(Linear) (Six-base) MAPSORT of: KIEE0609.seq  Check: 8996  from: 1  to: 513



KIEE0609.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     226     513 
   Size:        226     287
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     364     513 
   Size:        156     208     149

  Fragments arranged by size: 

                 208     156     149
 
AflIII A'CryG_T

Cuts at:      0      35     373     513 
   Size:         35     338     140

  Fragments arranged by size: 

                 338     140      35
 
AlwNI CAG_nnn'CTG

Cuts at:      0      72     513 
   Size:         72     441
 
ApoI r'AATT_y

Cuts at:      0     403     513 
   Size:        403     110
 
BmgBI CAC'GTC

Cuts at:      0      36     513 
   Size:         36     477
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     513 
   Size:         76     437
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     513 
   Size:        133     380
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     513 
   Size:         73     440
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     513 
   Size:        254     259
 
BsiHKAI G_wGCw'C

Cuts at:      0     400     513 
   Size:        400     113
 
BsmI GAATG_Cn'

Cuts at:      0     322     454     513 
   Size:        322     132      59

  Fragments arranged by size: 

                 322     132      59
 
Bsp1286I G_dGCh'C

Cuts at:      0     400     513 
   Size:        400     113
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     226     513 
   Size:        226     287
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     257     513 
   Size:        257     256
 
BtgI C'CryG_G

Cuts at:      0     137     513 
   Size:        137     376
 
DraI TTT'AAA

Cuts at:      0     471     513 
   Size:        471      42
 
DraIII CAC_nnn'GTG

Cuts at:      0     132     513 
   Size:        132     381
 
EaeI y'GGCC_r

Cuts at:      0       2     513 
   Size:          2     511
 
EarI CTCTTCn'nnn_

Cuts at:      0     185     513 
   Size:        185     328
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     156     364     513 
   Size:         76      80     208     149

  Fragments arranged by size: 

                 208     149      80      76
 
EcoO109I rG'GnC_Cy

Cuts at:      0     215     513 
   Size:        215     298
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     322     513 
   Size:        322     191
 
MscI TGG'CCA

Cuts at:      0       4     513 
   Size:          4     509
 
NspI r_CATG'y

Cuts at:      0     377     513 
   Size:        377     136
 
PciI A'CATG_T

Cuts at:      0     373     513 
   Size:        373     140
 
PfoI T'CCnGG_A

Cuts at:      0     196     513 
   Size:        196     317
 
PpuMI rG'GwC_Cy

Cuts at:      0     215     513 
   Size:        215     298
 
SanDI GG'GwC_CC

Cuts at:      0     215     513 
   Size:        215     298
 
SexAI A'CCwGG_T

Cuts at:      0     128     183     513 
   Size:        128      55     330

  Fragments arranged by size: 

                 330     128      55
 
SfcI C'TryA_G

Cuts at:      0      96     120     513 
   Size:         96      24     393

  Fragments arranged by size: 

                 393      96      24
 
SmlI C'TyrA_G

Cuts at:      0     112     513 
   Size:        112     401
 
SspI AAT'ATT

Cuts at:      0     436     513 
   Size:        436      77
 
StyI C'CwwG_G

Cuts at:      0     310     513 
   Size:        310     203
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     118     150     513 
   Size:        118      32     363

  Fragments arranged by size: 

                 363     118      32
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApoI    BmgBI     BpmI    BpuEI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BstXI     BtgI
     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I     FalI     MscI
     NspI     PciI     PfoI    PpuMI    SanDI    SexAI     SfcI     SmlI
     SspI     StyI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI