Restriction map for
pFN21AE0600
(Linear) (Six-base) MAPSORT of: KIEE0600.seq Check: 969 from: 1 to: 15900
KIEE0600.orf
With 182 enzymes: SgfI *
May 13, 2010 15:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1452 5415 6204 9603 14718 15900
Size: 1452 3963 789 3399 5115 1182
Fragments arranged by size:
5115 3963 3399 1452 1182 789
AccI GT'mk_AC
Cuts at: 0 722 3592 4315 6417 6912 8948 9300 9938
Size: 722 2870 723 2102 495 2036 352 638
Cuts at: 9938 10441 11507 15900
Size: 503 1066 4393
Fragments arranged by size:
4393 2870 2102 2036 1066 723 722 638
503 495 352
AclI AA'CG_TT
Cuts at: 0 247 15900
Size: 247 15653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 1760 2020 2358 2962 4115 4466 4570
Size: 1519 241 260 338 604 1153 351 104
Cuts at: 4570 4579 5063 5422 5440 6857 6925 7099 8059
Size: 9 484 359 18 1417 68 174 960
Cuts at: 8059 8336 8651 9308 9580 10852 10914 11522 11732
Size: 277 315 657 272 1272 62 608 210
Cuts at: 11732 11999 12085 12700 13486 13918 14114 14702 15761
Size: 267 86 615 786 432 196 588 1059
Cuts at: 15761 15900
Size: 139
Fragments arranged by size:
1519 1417 1272 1153 1059 960 786 657
615 608 604 588 484 432 359 351
338 315 277 272 267 260 241 210
196 174 139 104 86 68 62 18
9
AfeI AGC'GCT
Cuts at: 0 3730 15789 15900
Size: 3730 12059 111
Fragments arranged by size:
12059 3730 111
AflII C'TTAA_G
Cuts at: 0 4161 12639 15900
Size: 4161 8478 3261
Fragments arranged by size:
8478 4161 3261
AflIII A'CryG_T
Cuts at: 0 4278 4948 4950 7049 7568 10957 11336 14920
Size: 4278 670 2 2099 519 3389 379 3584
Cuts at: 14920 15900
Size: 980
Fragments arranged by size:
4278 3584 3389 2099 980 670 519 379
2
AgeI A'CCGG_T
Cuts at: 0 1395 15182 15900
Size: 1395 13787 718
Fragments arranged by size:
13787 1395 718
AhdI GACnn_n'nnGTC
Cuts at: 0 509 796 5641 11900 12954 15887 15900
Size: 509 287 4845 6259 1054 2933 13
Fragments arranged by size:
6259 4845 2933 1054 509 287 13
AleI CACnn'nnGTG
Cuts at: 0 865 10940 12263 15900
Size: 865 10075 1323 3637
Fragments arranged by size:
10075 3637 1323 865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 1385 1849 3275 4550 6299 6786 8223
Size: 385 1000 464 1426 1275 1749 487 1437
Cuts at: 8223 12946 15900
Size: 4723 2954
Fragments arranged by size:
4723 2954 1749 1437 1426 1275 1000 487
464 385
AlwNI CAG_nnn'CTG
Cuts at: 0 1479 2043 3037 3093 4407 4665 5106 5352
Size: 1479 564 994 56 1314 258 441 246
Cuts at: 5352 5364 5884 7006 7176 7678 7821 7903 7986
Size: 12 520 1122 170 502 143 82 83
Cuts at: 7986 8976 10177 11117 13896 13971 15900
Size: 990 1201 940 2779 75 1929
Fragments arranged by size:
2779 1929 1479 1314 1201 1122 994 990
940 564 520 502 441 258 246 170
143 83 82 75 56 12
ApaI G_GGCC'C
Cuts at: 0 7550 11070 15900
Size: 7550 3520 4830
Fragments arranged by size:
7550 4830 3520
ApaLI G'TGCA_C
Cuts at: 0 13804 15900
Size: 13804 2096
ApoI r'AATT_y
Cuts at: 0 1088 1156 1944 2050 3209 3630 4012 5271
Size: 1088 68 788 106 1159 421 382 1259
Cuts at: 5271 5455 6790 7293 7304 7382 7612 7934 9254
Size: 184 1335 503 11 78 230 322 1320
Cuts at: 9254 11728 13072 13080 13093 13302 14176 14800 15037
Size: 2474 1344 8 13 209 874 624 237
Cuts at: 15037 15274 15337 15900
Size: 237 63 563
Fragments arranged by size:
2474 1344 1335 1320 1259 1159 1088 874
788 624 563 503 421 382 322 237
237 230 209 184 106 78 68 63
13 11 8
AseI AT'TA_AT
Cuts at: 0 9637 15900
Size: 9637 6263
AvaI C'yCGr_G
Cuts at: 0 374 1709 3787 5136 5542 11132 11705 14867
Size: 374 1335 2078 1349 406 5590 573 3162
Cuts at: 14867 15413 15900
Size: 546 487
Fragments arranged by size:
5590 3162 2078 1349 1335 573 546 487
406 374
AvrII C'CTAG_G
Cuts at: 0 13638 15900
Size: 13638 2262
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 10519 10552 15900
Size: 10519 33 5348
Fragments arranged by size:
10519 5348 33
BamHI G'GATC_C
Cuts at: 0 8550 9588 11930 14414 15900
Size: 8550 1038 2342 2484 1486
Fragments arranged by size:
8550 2484 2342 1486 1038
BanI G'GyrC_C
Cuts at: 0 2030 2742 3767 10632 15625 15900
Size: 2030 712 1025 6865 4993 275
Fragments arranged by size:
6865 4993 2030 1025 712 275
BanII G_rGCy'C
Cuts at: 0 1900 4876 6442 6590 6658 7550 8390 8913
Size: 1900 2976 1566 148 68 892 840 523
Cuts at: 8913 9833 11070 11111 11376 12399 12524 14307 14393
Size: 920 1237 41 265 1023 125 1783 86
Cuts at: 14393 15900
Size: 1507
Fragments arranged by size:
2976 1900 1783 1566 1507 1237 1023 920
892 840 523 265 148 125 86 68
41
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 1525 2480 2999 6611 6742 8090 8495
Size: 863 662 955 519 3612 131 1348 405
Cuts at: 8495 10088 13537 15865 15900
Size: 1593 3449 2328 35
Fragments arranged by size:
3612 3449 2328 1593 1348 955 863 662
519 405 131 35
BbvCI CC'TCA_GC
Cuts at: 0 2940 9029 10468 13790 15900
Size: 2940 6089 1439 3322 2110
Fragments arranged by size:
6089 3322 2940 2110 1439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2992 3026 3676 3710 10198 10232 14092 14126
Size: 2992 34 650 34 6488 34 3860 34
Cuts at: 14126 15900
Size: 1774
Fragments arranged by size:
6488 3860 2992 1774 650 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3826 13656 15900
Size: 3826 9830 2244
Fragments arranged by size:
9830 3826 2244
BclI T'GATC_A
Cuts at: 0 2714 8657 11169 13295 15900
Size: 2714 5943 2512 2126 2605
Fragments arranged by size:
5943 2714 2605 2512 2126
BfrBI ATG'CAT
Cuts at: 0 3262 5523 6763 15900
Size: 3262 2261 1240 9137
Fragments arranged by size:
9137 3262 2261 1240
BglI GCCn_nnn'nGGC
Cuts at: 0 4721 7276 15900
Size: 4721 2555 8624
Fragments arranged by size:
8624 4721 2555
BglII A'GATC_T
Cuts at: 0 312 542 3564 4466 7026 8852 15900
Size: 312 230 3022 902 2560 1826 7048
Fragments arranged by size:
7048 3022 2560 1826 902 312 230
BlpI GC'TnA_GC
Cuts at: 0 3946 4870 5954 6101 9805 10121 10862 11110
Size: 3946 924 1084 147 3704 316 741 248
Cuts at: 11110 14333 14387 14498 15900
Size: 3223 54 111 1402
Fragments arranged by size:
3946 3704 3223 1402 1084 924 741 316
248 147 111 54
Bme1580I G_kGCm'C
Cuts at: 0 7550 10637 11070 11139 13808 15900
Size: 7550 3087 433 69 2669 2092
Fragments arranged by size:
7550 3087 2669 2092 433 69
BmgBI CAC'GTC
Cuts at: 0 9261 14544 14923 15900
Size: 9261 5283 379 977
Fragments arranged by size:
9261 5283 977 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 802 2292 5283 6933 7803 9966 10527 11394
Size: 802 1490 2991 1650 870 2163 561 867
Cuts at: 11394 13259 13876 15900
Size: 1865 617 2024
Fragments arranged by size:
2991 2163 2024 1865 1650 1490 870 867
802 617 561
BmtI G_CTAG'C
Cuts at: 0 5147 15900
Size: 5147 10753
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 119 372 1914 5151 7176 9162 10634 15900
Size: 119 253 1542 3237 2025 1986 1472 5266
Fragments arranged by size:
5266 3237 2025 1986 1542 1472 253 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 824 924 1456 1828 2002 2142 2206
Size: 687 137 100 532 372 174 140 64
Cuts at: 2206 2413 2467 3199 3598 4258 4687 4921 4990
Size: 207 54 732 399 660 429 234 69
Cuts at: 4990 5533 5893 5921 6181 7700 7705 7810 8098
Size: 543 360 28 260 1519 5 105 288
Cuts at: 8098 8369 8917 9124 9199 9493 9889 10036 10659
Size: 271 548 207 75 294 396 147 623
Cuts at: 10659 11144 11476 11938 12641 13948 14767 14806 14847
Size: 485 332 462 703 1307 819 39 41
Cuts at: 14847 15426 15473 15795 15869 15900
Size: 579 47 322 74 31
Fragments arranged by size:
1519 1307 819 732 703 687 660 623
579 548 543 532 485 462 429 399
396 372 360 332 322 294 288 271
260 234 207 207 174 147 140 137
105 100 75 74 69 64 54 47
41 39 31 28 5
Bpu10I CC'TnA_GC
Cuts at: 0 2940 4916 5297 6252 7658 8785 9029 10468
Size: 2940 1976 381 955 1406 1127 244 1439
Cuts at: 10468 11182 13270 13790 15900
Size: 714 2088 520 2110
Fragments arranged by size:
2940 2110 2088 1976 1439 1406 1127 955
714 520 381 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1884 2263 2424 4130 5634 6652 8011
Size: 1163 721 379 161 1706 1504 1018 1359
Cuts at: 8011 8428 8858 8869 12079 14047 15900
Size: 417 430 11 3210 1968 1853
Fragments arranged by size:
3210 1968 1853 1706 1504 1359 1163 1018
721 430 417 379 161 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 1450 3445 4351 5539 7918 9346 12477 12491
Size: 1450 1995 906 1188 2379 1428 3131 14
Cuts at: 12491 13217 14422 15900
Size: 726 1205 1478
Fragments arranged by size:
3131 2379 1995 1478 1450 1428 1205 1188
906 726 14
BsaAI yAC'GTr
Cuts at: 0 3490 4206 4879 4951 15900
Size: 3490 716 673 72 10949
Fragments arranged by size:
10949 3490 716 673 72
BsaBI GATnn'nnATC
Cuts at: 0 5769 14293 15071 15900
Size: 5769 8524 778 829
Fragments arranged by size:
8524 5769 829 778
BsaHI Gr'CG_yC
Cuts at: 0 4233 15900
Size: 4233 11667
BsaWI w'CCGG_w
Cuts at: 0 1395 1580 2021 6794 15182 15900
Size: 1395 185 441 4773 8388 718
Fragments arranged by size:
8388 4773 1395 718 441 185
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 643 673 1903 1933 1975 2005 6154 6184
Size: 643 30 1230 30 42 30 4149 30
Cuts at: 6184 7027 7057 8278 8308 12667 12697 12985 13015
Size: 843 30 1221 30 4359 30 288 30
Cuts at: 13015 14640 14670 15900
Size: 1625 30 1230
Fragments arranged by size:
4359 4149 1625 1230 1230 1221 843 643
288 42 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1725 2113 2116 2203 3073 3139 3196 4255
Size: 1725 388 3 87 870 66 57 1059
Cuts at: 4255 4258 4261 4279 5353 5863 7153 9508 9841
Size: 3 3 18 1074 510 1290 2355 333
Cuts at: 9841 10330 10495 10498 10981 11371 11617 12712 14329
Size: 489 165 3 483 390 246 1095 1617
Cuts at: 14329 14533 14645 14803 15900
Size: 204 112 158 1097
Fragments arranged by size:
2355 1725 1617 1290 1097 1095 1074 1059
870 510 489 483 390 388 333 246
204 165 158 112 87 66 57 18
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1893 2253 3749 3782 3887 4019 4307 4733
Size: 1893 360 1496 33 105 132 288 426
Cuts at: 4733 5261 5364 7496 7542 8135 8704 9038 12671
Size: 528 103 2132 46 593 569 334 3633
Cuts at: 12671 12875 13982 15498 15682 15900
Size: 204 1107 1516 184 218
Fragments arranged by size:
3633 2132 1893 1516 1496 1107 593 569
528 426 360 334 288 218 204 184
132 105 103 46 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 5449 8578 9668 12103 12129 13339 15900
Size: 436 5013 3129 1090 2435 26 1210 2561
Fragments arranged by size:
5013 3129 2561 2435 1210 1090 436 26
BsiEI CG_ry'CG
Cuts at: 0 91 1261 7507 8100 15006 15900
Size: 91 1170 6246 593 6906 894
Fragments arranged by size:
6906 6246 1170 894 593 91
BsiHKAI G_wGCw'C
Cuts at: 0 3287 4707 5960 6442 6590 6638 6658 7807
Size: 3287 1420 1253 482 148 48 20 1149
Cuts at: 7807 8009 8390 8913 9833 10580 10620 11111 12524
Size: 202 381 523 920 747 40 491 1413
Cuts at: 12524 13190 13587 13808 13978 14127 14307 14393 15306
Size: 666 397 221 170 149 180 86 913
Cuts at: 15306 15900
Size: 594
Fragments arranged by size:
3287 1420 1413 1253 1149 920 913 747
666 594 523 491 482 397 381 221
202 180 170 149 148 86 48 40
20
BsmI GAATG_Cn'
Cuts at: 0 4539 7581 15900
Size: 4539 3042 8319
Fragments arranged by size:
8319 4539 3042
BsmBI CGTCTCn'nnnn_
Cuts at: 0 13 12289 15745 15900
Size: 13 12276 3456 155
Fragments arranged by size:
12276 3456 155 13
Bsp1286I G_dGCh'C
Cuts at: 0 1900 3287 4707 4876 5960 6442 6590 6638
Size: 1900 1387 1420 169 1084 482 148 48
Cuts at: 6638 6658 7550 7807 8009 8390 8913 9833 10580
Size: 20 892 257 202 381 523 920 747
Cuts at: 10580 10620 10637 11070 11111 11139 11376 12399 12524
Size: 40 17 433 41 28 237 1023 125
Cuts at: 12524 13190 13587 13808 13978 14127 14307 14393 15306
Size: 666 397 221 170 149 180 86 913
Cuts at: 15306 15900
Size: 594
Fragments arranged by size:
1900 1420 1387 1084 1023 920 913 892
747 666 594 523 482 433 397 381
257 237 221 202 180 170 169 149
148 125 86 48 41 40 28 20
17
BspEI T'CCGG_A
Cuts at: 0 2021 6794 15900
Size: 2021 4773 9106
Fragments arranged by size:
9106 4773 2021
BspHI T'CATG_A
Cuts at: 0 7095 11172 11424 13826 14180 15900
Size: 7095 4077 252 2402 354 1720
Fragments arranged by size:
7095 4077 2402 1720 354 252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 1452 1841 2486 4392 5415 6204 9603
Size: 146 1306 389 645 1906 1023 789 3399
Cuts at: 9603 14718 15900
Size: 5115 1182
Fragments arranged by size:
5115 3399 1906 1306 1182 1023 789 645
389 146
BsrBI CCG'CTC
Cuts at: 0 33 51 87 8232 8618 11064 13689 14751
Size: 33 18 36 8145 386 2446 2625 1062
Cuts at: 14751 15383 15900
Size: 632 517
Fragments arranged by size:
8145 2625 2446 1062 632 517 386 36
33 18
BsrDI GCAATG_nn'
Cuts at: 0 477 1833 2744 3776 5227 6281 9799 12603
Size: 477 1356 911 1032 1451 1054 3518 2804
Cuts at: 12603 15900
Size: 3297
Fragments arranged by size:
3518 3297 2804 1451 1356 1054 1032 911
477
BsrFI r'CCGG_y
Cuts at: 0 1260 1395 14021 15182 15713 15900
Size: 1260 135 12626 1161 531 187
Fragments arranged by size:
12626 1260 1161 531 187 135
BsrGI T'GTAC_A
Cuts at: 0 1424 1567 2907 3143 5758 9666 15469 15900
Size: 1424 143 1340 236 2615 3908 5803 431
Fragments arranged by size:
5803 3908 2615 1424 1340 431 236 143
BssSI C'ACGA_G
Cuts at: 0 2384 5993 7963 15305 15900
Size: 2384 3609 1970 7342 595
Fragments arranged by size:
7342 3609 2384 1970 595
BstAPI GCAn_nnn'nTGC
Cuts at: 0 199 414 2847 5349 8125 15900
Size: 199 215 2433 2502 2776 7775
Fragments arranged by size:
7775 2776 2502 2433 215 199
BstBI TT'CG_AA
Cuts at: 0 6404 15900
Size: 6404 9496
BstEII G'GTnAC_C
Cuts at: 0 1548 6324 14553 15900
Size: 1548 4776 8229 1347
Fragments arranged by size:
8229 4776 1548 1347
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2793 3436 3678 15900
Size: 2793 643 242 12222
Fragments arranged by size:
12222 2793 643 242
BstYI r'GATC_y
Cuts at: 0 312 542 1467 1475 3564 4466 4671 4740
Size: 312 230 925 8 2089 902 205 69
Cuts at: 4740 5770 6578 7026 7037 8550 8852 8998 9588
Size: 1030 808 448 11 1513 302 146 590
Cuts at: 9588 9884 11637 11930 14414 15900
Size: 296 1753 293 2484 1486
Fragments arranged by size:
2484 2089 1753 1513 1486 1030 925 902
808 590 448 312 302 296 293 230
205 146 69 11 8
Bsu36I CC'TnA_GG
Cuts at: 0 902 5336 8942 11291 15900
Size: 902 4434 3606 2349 4609
Fragments arranged by size:
4609 4434 3606 2349 902
BtgI C'CryG_G
Cuts at: 0 2492 3104 3849 7205 7507 8500 11549 11595
Size: 2492 612 745 3356 302 993 3049 46
Cuts at: 11595 14228 14695 15900
Size: 2633 467 1205
Fragments arranged by size:
3356 3049 2633 2492 1205 993 745 612
467 302 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 8006 15900
Size: 8006 7894
BtsI GCAGTG_nn'
Cuts at: 0 142 1357 2311 3881 4985 5738 6371 7177
Size: 142 1215 954 1570 1104 753 633 806
Cuts at: 7177 9123 15866 15900
Size: 1946 6743 34
Fragments arranged by size:
6743 1946 1570 1215 1104 954 806 753
633 142 34
ClaI AT'CG_AT
Cuts at: 0 5054 14936 15900
Size: 5054 9882 964
Fragments arranged by size:
9882 5054 964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4797 4832 4865 4900 9357 9392 14245 14280
Size: 4797 35 33 35 4457 35 4853 35
Cuts at: 14280 15104 15139 15900
Size: 824 35 761
Fragments arranged by size:
4853 4797 4457 824 761 35 35 35
35 35 33
DraI TTT'AAA
Cuts at: 0 9564 10232 13130 15900
Size: 9564 668 2898 2770
Fragments arranged by size:
9564 2898 2770 668
DraIII CAC_nnn'GTG
Cuts at: 0 257 15900
Size: 257 15643
DrdI GACnn_nn'nnGTC
Cuts at: 0 12505 14821 15900
Size: 12505 2316 1079
Fragments arranged by size:
12505 2316 1079
EaeI y'GGCC_r
Cuts at: 0 324 1094 1161 1258 1724 2795 3134 4475
Size: 324 770 67 97 466 1071 339 1341
Cuts at: 4475 5282 5319 7504 9114 9627 10097 10424 15900
Size: 807 37 2185 1610 513 470 327 5476
Fragments arranged by size:
5476 2185 1610 1341 1071 807 770 513
470 466 339 327 324 97 67 37
EagI C'GGCC_G
Cuts at: 0 1258 7504 15900
Size: 1258 6246 8396
Fragments arranged by size:
8396 6246 1258
EarI CTCTTCn'nnn_
Cuts at: 0 11 1202 1502 1784 1883 3050 4134 4256
Size: 11 1191 300 282 99 1167 1084 122
Cuts at: 4256 4409 4847 4898 4937 6901 7916 8255 8348
Size: 153 438 51 39 1964 1015 339 93
Cuts at: 8348 8399 8732 9311 9599 9686 10298 10933 11099
Size: 51 333 579 288 87 612 635 166
Cuts at: 11099 11885 11906 11983 12104 12458 12887 13043 13178
Size: 786 21 77 121 354 429 156 135
Cuts at: 13178 15056 15900
Size: 1878 844
Fragments arranged by size:
1964 1878 1191 1167 1084 1015 844 786
635 612 579 438 429 354 339 333
300 288 282 166 156 153 135 122
121 99 93 87 77 51 51 39
21 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4977 15900
Size: 4977 10923
EcoICRI GAG'CTC
Cuts at: 0 6440 6588 6656 8388 8911 9831 11109 12522
Size: 6440 148 68 1732 523 920 1278 1413
Cuts at: 12522 14305 14391 15900
Size: 1783 86 1509
Fragments arranged by size:
6440 1783 1732 1509 1413 1278 920 523
148 86 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 824 924 1456 1519 1760 1828 2002
Size: 687 137 100 532 63 241 68 174
Cuts at: 2002 2020 2142 2206 2358 2413 2467 2962 3199
Size: 18 122 64 152 55 54 495 237
Cuts at: 3199 3598 4115 4258 4466 4570 4579 4687 4921
Size: 399 517 143 208 104 9 108 234
Cuts at: 4921 4990 5063 5422 5440 5533 5893 5921 6181
Size: 69 73 359 18 93 360 28 260
Cuts at: 6181 6857 6925 7099 7700 7705 7810 8059 8098
Size: 676 68 174 601 5 105 249 39
Cuts at: 8098 8336 8369 8651 8917 9124 9199 9308 9493
Size: 238 33 282 266 207 75 109 185
Cuts at: 9493 9580 9889 10036 10659 10852 10914 11144 11476
Size: 87 309 147 623 193 62 230 332
Cuts at: 11476 11522 11732 11938 11999 12085 12641 12700 13486
Size: 46 210 206 61 86 556 59 786
Cuts at: 13486 13918 13948 14114 14702 14767 14806 14847 15426
Size: 432 30 166 588 65 39 41 579
Cuts at: 15426 15473 15761 15795 15869 15900
Size: 47 288 34 74 31
Fragments arranged by size:
786 687 676 623 601 588 579 556
532 517 495 432 399 360 359 332
309 288 282 266 260 249 241 238
237 234 230 210 208 207 206 193
185 174 174 166 152 147 143 137
122 109 108 105 104 100 93 87
86 75 74 73 69 68 68 65
64 63 62 61 59 55 54 47
46 41 39 39 34 33 31 30
28 18 18 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2482 3652 4543 8072 10843 11294 15349 15876
Size: 2482 1170 891 3529 2771 451 4055 527
Cuts at: 15876 15900
Size: 24
Fragments arranged by size:
4055 3529 2771 2482 1170 891 527 451
24
EcoO109I rG'GnC_Cy
Cuts at: 0 351 6809 7546 7547 7675 8168 8931 9504
Size: 351 6458 737 1 128 493 763 573
Cuts at: 9504 10392 13053 13812 13893 14145 14319 15889 15900
Size: 888 2661 759 81 252 174 1570 11
Fragments arranged by size:
6458 2661 1570 888 763 759 737 573
493 351 252 174 128 81 11 1
EcoRI G'AATT_C
Cuts at: 0 3209 3630 6790 7293 13072 13080 14176 15900
Size: 3209 421 3160 503 5779 8 1096 1724
Fragments arranged by size:
5779 3209 3160 1724 1096 503 421 8
EcoRV GAT'ATC
Cuts at: 0 13475 15900
Size: 13475 2425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 6318 15746 15900
Size: 6318 9428 154
Fragments arranged by size:
9428 6318 154
HaeII r_GCGC'y
Cuts at: 0 3732 4113 4936 8616 11595 15791 15900
Size: 3732 381 823 3680 2979 4196 109
Fragments arranged by size:
4196 3732 3680 2979 823 381 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 495 527 782 814 820 835 852 867
Size: 495 32 255 32 6 15 17 15
Cuts at: 867 902 934 2380 2412 3681 3713 5043 5075
Size: 35 32 1446 32 1269 32 1330 32
Cuts at: 5075 5627 5659 6751 6783 7201 7233 7716 7748
Size: 552 32 1092 32 418 32 483 32
Cuts at: 7748 7984 8016 8419 8451 9132 9164 9687 9719
Size: 236 32 403 32 681 32 523 32
Cuts at: 9719 9986 10018 10126 10158 10845 10877 10986 11018
Size: 267 32 108 32 687 32 109 32
Cuts at: 11018 11510 11542 11886 11918 12888 12920 12940 12972
Size: 492 32 344 32 970 32 20 32
Cuts at: 12972 13214 13246 14051 14083 14200 14225 14232 14257
Size: 242 32 805 32 117 25 7 25
Cuts at: 14257 14907 14939 15343 15375 15742 15774 15873 15900
Size: 650 32 404 32 367 32 99 27
Fragments arranged by size:
1446 1330 1269 1092 970 805 687 681
650 552 523 495 492 483 418 404
403 367 344 267 255 242 236 117
109 108 99 35 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 27
25 25 20 17 15 15 7 6
HincII GTy'rAC
Cuts at: 0 264 503 2174 2856 3593 4886 9301 12585
Size: 264 239 1671 682 737 1293 4415 3284
Cuts at: 12585 12722 13758 14214 15900
Size: 137 1036 456 1686
Fragments arranged by size:
4415 3284 1686 1671 1293 1036 737 682
456 264 239 137
HindIII A'AGCT_T
Cuts at: 0 2929 3068 4903 5577 6451 8802 9758 10159
Size: 2929 139 1835 674 874 2351 956 401
Cuts at: 10159 12250 13409 15900
Size: 2091 1159 2491
Fragments arranged by size:
2929 2491 2351 2091 1835 1159 956 874
674 401 139
HpaI GTT'AAC
Cuts at: 0 2856 14214 15900
Size: 2856 11358 1686
Fragments arranged by size:
11358 2856 1686
MfeI C'AATT_G
Cuts at: 0 3307 4528 6128 6957 7915 9151 15900
Size: 3307 1221 1600 829 958 1236 6749
Fragments arranged by size:
6749 3307 1600 1236 1221 958 829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 40 326 5471 6502 6597 6764 7249
Size: 22 18 286 5145 1031 95 167 485
Cuts at: 7249 7265 7836 8156 10571 11426 13682 14060 14827
Size: 16 571 320 2415 855 2256 378 767
Cuts at: 14827 15553 15794 15900
Size: 726 241 106
Fragments arranged by size:
5145 2415 2256 1031 855 767 726 571
485 378 320 286 241 167 106 95
22 18 16
MscI TGG'CCA
Cuts at: 0 326 1096 1726 2797 3136 4477 5284 5321
Size: 326 770 630 1071 339 1341 807 37
Cuts at: 5321 9116 9629 10099 10426 15900
Size: 3795 513 470 327 5474
Fragments arranged by size:
5474 3795 1341 1071 807 770 630 513
470 339 327 326 37
MslI CAynn'nnrTG
Cuts at: 0 296 865 1193 1231 1880 2791 3434 5188
Size: 296 569 328 38 649 911 643 1754
Cuts at: 5188 6038 6262 10940 12263 15235 15900
Size: 850 224 4678 1323 2972 665
Fragments arranged by size:
4678 2972 1754 1323 911 850 665 649
643 569 328 296 224 38
MspA1I CmG'CkG
Cuts at: 0 625 1458 2071 2319 2697 2847 3753 5103
Size: 625 833 613 248 378 150 906 1350
Cuts at: 5103 6399 9417 9809 9909 10014 10077 10120 10422
Size: 1296 3018 392 100 105 63 43 302
Cuts at: 10422 12879 13785 14445 14621 14720 15467 15900
Size: 2457 906 660 176 99 747 433
Fragments arranged by size:
3018 2457 1350 1296 906 906 833 747
660 625 613 433 392 378 302 248
176 150 105 100 99 63 43
NaeI GCC'GGC
Cuts at: 0 1262 14023 15715 15900
Size: 1262 12761 1692 185
Fragments arranged by size:
12761 1692 1262 185
NcoI C'CATG_G
Cuts at: 0 2492 3104 3849 7205 8500 14228 15900
Size: 2492 612 745 3356 1295 5728 1672
Fragments arranged by size:
5728 3356 2492 1672 1295 745 612
NdeI CA'TA_TG
Cuts at: 0 2115 9305 12860 15900
Size: 2115 7190 3555 3040
Fragments arranged by size:
7190 3555 3040 2115
NgoMIV G'CCGG_C
Cuts at: 0 1260 14021 15713 15900
Size: 1260 12761 1692 187
Fragments arranged by size:
12761 1692 1260 187
NheI G'CTAG_C
Cuts at: 0 5143 15900
Size: 5143 10757
NsiI A_TGCA'T
Cuts at: 0 3264 5525 6765 15900
Size: 3264 2261 1240 9135
Fragments arranged by size:
9135 3264 2261 1240
NspI r_CATG'y
Cuts at: 0 2344 2854 4122 4282 5523 6043 7053 7572
Size: 2344 510 1268 160 1241 520 1010 519
Cuts at: 7572 8485 9945 10961 11340 12427 13009 14076 15900
Size: 913 1460 1016 379 1087 582 1067 1824
Fragments arranged by size:
2344 1824 1460 1268 1241 1087 1067 1016
1010 913 582 520 519 510 379 160
PasI CC'CwG_GG
Cuts at: 0 115 507 12738 15363 15670 15900
Size: 115 392 12231 2625 307 230
Fragments arranged by size:
12231 2625 392 307 230 115
PciI A'CATG_T
Cuts at: 0 4278 7049 7568 10957 11336 15900
Size: 4278 2771 519 3389 379 4564
Fragments arranged by size:
4564 4278 3389 2771 519 379
PflMI CCAn_nnn'nTGG
Cuts at: 0 4665 8721 11823 12493 15505 15900
Size: 4665 4056 3102 670 3012 395
Fragments arranged by size:
4665 4056 3102 3012 670 395
PfoI T'CCnGG_A
Cuts at: 0 2483 3647 3818 4937 5063 5118 5909 6149
Size: 2483 1164 171 1119 126 55 791 240
Cuts at: 6149 6242 6581 7826 8346 8690 8795 10331 11657
Size: 93 339 1245 520 344 105 1536 1326
Cuts at: 11657 13964 14393 15811 15900
Size: 2307 429 1418 89
Fragments arranged by size:
2483 2307 1536 1418 1326 1245 1164 1119
791 520 429 344 339 240 171 126
105 93 89 55
PmlI CAC'GTG
Cuts at: 0 4206 4879 4951 15900
Size: 4206 673 72 10949
Fragments arranged by size:
10949 4206 673 72
PpuMI rG'GwC_Cy
Cuts at: 0 351 6809 8168 13812 13893 14319 15889 15900
Size: 351 6458 1359 5644 81 426 1570 11
Fragments arranged by size:
6458 5644 1570 1359 426 351 81 11
PsiI TTA'TAA
Cuts at: 0 1058 12902 15900
Size: 1058 11844 2998
Fragments arranged by size:
11844 2998 1058
PspOMI G'GGCC_C
Cuts at: 0 7546 11066 15900
Size: 7546 3520 4834
Fragments arranged by size:
7546 4834 3520
PstI C_TGCA'G
Cuts at: 0 195 410 1364 1463 3005 3758 4637 5465
Size: 195 215 954 99 1542 753 879 828
Cuts at: 5465 5834 6122 6374 6500 7172 7491 8427 8747
Size: 369 288 252 126 672 319 936 320
Cuts at: 8747 8930 11570 12731 14153 15795 15900
Size: 183 2640 1161 1422 1642 105
Fragments arranged by size:
2640 1642 1542 1422 1161 954 936 879
828 753 672 369 320 319 288 252
215 195 183 126 105 99
PvuI CG_AT'CG
Cuts at: 0 8100 15900
Size: 8100 7800
PvuII CAG'CTG
Cuts at: 0 625 1458 2071 2319 2847 3753 5103 9417
Size: 625 833 613 248 528 906 1350 4314
Cuts at: 9417 9809 10014 10077 10120 10422 12879 13785 15467
Size: 392 205 63 43 302 2457 906 1682
Cuts at: 15467 15900
Size: 433
Fragments arranged by size:
4314 2457 1682 1350 906 906 833 625
613 528 433 392 302 248 205 63
43
SacI G_AGCT'C
Cuts at: 0 6442 6590 6658 8390 8913 9833 11111 12524
Size: 6442 148 68 1732 523 920 1278 1413
Cuts at: 12524 14307 14393 15900
Size: 1783 86 1507
Fragments arranged by size:
6442 1783 1732 1507 1413 1278 920 523
148 86 68
SalI G'TCGA_C
Cuts at: 0 3591 9299 15900
Size: 3591 5708 6601
Fragments arranged by size:
6601 5708 3591
SapI GCTCTTCn'nnn_
Cuts at: 0 1502 1883 4409 4898 8399 8732 10298 11099
Size: 1502 381 2526 489 3501 333 1566 801
Cuts at: 11099 11906 11983 13178 15056 15900
Size: 807 77 1195 1878 844
Fragments arranged by size:
3501 2526 1878 1566 1502 1195 844 807
801 489 381 333 77
SbfI CC_TGCA'GG
Cuts at: 0 5834 15900
Size: 5834 10066
ScaI AGT'ACT
Cuts at: 0 3557 4027 6354 7091 15900
Size: 3557 470 2327 737 8809
Fragments arranged by size:
8809 3557 2327 737 470
SexAI A'CCwGG_T
Cuts at: 0 6638 8170 15900
Size: 6638 1532 7730
Fragments arranged by size:
7730 6638 1532
SfcI C'TryA_G
Cuts at: 0 191 406 1288 1360 1459 2066 2634 3001
Size: 191 215 882 72 99 607 568 367
Cuts at: 3001 3754 4633 5461 5830 6053 6090 6118 6356
Size: 753 879 828 369 223 37 28 238
Cuts at: 6356 6370 6418 6496 6730 7168 7487 8236 8248
Size: 14 48 78 234 438 319 749 12
Cuts at: 8248 8423 8743 8880 8926 9450 9751 11566 12727
Size: 175 320 137 46 524 301 1815 1161
Cuts at: 12727 14149 15791 15900
Size: 1422 1642 109
Fragments arranged by size:
1815 1642 1422 1161 882 879 828 753
749 607 568 524 438 369 367 320
319 301 238 234 223 215 191 175
137 109 99 78 72 48 46 37
28 14 12
SmaI CCC'GGG
Cuts at: 0 376 15415 15900
Size: 376 15039 485
Fragments arranged by size:
15039 485 376
SmlI C'TyrA_G
Cuts at: 0 1178 1899 2242 2403 3787 4145 4161 5613
Size: 1178 721 343 161 1384 358 16 1452
Cuts at: 5613 6631 8026 8443 8848 8873 12058 12639 14026
Size: 1018 1395 417 405 25 3185 581 1387
Cuts at: 14026 14867 15900
Size: 841 1033
Fragments arranged by size:
3185 1452 1395 1387 1384 1178 1033 1018
841 721 581 417 405 358 343 161
25 16
SnaBI TAC'GTA
Cuts at: 0 3490 15900
Size: 3490 12410
SpeI A'CTAG_T
Cuts at: 0 456 10695 15900
Size: 456 10239 5205
Fragments arranged by size:
10239 5205 456
SphI G_CATG'C
Cuts at: 0 4122 12427 15900
Size: 4122 8305 3473
Fragments arranged by size:
8305 4122 3473
SspI AAT'ATT
Cuts at: 0 465 2437 8633 8814 15900
Size: 465 1972 6196 181 7086
Fragments arranged by size:
7086 6196 1972 465 181
StuI AGG'CCT
Cuts at: 0 4075 5302 5836 7270 9283 11584 14035 15900
Size: 4075 1227 534 1434 2013 2301 2451 1865
Fragments arranged by size:
4075 2451 2301 2013 1865 1434 1227 534
StyI C'CwwG_G
Cuts at: 0 587 1097 1661 2492 3104 3665 3671 3849
Size: 587 510 564 831 612 561 6 178
Cuts at: 3849 4719 5162 7014 7205 8073 8500 9791 10388
Size: 870 443 1852 191 868 427 1291 597
Cuts at: 10388 11934 12408 12887 13638 14228 14774 15266 15512
Size: 1546 474 479 751 590 546 492 246
Cuts at: 15512 15801 15900
Size: 289 99
Fragments arranged by size:
1852 1546 1291 870 868 831 751 612
597 590 587 564 561 546 510 492
479 474 443 427 289 246 191 178
99 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2267 2787 3304 4299 4747 5302 7822 7906
Size: 2267 520 517 995 448 555 2520 84
Cuts at: 7906 8149 9175 9541 11818 12346 14182 14492 15020
Size: 243 1026 366 2277 528 1836 310 528
Cuts at: 15020 15900
Size: 880
Fragments arranged by size:
2520 2277 2267 1836 1026 995 880 555
528 528 520 517 448 366 310 243
84
TatI w'GTAC_w
Cuts at: 0 1424 1567 2907 3143 3555 4025 5758 6352
Size: 1424 143 1340 236 412 470 1733 594
Cuts at: 6352 7089 7707 9666 10688 15469 15900
Size: 737 618 1959 1022 4781 431
Fragments arranged by size:
4781 1959 1733 1424 1340 1022 737 618
594 470 431 412 236 143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 928 960 1195 1227 6554 6586 6929 6961
Size: 928 32 235 32 5327 32 343 32
Cuts at: 6961 7027 7059 7325 7357 8278 8310 8535 8567
Size: 66 32 266 32 921 32 225 32
Cuts at: 8567 10706 10738 11477 11509 12985 13017 15900
Size: 2139 32 739 32 1476 32 2883
Fragments arranged by size:
5327 2883 2139 1476 928 921 739 343
266 235 225 66 32 32 32 32
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3430 10998 13822 13990 14065 14551 15756 15900
Size: 3430 7568 2824 168 75 486 1205 144
Fragments arranged by size:
7568 3430 2824 1205 486 168 144 75
XbaI T'CTAG_A
Cuts at: 0 1560 15900
Size: 1560 14340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 2265 6318 9806 10653 15900
Size: 504 1761 4053 3488 847 5247
Fragments arranged by size:
5247 4053 3488 1761 847 504
XhoI C'TCGA_G
Cuts at: 0 3787 14867 15900
Size: 3787 11080 1033
Fragments arranged by size:
11080 3787 1033
XmaI C'CCGG_G
Cuts at: 0 374 15413 15900
Size: 374 15039 487
Fragments arranged by size:
15039 487 374
XmnI GAAnn'nnTTC
Cuts at: 0 247 682 1610 2436 2930 2996 4904 7112
Size: 247 435 928 826 494 66 1908 2208
Cuts at: 7112 8264 10882 12208 14054 14402 15900
Size: 1152 2618 1326 1846 348 1498
Fragments arranged by size:
2618 2208 1908 1846 1498 1326 1152 928
826 494 435 348 247 66
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AloI AscI AsiSI BbeI BsiWI BssHII
BstZ17I FseI FspI FspAI KasI KpnI MluI NarI
NotI NruI PacI PmeI PpiI PshAI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SgfI SgrAI SrfI
SwaI ZraI