Restriction map for
pFN21AE0766
(Linear) (Six-base) MAPSORT of: KIEE0766.seq Check: 2171 from: 1 to: 1314
KIEE0766.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AccI GT'mk_AC
Cuts at: 0 773 845 917 989 1061 1133 1205 1314
Size: 773 72 72 72 72 72 72 109
Fragments arranged by size:
773 109 72 72 72 72 72 72
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1314
Size: 343 971
AleI CACnn'nnGTG
Cuts at: 0 1227 1314
Size: 1227 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 1314
Size: 127 1187
ApaI G_GGCC'C
Cuts at: 0 334 1314
Size: 334 980
ApoI r'AATT_y
Cuts at: 0 732 804 876 948 1020 1092 1164 1314
Size: 732 72 72 72 72 72 72 150
Fragments arranged by size:
732 150 72 72 72 72 72 72
BanII G_rGCy'C
Cuts at: 0 334 1314
Size: 334 980
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 1314
Size: 53 1261
Bme1580I G_kGCm'C
Cuts at: 0 334 1234 1314
Size: 334 900 80
Fragments arranged by size:
900 334 80
BmgBI CAC'GTC
Cuts at: 0 270 379 1314
Size: 270 109 935
Fragments arranged by size:
935 270 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 1314
Size: 51 1263
BmtI G_CTAG'C
Cuts at: 0 80 1314
Size: 80 1234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 1314
Size: 24 1290
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 389 392 1314
Size: 389 3 922
Fragments arranged by size:
922 389 3
BseYI C'CCAG_C
Cuts at: 0 86 327 1314
Size: 86 241 987
Fragments arranged by size:
987 241 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1314
Size: 391 923
BsiHKAI G_wGCw'C
Cuts at: 0 363 1314
Size: 363 951
Bsp1286I G_dGCh'C
Cuts at: 0 334 363 1234 1314
Size: 334 29 871 80
Fragments arranged by size:
871 334 80 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 1314
Size: 461 853
BstAPI GCAn_nnn'nTGC
Cuts at: 0 721 793 865 937 1009 1081 1153 1314
Size: 721 72 72 72 72 72 72 161
Fragments arranged by size:
721 161 72 72 72 72 72 72
BstEII G'GTnAC_C
Cuts at: 0 603 819 963 1107 1179 1314
Size: 603 216 144 144 72 135
Fragments arranged by size:
603 216 144 144 135 72
BstZ17I GTA'TAC
Cuts at: 0 774 846 918 990 1062 1134 1206 1314
Size: 774 72 72 72 72 72 72 108
Fragments arranged by size:
774 108 72 72 72 72 72 72
BtgI C'CryG_G
Cuts at: 0 309 1314
Size: 309 1005
BtsI GCAGTG_nn'
Cuts at: 0 677 1234 1314
Size: 677 557 80
Fragments arranged by size:
677 557 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 365 400 1314
Size: 365 35 914
Fragments arranged by size:
914 365 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 274 1314
Size: 274 1040
EarI CTCTTCn'nnn_
Cuts at: 0 1302 1314
Size: 1302 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 444 1314
Size: 444 870
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 343 1314
Size: 24 319 971
Fragments arranged by size:
971 319 24
EcoO109I rG'GnC_Cy
Cuts at: 0 331 1314
Size: 331 983
EcoRV GAT'ATC
Cuts at: 0 616 688 760 832 904 976 1048 1120
Size: 616 72 72 72 72 72 72 72
Cuts at: 1120 1192 1314
Size: 72 122
Fragments arranged by size:
616 122 72 72 72 72 72 72
72 72
MslI CAynn'nnrTG
Cuts at: 0 308 1227 1275 1314
Size: 308 919 48 39
Fragments arranged by size:
919 308 48 39
MspA1I CmG'CkG
Cuts at: 0 70 1314
Size: 70 1244
NcoI C'CATG_G
Cuts at: 0 309 1314
Size: 309 1005
NheI G'CTAG_C
Cuts at: 0 76 1314
Size: 76 1238
PspOMI G'GGCC_C
Cuts at: 0 330 1314
Size: 330 984
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 174 206 1314
Size: 174 32 1108
Fragments arranged by size:
1108 174 32
PstI C_TGCA'G
Cuts at: 0 75 458 1314
Size: 75 383 856
Fragments arranged by size:
856 383 75
PvuII CAG'CTG
Cuts at: 0 70 1314
Size: 70 1244
SexAI A'CCwGG_T
Cuts at: 0 522 1314
Size: 522 792
SfcI C'TryA_G
Cuts at: 0 71 454 1314
Size: 71 383 860
Fragments arranged by size:
860 383 71
StyI C'CwwG_G
Cuts at: 0 81 309 1314
Size: 81 228 1005
Fragments arranged by size:
1005 228 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 78 1279 1314
Size: 70 8 1201 35
Fragments arranged by size:
1201 70 35 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 459 491 1314
Size: 459 32 823
Fragments arranged by size:
823 459 32
Enzymes that do cut:
AccI AcuI AleI AlfI ApaI ApoI BanII BbsI
Bme1580I BmgBI BmrI BmtI BpmI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BstAPI BstEII BstZ17I BtgI BtsI
CspCI DrdI EarI EciI Eco57MI EcoO109I EcoRV MslI
MspA1I NcoI NheI PspOMI PsrI PstI PvuII SexAI
SfcI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstXI BstYI Bsu36I BtgZI ClaI
DraI DraIII EaeI EagI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI