Restriction map for
pFN21AE0797
(Linear) (Six-base) MAPSORT of: KIEE0797.seq Check: 6890 from: 1 to: 4716
KIEE0797.orf
With 182 enzymes: SgfI *
September 6, 2010 13:28 ..
AccI GT'mk_AC
Cuts at: 0 237 2811 4716
Size: 237 2574 1905
Fragments arranged by size:
2574 1905 237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1544 2869 3326 3346 3388 3887 4716
Size: 1033 511 1325 457 20 42 499 829
Fragments arranged by size:
1325 1033 829 511 499 457 42 20
AflII C'TTAA_G
Cuts at: 0 4027 4716
Size: 4027 689
AflIII A'CryG_T
Cuts at: 0 2906 4716
Size: 2906 1810
AhdI GACnn_n'nnGTC
Cuts at: 0 3618 4716
Size: 3618 1098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 310 2096 2534 4478 4716
Size: 310 1786 438 1944 238
Fragments arranged by size:
1944 1786 438 310 238
AlwNI CAG_nnn'CTG
Cuts at: 0 19 831 1266 2193 3440 3459 4716
Size: 19 812 435 927 1247 19 1257
Fragments arranged by size:
1257 1247 927 812 435 19 19
ApaI G_GGCC'C
Cuts at: 0 322 465 914 2679 4716
Size: 322 143 449 1765 2037
Fragments arranged by size:
2037 1765 449 322 143
ApoI r'AATT_y
Cuts at: 0 1022 2371 2464 3331 3745 4716
Size: 1022 1349 93 867 414 971
Fragments arranged by size:
1349 1022 971 867 414 93
AseI AT'TA_AT
Cuts at: 0 2159 4270 4716
Size: 2159 2111 446
Fragments arranged by size:
2159 2111 446
AvaI C'yCGr_G
Cuts at: 0 578 792 813 907 933 4716
Size: 578 214 21 94 26 3783
Fragments arranged by size:
3783 578 214 94 26 21
AvrII C'CTAG_G
Cuts at: 0 3096 4335 4716
Size: 3096 1239 381
Fragments arranged by size:
3096 1239 381
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3407 3440 4716
Size: 3407 33 1276
Fragments arranged by size:
3407 1276 33
BamHI G'GATC_C
Cuts at: 0 274 1986 2627 2954 3346 4399 4716
Size: 274 1712 641 327 392 1053 317
Fragments arranged by size:
1712 1053 641 392 327 317 274
BanI G'GyrC_C
Cuts at: 0 837 3292 4716
Size: 837 2455 1424
Fragments arranged by size:
2455 1424 837
BanII G_rGCy'C
Cuts at: 0 322 390 465 552 914 1748 2092 2388
Size: 322 68 75 87 362 834 344 296
Cuts at: 2388 2679 3480 4716
Size: 291 801 1236
Fragments arranged by size:
1236 834 801 362 344 322 296 291
87 75 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 266 3614 3995 4215 4421 4716
Size: 266 3348 381 220 206 295
Fragments arranged by size:
3348 381 295 266 220 206
BbvCI CC'TCA_GC
Cuts at: 0 1697 4716
Size: 1697 3019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 560 4716
Size: 526 34 4156
Fragments arranged by size:
4156 526 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1064 4716
Size: 1064 3652
BclI T'GATC_A
Cuts at: 0 1554 2798 2894 4716
Size: 1554 1244 96 1822
Fragments arranged by size:
1822 1554 1244 96
BfrBI ATG'CAT
Cuts at: 0 210 219 4716
Size: 210 9 4497
Fragments arranged by size:
4497 210 9
BglI GCCn_nnn'nGGC
Cuts at: 0 3290 4716
Size: 3290 1426
BglII A'GATC_T
Cuts at: 0 1894 4406 4716
Size: 1894 2512 310
Fragments arranged by size:
2512 1894 310
BlpI GC'TnA_GC
Cuts at: 0 805 2021 2042 3370 4716
Size: 805 1216 21 1328 1346
Fragments arranged by size:
1346 1328 1216 805 21
Bme1580I G_kGCm'C
Cuts at: 0 289 322 465 842 908 914 2679 4452
Size: 289 33 143 377 66 6 1765 1773
Cuts at: 4452 4716
Size: 264
Fragments arranged by size:
1773 1765 377 289 264 143 66 33
6
BmgBI CAC'GTC
Cuts at: 0 399 2052 3939 4716
Size: 399 1653 1887 777
Fragments arranged by size:
1887 1653 777 399
BmrI ACTGGGnnnn_n'
Cuts at: 0 2088 2648 3345 4716
Size: 2088 560 697 1371
Fragments arranged by size:
2088 1371 697 560
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 417 1292 3858 4716
Size: 417 875 2566 858
Fragments arranged by size:
2566 875 858 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 437 940 1237 1315 2170 4366 4414
Size: 387 50 503 297 78 855 2196 48
Cuts at: 4414 4716
Size: 302
Fragments arranged by size:
2196 855 503 387 302 297 78 50
48
Bpu10I CC'TnA_GC
Cuts at: 0 1697 3976 4716
Size: 1697 2279 740
Fragments arranged by size:
2279 1697 740
BsaBI GATnn'nnATC
Cuts at: 0 4061 4716
Size: 4061 655
BsaHI Gr'CG_yC
Cuts at: 0 1853 2808 3953 4716
Size: 1853 955 1145 763
Fragments arranged by size:
1853 1145 955 763
BsaWI w'CCGG_w
Cuts at: 0 1734 3699 4328 4716
Size: 1734 1965 629 388
Fragments arranged by size:
1965 1734 629 388
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2128 2158 3955 3985 4716
Size: 2128 30 1797 30 731
Fragments arranged by size:
2128 1797 731 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 434 1546 1689 1692 1695 1714 2671 2683
Size: 434 1112 143 3 3 19 957 12
Cuts at: 2683 3685 3841 3910 3913 4516 4681 4716
Size: 1002 156 69 3 603 165 35
Fragments arranged by size:
1112 1002 957 603 434 165 156 143
69 35 19 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2447 2867 4716
Size: 2447 420 1849
Fragments arranged by size:
2447 1849 420
BsiEI CG_ry'CG
Cuts at: 0 855 899 3560 4716
Size: 855 44 2661 1156
Fragments arranged by size:
2661 1156 855 44
BsiHKAI G_wGCw'C
Cuts at: 0 398 552 820 1640 2306 3376 3480 4716
Size: 398 154 268 820 666 1070 104 1236
Fragments arranged by size:
1236 1070 820 666 398 268 154 104
BsmI GAATG_Cn'
Cuts at: 0 1511 1710 3643 4716
Size: 1511 199 1933 1073
Fragments arranged by size:
1933 1511 1073 199
BsmBI CGTCTCn'nnnn_
Cuts at: 0 405 1213 4716
Size: 405 808 3503
Fragments arranged by size:
3503 808 405
Bsp1286I G_dGCh'C
Cuts at: 0 289 322 390 398 465 552 820 842
Size: 289 33 68 8 67 87 268 22
Cuts at: 842 908 914 1640 1748 2092 2306 2388 2679
Size: 66 6 726 108 344 214 82 291
Cuts at: 2679 3376 3480 4452 4716
Size: 697 104 972 264
Fragments arranged by size:
972 726 697 344 291 289 268 264
214 108 104 87 82 68 67 66
33 22 8 6
BspHI T'CATG_A
Cuts at: 0 3233 4716
Size: 3233 1483
BsrBI CCG'CTC
Cuts at: 0 809 1035 1488 3636 4716
Size: 809 226 453 2148 1080
Fragments arranged by size:
2148 1080 809 453 226
BsrDI GCAATG_nn'
Cuts at: 0 2276 4500 4716
Size: 2276 2224 216
Fragments arranged by size:
2276 2224 216
BsrFI r'CCGG_y
Cuts at: 0 759 834 854 927 1804 3776 4716
Size: 759 75 20 73 877 1972 940
Fragments arranged by size:
1972 940 877 759 75 73 20
BssSI C'ACGA_G
Cuts at: 0 3631 4716
Size: 3631 1085
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 831 4716
Size: 216 615 3885
Fragments arranged by size:
3885 615 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1437 4716
Size: 1437 3279
BstYI r'GATC_y
Cuts at: 0 274 1415 1894 1986 2627 2954 3346 3456
Size: 274 1141 479 92 641 327 392 110
Cuts at: 3456 3548 4062 4399 4406 4716
Size: 92 514 337 7 310
Fragments arranged by size:
1141 641 514 479 392 337 327 310
274 110 92 92 7
BstZ17I GTA'TAC
Cuts at: 0 238 4716
Size: 238 4478
Bsu36I CC'TnA_GG
Cuts at: 0 3144 4716
Size: 3144 1572
BtgI C'CryG_G
Cuts at: 0 893 1016 1130 2108 4716
Size: 893 123 114 978 2608
Fragments arranged by size:
2608 978 893 123 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 40 4676 4716
Size: 40 4636 40
Fragments arranged by size:
4636 40 40
BtsI GCAGTG_nn'
Cuts at: 0 908 1442 2577 3976 4716
Size: 908 534 1135 1399 740
Fragments arranged by size:
1399 1135 908 740 534
ClaI AT'CG_AT
Cuts at: 0 230 4172 4716
Size: 230 3942 544
Fragments arranged by size:
3942 544 230
DraIII CAC_nnn'GTG
Cuts at: 0 2613 3204 4716
Size: 2613 591 1512
Fragments arranged by size:
2613 1512 591
DrdI GACnn_nn'nnGTC
Cuts at: 0 180 1780 4423 4716
Size: 180 1600 2643 293
Fragments arranged by size:
2643 1600 293 180
EaeI y'GGCC_r
Cuts at: 0 852 896 1406 3557 4716
Size: 852 44 510 2151 1159
Fragments arranged by size:
2151 1159 852 510 44
EagI C'GGCC_G
Cuts at: 0 852 896 3557 4716
Size: 852 44 2661 1159
Fragments arranged by size:
2661 1159 852 44
EarI CTCTTCn'nnn_
Cuts at: 0 1666 1901 1925 1940 1964 3887 4007 4090
Size: 1666 235 24 15 24 1923 120 83
Cuts at: 4090 4095 4469 4493 4502 4517 4716
Size: 5 374 24 9 15 199
Fragments arranged by size:
1923 1666 374 235 199 120 83 24
24 24 15 15 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3795 4716
Size: 3795 921
EcoICRI GAG'CTC
Cuts at: 0 550 3478 4716
Size: 550 2928 1238
Fragments arranged by size:
2928 1238 550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 437 940 1033 1237 1315 1544 2170
Size: 387 50 503 93 204 78 229 626
Cuts at: 2170 2869 3326 3346 3388 3887 4366 4414 4716
Size: 699 457 20 42 499 479 48 302
Fragments arranged by size:
699 626 503 499 479 457 387 302
229 204 93 78 50 48 42 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 89 308 4348 4716
Size: 89 219 4040 368
Fragments arranged by size:
4040 368 219 89
EcoO109I rG'GnC_Cy
Cuts at: 0 40 52 319 461 462 508 911 2165
Size: 40 12 267 142 1 46 403 1254
Cuts at: 2165 2190 2642 2676 3920 4716
Size: 25 452 34 1244 796
Fragments arranged by size:
1254 1244 796 452 403 267 142 46
40 34 25 12 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4042 4716
Size: 4042 674
FseI GG_CCGG'CC
Cuts at: 0 858 4716
Size: 858 3858
FspI TGC'GCA
Cuts at: 0 1201 4716
Size: 1201 3515
HaeII r_GCGC'y
Cuts at: 0 1260 3933 4716
Size: 1260 2673 783
Fragments arranged by size:
2673 1260 783
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2128 2160 2916 2948 3213 3245 3604 3636
Size: 2128 32 756 32 265 32 359 32
Cuts at: 3636 4111 4143 4302 4334 4391 4423 4516 4528
Size: 475 32 159 32 57 32 93 12
Cuts at: 4528 4548 4560 4716
Size: 20 12 156
Fragments arranged by size:
2128 756 475 359 265 159 156 93
57 32 32 32 32 32 32 32
20 12 12
HincII GTy'rAC
Cuts at: 0 771 4716
Size: 771 3945
HpaI GTT'AAC
Cuts at: 0 771 4716
Size: 771 3945
MslI CAynn'nnrTG
Cuts at: 0 292 2545 3025 4716
Size: 292 2253 480 1691
Fragments arranged by size:
2253 1691 480 292
MspA1I CmG'CkG
Cuts at: 0 534 895 960 1173 1848 2649 3412 4117
Size: 534 361 65 213 675 801 763 705
Cuts at: 4117 4716
Size: 599
Fragments arranged by size:
801 763 705 675 599 534 361 213
65
NaeI GCC'GGC
Cuts at: 0 761 856 929 4716
Size: 761 95 73 3787
Fragments arranged by size:
3787 761 95 73
NdeI CA'TA_TG
Cuts at: 0 2367 2939 4716
Size: 2367 572 1777
Fragments arranged by size:
2367 1777 572
NgoMIV G'CCGG_C
Cuts at: 0 759 854 927 4716
Size: 759 95 73 3789
Fragments arranged by size:
3789 759 95 73
NotI GC'GGCC_GC
Cuts at: 0 896 3557 4716
Size: 896 2661 1159
Fragments arranged by size:
2661 1159 896
NsiI A_TGCA'T
Cuts at: 0 212 221 4716
Size: 212 9 4495
Fragments arranged by size:
4495 212 9
NspI r_CATG'y
Cuts at: 0 210 1200 1375 1453 2073 2910 3609 4716
Size: 210 990 175 78 620 837 699 1107
Fragments arranged by size:
1107 990 837 699 620 210 175 78
PasI CC'CwG_GG
Cuts at: 0 37 67 1728 4716
Size: 37 30 1661 2988
Fragments arranged by size:
2988 1661 37 30
PciI A'CATG_T
Cuts at: 0 2906 4716
Size: 2906 1810
PflMI CCAn_nnn'nTGG
Cuts at: 0 79 142 2193 4716
Size: 79 63 2051 2523
Fragments arranged by size:
2523 2051 79 63
PfoI T'CCnGG_A
Cuts at: 0 90 141 1982 3844 4716
Size: 90 51 1841 1862 872
Fragments arranged by size:
1862 1841 872 90 51
PpuMI rG'GwC_Cy
Cuts at: 0 508 2165 2642 3920 4716
Size: 508 1657 477 1278 796
Fragments arranged by size:
1657 1278 796 508 477
PshAI GACnn'nnGTC
Cuts at: 0 2593 4716
Size: 2593 2123
PsiI TTA'TAA
Cuts at: 0 566 2500 4716
Size: 566 1934 2216
Fragments arranged by size:
2216 1934 566
PspOMI G'GGCC_C
Cuts at: 0 318 461 910 2675 4716
Size: 318 143 449 1765 2041
Fragments arranged by size:
2041 1765 449 318 143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3259 3291 4716
Size: 3259 32 1425
Fragments arranged by size:
3259 1425 32
PstI C_TGCA'G
Cuts at: 0 608 965 2654 2719 2921 3464 3653 4716
Size: 608 357 1689 65 202 543 189 1063
Fragments arranged by size:
1689 1063 608 543 357 202 189 65
PvuII CAG'CTG
Cuts at: 0 534 960 1173 1848 3412 4716
Size: 534 426 213 675 1564 1304
Fragments arranged by size:
1564 1304 675 534 426 213
SacI G_AGCT'C
Cuts at: 0 552 3480 4716
Size: 552 2928 1236
Fragments arranged by size:
2928 1236 552
SacII CC_GC'GG
Cuts at: 0 896 4716
Size: 896 3820
SanDI GG'GwC_CC
Cuts at: 0 2165 2642 3920 4716
Size: 2165 477 1278 796
Fragments arranged by size:
2165 1278 796 477
SbfI CC_TGCA'GG
Cuts at: 0 3653 4716
Size: 3653 1063
SfcI C'TryA_G
Cuts at: 0 604 961 2650 2715 2917 3460 3649 3945
Size: 604 357 1689 65 202 543 189 296
Cuts at: 3945 4640 4716
Size: 695 76
Fragments arranged by size:
1689 695 604 543 357 296 202 189
76 65
SmaI CCC'GGG
Cuts at: 0 794 909 935 4716
Size: 794 115 26 3781
Fragments arranged by size:
3781 794 115 26
SmlI C'TyrA_G
Cuts at: 0 4027 4716
Size: 4027 689
SphI G_CATG'C
Cuts at: 0 210 1200 1453 2073 4716
Size: 210 990 253 620 2643
Fragments arranged by size:
2643 990 620 253 210
SrfI GCCC'GGGC
Cuts at: 0 909 4716
Size: 909 3807
SspI AAT'ATT
Cuts at: 0 3908 4716
Size: 3908 808
StuI AGG'CCT
Cuts at: 0 1007 4716
Size: 1007 3709
StyI C'CwwG_G
Cuts at: 0 351 2948 3096 4335 4716
Size: 351 2597 148 1239 381
Fragments arranged by size:
2597 1239 381 351 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 50 323 386 1300 1388 1612 1848 3068
Size: 50 273 63 914 88 224 236 1220
Cuts at: 3068 3488 4716
Size: 420 1228
Fragments arranged by size:
1228 1220 914 420 273 236 224 88
63 50
TatI w'GTAC_w
Cuts at: 0 2075 4716
Size: 2075 2641
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2985 3017 3955 3987 4716
Size: 2985 32 938 32 729
Fragments arranged by size:
2985 938 729 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1128 2455 4716
Size: 1128 1327 2261
Fragments arranged by size:
2261 1327 1128
XmaI C'CCGG_G
Cuts at: 0 792 907 933 4716
Size: 792 115 26 3783
Fragments arranged by size:
3783 792 115 26
XmnI GAAnn'nnTTC
Cuts at: 0 3331 4310 4716
Size: 3331 979 406
Fragments arranged by size:
3331 979 406
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlfI AlwNI ApaI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII
HpaI MslI MspA1I NaeI NdeI NgoMIV NotI NsiI
NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuII SacI SacII SanDI SbfI
SfcI SmaI SmlI SphI SrfI SspI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AloI
ApaLI AscI AsiSI BbeI BmtI BpuEI BsaI BsaAI
BsgI BsiWI BspEI BspMI BsrGI BssHII BstBI BstEII
CspCI DraI EcoRI EcoRV FspAI HindIII KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NheI NruI
PacI PmeI PmlI PpiI PspXI PvuI RsrII SalI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SwaI Tth111I XbaI XhoI ZraI