_n'
Cuts at: 0 2431 3283 4107
Size: 2431 852 824
Fragments arranged by size:
2431 852 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 147 1420 1976 2468 2744 4107
Size: 14 133 1273 556 492 276 1363
Fragments arranged by size:
1363 1273 556 492 276 133 14
Bpu10I CC'TnA_GC
Cuts at: 0 878 2050 3122 4107
Size: 878 1172 1072 985
Fragments arranged by size:
1172 1072 985 878
BsaAI yAC'GTr
Cuts at: 0 1462 3034 4107
Size: 1462 1572 1073
Fragments arranged by size:
1572 1462 1073
BsaBI GATnn'nnATC
Cuts at: 0 2465 3570 4107
Size: 2465 1105 537
Fragments arranged by size:
2465 1105 537
BsaWI w'CCGG_w
Cuts at: 0 1988 4107
Size: 1988 2119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 566 775 3110 4107
Size: 566 209 2335 997
Fragments arranged by size:
2335 997 566 209
BseYI C'CCAG_C
Cuts at: 0 942 2944 3593 4107
Size: 942 2002 649 514
Fragments arranged by size:
2002 942 649 514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1586 4107
Size: 1586 2521
BsiEI CG_ry'CG
Cuts at: 0 38 4107
Size: 38 4069
BsiHKAI G_wGCw'C
Cuts at: 0 2454 4107
Size: 2454 1653
BsmI GAATG_Cn'
Cuts at: 0 1524 2709 2743 4107
Size: 1524 1185 34 1364
Fragments arranged by size:
1524 1364 1185 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2794 2893 3736 4107
Size: 2794 99 843 371
Fragments arranged by size:
2794 843 371 99
Bsp1286I G_dGCh'C
Cuts at: 0 173 2454 4107
Size: 173 2281 1653
Fragments arranged by size:
2281 1653 173
BspEI T'CCGG_A
Cuts at: 0 1988 4107
Size: 1988 2119
BspHI T'CATG_A
Cuts at: 0 1083 2112 4107
Size: 1083 1029 1995
Fragments arranged by size:
1995 1083 1029
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1771 4107
Size: 1771 2336
BsrDI GCAATG_nn'
Cuts at: 0 604 3147 3152 4107
Size: 604 2543 5 955
Fragments arranged by size:
2543 955 604 5
BsrFI r'CCGG_y
Cuts at: 0 132 157 4107
Size: 132 25 3950
Fragments arranged by size:
3950 132 25
BsrGI T'GTAC_A
Cuts at: 0 2032 2978 4107
Size: 2032 946 1129
Fragments arranged by size:
2032 1129 946
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3367 4107
Size: 3367 740
BstEII G'GTnAC_C
Cuts at: 0 457 4107
Size: 457 3650
BstYI r'GATC_y
Cuts at: 0 1554 1985 2541 3114 3363 3947 4107
Size: 1554 431 556 573 249 584 160
Fragments arranged by size:
1554 584 573 556 431 249 160
BtgI C'CryG_G
Cuts at: 0 38 3386 4107
Size: 38 3348 721
Fragments arranged by size:
3348 721 38
BtsI GCAGTG_nn'
Cuts at: 0 2241 4107
Size: 2241 1866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3324 3359 3689 3724 4107
Size: 3324 35 330 35 383
Fragments arranged by size:
3324 383 330 35 35
DraI TTT'AAA
Cuts at: 0 843 1725 2465 4107
Size: 843 882 740 1642
Fragments arranged by size:
1642 882 843 740
DrdI GACnn_nn'nnGTC
Cuts at: 0 3321 4107
Size: 3321 786
EaeI y'GGCC_r
Cuts at: 0 35 134 3383 4107
Size: 35 99 3249 724
Fragments arranged by size:
3249 724 99 35
EagI C'GGCC_G
Cuts at: 0 35 4107
Size: 35 4072
EarI CTCTTCn'nnn_
Cuts at: 0 533 626 752 1052 2360 2934 4107
Size: 533 93 126 300 1308 574 1173
Fragments arranged by size:
1308 1173 574 533 300 126 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3406 4107
Size: 3406 701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 147 536 1073 1271 1420 1571 1976
Size: 14 133 389 537 198 149 151 405
Cuts at: 1976 2468 2744 3110 4107
Size: 492 276 366 997
Fragments arranged by size:
997 537 492 405 389 366 276 198
151 149 133 14
EcoO109I rG'GnC_Cy
Cuts at: 0 636 3960 4107
Size: 636 3324 147
Fragments arranged by size:
3324 636 147
EcoRI G'AATT_C
Cuts at: 0 741 1965 4107
Size: 741 1224 2142
Fragments arranged by size:
2142 1224 741
EcoRV GAT'ATC
Cuts at: 0 1981 4107
Size: 1981 2126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1855 2089 3382 4107
Size: 1855 234 1293 725
Fragments arranged by size:
1855 1293 725 234
FseI GG_CCGG'CC
Cuts at: 0 136 4107
Size: 136 3971
HaeII r_GCGC'y
Cuts at: 0 2934 4107
Size: 2934 1173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 945 977 1170 1202 1970 1983 2002 2015
Size: 945 32 193 32 768 13 19 13
Cuts at: 2015 2644 2676 3085 3117 4107
Size: 629 32 409 32 990
Fragments arranged by size:
990 945 768 629 409 193 32 32
32 32 19 13 13
HincII GTy'rAC
Cuts at: 0 3515 4107
Size: 3515 592
HindIII A'AGCT_T
Cuts at: 0 4027 4107
Size: 4027 80
HpaI GTT'AAC
Cuts at: 0 3515 4107
Size: 3515 592
KpnI G_GTAC'C
Cuts at: 0 1816 4107
Size: 1816 2291
MfeI C'AATT_G
Cuts at: 0 1739 3341 4107
Size: 1739 1602 766
Fragments arranged by size:
1739 1602 766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 4107
Size: 1099 3008
MscI TGG'CCA
Cuts at: 0 3385 4107
Size: 3385 722
MspA1I CmG'CkG
Cuts at: 0 34 40 142 148 154 1045 3593 3993
Size: 34 6 102 6 6 891 2548 400
Cuts at: 3993 4107
Size: 114
Fragments arranged by size:
2548 891 400 114 102 34 6 6
6
NaeI GCC'GGC
Cuts at: 0 134 159 4107
Size: 134 25 3948
Fragments arranged by size:
3948 134 25
NcoI C'CATG_G
Cuts at: 0 3386 4107
Size: 3386 721
NdeI CA'TA_TG
Cuts at: 0 627 4107
Size: 627 3480
NgoMIV G'CCGG_C
Cuts at: 0 132 157 4107
Size: 132 25 3950
Fragments arranged by size:
3950 132 25
NotI GC'GGCC_GC
Cuts at: 0 35 4107
Size: 35 4072
NsiI A_TGCA'T
Cuts at: 0 3423 4107
Size: 3423 684
NspI r_CATG'y
Cuts at: 0 1302 2458 2506 4107
Size: 1302 1156 48 1601
Fragments arranged by size:
1601 1302 1156 48
PfoI T'CCnGG_A
Cuts at: 0 2760 4107
Size: 2760 1347
PmlI CAC'GTG
Cuts at: 0 1462 4107
Size: 1462 2645
PpuMI rG'GwC_Cy
Cuts at: 0 636 4107
Size: 636 3471
PshAI GACnn'nnGTC
Cuts at: 0 2905 4107
Size: 2905 1202
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2093 2125 3066 3098 4107
Size: 2093 32 941 32 1009
Fragments arranged by size:
2093 1009 941 32 32
PstI C_TGCA'G
Cuts at: 0 1044 2742 3309 4073 4107
Size: 1044 1698 567 764 34
Fragments arranged by size:
1698 1044 764 567 34
PvuII CAG'CTG
Cuts at: 0 1045 3593 3993 4107
Size: 1045 2548 400 114
Fragments arranged by size:
2548 1045 400 114
SacII CC_GC'GG
Cuts at: 0 41 4107
Size: 41 4066
SapI GCTCTTCn'nnn_
Cuts at: 0 533 2934 4107
Size: 533 2401 1173
Fragments arranged by size:
2401 1173 533
ScaI AGT'ACT
Cuts at: 0 1806 4107
Size: 1806 2301
SexAI A'CCwGG_T
Cuts at: 0 2017 4107
Size: 2017 2090
SfcI C'TryA_G
Cuts at: 0 989 1040 2738 3305 4069 4107
Size: 989 51 1698 567 764 38
Fragments arranged by size:
1698 989 764 567 51 38
SmlI C'TyrA_G
Cuts at: 0 2000 4107
Size: 2000 2107
SphI G_CATG'C
Cuts at: 0 1302 2506 4107
Size: 1302 1204 1601
Fragments arranged by size:
1601 1302 1204
SspI AAT'ATT
Cuts at: 0 2347 2595 2793 4107
Size: 2347 248 198 1314
Fragments arranged by size:
2347 1314 248 198
StuI AGG'CCT
Cuts at: 0 561 4107
Size: 561 3546
StyI C'CwwG_G
Cuts at: 0 473 1610 3386 3750 4107
Size: 473 1137 1776 364 357
Fragments arranged by size:
1776 1137 473 364 357
SwaI ATTT'AAAT
Cuts at: 0 2465 4107
Size: 2465 1642
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3785 4107
Size: 3785 322
TatI w'GTAC_w
Cuts at: 0 1804 2032 2325 2483 2978 3654 3765 4107
Size: 1804 228 293 158 495 676 111 342
Fragments arranged by size:
1804 676 495 342 293 228 158 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2941 4107
Size: 2941 1166
XhoI C'TCGA_G
Cuts at: 0 2000 4107
Size: 2000 2107
XmnI GAAnn'nnTTC
Cuts at: 0 342 1202 1965 3926 4107
Size: 342 860 763 1961 181
Fragments arranged by size:
1961 860 763 342 181
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AlwNI ApoI AseI
AvaI BaeI BanI BanII BbsI BbvCI BclI BfrBI
BglI BglII BlpI BmrI BpmI Bpu10I BsaAI BsaBI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstEII BstYI BtgI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FseI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV NotI
NsiI NspI PfoI PmlI PpuMI PshAI PsrI PstI
PvuII SacII SapI ScaI SexAI SfcI SmlI SphI
SspI StuI StyI SwaI TaqII TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI
BcgI BciVI Bme1580I BmgBI BmtI BplI BpuEI BsaI
BsaHI BsaXI BsiWI BsrBI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI DraIII EcoICRI EcoNI FspI
FspAI KasI MluI MslI NarI NheI NruI PacI
PasI PciI PflMI PmeI PpiI PsiI PspOMI PspXI
PvuI RsrII SacI SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI TstI Tth111I
XbaI XmaI ZraI