Restriction map for
pFN21AE0270
(Linear) (Six-base) MAPSORT of: KIEE0270.seq Check: 2760 from: 1 to: 732
KIEE0270.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 732
Size: 416 316
AfeI AGC'GCT
Cuts at: 0 181 732
Size: 181 551
AflIII A'CryG_T
Cuts at: 0 717 732
Size: 717 15
AhdI GACnn_n'nnGTC
Cuts at: 0 718 732
Size: 718 14
AlwNI CAG_nnn'CTG
Cuts at: 0 682 732
Size: 682 50
BamHI G'GATC_C
Cuts at: 0 3 732
Size: 3 729
BanI G'GyrC_C
Cuts at: 0 10 732
Size: 10 722
BanII G_rGCy'C
Cuts at: 0 61 732
Size: 61 671
BbeI G_GCGC'C
Cuts at: 0 14 732
Size: 14 718
BbvCI CC'TCA_GC
Cuts at: 0 324 732
Size: 324 408
BciVI GTATCCnnnnn_n'
Cuts at: 0 670 732
Size: 670 62
BglI GCCn_nnn'nGGC
Cuts at: 0 108 732
Size: 108 624
BglII A'GATC_T
Cuts at: 0 281 732
Size: 281 451
BmrI ACTGGGnnnn_n'
Cuts at: 0 542 732
Size: 542 190
Bpu10I CC'TnA_GC
Cuts at: 0 324 732
Size: 324 408
BsaHI Gr'CG_yC
Cuts at: 0 11 732
Size: 11 721
BsiEI CG_ry'CG
Cuts at: 0 116 732
Size: 116 616
Bsp1286I G_dGCh'C
Cuts at: 0 61 732
Size: 61 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 120 286 732
Size: 120 166 446
Fragments arranged by size:
446 166 120
BsrFI r'CCGG_y
Cuts at: 0 111 732
Size: 111 621
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 732
Size: 174 558
BstYI r'GATC_y
Cuts at: 0 3 281 732
Size: 3 278 451
Fragments arranged by size:
451 278 3
BtgI C'CryG_G
Cuts at: 0 150 221 732
Size: 150 71 511
Fragments arranged by size:
511 150 71
EaeI y'GGCC_r
Cuts at: 0 78 113 732
Size: 78 35 619
Fragments arranged by size:
619 78 35
EagI C'GGCC_G
Cuts at: 0 113 732
Size: 113 619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 732
Size: 416 316
EcoRV GAT'ATC
Cuts at: 0 369 732
Size: 369 363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 585 732
Size: 585 147
FseI GG_CCGG'CC
Cuts at: 0 115 732
Size: 115 617
HaeII r_GCGC'y
Cuts at: 0 14 70 183 732
Size: 14 56 113 549
Fragments arranged by size:
549 113 56 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 704 732
Size: 266 32 406 28
Fragments arranged by size:
406 266 32 28
HincII GTy'rAC
Cuts at: 0 231 732
Size: 231 501
KasI G'GCGC_C
Cuts at: 0 10 732
Size: 10 722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 449 732
Size: 438 11 283
Fragments arranged by size:
438 283 11
MslI CAynn'nnrTG
Cuts at: 0 535 732
Size: 535 197
MspA1I CmG'CkG
Cuts at: 0 152 732
Size: 152 580
NaeI GCC'GGC
Cuts at: 0 113 732
Size: 113 619
NarI GG'CG_CC
Cuts at: 0 11 732
Size: 11 721
NgoMIV G'CCGG_C
Cuts at: 0 111 732
Size: 111 621
NspI r_CATG'y
Cuts at: 0 721 732
Size: 721 11
PciI A'CATG_T
Cuts at: 0 717 732
Size: 717 15
PstI C_TGCA'G
Cuts at: 0 23 732
Size: 23 709
SacII CC_GC'GG
Cuts at: 0 153 732
Size: 153 579
SfcI C'TryA_G
Cuts at: 0 19 248 732
Size: 19 229 484
Fragments arranged by size:
484 229 19
SfoI GGC'GCC
Cuts at: 0 12 732
Size: 12 720
StuI AGG'CCT
Cuts at: 0 208 732
Size: 208 524
StyI C'CwwG_G
Cuts at: 0 344 560 732
Size: 344 216 172
Fragments arranged by size:
344 216 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 732
Size: 214 518
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlwNI BamHI BanI BanII
BbeI BbvCI BciVI BglI BglII BmrI Bpu10I BsaHI
BsiEI Bsp1286I BspMI BsrFI BstXI BstYI BtgI EaeI
EagI Eco57MI EcoRV FalI FseI HaeII Hin4I HincII
KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PstI SacII SfcI SfoI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BcgI BclI BfrBI BlpI
Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI