Restriction map for
pFN21AE0590
(Linear) (Six-base) MAPSORT of: KIEE0590.seq Check: 773 from: 1 to: 1305
KIEE0590.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 723 1305
Size: 492 231 582
Fragments arranged by size:
582 492 231
AflIII A'CryG_T
Cuts at: 0 1196 1305
Size: 1196 109
AhdI GACnn_n'nnGTC
Cuts at: 0 1017 1305
Size: 1017 288
AleI CACnn'nnGTG
Cuts at: 0 609 1305
Size: 609 696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 1305
Size: 112 1193
AlwNI CAG_nnn'CTG
Cuts at: 0 555 1305
Size: 555 750
ApaI G_GGCC'C
Cuts at: 0 530 979 1305
Size: 530 449 326
Fragments arranged by size:
530 449 326
ApaLI G'TGCA_C
Cuts at: 0 67 1305
Size: 67 1238
ApoI r'AATT_y
Cuts at: 0 383 1305
Size: 383 922
AvaI C'yCGr_G
Cuts at: 0 238 635 1205 1305
Size: 238 397 570 100
Fragments arranged by size:
570 397 238 100
BanI G'GyrC_C
Cuts at: 0 79 173 251 920 1095 1305
Size: 79 94 78 669 175 210
Fragments arranged by size:
669 210 175 94 79 78
BanII G_rGCy'C
Cuts at: 0 239 530 944 979 1301 1305
Size: 239 291 414 35 322 4
Fragments arranged by size:
414 322 291 239 35 4
BbeI G_GCGC'C
Cuts at: 0 83 177 255 1305
Size: 83 94 78 1050
Fragments arranged by size:
1050 94 83 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 1305
Size: 258 1047
BbvCI CC'TCA_GC
Cuts at: 0 124 1305
Size: 124 1181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 695 729 1305
Size: 695 34 576
Fragments arranged by size:
695 576 34
BclI T'GATC_A
Cuts at: 0 860 1305
Size: 860 445
BglI GCCn_nnn'nGGC
Cuts at: 0 157 226 249 1305
Size: 157 69 23 1056
Fragments arranged by size:
1056 157 69 23
BlpI GC'TnA_GC
Cuts at: 0 145 1305
Size: 145 1160
Bme1580I G_kGCm'C
Cuts at: 0 71 530 979 1305
Size: 71 459 449 326
Fragments arranged by size:
459 449 326 71
BmgBI CAC'GTC
Cuts at: 0 66 1227 1305
Size: 66 1161 78
Fragments arranged by size:
1161 78 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 434 1305
Size: 434 871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 277 502 577 592 958 1305
Size: 34 243 225 75 15 366 347
Fragments arranged by size:
366 347 243 225 75 34 15
Bpu10I CC'TnA_GC
Cuts at: 0 124 1305
Size: 124 1181
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 584 1305
Size: 109 475 721
Fragments arranged by size:
721 475 109
BsaAI yAC'GTr
Cuts at: 0 585 1218 1305
Size: 585 633 87
Fragments arranged by size:
633 585 87
BsaHI Gr'CG_yC
Cuts at: 0 80 174 252 416 590 1305
Size: 80 94 78 164 174 715
Fragments arranged by size:
715 174 164 94 80 78
BsaWI w'CCGG_w
Cuts at: 0 656 1293 1305
Size: 656 637 12
Fragments arranged by size:
656 637 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 70 733 1102 1105 1246 1305
Size: 31 39 663 369 3 141 59
Fragments arranged by size:
663 369 141 59 39 31 3
BseYI C'CCAG_C
Cuts at: 0 75 99 1305
Size: 75 24 1206
Fragments arranged by size:
1206 75 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 725 1305
Size: 459 266 580
Fragments arranged by size:
580 459 266
BsiEI CG_ry'CG
Cuts at: 0 197 790 1305
Size: 197 593 515
Fragments arranged by size:
593 515 197
BsiHKAI G_wGCw'C
Cuts at: 0 71 944 1305
Size: 71 873 361
Fragments arranged by size:
873 361 71
Bsp1286I G_dGCh'C
Cuts at: 0 71 239 530 944 979 1301 1305
Size: 71 168 291 414 35 322 4
Fragments arranged by size:
414 322 291 168 71 35 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1169 1305
Size: 1169 136
BsrBI CCG'CTC
Cuts at: 0 214 1305
Size: 214 1091
BsrDI GCAATG_nn'
Cuts at: 0 518 1064 1305
Size: 518 546 241
Fragments arranged by size:
546 518 241
BsrFI r'CCGG_y
Cuts at: 0 800 1305
Size: 800 505
BssHII G'CGCG_C
Cuts at: 0 137 1305
Size: 137 1168
Bsu36I CC'TnA_GG
Cuts at: 0 1166 1305
Size: 1166 139
BtgI C'CryG_G
Cuts at: 0 84 244 322 1305
Size: 84 160 78 983
Fragments arranged by size:
983 160 84 78
BtsI GCAGTG_nn'
Cuts at: 0 301 1305
Size: 301 1004
EaeI y'GGCC_r
Cuts at: 0 158 194 1028 1305
Size: 158 36 834 277
Fragments arranged by size:
834 277 158 36
EagI C'GGCC_G
Cuts at: 0 194 1305
Size: 194 1111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 247 605 1305
Size: 247 358 700
Fragments arranged by size:
700 358 247
EcoICRI GAG'CTC
Cuts at: 0 942 1305
Size: 942 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 277 492 502 577 592 723 958
Size: 34 243 215 10 75 15 131 235
Cuts at: 958 1305
Size: 347
Fragments arranged by size:
347 243 235 215 131 75 34 15
10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1165 1305
Size: 1165 140
EcoO109I rG'GnC_Cy
Cuts at: 0 526 527 639 975 976 1305
Size: 526 1 112 336 1 329
Fragments arranged by size:
526 336 329 112 1 1
HaeII r_GCGC'y
Cuts at: 0 83 131 152 177 255 334 1305
Size: 83 48 21 25 78 79 971
Fragments arranged by size:
971 83 79 78 48 25 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 1003 1035 1305
Size: 55 32 916 32 270
Fragments arranged by size:
916 270 55 32 32
KasI G'GCGC_C
Cuts at: 0 79 173 251 1305
Size: 79 94 78 1054
Fragments arranged by size:
1054 94 79 78
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 695 971 977 1305
Size: 413 282 276 6 328
Fragments arranged by size:
413 328 282 276 6
MslI CAynn'nnrTG
Cuts at: 0 609 1305
Size: 609 696
MspA1I CmG'CkG
Cuts at: 0 86 246 291 1038 1305
Size: 86 160 45 747 267
Fragments arranged by size:
747 267 160 86 45
NarI GG'CG_CC
Cuts at: 0 80 174 252 1305
Size: 80 94 78 1053
Fragments arranged by size:
1053 94 80 78
NspI r_CATG'y
Cuts at: 0 321 1200 1305
Size: 321 879 105
Fragments arranged by size:
879 321 105
PasI CC'CwG_GG
Cuts at: 0 491 1305
Size: 491 814
PciI A'CATG_T
Cuts at: 0 1196 1305
Size: 1196 109
PfoI T'CCnGG_A
Cuts at: 0 11 1190 1305
Size: 11 1179 115
Fragments arranged by size:
1179 115 11
PmlI CAC'GTG
Cuts at: 0 585 1305
Size: 585 720
PpuMI rG'GwC_Cy
Cuts at: 0 639 1305
Size: 639 666
PspOMI G'GGCC_C
Cuts at: 0 526 975 1305
Size: 526 449 330
Fragments arranged by size:
526 449 330
PstI C_TGCA'G
Cuts at: 0 121 1305
Size: 121 1184
PvuII CAG'CTG
Cuts at: 0 1038 1305
Size: 1038 267
SacI G_AGCT'C
Cuts at: 0 944 1305
Size: 944 361
SacII CC_GC'GG
Cuts at: 0 87 247 1305
Size: 87 160 1058
Fragments arranged by size:
1058 160 87
ScaI AGT'ACT
Cuts at: 0 736 1305
Size: 736 569
SfcI C'TryA_G
Cuts at: 0 117 1305
Size: 117 1188
SfoI GGC'GCC
Cuts at: 0 81 175 253 1305
Size: 81 94 78 1052
Fragments arranged by size:
1052 94 81 78
SmaI CCC'GGG
Cuts at: 0 637 1207 1305
Size: 637 570 98
Fragments arranged by size:
637 570 98
SrfI GCCC'GGGC
Cuts at: 0 1207 1305
Size: 1207 98
SspI AAT'ATT
Cuts at: 0 407 1305
Size: 407 898
StuI AGG'CCT
Cuts at: 0 1258 1305
Size: 1258 47
StyI C'CwwG_G
Cuts at: 0 816 1305
Size: 816 489
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 513 1305
Size: 513 792
TatI w'GTAC_w
Cuts at: 0 734 1073 1305
Size: 734 339 232
Fragments arranged by size:
734 339 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 392 424 1305
Size: 307 32 53 32 881
Fragments arranged by size:
881 307 53 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 933 1218 1305
Size: 933 285 87
Fragments arranged by size:
933 285 87
XmaI C'CCGG_G
Cuts at: 0 635 1205 1305
Size: 635 570 100
Fragments arranged by size:
635 570 100
XmnI GAAnn'nnTTC
Cuts at: 0 505 1270 1305
Size: 505 765 35
Fragments arranged by size:
765 505 35
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII Bsu36I
BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NarI
NspI PasI PciI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SacII ScaI SfcI SfoI SmaI SrfI
SspI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AloI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BfrBI BglII BmtI BplI BpuEI BsaBI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SwaI Tth111I
XbaI XhoI ZraI