Restriction map for
pFN21AE0466
(Linear) (Six-base) MAPSORT of: KIEE0466.seq Check: 3915 from: 1 to: 1005
KIEE0466.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 1005
Size: 147 858
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 542 898 1005
Size: 357 185 356 107
Fragments arranged by size:
357 356 185 107
AhdI GACnn_n'nnGTC
Cuts at: 0 304 1005
Size: 304 701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 226 1005
Size: 226 779
AlwNI CAG_nnn'CTG
Cuts at: 0 476 841 867 1005
Size: 476 365 26 138
Fragments arranged by size:
476 365 138 26
ApaLI G'TGCA_C
Cuts at: 0 231 1005
Size: 231 774
ApoI r'AATT_y
Cuts at: 0 600 702 1005
Size: 600 102 303
Fragments arranged by size:
600 303 102
AvaI C'yCGr_G
Cuts at: 0 67 1005
Size: 67 938
BanI G'GyrC_C
Cuts at: 0 119 140 163 210 1005
Size: 119 21 23 47 795
Fragments arranged by size:
795 119 47 23 21
BanII G_rGCy'C
Cuts at: 0 381 1005
Size: 381 624
BbeI G_GCGC'C
Cuts at: 0 123 144 1005
Size: 123 21 861
Fragments arranged by size:
861 123 21
BciVI GTATCCnnnnn_n'
Cuts at: 0 744 1005
Size: 744 261
BclI T'GATC_A
Cuts at: 0 594 638 1005
Size: 594 44 367
Fragments arranged by size:
594 367 44
BglI GCCn_nnn'nGGC
Cuts at: 0 63 97 138 1005
Size: 63 34 41 867
Fragments arranged by size:
867 63 41 34
Bme1580I G_kGCm'C
Cuts at: 0 166 235 1005
Size: 166 69 770
Fragments arranged by size:
770 166 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 863 1005
Size: 365 498 142
Fragments arranged by size:
498 365 142
BsaHI Gr'CG_yC
Cuts at: 0 120 141 1005
Size: 120 21 864
Fragments arranged by size:
864 120 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 6 760 1005
Size: 6 754 245
Fragments arranged by size:
754 245 6
BseYI C'CCAG_C
Cuts at: 0 42 1005
Size: 42 963
BsiHKAI G_wGCw'C
Cuts at: 0 235 338 1005
Size: 235 103 667
Fragments arranged by size:
667 235 103
Bsp1286I G_dGCh'C
Cuts at: 0 166 235 338 381 1005
Size: 166 69 103 43 624
Fragments arranged by size:
624 166 103 69 43
BspHI T'CATG_A
Cuts at: 0 641 1005
Size: 641 364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 269 1005
Size: 147 122 736
Fragments arranged by size:
736 147 122
BsrBI CCG'CTC
Cuts at: 0 633 1005
Size: 633 372
BsrDI GCAATG_nn'
Cuts at: 0 489 1005
Size: 489 516
BsrFI r'CCGG_y
Cuts at: 0 143 213 727 1005
Size: 143 70 514 278
Fragments arranged by size:
514 278 143 70
BsrGI T'GTAC_A
Cuts at: 0 917 1005
Size: 917 88
BssSI C'ACGA_G
Cuts at: 0 331 1005
Size: 331 674
BstEII G'GTnAC_C
Cuts at: 0 723 982 1005
Size: 723 259 23
Fragments arranged by size:
723 259 23
BstYI r'GATC_y
Cuts at: 0 182 941 1005
Size: 182 759 64
Fragments arranged by size:
759 182 64
BtgI C'CryG_G
Cuts at: 0 95 167 239 1005
Size: 95 72 72 766
Fragments arranged by size:
766 95 72 72
BtsI GCAGTG_nn'
Cuts at: 0 308 1005
Size: 308 697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 701 736 1005
Size: 701 35 269
Fragments arranged by size:
701 269 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 844 1005
Size: 844 161
EaeI y'GGCC_r
Cuts at: 0 89 236 1005
Size: 89 147 769
Fragments arranged by size:
769 147 89
EarI CTCTTCn'nnn_
Cuts at: 0 874 1005
Size: 874 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 365 542 863 898 1005
Size: 357 8 177 321 35 107
Fragments arranged by size:
357 321 177 107 35 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 57 1005
Size: 57 948
FspI TGC'GCA
Cuts at: 0 250 1005
Size: 250 755
HaeII r_GCGC'y
Cuts at: 0 8 123 144 1005
Size: 8 115 21 861
Fragments arranged by size:
861 115 21 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 290 322 1005
Size: 290 32 683
Fragments arranged by size:
683 290 32
KasI G'GCGC_C
Cuts at: 0 119 140 1005
Size: 119 21 865
Fragments arranged by size:
865 119 21
MslI CAynn'nnrTG
Cuts at: 0 238 1005
Size: 238 767
MspA1I CmG'CkG
Cuts at: 0 254 396 1005
Size: 254 142 609
Fragments arranged by size:
609 254 142
NaeI GCC'GGC
Cuts at: 0 145 1005
Size: 145 860
NarI GG'CG_CC
Cuts at: 0 120 141 1005
Size: 120 21 864
Fragments arranged by size:
864 120 21
NcoI C'CATG_G
Cuts at: 0 239 1005
Size: 239 766
NgoMIV G'CCGG_C
Cuts at: 0 143 1005
Size: 143 862
NspI r_CATG'y
Cuts at: 0 806 1005
Size: 806 199
PasI CC'CwG_GG
Cuts at: 0 102 1005
Size: 102 903
PflMI CCAn_nnn'nTGG
Cuts at: 0 841 937 1005
Size: 841 96 68
Fragments arranged by size:
841 96 68
PstI C_TGCA'G
Cuts at: 0 155 445 1005
Size: 155 290 560
Fragments arranged by size:
560 290 155
PvuII CAG'CTG
Cuts at: 0 396 1005
Size: 396 609
SapI GCTCTTCn'nnn_
Cuts at: 0 874 1005
Size: 874 131
SfcI C'TryA_G
Cuts at: 0 151 441 1005
Size: 151 290 564
Fragments arranged by size:
564 290 151
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 97 1005
Size: 97 908
SfoI GGC'GCC
Cuts at: 0 121 142 1005
Size: 121 21 863
Fragments arranged by size:
863 121 21
SgrAI Cr'CCGG_yG
Cuts at: 0 143 1005
Size: 143 862
SphI G_CATG'C
Cuts at: 0 806 1005
Size: 806 199
SspI AAT'ATT
Cuts at: 0 717 1005
Size: 717 288
StyI C'CwwG_G
Cuts at: 0 239 1005
Size: 239 766
TatI w'GTAC_w
Cuts at: 0 917 1005
Size: 917 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 50 1005
Size: 50 955
Enzymes that do cut:
AarI AcuI AhdI AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbeI BciVI BclI BglI Bme1580I BpmI
BsaHI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstEII BstYI BtgI BtsI
CspCI DrdI EaeI EarI Eco57MI EcoNI FspI HaeII
Hin4I KasI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PstI PvuII SapI SfcI SfiI
SfoI SgrAI SphI SspI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII EagI EciI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SgfI SmaI SmlI
SnaBI SpeI SrfI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI ZraI