Restriction map for
pFN21AE0292
(Linear) (Six-base) MAPSORT of: KIEE0292.seq Check: 2763 from: 1 to: 756
KIEE0292.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 300 756
Size: 300 456
AccI GT'mk_AC
Cuts at: 0 488 756
Size: 488 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 756
Size: 705 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 619 756
Size: 619 137
AlwNI CAG_nnn'CTG
Cuts at: 0 618 756
Size: 618 138
ApaLI G'TGCA_C
Cuts at: 0 79 508 756
Size: 79 429 248
Fragments arranged by size:
429 248 79
AvaI C'yCGr_G
Cuts at: 0 55 119 170 275 756
Size: 55 64 51 105 481
Fragments arranged by size:
481 105 64 55 51
BanI G'GyrC_C
Cuts at: 0 271 325 405 439 477 756
Size: 271 54 80 34 38 279
Fragments arranged by size:
279 271 80 54 38 34
BbeI G_GCGC'C
Cuts at: 0 275 329 443 756
Size: 275 54 114 313
Fragments arranged by size:
313 275 114 54
BbvCI CC'TCA_GC
Cuts at: 0 496 756
Size: 496 260
BglI GCCn_nnn'nGGC
Cuts at: 0 69 287 296 756
Size: 69 218 9 460
Fragments arranged by size:
460 218 69 9
Bme1580I G_kGCm'C
Cuts at: 0 83 410 482 512 756
Size: 83 327 72 30 244
Fragments arranged by size:
327 244 83 72 30
BmgBI CAC'GTC
Cuts at: 0 340 756
Size: 340 416
Bpu10I CC'TnA_GC
Cuts at: 0 496 756
Size: 496 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 523 698 756
Size: 523 175 58
Fragments arranged by size:
523 175 58
BsaHI Gr'CG_yC
Cuts at: 0 227 272 326 440 485 644 756
Size: 227 45 54 114 45 159 112
Fragments arranged by size:
227 159 114 112 54 45 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 756
Size: 60 30 666
Fragments arranged by size:
666 60 30
BseYI C'CCAG_C
Cuts at: 0 586 756
Size: 586 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 456 523 756
Size: 364 92 67 233
Fragments arranged by size:
364 233 92 67
BsiEI CG_ry'CG
Cuts at: 0 569 675 746 756
Size: 569 106 71 10
Fragments arranged by size:
569 106 71 10
BsiHKAI G_wGCw'C
Cuts at: 0 83 512 575 701 756
Size: 83 429 63 126 55
Fragments arranged by size:
429 126 83 63 55
Bsp1286I G_dGCh'C
Cuts at: 0 83 410 482 512 575 701 756
Size: 83 327 72 30 63 126 55
Fragments arranged by size:
327 126 83 72 63 55 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 545 756
Size: 300 245 211
Fragments arranged by size:
300 245 211
BsrBI CCG'CTC
Cuts at: 0 251 756
Size: 251 505
BsrFI r'CCGG_y
Cuts at: 0 223 241 365 554 741 756
Size: 223 18 124 189 187 15
Fragments arranged by size:
223 189 187 124 18 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 546 756
Size: 546 210
BtgI C'CryG_G
Cuts at: 0 41 359 455 563 756
Size: 41 318 96 108 193
Fragments arranged by size:
318 193 108 96 41
BtsI GCAGTG_nn'
Cuts at: 0 28 81 756
Size: 28 53 675
Fragments arranged by size:
675 53 28
EaeI y'GGCC_r
Cuts at: 0 452 566 726 743 756
Size: 452 114 160 17 13
Fragments arranged by size:
452 160 114 17 13
EagI C'GGCC_G
Cuts at: 0 566 743 756
Size: 566 177 13
Fragments arranged by size:
566 177 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 756
Size: 705 51
EcoO109I rG'GnC_Cy
Cuts at: 0 47 71 135 150 279 375 756
Size: 47 24 64 15 129 96 381
Fragments arranged by size:
381 129 96 64 47 24 15
FspI TGC'GCA
Cuts at: 0 658 756
Size: 658 98
FspAI rTGC'GCAy
Cuts at: 0 658 756
Size: 658 98
HaeII r_GCGC'y
Cuts at: 0 275 329 443 756
Size: 275 54 114 313
Fragments arranged by size:
313 275 114 54
KasI G'GCGC_C
Cuts at: 0 271 325 439 756
Size: 271 54 114 317
Fragments arranged by size:
317 271 114 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 756
Size: 413 343
MspA1I CmG'CkG
Cuts at: 0 457 565 725 756
Size: 457 108 160 31
Fragments arranged by size:
457 160 108 31
NaeI GCC'GGC
Cuts at: 0 225 243 367 743 756
Size: 225 18 124 376 13
Fragments arranged by size:
376 225 124 18 13
NarI GG'CG_CC
Cuts at: 0 272 326 440 756
Size: 272 54 114 316
Fragments arranged by size:
316 272 114 54
NgoMIV G'CCGG_C
Cuts at: 0 223 241 365 741 756
Size: 223 18 124 376 15
Fragments arranged by size:
376 223 124 18 15
NspI r_CATG'y
Cuts at: 0 663 756
Size: 663 93
PpuMI rG'GwC_Cy
Cuts at: 0 135 150 756
Size: 135 15 606
Fragments arranged by size:
606 135 15
PspXI vC'TCGA_Gb
Cuts at: 0 170 756
Size: 170 586
PstI C_TGCA'G
Cuts at: 0 287 756
Size: 287 469
SacII CC_GC'GG
Cuts at: 0 458 566 756
Size: 458 108 190
Fragments arranged by size:
458 190 108
SbfI CC_TGCA'GG
Cuts at: 0 287 756
Size: 287 469
SexAI A'CCwGG_T
Cuts at: 0 731 756
Size: 731 25
SfcI C'TryA_G
Cuts at: 0 185 283 756
Size: 185 98 473
Fragments arranged by size:
473 185 98
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 287 296 756
Size: 287 9 460
Fragments arranged by size:
460 287 9
SfoI GGC'GCC
Cuts at: 0 273 327 441 756
Size: 273 54 114 315
Fragments arranged by size:
315 273 114 54
SmaI CCC'GGG
Cuts at: 0 57 121 277 756
Size: 57 64 156 479
Fragments arranged by size:
479 156 64 57
SmlI C'TyrA_G
Cuts at: 0 170 756
Size: 170 586
SrfI GCCC'GGGC
Cuts at: 0 57 756
Size: 57 699
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 756
Size: 642 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 60 92 756
Size: 60 32 664
Fragments arranged by size:
664 60 32
Tth111I GACn'n_nGTC
Cuts at: 0 646 756
Size: 646 110
XhoI C'TCGA_G
Cuts at: 0 170 756
Size: 170 586
XmaI C'CCGG_G
Cuts at: 0 55 119 275 756
Size: 55 64 156 481
Fragments arranged by size:
481 156 64 55
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaLI AvaI BanI
BbeI BbvCI BglI Bme1580I BmgBI Bpu10I BsaI BsaHI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BstAPI BtgI BtsI EaeI EagI Eco57MI EcoO109I
FspI FspAI HaeII KasI MmeI MspA1I NaeI NarI
NgoMIV NspI PpuMI PspXI PstI SacII SbfI SexAI
SfcI SfiI SfoI SmaI SmlI SrfI TaqII TstI
Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI ScaI SgfI SgrAI SnaBI SpeI SphI
SspI StuI StyI SwaI TatI XbaI XcmI XmnI
ZraI