Restriction map for
pFN21AE0237
(Linear) (Six-base) MAPSORT of: KIEE0237.seq Check: 163 from: 1 to: 474
KIEE0237.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 474
Size: 389 85
ApaI G_GGCC'C
Cuts at: 0 74 256 474
Size: 74 182 218
Fragments arranged by size:
218 182 74
AvaI C'yCGr_G
Cuts at: 0 52 135 348 397 474
Size: 52 83 213 49 77
Fragments arranged by size:
213 83 77 52 49
BanI G'GyrC_C
Cuts at: 0 111 156 183 474
Size: 111 45 27 291
Fragments arranged by size:
291 111 45 27
BanII G_rGCy'C
Cuts at: 0 74 256 266 474
Size: 74 182 10 208
Fragments arranged by size:
208 182 74 10
BbeI G_GCGC'C
Cuts at: 0 160 187 474
Size: 160 27 287
Fragments arranged by size:
287 160 27
BglI GCCn_nnn'nGGC
Cuts at: 0 241 402 474
Size: 241 161 72
Fragments arranged by size:
241 161 72
Bme1580I G_kGCm'C
Cuts at: 0 74 256 474
Size: 74 182 218
Fragments arranged by size:
218 182 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 474
Size: 109 365
BsaHI Gr'CG_yC
Cuts at: 0 157 184 474
Size: 157 27 290
Fragments arranged by size:
290 157 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 474
Size: 166 308
BseYI C'CCAG_C
Cuts at: 0 172 279 474
Size: 172 107 195
Fragments arranged by size:
195 172 107
BsiEI CG_ry'CG
Cuts at: 0 128 165 305 387 474
Size: 128 37 140 82 87
Fragments arranged by size:
140 128 87 82 37
BsiHKAI G_wGCw'C
Cuts at: 0 337 474
Size: 337 137
BsmBI CGTCTCn'nnnn_
Cuts at: 0 199 474
Size: 199 275
Bsp1286I G_dGCh'C
Cuts at: 0 74 256 266 337 474
Size: 74 182 10 71 137
Fragments arranged by size:
182 137 74 71 10
BsrBI CCG'CTC
Cuts at: 0 79 356 459 474
Size: 79 277 103 15
Fragments arranged by size:
277 103 79 15
BsrDI GCAATG_nn'
Cuts at: 0 366 474
Size: 366 108
BsrFI r'CCGG_y
Cuts at: 0 104 114 405 474
Size: 104 10 291 69
Fragments arranged by size:
291 104 69 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 474
Size: 180 294
BtgI C'CryG_G
Cuts at: 0 65 146 179 299 474
Size: 65 81 33 120 175
Fragments arranged by size:
175 120 81 65 33
EaeI y'GGCC_r
Cuts at: 0 125 162 233 474
Size: 125 37 71 241
Fragments arranged by size:
241 125 71 37
EagI C'GGCC_G
Cuts at: 0 125 162 474
Size: 125 37 312
Fragments arranged by size:
312 125 37
EarI CTCTTCn'nnn_
Cuts at: 0 428 474
Size: 428 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 389 474
Size: 109 280 85
Fragments arranged by size:
280 109 85
EcoO109I rG'GnC_Cy
Cuts at: 0 70 98 132 252 253 474
Size: 70 28 34 120 1 221
Fragments arranged by size:
221 120 70 34 28 1
FspI TGC'GCA
Cuts at: 0 324 474
Size: 324 150
HaeII r_GCGC'y
Cuts at: 0 160 187 474
Size: 160 27 287
Fragments arranged by size:
287 160 27
KasI G'GCGC_C
Cuts at: 0 156 183 474
Size: 156 27 291
Fragments arranged by size:
291 156 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 474
Size: 315 159
MspA1I CmG'CkG
Cuts at: 0 8 67 148 301 383 474
Size: 8 59 81 153 82 91
Fragments arranged by size:
153 91 82 81 59 8
NaeI GCC'GGC
Cuts at: 0 106 407 474
Size: 106 301 67
Fragments arranged by size:
301 106 67
NarI GG'CG_CC
Cuts at: 0 157 184 474
Size: 157 27 290
Fragments arranged by size:
290 157 27
NgoMIV G'CCGG_C
Cuts at: 0 104 405 474
Size: 104 301 69
Fragments arranged by size:
301 104 69
PpuMI rG'GwC_Cy
Cuts at: 0 98 132 474
Size: 98 34 342
Fragments arranged by size:
342 98 34
PspOMI G'GGCC_C
Cuts at: 0 70 252 474
Size: 70 182 222
Fragments arranged by size:
222 182 70
SacII CC_GC'GG
Cuts at: 0 68 149 302 474
Size: 68 81 153 172
Fragments arranged by size:
172 153 81 68
SanDI GG'GwC_CC
Cuts at: 0 98 474
Size: 98 376
ScaI AGT'ACT
Cuts at: 0 466 474
Size: 466 8
SfoI GGC'GCC
Cuts at: 0 158 185 474
Size: 158 27 289
Fragments arranged by size:
289 158 27
SgrAI Cr'CCGG_yG
Cuts at: 0 104 474
Size: 104 370
SmaI CCC'GGG
Cuts at: 0 137 350 474
Size: 137 213 124
Fragments arranged by size:
213 137 124
TatI w'GTAC_w
Cuts at: 0 464 474
Size: 464 10
XmaI C'CCGG_G
Cuts at: 0 135 348 474
Size: 135 213 126
Fragments arranged by size:
213 135 126
Enzymes that do cut:
AcuI ApaI AvaI BanI BanII BbeI BglI Bme1580I
BpmI BsaHI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BstXI BtgI EaeI EagI EarI
Eco57MI EcoO109I FspI HaeII KasI MmeI MspA1I NaeI
NarI NgoMIV PpuMI PspOMI SacII SanDI ScaI SfoI
SgrAI SmaI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BsgI BsiWI BsmI BspEI BspHI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI SexAI SfcI SfiI SgfI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI