Restriction map for
pFN21AE0399
(Linear) (Six-base) MAPSORT of: KIEE0399.seq Check: 6048 from: 1 to: 3315
KIEE0399.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3267 3315
Size: 3267 48
AccI GT'mk_AC
Cuts at: 0 1629 3315
Size: 1629 1686
Acc65I G'GTAC_C
Cuts at: 0 2184 3192 3315
Size: 2184 1008 123
Fragments arranged by size:
2184 1008 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 553 2046 2348 2525 2860 3315
Size: 238 315 1493 302 177 335 455
Fragments arranged by size:
1493 455 335 315 302 238 177
AflIII A'CryG_T
Cuts at: 0 1487 1941 2530 3315
Size: 1487 454 589 785
Fragments arranged by size:
1487 785 589 454
AgeI A'CCGG_T
Cuts at: 0 660 3315
Size: 660 2655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2669 3315
Size: 2669 646
AlwNI CAG_nnn'CTG
Cuts at: 0 2614 3315
Size: 2614 701
ApaI G_GGCC'C
Cuts at: 0 112 2300 3315
Size: 112 2188 1015
Fragments arranged by size:
2188 1015 112
ApoI r'AATT_y
Cuts at: 0 1473 1749 2016 2579 3315
Size: 1473 276 267 563 736
Fragments arranged by size:
1473 736 563 276 267
AvaI C'yCGr_G
Cuts at: 0 54 2168 3073 3188 3315
Size: 54 2114 905 115 127
Fragments arranged by size:
2114 905 127 115 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 713 746 1112 1145 3315
Size: 713 33 366 33 2170
Fragments arranged by size:
2170 713 366 33 33
BamHI G'GATC_C
Cuts at: 0 2253 3315
Size: 2253 1062
BanI G'GyrC_C
Cuts at: 0 43 346 2184 2957 3192 3315
Size: 43 303 1838 773 235 123
Fragments arranged by size:
1838 773 303 235 123 43
BanII G_rGCy'C
Cuts at: 0 55 112 136 2300 3315
Size: 55 57 24 2164 1015
Fragments arranged by size:
2164 1015 57 55 24
BbeI G_GCGC'C
Cuts at: 0 47 350 3315
Size: 47 303 2965
Fragments arranged by size:
2965 303 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 1121 3315
Size: 1121 2194
BbvCI CC'TCA_GC
Cuts at: 0 2625 3303 3315
Size: 2625 678 12
Fragments arranged by size:
2625 678 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 2472 3230 3315
Size: 2472 758 85
Fragments arranged by size:
2472 758 85
BclI T'GATC_A
Cuts at: 0 1287 3315
Size: 1287 2028
BfrBI ATG'CAT
Cuts at: 0 1363 1970 3315
Size: 1363 607 1345
Fragments arranged by size:
1363 1345 607
BglI GCCn_nnn'nGGC
Cuts at: 0 10 2629 3315
Size: 10 2619 686
Fragments arranged by size:
2619 686 10
BglII A'GATC_T
Cuts at: 0 1095 3315
Size: 1095 2220
Bme1580I G_kGCm'C
Cuts at: 0 112 2300 2960 3315
Size: 112 2188 660 355
Fragments arranged by size:
2188 660 355 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 2564 3315
Size: 2564 751
BmtI G_CTAG'C
Cuts at: 0 1049 3315
Size: 1049 2266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1433 1568 1915 1924 2290 3315
Size: 362 1071 135 347 9 366 1025
Fragments arranged by size:
1071 1025 366 362 347 135 9
Bpu10I CC'TnA_GC
Cuts at: 0 2625 3303 3315
Size: 2625 678 12
Fragments arranged by size:
2625 678 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2032 3315
Size: 2032 1283
BsaI GGTCTCn'nnnn_
Cuts at: 0 651 1804 1873 3315
Size: 651 1153 69 1442
Fragments arranged by size:
1442 1153 651 69
BsaAI yAC'GTr
Cuts at: 0 1490 3315
Size: 1490 1825
BsaBI GATnn'nnATC
Cuts at: 0 1271 3315
Size: 1271 2044
BsaHI Gr'CG_yC
Cuts at: 0 44 347 3315
Size: 44 303 2968
Fragments arranged by size:
2968 303 44
BsaWI w'CCGG_w
Cuts at: 0 660 3315
Size: 660 2655
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 160 1196 1226 1801 1831 3315
Size: 130 30 1036 30 575 30 1484
Fragments arranged by size:
1484 1036 575 130 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 859 1640 1649 2173 2194 3315
Size: 859 781 9 524 21 1121
Fragments arranged by size:
1121 859 781 524 21 9
BseYI C'CCAG_C
Cuts at: 0 149 174 232 3035 3315
Size: 149 25 58 2803 280
Fragments arranged by size:
2803 280 149 58 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 499 3315
Size: 265 234 2816
Fragments arranged by size:
2816 265 234
BsiHKAI G_wGCw'C
Cuts at: 0 1410 2862 3315
Size: 1410 1452 453
Fragments arranged by size:
1452 1410 453
BsmI GAATG_Cn'
Cuts at: 0 1972 3315
Size: 1972 1343
BsmBI CGTCTCn'nnnn_
Cuts at: 0 133 3315
Size: 133 3182
Bsp1286I G_dGCh'C
Cuts at: 0 55 112 136 1410 2300 2862 2960 3315
Size: 55 57 24 1274 890 562 98 355
Fragments arranged by size:
1274 890 562 355 98 57 55 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3267 3315
Size: 3267 48
BsrDI GCAATG_nn'
Cuts at: 0 1646 2282 3315
Size: 1646 636 1033
Fragments arranged by size:
1646 1033 636
BsrFI r'CCGG_y
Cuts at: 0 660 3315
Size: 660 2655
BsrGI T'GTAC_A
Cuts at: 0 1910 3315
Size: 1910 1405
BssSI C'ACGA_G
Cuts at: 0 2234 3315
Size: 2234 1081
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2137 2614 2802 3084 3315
Size: 2137 477 188 282 231
Fragments arranged by size:
2137 477 282 231 188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1641 2649 3130 3315
Size: 1110 531 1008 481 185
Fragments arranged by size:
1110 1008 531 481 185
BstYI r'GATC_y
Cuts at: 0 762 1095 1218 1272 2253 3315
Size: 762 333 123 54 981 1062
Fragments arranged by size:
1062 981 762 333 123 54
Bsu36I CC'TnA_GG
Cuts at: 0 1721 3315
Size: 1721 1594
BtgI C'CryG_G
Cuts at: 0 47 784 1634 3315
Size: 47 737 850 1681
Fragments arranged by size:
1681 850 737 47
BtsI GCAGTG_nn'
Cuts at: 0 1523 1597 2663 3315
Size: 1523 74 1066 652
Fragments arranged by size:
1523 1066 652 74
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2177 2212 3315
Size: 2177 35 1103
Fragments arranged by size:
2177 1103 35
EaeI y'GGCC_r
Cuts at: 0 2 113 2889 3315
Size: 2 111 2776 426
Fragments arranged by size:
2776 426 111 2
EarI CTCTTCn'nnn_
Cuts at: 0 908 933 1018 1415 1854 2324 2501 2738
Size: 908 25 85 397 439 470 177 237
Cuts at: 2738 3315
Size: 577
Fragments arranged by size:
908 577 470 439 397 237 177 85
25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 417 3266 3315
Size: 417 2849 49
Fragments arranged by size:
2849 417 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 362 553 1433 1568 1915 1924 2046
Size: 238 124 191 880 135 347 9 122
Cuts at: 2046 2290 2348 2525 2860 3315
Size: 244 58 177 335 455
Fragments arranged by size:
880 455 347 335 244 238 191 177
135 124 122 58 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1199 1280 3315
Size: 1199 81 2035
Fragments arranged by size:
2035 1199 81
EcoO109I rG'GnC_Cy
Cuts at: 0 108 1645 1899 2001 2296 2926 3184 3270
Size: 108 1537 254 102 295 630 258 86
Cuts at: 3270 3315
Size: 45
Fragments arranged by size:
1537 630 295 258 254 108 102 86
45
EcoRI G'AATT_C
Cuts at: 0 2016 3315
Size: 2016 1299
EcoRV GAT'ATC
Cuts at: 0 558 3315
Size: 558 2757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1066 1168 3315
Size: 1066 102 2147
Fragments arranged by size:
2147 1066 102
HaeII r_GCGC'y
Cuts at: 0 47 350 3315
Size: 47 303 2965
Fragments arranged by size:
2965 303 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 491 523 580 612 873 905
Size: 130 32 329 32 57 32 261 32
Cuts at: 905 1672 1704 1801 1833 2470 2502 2961 2993
Size: 767 32 97 32 637 32 459 32
Cuts at: 2993 3315
Size: 322
Fragments arranged by size:
767 637 459 329 322 261 130 97
57 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1305 1617 3315
Size: 1305 312 1698
Fragments arranged by size:
1698 1305 312
HindIII A'AGCT_T
Cuts at: 0 602 2314 3315
Size: 602 1712 1001
Fragments arranged by size:
1712 1001 602
KasI G'GCGC_C
Cuts at: 0 43 346 3315
Size: 43 303 2969
Fragments arranged by size:
2969 303 43
KpnI G_GTAC'C
Cuts at: 0 2188 3196 3315
Size: 2188 1008 119
Fragments arranged by size:
2188 1008 119
MfeI C'AATT_G
Cuts at: 0 486 3315
Size: 486 2829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1513 1916 1926 3315
Size: 1024 489 403 10 1389
Fragments arranged by size:
1389 1024 489 403 10
MscI TGG'CCA
Cuts at: 0 2891 3315
Size: 2891 424
MslI CAynn'nnrTG
Cuts at: 0 206 737 1639 1667 3315
Size: 206 531 902 28 1648
Fragments arranged by size:
1648 902 531 206 28
MspA1I CmG'CkG
Cuts at: 0 10 37 153 894 2279 2614 2629 2838
Size: 10 27 116 741 1385 335 15 209
Cuts at: 2838 3307 3315
Size: 469 8
Fragments arranged by size:
1385 741 469 335 209 116 27 15
10 8
NarI GG'CG_CC
Cuts at: 0 44 347 3315
Size: 44 303 2968
Fragments arranged by size:
2968 303 44
NcoI C'CATG_G
Cuts at: 0 784 1634 3315
Size: 784 850 1681
Fragments arranged by size:
1681 850 784
NheI G'CTAG_C
Cuts at: 0 1045 3315
Size: 1045 2270
NsiI A_TGCA'T
Cuts at: 0 1365 1972 3315
Size: 1365 607 1343
Fragments arranged by size:
1365 1343 607
NspI r_CATG'y
Cuts at: 0 286 1945 2534 3087 3159 3219 3315
Size: 286 1659 589 553 72 60 96
Fragments arranged by size:
1659 589 553 286 96 72 60
PciI A'CATG_T
Cuts at: 0 1941 2530 3315
Size: 1941 589 785
Fragments arranged by size:
1941 785 589
PflMI CCAn_nnn'nTGG
Cuts at: 0 125 3315
Size: 125 3190
PfoI T'CCnGG_A
Cuts at: 0 260 1892 2174 3315
Size: 260 1632 282 1141
Fragments arranged by size:
1632 1141 282 260
PpuMI rG'GwC_Cy
Cuts at: 0 1645 2001 2926 3184 3270 3315
Size: 1645 356 925 258 86 45
Fragments arranged by size:
1645 925 356 258 86 45
PsiI TTA'TAA
Cuts at: 0 1379 3315
Size: 1379 1936
PspOMI G'GGCC_C
Cuts at: 0 108 2296 3315
Size: 108 2188 1019
Fragments arranged by size:
2188 1019 108
PstI C_TGCA'G
Cuts at: 0 2142 2658 3264 3315
Size: 2142 516 606 51
Fragments arranged by size:
2142 606 516 51
PvuII CAG'CTG
Cuts at: 0 153 2279 2614 2838 3307 3315
Size: 153 2126 335 224 469 8
Fragments arranged by size:
2126 469 335 224 153 8
SanDI GG'GwC_CC
Cuts at: 0 3270 3315
Size: 3270 45
SapI GCTCTTCn'nnn_
Cuts at: 0 908 1415 2738 3315
Size: 908 507 1323 577
Fragments arranged by size:
1323 908 577 507
ScaI AGT'ACT
Cuts at: 0 1151 2091 3315
Size: 1151 940 1224
Fragments arranged by size:
1224 1151 940
SfcI C'TryA_G
Cuts at: 0 415 1211 2138 2654 3260 3315
Size: 415 796 927 516 606 55
Fragments arranged by size:
927 796 606 516 415 55
SfoI GGC'GCC
Cuts at: 0 45 348 3315
Size: 45 303 2967
Fragments arranged by size:
2967 303 45
SmaI CCC'GGG
Cuts at: 0 3075 3190 3315
Size: 3075 115 125
Fragments arranged by size:
3075 125 115
SmlI C'TyrA_G
Cuts at: 0 2011 3315
Size: 2011 1304
SphI G_CATG'C
Cuts at: 0 3159 3315
Size: 3159 156
SrfI GCCC'GGGC
Cuts at: 0 3075 3315
Size: 3075 240
StuI AGG'CCT
Cuts at: 0 80 3315
Size: 80 3235
StyI C'CwwG_G
Cuts at: 0 784 1103 1308 1634 2031 3315
Size: 784 319 205 326 397 1284
Fragments arranged by size:
1284 784 397 326 319 205
TatI w'GTAC_w
Cuts at: 0 1149 1910 2089 2220 3315
Size: 1149 761 179 131 1095
Fragments arranged by size:
1149 1095 761 179 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1011 1043 3315
Size: 1011 32 2272
Fragments arranged by size:
2272 1011 32
Tth111I GACn'n_nGTC
Cuts at: 0 144 3315
Size: 144 3171
XbaI T'CTAG_A
Cuts at: 0 1221 2781 3315
Size: 1221 1560 534
Fragments arranged by size:
1560 1221 534
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 782 2265 3315
Size: 225 557 1483 1050
Fragments arranged by size:
1483 1050 557 225
XmaI C'CCGG_G
Cuts at: 0 3073 3188 3315
Size: 3073 115 127
Fragments arranged by size:
3073 127 115
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AlfI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI
MslI MspA1I NarI NcoI NheI NsiI NspI PciI
PflMI PfoI PpuMI PsiI PspOMI PstI PvuII SanDI
SapI ScaI SfcI SfoI SmaI SmlI SphI SrfI
StuI StyI TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AleI AloI ApaLI
AscI AseI AsiSI AvrII BcgI BlpI BmgBI BplI
BsiEI BsiWI BspEI BspHI BsrBI BssHII BstBI BstEII
BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EcoICRI
FseI FspI FspAI HpaI MluI NaeI NdeI NgoMIV
NotI NruI PacI PasI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI
TaqII XhoI XmnI ZraI