Restriction map for
pFN21AE0765
(Linear) (Six-base) MAPSORT of: KIEE0765.seq Check: 623 from: 1 to: 1017
KIEE0765.orf
With 182 enzymes: SgfI *
March 22, 2012 15:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 964 1017
Size: 524 440 53
Fragments arranged by size:
524 440 53
AflIII A'CryG_T
Cuts at: 0 522 1017
Size: 522 495
ApoI r'AATT_y
Cuts at: 0 290 719 965 1017
Size: 290 429 246 52
Fragments arranged by size:
429 290 246 52
AseI AT'TA_AT
Cuts at: 0 26 667 1017
Size: 26 641 350
Fragments arranged by size:
641 350 26
BanI G'GyrC_C
Cuts at: 0 791 1017
Size: 791 226
BanII G_rGCy'C
Cuts at: 0 322 1017
Size: 322 695
BbsI GAAGACnn'nnnn_
Cuts at: 0 273 543 757 1017
Size: 273 270 214 260
Fragments arranged by size:
273 270 260 214
BfrBI ATG'CAT
Cuts at: 0 913 1017
Size: 913 104
BglII A'GATC_T
Cuts at: 0 247 1017
Size: 247 770
BlpI GC'TnA_GC
Cuts at: 0 254 316 321 1017
Size: 254 62 5 696
Fragments arranged by size:
696 254 62 5
Bme1580I G_kGCm'C
Cuts at: 0 796 1017
Size: 796 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1017
Size: 502 515
BsaBI GATnn'nnATC
Cuts at: 0 406 680 1017
Size: 406 274 337
Fragments arranged by size:
406 337 274
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 551 1017
Size: 551 466
BsiHKAI G_wGCw'C
Cuts at: 0 95 260 322 1017
Size: 95 165 62 695
Fragments arranged by size:
695 165 95 62
Bsp1286I G_dGCh'C
Cuts at: 0 95 260 322 796 1017
Size: 95 165 62 474 221
Fragments arranged by size:
474 221 165 95 62
BsrGI T'GTAC_A
Cuts at: 0 77 808 1017
Size: 77 731 209
Fragments arranged by size:
731 209 77
BstXI CCAn_nnnn'nTGG
Cuts at: 0 176 1017
Size: 176 841
BstYI r'GATC_y
Cuts at: 0 247 607 1017
Size: 247 360 410
Fragments arranged by size:
410 360 247
BtgI C'CryG_G
Cuts at: 0 191 1017
Size: 191 826
BtsI GCAGTG_nn'
Cuts at: 0 528 1017
Size: 528 489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 96 131 833 868 1017
Size: 96 35 702 35 149
Fragments arranged by size:
702 149 96 35 35
EaeI y'GGCC_r
Cuts at: 0 194 1017
Size: 194 823
EarI CTCTTCn'nnn_
Cuts at: 0 500 1017
Size: 500 517
EcoICRI GAG'CTC
Cuts at: 0 320 1017
Size: 320 697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 524 964 1017
Size: 502 22 440 53
Fragments arranged by size:
502 440 53 22
HaeII r_GCGC'y
Cuts at: 0 503 1017
Size: 503 514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1017
Size: 421 596
MscI TGG'CCA
Cuts at: 0 196 1017
Size: 196 821
MslI CAynn'nnrTG
Cuts at: 0 987 1017
Size: 987 30
NcoI C'CATG_G
Cuts at: 0 191 1017
Size: 191 826
NsiI A_TGCA'T
Cuts at: 0 915 1017
Size: 915 102
NspI r_CATG'y
Cuts at: 0 526 1017
Size: 526 491
PciI A'CATG_T
Cuts at: 0 522 1017
Size: 522 495
PstI C_TGCA'G
Cuts at: 0 535 1017
Size: 535 482
SacI G_AGCT'C
Cuts at: 0 322 1017
Size: 322 695
SfcI C'TryA_G
Cuts at: 0 531 1017
Size: 531 486
SspI AAT'ATT
Cuts at: 0 30 1017
Size: 30 987
StyI C'CwwG_G
Cuts at: 0 191 925 1017
Size: 191 734 92
Fragments arranged by size:
734 191 92
TatI w'GTAC_w
Cuts at: 0 77 808 1017
Size: 77 731 209
Fragments arranged by size:
731 209 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 1017
Size: 198 819
Enzymes that do cut:
AcuI AflIII ApoI AseI BanI BanII BbsI BfrBI
BglII BlpI Bme1580I BpmI BsaBI BseRI BsiHKAI Bsp1286I
BsrGI BstXI BstYI BtgI BtsI CspCI EaeI EarI
EcoICRI Eco57MI HaeII MmeI MscI MslI NcoI NsiI
NspI PciI PstI SacI SfcI SspI StyI TatI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI XmnI ZraI