Restriction map for
pFN21AE0761
(Linear) (Six-base) MAPSORT of: KIEE0761.seq Check: 8022 from: 1 to: 1011
KIEE0761.orf
With 182 enzymes: SgfI *
October 11, 2011 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 80 467 558 1011
Size: 80 387 91 453
Fragments arranged by size:
453 387 91 80
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1011
Size: 984 27
AlwNI CAG_nnn'CTG
Cuts at: 0 866 1011
Size: 866 145
ApaI G_GGCC'C
Cuts at: 0 428 1011
Size: 428 583
AvaI C'yCGr_G
Cuts at: 0 540 688 938 1011
Size: 540 148 250 73
Fragments arranged by size:
540 250 148 73
BanII G_rGCy'C
Cuts at: 0 287 335 428 547 910 1011
Size: 287 48 93 119 363 101
Fragments arranged by size:
363 287 119 101 93 48
BbvCI CC'TCA_GC
Cuts at: 0 629 1011
Size: 629 382
BglI GCCn_nnn'nGGC
Cuts at: 0 21 1011
Size: 21 990
Bme1580I G_kGCm'C
Cuts at: 0 378 428 1011
Size: 378 50 583
Fragments arranged by size:
583 378 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 1011
Size: 381 630
Bpu10I CC'TnA_GC
Cuts at: 0 629 904 1011
Size: 629 275 107
Fragments arranged by size:
629 275 107
BsaAI yAC'GTr
Cuts at: 0 741 1011
Size: 741 270
BsaHI Gr'CG_yC
Cuts at: 0 182 1011
Size: 182 829
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 328 748 1011
Size: 295 33 420 263
Fragments arranged by size:
420 295 263 33
BseYI C'CCAG_C
Cuts at: 0 154 323 788 1011
Size: 154 169 465 223
Fragments arranged by size:
465 223 169 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1011
Size: 58 953
BsiEI CG_ry'CG
Cuts at: 0 25 920 1011
Size: 25 895 91
Fragments arranged by size:
895 91 25
BsiHKAI G_wGCw'C
Cuts at: 0 287 740 814 1011
Size: 287 453 74 197
Fragments arranged by size:
453 287 197 74
BsmBI CGTCTCn'nnnn_
Cuts at: 0 193 1011
Size: 193 818
Bsp1286I G_dGCh'C
Cuts at: 0 287 335 378 428 547 740 814 910
Size: 287 48 43 50 119 193 74 96
Cuts at: 910 1011
Size: 101
Fragments arranged by size:
287 193 119 101 96 74 50 48
43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 467 500 558 836 1011
Size: 80 387 33 58 278 175
Fragments arranged by size:
387 278 175 80 58 33
BsrBI CCG'CTC
Cuts at: 0 530 560 893 1011
Size: 530 30 333 118
Fragments arranged by size:
530 333 118 30
BsrFI r'CCGG_y
Cuts at: 0 290 382 581 1011
Size: 290 92 199 430
Fragments arranged by size:
430 290 199 92
BssHII G'CGCG_C
Cuts at: 0 150 960 962 1011
Size: 150 810 2 49
Fragments arranged by size:
810 150 49 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 1011
Size: 796 215
BstYI r'GATC_y
Cuts at: 0 876 1011
Size: 876 135
Bsu36I CC'TnA_GG
Cuts at: 0 430 473 1011
Size: 430 43 538
Fragments arranged by size:
538 430 43
BtgI C'CryG_G
Cuts at: 0 93 911 1011
Size: 93 818 100
Fragments arranged by size:
818 100 93
BtsI GCAGTG_nn'
Cuts at: 0 867 1011
Size: 867 144
DraIII CAC_nnn'GTG
Cuts at: 0 213 1011
Size: 213 798
DrdI GACnn_nn'nnGTC
Cuts at: 0 166 1011
Size: 166 845
EaeI y'GGCC_r
Cuts at: 0 2 22 516 638 917 1011
Size: 2 20 494 122 279 94
Fragments arranged by size:
494 279 122 94 20 2
EagI C'GGCC_G
Cuts at: 0 22 917 1011
Size: 22 895 94
Fragments arranged by size:
895 94 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 277 1011
Size: 277 734
EcoICRI GAG'CTC
Cuts at: 0 285 1011
Size: 285 726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1011
Size: 984 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 169 463 1011
Size: 169 294 548
Fragments arranged by size:
548 294 169
EcoO109I rG'GnC_Cy
Cuts at: 0 425 469 748 1011
Size: 425 44 279 263
Fragments arranged by size:
425 279 263 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1011
Size: 529 32 450
Fragments arranged by size:
529 450 32
HincII GTy'rAC
Cuts at: 0 177 186 954 1011
Size: 177 9 768 57
Fragments arranged by size:
768 177 57 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1011
Size: 863 148
MscI TGG'CCA
Cuts at: 0 640 1011
Size: 640 371
MslI CAynn'nnrTG
Cuts at: 0 592 1011
Size: 592 419
MspA1I CmG'CkG
Cuts at: 0 18 95 300 1011
Size: 18 77 205 711
Fragments arranged by size:
711 205 77 18
NaeI GCC'GGC
Cuts at: 0 292 1011
Size: 292 719
NgoMIV G'CCGG_C
Cuts at: 0 290 1011
Size: 290 721
NotI GC'GGCC_GC
Cuts at: 0 22 1011
Size: 22 989
PasI CC'CwG_GG
Cuts at: 0 353 1011
Size: 353 658
PflMI CCAn_nnn'nTGG
Cuts at: 0 213 1011
Size: 213 798
PfoI T'CCnGG_A
Cuts at: 0 884 1011
Size: 884 127
PmlI CAC'GTG
Cuts at: 0 741 1011
Size: 741 270
PpuMI rG'GwC_Cy
Cuts at: 0 469 748 1011
Size: 469 279 263
Fragments arranged by size:
469 279 263
PspOMI G'GGCC_C
Cuts at: 0 424 1011
Size: 424 587
PspXI vC'TCGA_Gb
Cuts at: 0 938 1011
Size: 938 73
PvuII CAG'CTG
Cuts at: 0 300 1011
Size: 300 711
SacI G_AGCT'C
Cuts at: 0 287 1011
Size: 287 724
SacII CC_GC'GG
Cuts at: 0 96 1011
Size: 96 915
SexAI A'CCwGG_T
Cuts at: 0 209 1011
Size: 209 802
SgrAI Cr'CCGG_yG
Cuts at: 0 290 1011
Size: 290 721
SmaI CCC'GGG
Cuts at: 0 542 1011
Size: 542 469
SmlI C'TyrA_G
Cuts at: 0 938 1011
Size: 938 73
StyI C'CwwG_G
Cuts at: 0 926 1011
Size: 926 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 741 1011
Size: 741 270
TatI w'GTAC_w
Cuts at: 0 521 1011
Size: 521 490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 796 974 1011
Size: 796 178 37
Fragments arranged by size:
796 178 37
XhoI C'TCGA_G
Cuts at: 0 938 1011
Size: 938 73
XmaI C'CCGG_G
Cuts at: 0 540 1011
Size: 540 471
Enzymes that do cut:
AarI AcuI AlwNI ApaI AvaI BanII BbvCI BglI
Bme1580I BmrI Bpu10I BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I NaeI NgoMIV NotI PasI
PflMI PfoI PmlI PpuMI PspOMI PspXI PvuII SacI
SacII SexAI SgrAI SmaI SmlI StyI TaqII TatI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI EarI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NruI
NsiI NspI PacI PciI PmeI PpiI PshAI PsiI
PsrI PstI PvuI RsrII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XmnI
ZraI