Restriction map for
pFN21AE0662
(Linear) (Six-base) MAPSORT of: KIEE0662.seq Check: 9726 from: 1 to: 1161
KIEE0662.orf
With 182 enzymes: SgfI *
July 3, 2012 16:40 ..
AatII G_ACGT'C
Cuts at: 0 158 1161
Size: 158 1003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 235 289 472 879 926 1161
Size: 159 76 54 183 407 47 235
Fragments arranged by size:
407 235 183 159 76 54 47
AflIII A'CryG_T
Cuts at: 0 635 1161
Size: 635 526
AgeI A'CCGG_T
Cuts at: 0 160 1161
Size: 160 1001
AlwNI CAG_nnn'CTG
Cuts at: 0 708 1161
Size: 708 453
ApaLI G'TGCA_C
Cuts at: 0 439 1161
Size: 439 722
AvaI C'yCGr_G
Cuts at: 0 5 457 612 754 902 986 1161
Size: 5 452 155 142 148 84 175
Fragments arranged by size:
452 175 155 148 142 84 5
BanI G'GyrC_C
Cuts at: 0 248 692 1161
Size: 248 444 469
Fragments arranged by size:
469 444 248
BanII G_rGCy'C
Cuts at: 0 65 335 399 1161
Size: 65 270 64 762
Fragments arranged by size:
762 270 65 64
BbvCI CC'TCA_GC
Cuts at: 0 715 1161
Size: 715 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 533 567 1161
Size: 533 34 594
Fragments arranged by size:
594 533 34
BclI T'GATC_A
Cuts at: 0 722 1161
Size: 722 439
BfrBI ATG'CAT
Cuts at: 0 110 1161
Size: 110 1051
BglI GCCn_nnn'nGGC
Cuts at: 0 690 1161
Size: 690 471
Bme1580I G_kGCm'C
Cuts at: 0 251 443 1161
Size: 251 192 718
Fragments arranged by size:
718 251 192
BmrI ACTGGGnnnn_n'
Cuts at: 0 557 1080 1161
Size: 557 523 81
Fragments arranged by size:
557 523 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 349 1161
Size: 349 812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 544 1161
Size: 424 120 617
Fragments arranged by size:
617 424 120
Bpu10I CC'TnA_GC
Cuts at: 0 715 1161
Size: 715 446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1161
Size: 384 777
BsaI GGTCTCn'nnnn_
Cuts at: 0 246 277 998 1161
Size: 246 31 721 163
Fragments arranged by size:
721 246 163 31
BsaBI GATnn'nnATC
Cuts at: 0 997 1161
Size: 997 164
BsaHI Gr'CG_yC
Cuts at: 0 155 650 1043 1161
Size: 155 495 393 118
Fragments arranged by size:
495 393 155 118
BsaWI w'CCGG_w
Cuts at: 0 160 695 1161
Size: 160 535 466
Fragments arranged by size:
535 466 160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1126 1161
Size: 199 927 35
Fragments arranged by size:
927 199 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 399 1161
Size: 20 379 762
Fragments arranged by size:
762 379 20
BsiHKAI G_wGCw'C
Cuts at: 0 335 399 443 595 646 1136 1161
Size: 335 64 44 152 51 490 25
Fragments arranged by size:
490 335 152 64 51 44 25
Bsp1286I G_dGCh'C
Cuts at: 0 65 251 335 399 443 595 646 1136
Size: 65 186 84 64 44 152 51 490
Cuts at: 1136 1161
Size: 25
Fragments arranged by size:
490 186 152 84 65 64 51 44
25
BspHI T'CATG_A
Cuts at: 0 725 1161
Size: 725 436
BsrBI CCG'CTC
Cuts at: 0 731 1161
Size: 731 430
BsrFI r'CCGG_y
Cuts at: 0 160 673 1031 1161
Size: 160 513 358 130
Fragments arranged by size:
513 358 160 130
BsrGI T'GTAC_A
Cuts at: 0 659 1161
Size: 659 502
BssSI C'ACGA_G
Cuts at: 0 123 1161
Size: 123 1038
BstEII G'GTnAC_C
Cuts at: 0 357 1161
Size: 357 804
BstXI CCAn_nnnn'nTGG
Cuts at: 0 570 1161
Size: 570 591
BstYI r'GATC_y
Cuts at: 0 71 950 1161
Size: 71 879 211
Fragments arranged by size:
879 211 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 1161
Size: 120 1041
BtsI GCAGTG_nn'
Cuts at: 0 16 99 1161
Size: 16 83 1062
Fragments arranged by size:
1062 83 16
DraIII CAC_nnn'GTG
Cuts at: 0 1074 1161
Size: 1074 87
EaeI y'GGCC_r
Cuts at: 0 671 1065 1145 1161
Size: 671 394 80 16
Fragments arranged by size:
671 394 80 16
EarI CTCTTCn'nnn_
Cuts at: 0 316 833 1161
Size: 316 517 328
Fragments arranged by size:
517 328 316
EcoICRI GAG'CTC
Cuts at: 0 333 397 1161
Size: 333 64 764
Fragments arranged by size:
764 333 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 235 289 424 472 544 879 926
Size: 159 76 54 135 48 72 335 47
Cuts at: 926 1161
Size: 235
Fragments arranged by size:
335 235 159 135 76 72 54 48
47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 1147 1161
Size: 235 912 14
Fragments arranged by size:
912 235 14
EcoO109I rG'GnC_Cy
Cuts at: 0 447 557 786 1161
Size: 447 110 229 375
Fragments arranged by size:
447 375 229 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 1161
Size: 378 783
FspI TGC'GCA
Cuts at: 0 235 1161
Size: 235 926
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 1161
Size: 754 32 375
Fragments arranged by size:
754 375 32
MscI TGG'CCA
Cuts at: 0 1067 1161
Size: 1067 94
MspA1I CmG'CkG
Cuts at: 0 267 522 835 1161
Size: 267 255 313 326
Fragments arranged by size:
326 313 267 255
NaeI GCC'GGC
Cuts at: 0 675 1033 1161
Size: 675 358 128
Fragments arranged by size:
675 358 128
NgoMIV G'CCGG_C
Cuts at: 0 673 1031 1161
Size: 673 358 130
Fragments arranged by size:
673 358 130
NsiI A_TGCA'T
Cuts at: 0 112 1161
Size: 112 1049
NspI r_CATG'y
Cuts at: 0 639 1161
Size: 639 522
PciI A'CATG_T
Cuts at: 0 635 1161
Size: 635 526
PpuMI rG'GwC_Cy
Cuts at: 0 447 557 786 1161
Size: 447 110 229 375
Fragments arranged by size:
447 375 229 110
PvuII CAG'CTG
Cuts at: 0 267 522 1161
Size: 267 255 639
Fragments arranged by size:
639 267 255
SacI G_AGCT'C
Cuts at: 0 335 399 1161
Size: 335 64 762
Fragments arranged by size:
762 335 64
SanDI GG'GwC_CC
Cuts at: 0 557 1161
Size: 557 604
ScaI AGT'ACT
Cuts at: 0 871 1161
Size: 871 290
SexAI A'CCwGG_T
Cuts at: 0 788 1161
Size: 788 373
SgrAI Cr'CCGG_yG
Cuts at: 0 160 1161
Size: 160 1001
SmaI CCC'GGG
Cuts at: 0 7 614 904 988 1161
Size: 7 607 290 84 173
Fragments arranged by size:
607 290 173 84 7
SmlI C'TyrA_G
Cuts at: 0 363 1161
Size: 363 798
StuI AGG'CCT
Cuts at: 0 844 1161
Size: 844 317
StyI C'CwwG_G
Cuts at: 0 845 1124 1161
Size: 845 279 37
Fragments arranged by size:
845 279 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1062 1137 1161
Size: 1062 75 24
Fragments arranged by size:
1062 75 24
TatI w'GTAC_w
Cuts at: 0 659 869 1161
Size: 659 210 292
Fragments arranged by size:
659 292 210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 72 1161
Size: 72 1089
XmaI C'CCGG_G
Cuts at: 0 5 612 902 986 1161
Size: 5 607 290 84 175
Fragments arranged by size:
607 290 175 84 5
ZraI GAC'GTC
Cuts at: 0 156 1161
Size: 156 1005
Enzymes that do cut:
AatII AcuI AflIII AgeI AlwNI ApaLI AvaI BanI
BanII BbvCI BcgI BclI BfrBI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BseRI BsgI BsiHKAI Bsp1286I BspHI BsrBI BsrFI BsrGI
BssSI BstEII BstXI BstYI BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI Hin4I
MscI MspA1I NaeI NgoMIV NsiI NspI PciI PpuMI
PvuII SacI SanDI ScaI SexAI SgrAI SmaI SmlI
StuI StyI TaqII TatI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BciVI BglII BlpI BmgBI
BmtI BsaAI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DrdI EagI EciI EcoRI
EcoRV FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NarI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI SfcI SfiI
SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmnI