Restriction map for
pFN21AE0842
(Linear) (Six-base) MAPSORT of: KIEE0842.seq Check: 8797 from: 1 to: 1167
KIEE0842.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 235 910 983 1167
Size: 235 675 73 184
Fragments arranged by size:
675 235 184 73
AccI GT'mk_AC
Cuts at: 0 263 1167
Size: 263 904
AfeI AGC'GCT
Cuts at: 0 442 1057 1167
Size: 442 615 110
Fragments arranged by size:
615 442 110
AleI CACnn'nnGTG
Cuts at: 0 217 1167
Size: 217 950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 538 808 1167
Size: 76 462 270 359
Fragments arranged by size:
462 359 270 76
AlwNI CAG_nnn'CTG
Cuts at: 0 104 1167
Size: 104 1063
ApaI G_GGCC'C
Cuts at: 0 613 668 747 997 1022 1161 1167
Size: 613 55 79 250 25 139 6
Fragments arranged by size:
613 250 139 79 55 25 6
AvaI C'yCGr_G
Cuts at: 0 13 21 667 996 1144 1167
Size: 13 8 646 329 148 23
Fragments arranged by size:
646 329 148 23 13 8
BanI G'GyrC_C
Cuts at: 0 154 514 864 1167
Size: 154 360 350 303
Fragments arranged by size:
360 350 303 154
BanII G_rGCy'C
Cuts at: 0 125 613 668 747 997 1022 1161 1167
Size: 125 488 55 79 250 25 139 6
Fragments arranged by size:
488 250 139 125 79 55 25 6
BbeI G_GCGC'C
Cuts at: 0 868 1167
Size: 868 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 1070 1167
Size: 1070 97
BglI GCCn_nnn'nGGC
Cuts at: 0 488 822 862 1123 1167
Size: 488 334 40 261 44
Fragments arranged by size:
488 334 261 44 40
BlpI GC'TnA_GC
Cuts at: 0 304 1167
Size: 304 863
Bme1580I G_kGCm'C
Cuts at: 0 157 517 613 668 747 997 1022 1161
Size: 157 360 96 55 79 250 25 139
Cuts at: 1161 1167
Size: 6
Fragments arranged by size:
360 250 157 139 96 79 55 25
6
BmgBI CAC'GTC
Cuts at: 0 360 1167
Size: 360 807
BmrI ACTGGGnnnn_n'
Cuts at: 0 837 1167
Size: 837 330
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 144 1167
Size: 144 1023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1167
Size: 154 1013
BsaAI yAC'GTr
Cuts at: 0 267 1167
Size: 267 900
BsaHI Gr'CG_yC
Cuts at: 0 865 1167
Size: 865 302
BseYI C'CCAG_C
Cuts at: 0 124 510 780 1119 1167
Size: 124 386 270 339 48
Fragments arranged by size:
386 339 270 124 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1167
Size: 496 671
BsiEI CG_ry'CG
Cuts at: 0 119 463 754 984 1167
Size: 119 344 291 230 183
Fragments arranged by size:
344 291 230 183 119
BsiHKAI G_wGCw'C
Cuts at: 0 377 1167
Size: 377 790
Bsp1286I G_dGCh'C
Cuts at: 0 125 157 377 517 613 668 747 997
Size: 125 32 220 140 96 55 79 250
Cuts at: 997 1022 1161 1167
Size: 25 139 6
Fragments arranged by size:
250 220 140 139 125 96 79 55
32 25 6
BspHI T'CATG_A
Cuts at: 0 428 1167
Size: 428 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 235 299 308 910 983 1146 1167
Size: 235 64 9 602 73 163 21
Fragments arranged by size:
602 235 163 73 64 21 9
BsrBI CCG'CTC
Cuts at: 0 233 719 1070 1167
Size: 233 486 351 97
Fragments arranged by size:
486 351 233 97
BssHII G'CGCG_C
Cuts at: 0 615 694 726 760 762 764 877 1167
Size: 615 79 32 34 2 2 113 290
Fragments arranged by size:
615 290 113 79 34 32 2 2
BssSI C'ACGA_G
Cuts at: 0 1108 1167
Size: 1108 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 923 1167
Size: 923 244
BstEII G'GTnAC_C
Cuts at: 0 222 1167
Size: 222 945
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 186 567 1167
Size: 132 54 381 600
Fragments arranged by size:
600 381 132 54
BtgI C'CryG_G
Cuts at: 0 149 179 868 1167
Size: 149 30 689 299
Fragments arranged by size:
689 299 149 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 642 1167
Size: 642 525
DrdI GACnn_nn'nnGTC
Cuts at: 0 15 171 1167
Size: 15 156 996
Fragments arranged by size:
996 156 15
EaeI y'GGCC_r
Cuts at: 0 41 146 176 284 323 460 557 751
Size: 41 105 30 108 39 137 97 194
Cuts at: 751 981 1167
Size: 230 186
Fragments arranged by size:
230 194 186 137 108 105 97 41
39 30
EagI C'GGCC_G
Cuts at: 0 460 751 981 1167
Size: 460 291 230 186
Fragments arranged by size:
460 291 230 186
EarI CTCTTCn'nnn_
Cuts at: 0 382 649 802 1167
Size: 382 267 153 365
Fragments arranged by size:
382 365 267 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1167
Size: 154 1013
EcoO109I rG'GnC_Cy
Cuts at: 0 138 580 609 664 855 993 1018 1150
Size: 138 442 29 55 191 138 25 132
Cuts at: 1150 1157 1158 1167
Size: 7 1 9
Fragments arranged by size:
442 191 138 138 132 55 29 25
9 7 1
HaeII r_GCGC'y
Cuts at: 0 7 283 444 483 511 532 868 1059
Size: 7 276 161 39 28 21 336 191
Cuts at: 1059 1167
Size: 108
Fragments arranged by size:
336 276 191 161 108 39 28 21
7
HincII GTy'rAC
Cuts at: 0 273 888 1167
Size: 273 615 279
Fragments arranged by size:
615 279 273
KasI G'GCGC_C
Cuts at: 0 864 1167
Size: 864 303
MscI TGG'CCA
Cuts at: 0 148 325 559 1167
Size: 148 177 234 608
Fragments arranged by size:
608 234 177 148
MslI CAynn'nnrTG
Cuts at: 0 184 217 1167
Size: 184 33 950
Fragments arranged by size:
950 184 33
MspA1I CmG'CkG
Cuts at: 0 465 834 861 870 923 1004 1123 1167
Size: 465 369 27 9 53 81 119 44
Fragments arranged by size:
465 369 119 81 53 44 27 9
NarI GG'CG_CC
Cuts at: 0 865 1167
Size: 865 302
NcoI C'CATG_G
Cuts at: 0 179 1167
Size: 179 988
NotI GC'GGCC_GC
Cuts at: 0 460 981 1167
Size: 460 521 186
Fragments arranged by size:
521 460 186
PasI CC'CwG_GG
Cuts at: 0 34 1154 1167
Size: 34 1120 13
Fragments arranged by size:
1120 34 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 344 1167
Size: 344 823
PpuMI rG'GwC_Cy
Cuts at: 0 138 580 1150 1167
Size: 138 442 570 17
Fragments arranged by size:
570 442 138 17
PspOMI G'GGCC_C
Cuts at: 0 609 664 743 993 1018 1157 1167
Size: 609 55 79 250 25 139 10
Fragments arranged by size:
609 250 139 79 55 25 10
PstI C_TGCA'G
Cuts at: 0 1037 1087 1167
Size: 1037 50 80
Fragments arranged by size:
1037 80 50
PvuII CAG'CTG
Cuts at: 0 834 923 1167
Size: 834 89 244
Fragments arranged by size:
834 244 89
SacII CC_GC'GG
Cuts at: 0 871 1167
Size: 871 296
SanDI GG'GwC_CC
Cuts at: 0 580 1150 1167
Size: 580 570 17
Fragments arranged by size:
580 570 17
SapI GCTCTTCn'nnn_
Cuts at: 0 382 649 802 1167
Size: 382 267 153 365
Fragments arranged by size:
382 365 267 153
SexAI A'CCwGG_T
Cuts at: 0 140 1167
Size: 140 1027
SfcI C'TryA_G
Cuts at: 0 1033 1083 1167
Size: 1033 50 84
Fragments arranged by size:
1033 84 50
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1123 1167
Size: 1123 44
SfoI GGC'GCC
Cuts at: 0 866 1167
Size: 866 301
SmaI CCC'GGG
Cuts at: 0 669 998 1167
Size: 669 329 169
Fragments arranged by size:
669 329 169
SrfI GCCC'GGGC
Cuts at: 0 669 998 1167
Size: 669 329 169
Fragments arranged by size:
669 329 169
StyI C'CwwG_G
Cuts at: 0 179 338 485 1167
Size: 179 159 147 682
Fragments arranged by size:
682 179 159 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 1167
Size: 133 1034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 1167
Size: 186 981
XmaI C'CCGG_G
Cuts at: 0 667 996 1167
Size: 667 329 171
Fragments arranged by size:
667 329 171
Enzymes that do cut:
AarI AccI AfeI AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BsaAI BsaHI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BssHII BssSI BstAPI
BstEII BstXI BtgI BtgZI DrdI EaeI EagI EarI
Eco57MI EcoO109I HaeII HincII KasI MscI MslI MspA1I
NarI NcoI NotI PasI PflMI PpuMI PspOMI PstI
PvuII SacII SanDI SapI SexAI SfcI SfiI SfoI
SmaI SrfI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiWI
BsmI BsmBI BspEI BsrDI BsrFI BsrGI BstBI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SbfI ScaI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI