Restriction map for
pFN21AE0233
(Linear) (Six-base) MAPSORT of: KIEE0233.seq Check: 5378 from: 1 to: 1221
KIEE0233.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AccI GT'mk_AC
Cuts at: 0 578 1221
Size: 578 643
AclI AA'CG_TT
Cuts at: 0 882 1221
Size: 882 339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 547 691 1221
Size: 480 67 144 530
Fragments arranged by size:
530 480 144 67
AfeI AGC'GCT
Cuts at: 0 139 1003 1221
Size: 139 864 218
Fragments arranged by size:
864 218 139
AflIII A'CryG_T
Cuts at: 0 1040 1221
Size: 1040 181
AlwNI CAG_nnn'CTG
Cuts at: 0 309 339 1221
Size: 309 30 882
Fragments arranged by size:
882 309 30
ApaI G_GGCC'C
Cuts at: 0 82 1109 1221
Size: 82 1027 112
Fragments arranged by size:
1027 112 82
AvaI C'yCGr_G
Cuts at: 0 59 169 376 1221
Size: 59 110 207 845
Fragments arranged by size:
845 207 110 59
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 642 675 1221
Size: 642 33 546
Fragments arranged by size:
642 546 33
BanI G'GyrC_C
Cuts at: 0 165 273 318 1221
Size: 165 108 45 903
Fragments arranged by size:
903 165 108 45
BanII G_rGCy'C
Cuts at: 0 82 1109 1221
Size: 82 1027 112
Fragments arranged by size:
1027 112 82
BbeI G_GCGC'C
Cuts at: 0 169 277 322 1221
Size: 169 108 45 899
Fragments arranged by size:
899 169 108 45
BciVI GTATCCnnnnn_n'
Cuts at: 0 670 1221
Size: 670 551
BglII A'GATC_T
Cuts at: 0 245 587 1221
Size: 245 342 634
Fragments arranged by size:
634 342 245
Bme1580I G_kGCm'C
Cuts at: 0 82 1109 1221
Size: 82 1027 112
Fragments arranged by size:
1027 112 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 620 771 785 1221
Size: 620 151 14 436
Fragments arranged by size:
620 436 151 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 702 1221
Size: 702 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 126 409 1221
Size: 67 59 283 812
Fragments arranged by size:
812 283 67 59
Bpu10I CC'TnA_GC
Cuts at: 0 693 998 1221
Size: 693 305 223
Fragments arranged by size:
693 305 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 270 906 1221
Size: 250 20 636 315
Fragments arranged by size:
636 315 250 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 1221
Size: 412 809
BsaHI Gr'CG_yC
Cuts at: 0 166 274 319 648 1221
Size: 166 108 45 329 573
Fragments arranged by size:
573 329 166 108 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 1006 1036 1221
Size: 385 30 591 30 185
Fragments arranged by size:
591 385 185 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 406 1221
Size: 28 378 815
Fragments arranged by size:
815 378 28
BseYI C'CCAG_C
Cuts at: 0 24 450 533 567 977 1102 1221
Size: 24 426 83 34 410 125 119
Fragments arranged by size:
426 410 125 119 83 34 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1096 1221
Size: 667 429 125
Fragments arranged by size:
667 429 125
BsiEI CG_ry'CG
Cuts at: 0 74 1221
Size: 74 1147
BsiHKAI G_wGCw'C
Cuts at: 0 8 920 1221
Size: 8 912 301
Fragments arranged by size:
912 301 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 436 1221
Size: 436 785
Bsp1286I G_dGCh'C
Cuts at: 0 8 82 920 1109 1221
Size: 8 74 838 189 112
Fragments arranged by size:
838 189 112 74 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 590 1221
Size: 590 631
BsrBI CCG'CTC
Cuts at: 0 665 1221
Size: 665 556
BsrFI r'CCGG_y
Cuts at: 0 146 644 1221
Size: 146 498 577
Fragments arranged by size:
577 498 146
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 591 1221
Size: 309 282 630
Fragments arranged by size:
630 309 282
BstYI r'GATC_y
Cuts at: 0 245 335 587 1184 1221
Size: 245 90 252 597 37
Fragments arranged by size:
597 252 245 90 37
BtgI C'CryG_G
Cuts at: 0 83 161 1221
Size: 83 78 1060
Fragments arranged by size:
1060 83 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 975 1221
Size: 975 246
DrdI GACnn_nn'nnGTC
Cuts at: 0 808 1221
Size: 808 413
EaeI y'GGCC_r
Cuts at: 0 71 148 1026 1221
Size: 71 77 878 195
Fragments arranged by size:
878 195 77 71
EagI C'GGCC_G
Cuts at: 0 71 1221
Size: 71 1150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 653 733 906 1221
Size: 653 80 173 315
Fragments arranged by size:
653 315 173 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 126 409 480 547 691 1221
Size: 67 59 283 71 67 144 530
Fragments arranged by size:
530 283 144 71 67 67 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 1221
Size: 391 830
EcoO109I rG'GnC_Cy
Cuts at: 0 78 79 1106 1221
Size: 78 1 1027 115
Fragments arranged by size:
1027 115 78 1
EcoRV GAT'ATC
Cuts at: 0 946 1221
Size: 946 275
HaeII r_GCGC'y
Cuts at: 0 141 169 277 322 1005 1221
Size: 141 28 108 45 683 216
Fragments arranged by size:
683 216 141 108 45 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 935 936 967 1221
Size: 904 31 1 31 254
Fragments arranged by size:
904 254 31 31 1
KasI G'GCGC_C
Cuts at: 0 165 273 318 1221
Size: 165 108 45 903
Fragments arranged by size:
903 165 108 45
MslI CAynn'nnrTG
Cuts at: 0 430 1045 1126 1198 1221
Size: 430 615 81 72 23
Fragments arranged by size:
615 430 81 72 23
MspA1I CmG'CkG
Cuts at: 0 18 85 118 306 327 537 737 935
Size: 18 67 33 188 21 210 200 198
Cuts at: 935 1221
Size: 286
Fragments arranged by size:
286 210 200 198 188 67 33 21
18
NaeI GCC'GGC
Cuts at: 0 148 646 1221
Size: 148 498 575
Fragments arranged by size:
575 498 148
NarI GG'CG_CC
Cuts at: 0 166 274 319 1221
Size: 166 108 45 902
Fragments arranged by size:
902 166 108 45
NgoMIV G'CCGG_C
Cuts at: 0 146 644 1221
Size: 146 498 577
Fragments arranged by size:
577 498 146
NspI r_CATG'y
Cuts at: 0 1044 1146 1221
Size: 1044 102 75
Fragments arranged by size:
1044 102 75
PasI CC'CwG_GG
Cuts at: 0 819 1221
Size: 819 402
PciI A'CATG_T
Cuts at: 0 1040 1221
Size: 1040 181
PfoI T'CCnGG_A
Cuts at: 0 338 386 1221
Size: 338 48 835
Fragments arranged by size:
835 338 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1221
Size: 1027 32 162
Fragments arranged by size:
1027 162 32
PspOMI G'GGCC_C
Cuts at: 0 78 1105 1221
Size: 78 1027 116
Fragments arranged by size:
1027 116 78
PstI C_TGCA'G
Cuts at: 0 332 374 587 1221
Size: 332 42 213 634
Fragments arranged by size:
634 332 213 42
PvuII CAG'CTG
Cuts at: 0 306 537 737 1221
Size: 306 231 200 484
Fragments arranged by size:
484 306 231 200
SacII CC_GC'GG
Cuts at: 0 86 1221
Size: 86 1135
SfcI C'TryA_G
Cuts at: 0 328 370 583 1221
Size: 328 42 213 638
Fragments arranged by size:
638 328 213 42
SfoI GGC'GCC
Cuts at: 0 167 275 320 1221
Size: 167 108 45 901
Fragments arranged by size:
901 167 108 45
SmaI CCC'GGG
Cuts at: 0 61 1221
Size: 61 1160
SmlI C'TyrA_G
Cuts at: 0 249 265 921 1221
Size: 249 16 656 300
Fragments arranged by size:
656 300 249 16
StyI C'CwwG_G
Cuts at: 0 314 503 1221
Size: 314 189 718
Fragments arranged by size:
718 314 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 531 1221
Size: 531 690
TatI w'GTAC_w
Cuts at: 0 456 1037 1221
Size: 456 581 184
Fragments arranged by size:
581 456 184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 758 1221
Size: 758 463
XmaI C'CCGG_G
Cuts at: 0 59 1221
Size: 59 1162
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AlwNI ApaI AvaI
BaeI BanI BanII BbeI BciVI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstYI BtgI BtgZI DrdI EaeI EagI
EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PpiI PspOMI PstI PvuII SacII SfcI SfoI
SmaI SmlI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbsI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI
BmtI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII EarI EcoICRI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI