Restriction map for
pFN21AE0897
(Linear) (Six-base) MAPSORT of: KIEE0897.seq Check: 9033 from: 1 to: 765
KIEE0897.orf
With 182 enzymes: SgfI *
September 9, 2010 13:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 765
Size: 568 197
AflIII A'CryG_T
Cuts at: 0 554 765
Size: 554 211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 765
Size: 559 206
AlwNI CAG_nnn'CTG
Cuts at: 0 366 765
Size: 366 399
ApoI r'AATT_y
Cuts at: 0 229 765
Size: 229 536
BanI G'GyrC_C
Cuts at: 0 695 765
Size: 695 70
BanII G_rGCy'C
Cuts at: 0 92 440 765
Size: 92 348 325
Fragments arranged by size:
348 325 92
BbeI G_GCGC'C
Cuts at: 0 699 765
Size: 699 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 543 765
Size: 543 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 414 424 601 765
Size: 79 335 10 177 164
Fragments arranged by size:
335 177 164 79 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 531 765
Size: 531 234
BsaHI Gr'CG_yC
Cuts at: 0 423 696 765
Size: 423 273 69
Fragments arranged by size:
423 273 69
BsaWI w'CCGG_w
Cuts at: 0 161 765
Size: 161 604
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 481 765
Size: 82 399 284
Fragments arranged by size:
399 284 82
BseYI C'CCAG_C
Cuts at: 0 369 560 765
Size: 369 191 205
Fragments arranged by size:
369 205 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 765
Size: 352 413
BsiHKAI G_wGCw'C
Cuts at: 0 92 400 440 631 746 765
Size: 92 308 40 191 115 19
Fragments arranged by size:
308 191 115 92 40 19
Bsp1286I G_dGCh'C
Cuts at: 0 92 400 440 631 746 765
Size: 92 308 40 191 115 19
Fragments arranged by size:
308 191 115 92 40 19
BspEI T'CCGG_A
Cuts at: 0 161 765
Size: 161 604
BstYI r'GATC_y
Cuts at: 0 177 765
Size: 177 588
BtgI C'CryG_G
Cuts at: 0 5 765
Size: 5 760
BtsI GCAGTG_nn'
Cuts at: 0 56 364 765
Size: 56 308 401
Fragments arranged by size:
401 308 56
EarI CTCTTCn'nnn_
Cuts at: 0 757 765
Size: 757 8
EcoICRI GAG'CTC
Cuts at: 0 90 438 765
Size: 90 348 327
Fragments arranged by size:
348 327 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 414 424 568 601 765
Size: 79 335 10 144 33 164
Fragments arranged by size:
335 164 144 79 33 10
EcoO109I rG'GnC_Cy
Cuts at: 0 12 99 186 765
Size: 12 87 87 579
Fragments arranged by size:
579 87 87 12
HaeII r_GCGC'y
Cuts at: 0 699 765
Size: 699 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 675 707 765
Size: 301 32 342 32 58
Fragments arranged by size:
342 301 58 32 32
HincII GTy'rAC
Cuts at: 0 54 320 765
Size: 54 266 445
Fragments arranged by size:
445 266 54
KasI G'GCGC_C
Cuts at: 0 695 765
Size: 695 70
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 581 765
Size: 497 84 184
Fragments arranged by size:
497 184 84
MslI CAynn'nnrTG
Cuts at: 0 541 765
Size: 541 224
NarI GG'CG_CC
Cuts at: 0 696 765
Size: 696 69
NcoI C'CATG_G
Cuts at: 0 5 765
Size: 5 760
NdeI CA'TA_TG
Cuts at: 0 680 765
Size: 680 85
NspI r_CATG'y
Cuts at: 0 558 765
Size: 558 207
PasI CC'CwG_GG
Cuts at: 0 63 765
Size: 63 702
PciI A'CATG_T
Cuts at: 0 554 765
Size: 554 211
PfoI T'CCnGG_A
Cuts at: 0 95 765
Size: 95 670
PpuMI rG'GwC_Cy
Cuts at: 0 12 99 186 765
Size: 12 87 87 579
Fragments arranged by size:
579 87 87 12
SacI G_AGCT'C
Cuts at: 0 92 440 765
Size: 92 348 325
Fragments arranged by size:
348 325 92
ScaI AGT'ACT
Cuts at: 0 282 765
Size: 282 483
SfoI GGC'GCC
Cuts at: 0 697 765
Size: 697 68
StyI C'CwwG_G
Cuts at: 0 5 765
Size: 5 760
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 446 765
Size: 446 319
TatI w'GTAC_w
Cuts at: 0 280 765
Size: 280 485
XbaI T'CTAG_A
Cuts at: 0 180 765
Size: 180 585
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI BanI BanII BbeI
BplI BpmI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BstYI BtgI BtsI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MslI
NarI NcoI NdeI NspI PasI PciI PfoI PpuMI
SacI ScaI SfoI StyI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI