Restriction map for
pFN21AE0697
(Linear) (Six-base) MAPSORT of: KIEE0697.seq Check: 804 from: 1 to: 2538
KIEE0697.orf
With 182 enzymes: SgfI *
February 18, 2010 15:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1543 2538
Size: 1543 995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2421 2437 2538
Size: 2421 16 101
Fragments arranged by size:
2421 101 16
AflIII A'CryG_T
Cuts at: 0 1472 2538
Size: 1472 1066
AhdI GACnn_n'nnGTC
Cuts at: 0 1250 2098 2538
Size: 1250 848 440
Fragments arranged by size:
1250 848 440
AleI CACnn'nnGTG
Cuts at: 0 487 1138 1987 2538
Size: 487 651 849 551
Fragments arranged by size:
849 651 551 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 778 2538
Size: 778 1760
AlwNI CAG_nnn'CTG
Cuts at: 0 21 366 798 1020 1179 2050 2460 2538
Size: 21 345 432 222 159 871 410 78
Fragments arranged by size:
871 432 410 345 222 159 78 21
ApaI G_GGCC'C
Cuts at: 0 273 907 1501 2538
Size: 273 634 594 1037
Fragments arranged by size:
1037 634 594 273
ApoI r'AATT_y
Cuts at: 0 449 2538
Size: 449 2089
AvaI C'yCGr_G
Cuts at: 0 1739 2538
Size: 1739 799
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2239 2272 2538
Size: 2239 33 266
Fragments arranged by size:
2239 266 33
BamHI G'GATC_C
Cuts at: 0 351 2538
Size: 351 2187
BanI G'GyrC_C
Cuts at: 0 582 810 965 1482 1543 1704 2538
Size: 582 228 155 517 61 161 834
Fragments arranged by size:
834 582 517 228 161 155 61
BanII G_rGCy'C
Cuts at: 0 273 907 1173 1501 1903 2138 2538
Size: 273 634 266 328 402 235 400
Fragments arranged by size:
634 402 400 328 273 266 235
BbeI G_GCGC'C
Cuts at: 0 969 2538
Size: 969 1569
BbsI GAAGACnn'nnnn_
Cuts at: 0 135 236 1462 1818 2296 2538
Size: 135 101 1226 356 478 242
Fragments arranged by size:
1226 478 356 242 135 101
BbvCI CC'TCA_GC
Cuts at: 0 1734 2538
Size: 1734 804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1137 1171 2538
Size: 1137 34 1367
Fragments arranged by size:
1367 1137 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1674 2538
Size: 1674 864
BclI T'GATC_A
Cuts at: 0 1424 1649 2538
Size: 1424 225 889
Fragments arranged by size:
1424 889 225
BfrBI ATG'CAT
Cuts at: 0 1003 2538
Size: 1003 1535
BglI GCCn_nnn'nGGC
Cuts at: 0 703 2374 2412 2538
Size: 703 1671 38 126
Fragments arranged by size:
1671 703 126 38
BglII A'GATC_T
Cuts at: 0 1370 1430 2538
Size: 1370 60 1108
Fragments arranged by size:
1370 1108 60
BlpI GC'TnA_GC
Cuts at: 0 1172 2203 2538
Size: 1172 1031 335
Fragments arranged by size:
1172 1031 335
Bme1580I G_kGCm'C
Cuts at: 0 273 427 437 907 1487 1501 2273 2538
Size: 273 154 10 470 580 14 772 265
Fragments arranged by size:
772 580 470 273 265 154 14 10
BmgBI CAC'GTC
Cuts at: 0 2198 2538
Size: 2198 340
BmrI ACTGGGnnnn_n'
Cuts at: 0 1771 2538
Size: 1771 767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1392 1431 2538
Size: 1392 39 1107
Fragments arranged by size:
1392 1107 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 138 212 317 325 409 535 583
Size: 76 62 74 105 8 84 126 48
Cuts at: 583 855 1864 2538
Size: 272 1009 674
Fragments arranged by size:
1009 674 272 126 105 84 76 74
62 48 8
Bpu10I CC'TnA_GC
Cuts at: 0 1734 1880 2538
Size: 1734 146 658
Fragments arranged by size:
1734 658 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 1237 2538
Size: 172 1065 1301
Fragments arranged by size:
1301 1065 172
BsaBI GATnn'nnATC
Cuts at: 0 1429 2538
Size: 1429 1109
BsaHI Gr'CG_yC
Cuts at: 0 966 2538
Size: 966 1572
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2440 2470 2538
Size: 2440 30 68
Fragments arranged by size:
2440 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 271 372 1510 1722 2509 2538
Size: 73 198 101 1138 212 787 29
Fragments arranged by size:
1138 787 212 198 101 73 29
BseYI C'CCAG_C
Cuts at: 0 44 528 609 641 1164 1745 2538
Size: 44 484 81 32 523 581 793
Fragments arranged by size:
793 581 523 484 81 44 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 838 1034 1162 2538
Size: 712 126 196 128 1376
Fragments arranged by size:
1376 712 196 128 126
BsiHKAI G_wGCw'C
Cuts at: 0 1535 2538
Size: 1535 1003
BsmI GAATG_Cn'
Cuts at: 0 1003 1148 2538
Size: 1003 145 1390
Fragments arranged by size:
1390 1003 145
Bsp1286I G_dGCh'C
Cuts at: 0 273 427 437 907 1173 1487 1501 1535
Size: 273 154 10 470 266 314 14 34
Cuts at: 1535 1903 2138 2273 2538
Size: 368 235 135 265
Fragments arranged by size:
470 368 314 273 266 265 235 154
135 34 14 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 1928 2538
Size: 179 1749 610
Fragments arranged by size:
1749 610 179
BsrBI CCG'CTC
Cuts at: 0 2430 2538
Size: 2430 108
BsrFI r'CCGG_y
Cuts at: 0 522 579 1540 2364 2538
Size: 522 57 961 824 174
Fragments arranged by size:
961 824 522 174 57
BssHII G'CGCG_C
Cuts at: 0 40 2538
Size: 40 2498
BstAPI GCAn_nnn'nTGC
Cuts at: 0 525 798 2538
Size: 525 273 1740
Fragments arranged by size:
1740 525 273
BstBI TT'CG_AA
Cuts at: 0 834 2538
Size: 834 1704
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2130 2538
Size: 2130 408
BstYI r'GATC_y
Cuts at: 0 236 351 1370 1430 2060 2538
Size: 236 115 1019 60 630 478
Fragments arranged by size:
1019 630 478 236 115 60
Bsu36I CC'TnA_GG
Cuts at: 0 245 617 1493 2538
Size: 245 372 876 1045
Fragments arranged by size:
1045 876 372 245
BtsI GCAGTG_nn'
Cuts at: 0 796 1133 1145 2345 2538
Size: 796 337 12 1200 193
Fragments arranged by size:
1200 796 337 193 12
ClaI AT'CG_AT
Cuts at: 0 632 2538
Size: 632 1906
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1748 1783 1848 1883 2538
Size: 1748 35 65 35 655
Fragments arranged by size:
1748 655 65 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1630 2538
Size: 1630 908
EaeI y'GGCC_r
Cuts at: 0 197 648 1538 1949 2525 2538
Size: 197 451 890 411 576 13
Fragments arranged by size:
890 576 451 411 197 13
EarI CTCTTCn'nnn_
Cuts at: 0 406 943 1159 2538
Size: 406 537 216 1379
Fragments arranged by size:
1379 537 406 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1794 2538
Size: 1794 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 138 212 317 325 409 535 583
Size: 76 62 74 105 8 84 126 48
Cuts at: 583 855 1864 2421 2437 2538
Size: 272 1009 557 16 101
Fragments arranged by size:
1009 557 272 126 105 101 84 76
74 62 48 16 8
EcoO109I rG'GnC_Cy
Cuts at: 0 80 270 309 613 621 885 903 945
Size: 80 190 39 304 8 264 18 42
Cuts at: 945 1017 1201 1497 1498 1632 2538
Size: 72 184 296 1 134 906
Fragments arranged by size:
906 304 296 264 190 184 134 80
72 42 39 18 8 1
EcoRI G'AATT_C
Cuts at: 0 449 2538
Size: 449 2089
EcoRV GAT'ATC
Cuts at: 0 960 2538
Size: 960 1578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2502 2538
Size: 2502 36
FspI TGC'GCA
Cuts at: 0 1570 2538
Size: 1570 968
HaeII r_GCGC'y
Cuts at: 0 843 969 2538
Size: 843 126 1569
Fragments arranged by size:
1569 843 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 769 801 1236 1268 2084 2116
Size: 228 32 509 32 435 32 816 32
Cuts at: 2116 2538
Size: 422
Fragments arranged by size:
816 509 435 422 228 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 965 2538
Size: 965 1573
KpnI G_GTAC'C
Cuts at: 0 1547 2538
Size: 1547 991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1751 2538
Size: 1097 654 787
Fragments arranged by size:
1097 787 654
MscI TGG'CCA
Cuts at: 0 199 2527 2538
Size: 199 2328 11
Fragments arranged by size:
2328 199 11
MslI CAynn'nnrTG
Cuts at: 0 487 667 1138 1789 1987 2023 2128 2264
Size: 487 180 471 651 198 36 105 136
Cuts at: 2264 2533 2538
Size: 269 5
Fragments arranged by size:
651 487 471 269 198 180 136 105
36 5
MspA1I CmG'CkG
Cuts at: 0 556 2082 2207 2409 2538
Size: 556 1526 125 202 129
Fragments arranged by size:
1526 556 202 129 125
NaeI GCC'GGC
Cuts at: 0 2366 2538
Size: 2366 172
NarI GG'CG_CC
Cuts at: 0 966 2538
Size: 966 1572
NgoMIV G'CCGG_C
Cuts at: 0 2364 2538
Size: 2364 174
NsiI A_TGCA'T
Cuts at: 0 1005 2538
Size: 1005 1533
NspI r_CATG'y
Cuts at: 0 2304 2538
Size: 2304 234
PasI CC'CwG_GG
Cuts at: 0 312 2044 2538
Size: 312 1732 494
Fragments arranged by size:
1732 494 312
PflMI CCAn_nnn'nTGG
Cuts at: 0 1020 1761 2538
Size: 1020 741 777
Fragments arranged by size:
1020 777 741
PfoI T'CCnGG_A
Cuts at: 0 512 746 1436 1841 2063 2538
Size: 512 234 690 405 222 475
Fragments arranged by size:
690 512 475 405 234 222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 2538
Size: 667 32 1839
Fragments arranged by size:
1839 667 32
PpuMI rG'GwC_Cy
Cuts at: 0 613 1017 1201 1632 2538
Size: 613 404 184 431 906
Fragments arranged by size:
906 613 431 404 184
PsiI TTA'TAA
Cuts at: 0 1463 2538
Size: 1463 1075
PspOMI G'GGCC_C
Cuts at: 0 269 903 1497 2538
Size: 269 634 594 1041
Fragments arranged by size:
1041 634 594 269
PstI C_TGCA'G
Cuts at: 0 333 548 757 923 1140 2055 2538
Size: 333 215 209 166 217 915 483
Fragments arranged by size:
915 483 333 217 215 209 166
SanDI GG'GwC_CC
Cuts at: 0 1632 2538
Size: 1632 906
SapI GCTCTTCn'nnn_
Cuts at: 0 406 943 2538
Size: 406 537 1595
Fragments arranged by size:
1595 537 406
SfcI C'TryA_G
Cuts at: 0 329 544 753 919 1136 1450 2051 2538
Size: 329 215 209 166 217 314 601 487
Fragments arranged by size:
601 487 329 314 217 215 209 166
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2374 2538
Size: 2374 164
SfoI GGC'GCC
Cuts at: 0 967 2538
Size: 967 1571
SmaI CCC'GGG
Cuts at: 0 1741 2538
Size: 1741 797
SphI G_CATG'C
Cuts at: 0 2304 2538
Size: 2304 234
StyI C'CwwG_G
Cuts at: 0 149 2477 2538
Size: 149 2328 61
Fragments arranged by size:
2328 149 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 36 266 655 1460 1705 1772 1930 2017
Size: 36 230 389 805 245 67 158 87
Cuts at: 2017 2265 2276 2337 2538
Size: 248 11 61 201
Fragments arranged by size:
805 389 248 245 230 201 158 87
67 61 36 11
TatI w'GTAC_w
Cuts at: 0 1259 2163 2538
Size: 1259 904 375
Fragments arranged by size:
1259 904 375
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2440 2472 2538
Size: 2440 32 66
Fragments arranged by size:
2440 66 32
XbaI T'CTAG_A
Cuts at: 0 1254 2538
Size: 1254 1284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1650 2538
Size: 1650 888
XmaI C'CCGG_G
Cuts at: 0 1739 2538
Size: 1739 799
XmnI GAAnn'nnTTC
Cuts at: 0 461 2538
Size: 461 2077
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI
Bsu36I BtsI ClaI CspCI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI
PsiI PspOMI PstI SanDI SapI SfcI SfiI SfoI
SmaI SphI StyI TaqII TatI TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BmtI BpuEI BsaAI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstEII BstZ17I BtgI BtgZI DraI DraIII EagI
EcoICRI EcoNI FseI FspAI HincII HindIII HpaI MfeI
MluI NcoI NdeI NheI NotI NruI PacI PciI
PmeI PmlI PshAI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI SexAI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XhoI ZraI