(Linear) (Six-base) MAPSORT of: KIEE0189.seq  Check: 7592  from: 1  to: 663



KIEE0189.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     607     663 
   Size:        607      56
 
ApoI r'AATT_y

Cuts at:      0     340     663 
   Size:        340     323
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     121     155     663 
   Size:        121      34     508

  Fragments arranged by size: 

                 508     121      34
 
BglII A'GATC_T

Cuts at:      0     486     663 
   Size:        486     177
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     245     663 
   Size:        245     418
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     663 
   Size:         54     609
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     663 
   Size:        363     300
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     634     663 
   Size:        634      29
 
BseYI C'CCAG_C

Cuts at:      0     390     663 
   Size:        390     273
 
BsrDI GCAATG_nn'

Cuts at:      0     165     663 
   Size:        165     498
 
BstYI r'GATC_y

Cuts at:      0     486     663 
   Size:        486     177
 
DraI TTT'AAA

Cuts at:      0     345     663 
   Size:        345     318
 
EarI CTCTTCn'nnn_

Cuts at:      0     577     663 
   Size:        577      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     663 
   Size:         54     609
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     415     447     663 
   Size:        415      32     216

  Fragments arranged by size: 

                 415     216      32
 
MspA1I CmG'CkG

Cuts at:      0     604     663 
   Size:        604      59
 
PstI C_TGCA'G

Cuts at:      0     335     589     663 
   Size:        335     254      74

  Fragments arranged by size: 

                 335     254      74
 
PvuII CAG'CTG

Cuts at:      0     604     663 
   Size:        604      59
 
SfcI C'TryA_G

Cuts at:      0     331     585     663 
   Size:        331     254      78

  Fragments arranged by size: 

                 331     254      78
 
SmlI C'TyrA_G

Cuts at:      0     378     663 
   Size:        378     285
 
Tth111I GACn'n_nGTC

Cuts at:      0     251     663 
   Size:        251     412
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     121     663 
   Size:        121     542
 

 Enzymes that do cut:

    AlwNI     ApoI     BcgI    BglII     BmrI     BpmI    BpuEI    BseRI
    BseYI    BsrDI    BstYI     DraI     EarI  Eco57MI    Hin4I   MspA1I
     PstI    PvuII     SfcI     SmlI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI