Restriction map for
pFN21AE0599
(Linear) (Six-base) MAPSORT of: KIEE0599.seq Check: 9843 from: 1 to: 576
KIEE0599.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AatII G_ACGT'C
Cuts at: 0 530 576
Size: 530 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 576
Size: 366 210
AlwNI CAG_nnn'CTG
Cuts at: 0 217 292 311 576
Size: 217 75 19 265
Fragments arranged by size:
265 217 75 19
ApaLI G'TGCA_C
Cuts at: 0 265 576
Size: 265 311
ApoI r'AATT_y
Cuts at: 0 11 576
Size: 11 565
BamHI G'GATC_C
Cuts at: 0 252 576
Size: 252 324
BanI G'GyrC_C
Cuts at: 0 112 192 576
Size: 112 80 384
Fragments arranged by size:
384 112 80
BbeI G_GCGC'C
Cuts at: 0 116 576
Size: 116 460
BglI GCCn_nnn'nGGC
Cuts at: 0 281 576
Size: 281 295
Bme1580I G_kGCm'C
Cuts at: 0 197 269 576
Size: 197 72 307
Fragments arranged by size:
307 197 72
BsaHI Gr'CG_yC
Cuts at: 0 113 527 576
Size: 113 414 49
Fragments arranged by size:
414 113 49
BsaWI w'CCGG_w
Cuts at: 0 50 576
Size: 50 526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 21 51 576
Size: 21 30 525
Fragments arranged by size:
525 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 386 576
Size: 386 190
BsiHKAI G_wGCw'C
Cuts at: 0 269 576
Size: 269 307
Bsp1286I G_dGCh'C
Cuts at: 0 197 269 576
Size: 197 72 307
Fragments arranged by size:
307 197 72
BspEI T'CCGG_A
Cuts at: 0 50 576
Size: 50 526
BsrDI GCAATG_nn'
Cuts at: 0 349 576
Size: 349 227
BsrFI r'CCGG_y
Cuts at: 0 69 535 576
Size: 69 466 41
Fragments arranged by size:
466 69 41
BssSI C'ACGA_G
Cuts at: 0 561 576
Size: 561 15
BstYI r'GATC_y
Cuts at: 0 252 576
Size: 252 324
Bsu36I CC'TnA_GG
Cuts at: 0 550 576
Size: 550 26
BtgI C'CryG_G
Cuts at: 0 276 355 576
Size: 276 79 221
Fragments arranged by size:
276 221 79
EaeI y'GGCC_r
Cuts at: 0 23 71 273 576
Size: 23 48 202 303
Fragments arranged by size:
303 202 48 23
EarI CTCTTCn'nnn_
Cuts at: 0 58 488 576
Size: 58 430 88
Fragments arranged by size:
430 88 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 576
Size: 366 210
EcoNI CCTnn'n_nnAGG
Cuts at: 0 553 576
Size: 553 23
EcoO109I rG'GnC_Cy
Cuts at: 0 214 576
Size: 214 362
FseI GG_CCGG'CC
Cuts at: 0 73 576
Size: 73 503
FspI TGC'GCA
Cuts at: 0 152 576
Size: 152 424
HaeII r_GCGC'y
Cuts at: 0 116 576
Size: 116 460
KasI G'GCGC_C
Cuts at: 0 112 576
Size: 112 464
MfeI C'AATT_G
Cuts at: 0 17 576
Size: 17 559
MscI TGG'CCA
Cuts at: 0 25 275 576
Size: 25 250 301
Fragments arranged by size:
301 250 25
MslI CAynn'nnrTG
Cuts at: 0 354 576
Size: 354 222
MspA1I CmG'CkG
Cuts at: 0 98 576
Size: 98 478
NaeI GCC'GGC
Cuts at: 0 71 576
Size: 71 505
NarI GG'CG_CC
Cuts at: 0 113 576
Size: 113 463
NgoMIV G'CCGG_C
Cuts at: 0 69 576
Size: 69 507
PfoI T'CCnGG_A
Cuts at: 0 39 576
Size: 39 537
PpuMI rG'GwC_Cy
Cuts at: 0 214 576
Size: 214 362
SfoI GGC'GCC
Cuts at: 0 114 576
Size: 114 462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 21 53 576
Size: 21 32 523
Fragments arranged by size:
523 32 21
Tth111I GACn'n_nGTC
Cuts at: 0 529 576
Size: 529 47
ZraI GAC'GTC
Cuts at: 0 528 576
Size: 528 48
Enzymes that do cut:
AatII AcuI AlwNI ApaLI ApoI BamHI BanI BbeI
BglI Bme1580I BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I
BspEI BsrDI BsrFI BssSI BstYI Bsu36I BtgI EaeI
EarI Eco57MI EcoNI EcoO109I FseI FspI HaeII KasI
MfeI MscI MslI MspA1I NaeI NarI NgoMIV PfoI
PpuMI SfoI TstI Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FspAI Hin4I HincII HindIII HpaI
KpnI MluI MmeI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI XbaI XcmI XhoI XmaI XmnI