Restriction map for
pFN21AE0293
(Linear) (Six-base) MAPSORT of: KIEE0293.seq Check: 6035 from: 1 to: 987
KIEE0293.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 0 569 987
Size: 569 418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 987
Size: 361 626
AleI CACnn'nnGTG
Cuts at: 0 136 987
Size: 136 851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 987
Size: 520 467
AlwNI CAG_nnn'CTG
Cuts at: 0 581 987
Size: 581 406
ApaI G_GGCC'C
Cuts at: 0 554 987
Size: 554 433
AvaI C'yCGr_G
Cuts at: 0 66 987
Size: 66 921
BanI G'GyrC_C
Cuts at: 0 295 374 987
Size: 295 79 613
Fragments arranged by size:
613 295 79
BanII G_rGCy'C
Cuts at: 0 73 554 678 987
Size: 73 481 124 309
Fragments arranged by size:
481 309 124 73
BbeI G_GCGC'C
Cuts at: 0 299 987
Size: 299 688
BbvCI CC'TCA_GC
Cuts at: 0 825 987
Size: 825 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 705 739 987
Size: 705 34 248
Fragments arranged by size:
705 248 34
BlpI GC'TnA_GC
Cuts at: 0 382 449 987
Size: 382 67 538
Fragments arranged by size:
538 382 67
Bme1580I G_kGCm'C
Cuts at: 0 554 987
Size: 554 433
BmrI ACTGGGnnnn_n'
Cuts at: 0 469 987
Size: 469 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 775 987
Size: 475 300 212
Fragments arranged by size:
475 300 212
Bpu10I CC'TnA_GC
Cuts at: 0 825 987
Size: 825 162
BsaHI Gr'CG_yC
Cuts at: 0 296 987
Size: 296 691
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 759 987
Size: 759 228
BseYI C'CCAG_C
Cuts at: 0 591 987
Size: 591 396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 987
Size: 410 577
BsiEI CG_ry'CG
Cuts at: 0 47 899 987
Size: 47 852 88
Fragments arranged by size:
852 88 47
BsiHKAI G_wGCw'C
Cuts at: 0 710 987
Size: 710 277
BsmBI CGTCTCn'nnnn_
Cuts at: 0 906 987
Size: 906 81
Bsp1286I G_dGCh'C
Cuts at: 0 73 554 678 710 987
Size: 73 481 124 32 277
Fragments arranged by size:
481 277 124 73 32
BsrDI GCAATG_nn'
Cuts at: 0 230 987
Size: 230 757
BsrFI r'CCGG_y
Cuts at: 0 540 987
Size: 540 447
BstEII G'GTnAC_C
Cuts at: 0 282 987
Size: 282 705
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 564 987
Size: 279 285 423
Fragments arranged by size:
423 285 279
BtgI C'CryG_G
Cuts at: 0 272 546 905 987
Size: 272 274 359 82
Fragments arranged by size:
359 274 272 82
EaeI y'GGCC_r
Cuts at: 0 44 101 234 701 987
Size: 44 57 133 467 286
Fragments arranged by size:
467 286 133 57 44
EagI C'GGCC_G
Cuts at: 0 44 987
Size: 44 943
EarI CTCTTCn'nnn_
Cuts at: 0 841 987
Size: 841 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 475 775 987
Size: 361 114 300 212
Fragments arranged by size:
361 300 212 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 922 987
Size: 922 65
HaeII r_GCGC'y
Cuts at: 0 299 754 987
Size: 299 455 233
Fragments arranged by size:
455 299 233
KasI G'GCGC_C
Cuts at: 0 295 987
Size: 295 692
MscI TGG'CCA
Cuts at: 0 236 987
Size: 236 751
MslI CAynn'nnrTG
Cuts at: 0 136 987
Size: 136 851
MspA1I CmG'CkG
Cuts at: 0 13 22 987
Size: 13 9 965
Fragments arranged by size:
965 13 9
NaeI GCC'GGC
Cuts at: 0 542 987
Size: 542 445
NarI GG'CG_CC
Cuts at: 0 296 987
Size: 296 691
NcoI C'CATG_G
Cuts at: 0 546 987
Size: 546 441
NgoMIV G'CCGG_C
Cuts at: 0 540 987
Size: 540 447
PasI CC'CwG_GG
Cuts at: 0 291 987
Size: 291 696
PfoI T'CCnGG_A
Cuts at: 0 839 987
Size: 839 148
PspOMI G'GGCC_C
Cuts at: 0 550 987
Size: 550 437
PstI C_TGCA'G
Cuts at: 0 12 718 987
Size: 12 706 269
Fragments arranged by size:
706 269 12
PvuII CAG'CTG
Cuts at: 0 13 987
Size: 13 974
SfcI C'TryA_G
Cuts at: 0 8 142 714 987
Size: 8 134 572 273
Fragments arranged by size:
572 273 134 8
SfoI GGC'GCC
Cuts at: 0 297 987
Size: 297 690
StyI C'CwwG_G
Cuts at: 0 300 546 617 951 987
Size: 300 246 71 334 36
Fragments arranged by size:
334 300 246 71 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 870 902 987
Size: 870 32 85
Fragments arranged by size:
870 85 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 561 987
Size: 561 426
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BcgI BlpI Bme1580I BmrI BpmI
Bpu10I BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BsrDI BsrFI BstEII BstXI BtgI EaeI EagI
EarI Eco57MI EcoNI HaeII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PasI PfoI PspOMI PstI
PvuII SfcI SfoI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BglI BglII
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI