Restriction map for
pFN21AE0789
(Linear) (Six-base) MAPSORT of: KIEE0789.seq Check: 9977 from: 1 to: 1062
KIEE0789.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AccI GT'mk_AC
Cuts at: 0 602 1062
Size: 602 460
AgeI A'CCGG_T
Cuts at: 0 719 1062
Size: 719 343
AlwNI CAG_nnn'CTG
Cuts at: 0 253 329 765 928 1062
Size: 253 76 436 163 134
Fragments arranged by size:
436 253 163 134 76
ApaI G_GGCC'C
Cuts at: 0 164 532 790 1062
Size: 164 368 258 272
Fragments arranged by size:
368 272 258 164
AscI GG'CGCG_CC
Cuts at: 0 98 1062
Size: 98 964
AvaI C'yCGr_G
Cuts at: 0 649 972 1062
Size: 649 323 90
Fragments arranged by size:
649 323 90
BanI G'GyrC_C
Cuts at: 0 85 117 313 412 645 828 868 1033
Size: 85 32 196 99 233 183 40 165
Cuts at: 1033 1062
Size: 29
Fragments arranged by size:
233 196 183 165 99 85 40 32
29
BanII G_rGCy'C
Cuts at: 0 13 55 164 532 790 1062
Size: 13 42 109 368 258 272
Fragments arranged by size:
368 272 258 109 42 13
BbeI G_GCGC'C
Cuts at: 0 89 121 317 416 649 872 1037 1062
Size: 89 32 196 99 233 223 165 25
Fragments arranged by size:
233 223 196 165 99 89 32 25
BglI GCCn_nnn'nGGC
Cuts at: 0 159 238 866 977 1062
Size: 159 79 628 111 85
Fragments arranged by size:
628 159 111 85 79
BglII A'GATC_T
Cuts at: 0 701 1062
Size: 701 361
Bme1580I G_kGCm'C
Cuts at: 0 164 532 790 1062
Size: 164 368 258 272
Fragments arranged by size:
368 272 258 164
BmgBI CAC'GTC
Cuts at: 0 1058 1062
Size: 1058 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 631 955 1062
Size: 429 202 324 107
Fragments arranged by size:
429 324 202 107
Bpu10I CC'TnA_GC
Cuts at: 0 49 929 1062
Size: 49 880 133
Fragments arranged by size:
880 133 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 58 1062
Size: 58 1004
BsaHI Gr'CG_yC
Cuts at: 0 25 86 118 293 314 413 512 646
Size: 25 61 32 175 21 99 99 134
Cuts at: 646 685 869 919 1025 1034 1062
Size: 39 184 50 106 9 28
Fragments arranged by size:
184 175 134 106 99 99 61 50
39 32 28 25 21 9
BsaWI w'CCGG_w
Cuts at: 0 719 1062
Size: 719 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 928 958 1062
Size: 928 30 104
Fragments arranged by size:
928 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 156 952 1062
Size: 76 80 796 110
Fragments arranged by size:
796 110 80 76
BseYI C'CCAG_C
Cuts at: 0 525 949 1062
Size: 525 424 113
Fragments arranged by size:
525 424 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 949 1062
Size: 520 429 113
Fragments arranged by size:
520 429 113
BsiEI CG_ry'CG
Cuts at: 0 137 182 233 269 1004 1062
Size: 137 45 51 36 735 58
Fragments arranged by size:
735 137 58 51 45 36
BsiHKAI G_wGCw'C
Cuts at: 0 584 662 1062
Size: 584 78 400
Fragments arranged by size:
584 400 78
BsmBI CGTCTCn'nnnn_
Cuts at: 0 300 1062
Size: 300 762
Bsp1286I G_dGCh'C
Cuts at: 0 13 55 164 532 584 662 790 1062
Size: 13 42 109 368 52 78 128 272
Fragments arranged by size:
368 272 128 109 78 52 42 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 588 684 1049 1062
Size: 588 96 365 13
Fragments arranged by size:
588 365 96 13
BsrBI CCG'CTC
Cuts at: 0 559 615 1062
Size: 559 56 447
Fragments arranged by size:
559 447 56
BsrFI r'CCGG_y
Cuts at: 0 400 481 499 719 895 909 1062
Size: 400 81 18 220 176 14 153
Fragments arranged by size:
400 220 176 153 81 18 14
BssHII G'CGCG_C
Cuts at: 0 98 146 1062
Size: 98 48 916
Fragments arranged by size:
916 98 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 253 329 765 857 1062
Size: 253 76 436 92 205
Fragments arranged by size:
436 253 205 92 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 525 1062
Size: 453 72 537
Fragments arranged by size:
537 453 72
BstYI r'GATC_y
Cuts at: 0 701 1062
Size: 701 361
BtgI C'CryG_G
Cuts at: 0 89 131 182 209 233 317 1062
Size: 89 42 51 27 24 84 745
Fragments arranged by size:
745 89 84 51 42 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 489 1062
Size: 489 573
EaeI y'GGCC_r
Cuts at: 0 134 230 266 395 479 665 888 1001
Size: 134 96 36 129 84 186 223 113
Cuts at: 1001 1062
Size: 61
Fragments arranged by size:
223 186 134 129 113 96 84 61
36
EagI C'GGCC_G
Cuts at: 0 134 230 266 1001 1062
Size: 134 96 36 735 61
Fragments arranged by size:
735 134 96 61 36
EarI CTCTTCn'nnn_
Cuts at: 0 589 1062
Size: 589 473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 791 1062
Size: 791 271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 631 955 1062
Size: 429 202 324 107
Fragments arranged by size:
429 324 202 107
EcoO109I rG'GnC_Cy
Cuts at: 0 161 1062
Size: 161 901
HaeII r_GCGC'y
Cuts at: 0 89 121 317 416 576 621 649 872
Size: 89 32 196 99 160 45 28 223
Cuts at: 872 1037 1062
Size: 165 25
Fragments arranged by size:
223 196 165 160 99 89 45 32
28 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 928 960 1062
Size: 928 32 102
Fragments arranged by size:
928 102 32
KasI G'GCGC_C
Cuts at: 0 85 117 313 412 645 868 1033 1062
Size: 85 32 196 99 233 223 165 29
Fragments arranged by size:
233 223 196 165 99 85 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 1062
Size: 65 997
MscI TGG'CCA
Cuts at: 0 667 1062
Size: 667 395
MspA1I CmG'CkG
Cuts at: 0 83 133 235 253 534 765 1019 1062
Size: 83 50 102 18 281 231 254 43
Fragments arranged by size:
281 254 231 102 83 50 43 18
NaeI GCC'GGC
Cuts at: 0 483 911 1062
Size: 483 428 151
Fragments arranged by size:
483 428 151
NarI GG'CG_CC
Cuts at: 0 86 118 314 413 646 869 1034 1062
Size: 86 32 196 99 233 223 165 28
Fragments arranged by size:
233 223 196 165 99 86 32 28
NcoI C'CATG_G
Cuts at: 0 89 209 317 1062
Size: 89 120 108 745
Fragments arranged by size:
745 120 108 89
NgoMIV G'CCGG_C
Cuts at: 0 481 909 1062
Size: 481 428 153
Fragments arranged by size:
481 428 153
NotI GC'GGCC_GC
Cuts at: 0 134 230 266 1001 1062
Size: 134 96 36 735 61
Fragments arranged by size:
735 134 96 61 36
NspI r_CATG'y
Cuts at: 0 195 285 384 1062
Size: 195 90 99 678
Fragments arranged by size:
678 195 99 90
PasI CC'CwG_GG
Cuts at: 0 123 1062
Size: 123 939
PflMI CCAn_nnn'nTGG
Cuts at: 0 404 1062
Size: 404 658
PspOMI G'GGCC_C
Cuts at: 0 160 528 786 1062
Size: 160 368 258 276
Fragments arranged by size:
368 276 258 160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 706 738 1062
Size: 706 32 324
Fragments arranged by size:
706 324 32
PstI C_TGCA'G
Cuts at: 0 770 1062
Size: 770 292
PvuII CAG'CTG
Cuts at: 0 253 765 1062
Size: 253 512 297
Fragments arranged by size:
512 297 253
RsrII CG'GwC_CG
Cuts at: 0 898 1062
Size: 898 164
SacII CC_GC'GG
Cuts at: 0 134 236 1062
Size: 134 102 826
Fragments arranged by size:
826 134 102
SapI GCTCTTCn'nnn_
Cuts at: 0 589 1062
Size: 589 473
SfcI C'TryA_G
Cuts at: 0 766 1062
Size: 766 296
SfoI GGC'GCC
Cuts at: 0 87 119 315 414 647 870 1035 1062
Size: 87 32 196 99 233 223 165 27
Fragments arranged by size:
233 223 196 165 99 87 32 27
SmaI CCC'GGG
Cuts at: 0 974 1062
Size: 974 88
SphI G_CATG'C
Cuts at: 0 195 384 1062
Size: 195 189 678
Fragments arranged by size:
678 195 189
StyI C'CwwG_G
Cuts at: 0 89 209 317 743 1062
Size: 89 120 108 426 319
Fragments arranged by size:
426 319 120 108 89
TatI w'GTAC_w
Cuts at: 0 1013 1062
Size: 1013 49
XmaI C'CCGG_G
Cuts at: 0 972 1062
Size: 972 90
Enzymes that do cut:
AccI AgeI AlwNI ApaI AscI AvaI BanI BanII
BbeI BglI BglII Bme1580I BmgBI BpmI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI
BstYI BtgI BtgZI EaeI EagI EarI EciI Eco57MI
EcoO109I HaeII Hin4I KasI MmeI MscI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PflMI PspOMI
PsrI PstI PvuII RsrII SacII SapI SfcI SfoI
SmaI SphI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MslI
NdeI NheI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PvuI SacI
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI