Restriction map for
pFN21AE0704
(Linear) (Six-base) MAPSORT of: KIEE0704.seq Check: 5476 from: 1 to: 1221
KIEE0704.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 370 484 754 932 1221
Size: 305 65 114 270 178 289
Fragments arranged by size:
305 289 270 178 114 65
AleI CACnn'nnGTG
Cuts at: 0 1114 1221
Size: 1114 107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1081 1221
Size: 1081 140
AlwNI CAG_nnn'CTG
Cuts at: 0 450 1029 1221
Size: 450 579 192
Fragments arranged by size:
579 450 192
ApoI r'AATT_y
Cuts at: 0 405 646 688 1221
Size: 405 241 42 533
Fragments arranged by size:
533 405 241 42
AvaI C'yCGr_G
Cuts at: 0 25 1221
Size: 25 1196
BanI G'GyrC_C
Cuts at: 0 93 1221
Size: 93 1128
BbsI GAAGACnn'nnnn_
Cuts at: 0 854 1221
Size: 854 367
BbvCI CC'TCA_GC
Cuts at: 0 1045 1221
Size: 1045 176
BciVI GTATCCnnnnn_n'
Cuts at: 0 505 1221
Size: 505 716
BglII A'GATC_T
Cuts at: 0 210 1221
Size: 210 1011
BlpI GC'TnA_GC
Cuts at: 0 83 157 1221
Size: 83 74 1064
Fragments arranged by size:
1064 83 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 1039 1098 1221
Size: 1039 59 123
Fragments arranged by size:
1039 123 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 896 1221
Size: 896 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 632 976 1221
Size: 472 160 344 245
Fragments arranged by size:
472 344 245 160
Bpu10I CC'TnA_GC
Cuts at: 0 1045 1221
Size: 1045 176
BsaAI yAC'GTr
Cuts at: 0 1061 1221
Size: 1061 160
BsaHI Gr'CG_yC
Cuts at: 0 734 1221
Size: 734 487
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 881 1156 1221
Size: 881 275 65
Fragments arranged by size:
881 275 65
BseYI C'CCAG_C
Cuts at: 0 786 1022 1221
Size: 786 236 199
Fragments arranged by size:
786 236 199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 1221
Size: 815 406
BsmI GAATG_Cn'
Cuts at: 0 726 1221
Size: 726 495
BsrDI GCAATG_nn'
Cuts at: 0 147 1221
Size: 147 1074
BsrGI T'GTAC_A
Cuts at: 0 363 1221
Size: 363 858
BstXI CCAn_nnnn'nTGG
Cuts at: 0 586 1221
Size: 586 635
BstYI r'GATC_y
Cuts at: 0 210 1221
Size: 210 1011
BtsI GCAGTG_nn'
Cuts at: 0 1178 1221
Size: 1178 43
DrdI GACnn_nn'nnGTC
Cuts at: 0 733 1221
Size: 733 488
EaeI y'GGCC_r
Cuts at: 0 474 712 1152 1221
Size: 474 238 440 69
Fragments arranged by size:
474 440 238 69
EarI CTCTTCn'nnn_
Cuts at: 0 773 1221
Size: 773 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 370 472 484 632 754 932 976
Size: 305 65 102 12 148 122 178 44
Cuts at: 976 1221
Size: 245
Fragments arranged by size:
305 245 178 148 122 102 65 44
12
EcoO109I rG'GnC_Cy
Cuts at: 0 396 1012 1221
Size: 396 616 209
Fragments arranged by size:
616 396 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 807 839 876 908 963 995 1082 1114
Size: 807 32 37 32 55 32 87 32
Cuts at: 1114 1168 1200 1221
Size: 54 32 21
Fragments arranged by size:
807 87 55 54 37 32 32 32
32 32 21
MscI TGG'CCA
Cuts at: 0 476 714 1221
Size: 476 238 507
Fragments arranged by size:
507 476 238
MslI CAynn'nnrTG
Cuts at: 0 584 1063 1114 1221
Size: 584 479 51 107
Fragments arranged by size:
584 479 107 51
MspA1I CmG'CkG
Cuts at: 0 156 359 1151 1221
Size: 156 203 792 70
Fragments arranged by size:
792 203 156 70
NdeI CA'TA_TG
Cuts at: 0 69 234 1221
Size: 69 165 987
Fragments arranged by size:
987 165 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 1029 1221
Size: 1029 192
PfoI T'CCnGG_A
Cuts at: 0 913 1221
Size: 913 308
PpuMI rG'GwC_Cy
Cuts at: 0 396 1012 1221
Size: 396 616 209
Fragments arranged by size:
616 396 209
PstI C_TGCA'G
Cuts at: 0 1080 1221
Size: 1080 141
PvuII CAG'CTG
Cuts at: 0 156 1221
Size: 156 1065
SanDI GG'GwC_CC
Cuts at: 0 1012 1221
Size: 1012 209
SapI GCTCTTCn'nnn_
Cuts at: 0 773 1221
Size: 773 448
SfcI C'TryA_G
Cuts at: 0 1076 1221
Size: 1076 145
SmaI CCC'GGG
Cuts at: 0 27 1221
Size: 27 1194
StyI C'CwwG_G
Cuts at: 0 681 715 1221
Size: 681 34 506
Fragments arranged by size:
681 506 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 1221
Size: 592 629
TatI w'GTAC_w
Cuts at: 0 115 363 1106 1221
Size: 115 248 743 115
Fragments arranged by size:
743 248 115 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 677 709 1221
Size: 677 32 512
Fragments arranged by size:
677 512 32
XmaI C'CCGG_G
Cuts at: 0 25 1221
Size: 25 1196
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApoI AvaI BanI BbsI
BbvCI BciVI BglII BlpI BmrI BplI BpmI Bpu10I
BsaAI BsaHI BseRI BseYI BsgI BsmI BsrDI BsrGI
BstXI BstYI BtsI DrdI EaeI EarI Eco57MI EcoO109I
Hin4I MscI MslI MspA1I NdeI PflMI PfoI PpuMI
PstI PvuII SanDI SapI SfcI SmaI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BcgI BclI BfrBI
BglI Bme1580I BmgBI BmtI BpuEI BsaI BsaBI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI