(Linear) (Six-base) MAPSORT of: KIEE0628.seq  Check: 2200  from: 1  to: 1116



KIEE0628.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:44  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     331    1116 
   Size:        331     785
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     932     983    1116 
   Size:        932      51     133

  Fragments arranged by size: 

                 932     133      51
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     873    1116 
   Size:        873     243
 
ApoI r'AATT_y

Cuts at:      0      59     127     184     227     350    1116 
   Size:         59      68      57      43     123     766

  Fragments arranged by size: 

                 766     123      68      59      57      43
 
AseI AT'TA_AT

Cuts at:      0     281    1116 
   Size:        281     835
 
AvaI C'yCGr_G

Cuts at:      0     514    1116 
   Size:        514     602
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     971    1019    1116 
   Size:        971      48      97

  Fragments arranged by size: 

                 971      97      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593    1116 
   Size:        593     523
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728    1116 
   Size:        728     388
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      36      66    1116 
   Size:         36      30    1050

  Fragments arranged by size: 

                1050      36      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      21      24      44    1116 
   Size:         21       3      20    1072

  Fragments arranged by size: 

                1072      21      20       3
 
BspHI T'CATG_A

Cuts at:      0     288    1116 
   Size:        288     828
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     331    1116 
   Size:        331     785
 
BsrGI T'GTAC_A

Cuts at:      0     462    1116 
   Size:        462     654
 
BssSI C'ACGA_G

Cuts at:      0     235    1116 
   Size:        235     881
 
BtsI GCAGTG_nn'

Cuts at:      0     889    1116 
   Size:        889     227
 
DraI TTT'AAA

Cuts at:      0     476    1116 
   Size:        476     640
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     215    1116 
   Size:        215     901
 
EaeI y'GGCC_r

Cuts at:      0     165     794    1116 
   Size:        165     629     322

  Fragments arranged by size: 

                 629     322     165
 
EarI CTCTTCn'nnn_

Cuts at:      0     198    1116 
   Size:        198     918
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593     932     983    1116 
   Size:        593     339      51     133

  Fragments arranged by size: 

                 593     339     133      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     956    1116 
   Size:        956     160
 
HincII GTy'rAC

Cuts at:      0     686    1116 
   Size:        686     430
 
HpaI GTT'AAC

Cuts at:      0     686    1116 
   Size:        686     430
 
MscI TGG'CCA

Cuts at:      0     167     796    1116 
   Size:        167     629     320

  Fragments arranged by size: 

                 629     320     167
 
MslI CAynn'nnrTG

Cuts at:      0     566    1116 
   Size:        566     550
 
MspA1I CmG'CkG

Cuts at:      0     792    1116 
   Size:        792     324
 
NspI r_CATG'y

Cuts at:      0     565    1116 
   Size:        565     551
 
PpuMI rG'GwC_Cy

Cuts at:      0     956    1116 
   Size:        956     160
 
PstI C_TGCA'G

Cuts at:      0     342    1116 
   Size:        342     774
 
PvuII CAG'CTG

Cuts at:      0     792    1116 
   Size:        792     324
 
SfcI C'TryA_G

Cuts at:      0     338     877    1116 
   Size:        338     539     239

  Fragments arranged by size: 

                 539     338     239
 
SmlI C'TyrA_G

Cuts at:      0     743    1116 
   Size:        743     373
 
SphI G_CATG'C

Cuts at:      0     565    1116 
   Size:        565     551
 
SspI AAT'ATT

Cuts at:      0     601     660    1116 
   Size:        601      59     456

  Fragments arranged by size: 

                 601     456      59
 
StuI AGG'CCT

Cuts at:      0      38    1116 
   Size:         38    1078
 
TatI w'GTAC_w

Cuts at:      0     462    1116 
   Size:        462     654
 
XmnI GAAnn'nnTTC

Cuts at:      0      30    1116 
   Size:         30    1086
 

 Enzymes that do cut:

     AarI     AcuI     AlfI     ApoI     AseI     AvaI     BbsI     BpmI
    BpuEI    BsaXI    BseRI    BspHI    BspMI    BsrGI    BssSI     BtsI
     DraI     DrdI     EaeI     EarI  Eco57MI EcoO109I   HincII     HpaI
     MscI     MslI   MspA1I     NspI    PpuMI     PstI    PvuII     SfcI
     SmlI     SphI     SspI     StuI     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI