Restriction map for
pFN21AE0088
(Linear) (Six-base) MAPSORT of: KIEE0088.seq Check: 2416 from: 1 to: 2715
KIEE0088.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AccI GT'mk_AC
Cuts at: 0 555 2715
Size: 555 2160
Acc65I G'GTAC_C
Cuts at: 0 1639 2715
Size: 1639 1076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 2626 2715
Size: 977 1649 89
Fragments arranged by size:
1649 977 89
AflII C'TTAA_G
Cuts at: 0 718 2715
Size: 718 1997
AflIII A'CryG_T
Cuts at: 0 866 2715
Size: 866 1849
AleI CACnn'nnGTG
Cuts at: 0 1561 2715
Size: 1561 1154
AlwNI CAG_nnn'CTG
Cuts at: 0 709 729 2715
Size: 709 20 1986
Fragments arranged by size:
1986 709 20
ApaI G_GGCC'C
Cuts at: 0 1473 2715
Size: 1473 1242
ApoI r'AATT_y
Cuts at: 0 1218 2521 2715
Size: 1218 1303 194
Fragments arranged by size:
1303 1218 194
AvaI C'yCGr_G
Cuts at: 0 28 922 2413 2715
Size: 28 894 1491 302
Fragments arranged by size:
1491 894 302 28
AvrII C'CTAG_G
Cuts at: 0 1605 2715
Size: 1605 1110
BamHI G'GATC_C
Cuts at: 0 2171 2715
Size: 2171 544
BanI G'GyrC_C
Cuts at: 0 1061 1639 2715
Size: 1061 578 1076
Fragments arranged by size:
1076 1061 578
BanII G_rGCy'C
Cuts at: 0 35 566 1473 1854 2715
Size: 35 531 907 381 861
Fragments arranged by size:
907 861 531 381 35
BbeI G_GCGC'C
Cuts at: 0 1065 2715
Size: 1065 1650
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 2426 2715
Size: 539 1887 289
Fragments arranged by size:
1887 539 289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 357 391 2715
Size: 34 323 34 2324
Fragments arranged by size:
2324 323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2677 2715
Size: 2677 38
BclI T'GATC_A
Cuts at: 0 1088 2715
Size: 1088 1627
BglI GCCn_nnn'nGGC
Cuts at: 0 1963 2715
Size: 1963 752
Bme1580I G_kGCm'C
Cuts at: 0 60 1473 2442 2715
Size: 60 1413 969 273
Fragments arranged by size:
1413 969 273 60
BmgBI CAC'GTC
Cuts at: 0 1800 2715
Size: 1800 915
BmrI ACTGGGnnnn_n'
Cuts at: 0 1127 2602 2715
Size: 1127 1475 113
Fragments arranged by size:
1475 1127 113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2708 2715
Size: 2708 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1664 2715
Size: 564 1100 1051
Fragments arranged by size:
1100 1051 564
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1170 2715
Size: 631 539 1545
Fragments arranged by size:
1545 631 539
BsaAI yAC'GTr
Cuts at: 0 867 1563 2715
Size: 867 696 1152
Fragments arranged by size:
1152 867 696
BsaBI GATnn'nnATC
Cuts at: 0 1163 2715
Size: 1163 1552
BsaHI Gr'CG_yC
Cuts at: 0 1062 2715
Size: 1062 1653
BsaWI w'CCGG_w
Cuts at: 0 1625 2715
Size: 1625 1090
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 273 303 2715
Size: 273 30 2412
Fragments arranged by size:
2412 273 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 52 330 1535 2715
Size: 7 45 278 1205 1180
Fragments arranged by size:
1205 1180 278 45 7
BseYI C'CCAG_C
Cuts at: 0 23 53 1016 2715
Size: 23 30 963 1699
Fragments arranged by size:
1699 963 30 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2715
Size: 376 2339
BsiHKAI G_wGCw'C
Cuts at: 0 8 566 1703 1854 2186 2715
Size: 8 558 1137 151 332 529
Fragments arranged by size:
1137 558 529 332 151 8
BsmI GAATG_Cn'
Cuts at: 0 1616 2715
Size: 1616 1099
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2679 2715
Size: 2679 36
Bsp1286I G_dGCh'C
Cuts at: 0 8 35 60 566 1473 1703 1854 2186
Size: 8 27 25 506 907 230 151 332
Cuts at: 2186 2442 2715
Size: 256 273
Fragments arranged by size:
907 506 332 273 256 230 151 27
25 8
BspHI T'CATG_A
Cuts at: 0 1598 2123 2715
Size: 1598 525 592
Fragments arranged by size:
1598 592 525
BsrDI GCAATG_nn'
Cuts at: 0 375 995 2715
Size: 375 620 1720
Fragments arranged by size:
1720 620 375
BsrGI T'GTAC_A
Cuts at: 0 983 1337 2715
Size: 983 354 1378
Fragments arranged by size:
1378 983 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1317 1688 2442 2715
Size: 1317 371 754 273
Fragments arranged by size:
1317 754 371 273
BstYI r'GATC_y
Cuts at: 0 486 795 2171 2715
Size: 486 309 1376 544
Fragments arranged by size:
1376 544 486 309
BtgI C'CryG_G
Cuts at: 0 893 1057 2715
Size: 893 164 1658
Fragments arranged by size:
1658 893 164
BtsI GCAGTG_nn'
Cuts at: 0 1045 1544 2715
Size: 1045 499 1171
Fragments arranged by size:
1171 1045 499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2205 2240 2715
Size: 2205 35 475
Fragments arranged by size:
2205 475 35
EaeI y'GGCC_r
Cuts at: 0 992 1286 1955 2715
Size: 992 294 669 760
Fragments arranged by size:
992 760 669 294
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 2715
Size: 595 2120
EcoICRI GAG'CTC
Cuts at: 0 564 1852 2715
Size: 564 1288 863
Fragments arranged by size:
1288 863 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 977 1664 2626 2715
Size: 564 413 687 962 89
Fragments arranged by size:
962 687 564 413 89
EcoO109I rG'GnC_Cy
Cuts at: 0 909 1469 2268 2715
Size: 909 560 799 447
Fragments arranged by size:
909 799 560 447
EcoRI G'AATT_C
Cuts at: 0 1218 2521 2715
Size: 1218 1303 194
Fragments arranged by size:
1303 1218 194
HaeII r_GCGC'y
Cuts at: 0 1065 2715
Size: 1065 1650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 471 503 874 906 2715
Size: 471 32 371 32 1809
Fragments arranged by size:
1809 471 371 32 32
KasI G'GCGC_C
Cuts at: 0 1061 2715
Size: 1061 1654
KpnI G_GTAC'C
Cuts at: 0 1643 2715
Size: 1643 1072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1827 1887 2715
Size: 1247 580 60 828
Fragments arranged by size:
1247 828 580 60
MscI TGG'CCA
Cuts at: 0 994 1288 2715
Size: 994 294 1427
Fragments arranged by size:
1427 994 294
MslI CAynn'nnrTG
Cuts at: 0 739 1561 2715
Size: 739 822 1154
Fragments arranged by size:
1154 822 739
MspA1I CmG'CkG
Cuts at: 0 53 297 401 612 709 1020 1724 2715
Size: 53 244 104 211 97 311 704 991
Fragments arranged by size:
991 704 311 244 211 104 97 53
NarI GG'CG_CC
Cuts at: 0 1062 2715
Size: 1062 1653
NcoI C'CATG_G
Cuts at: 0 893 1057 2715
Size: 893 164 1658
Fragments arranged by size:
1658 893 164
NdeI CA'TA_TG
Cuts at: 0 2375 2715
Size: 2375 340
NspI r_CATG'y
Cuts at: 0 290 2715
Size: 290 2425
PasI CC'CwG_GG
Cuts at: 0 2442 2715
Size: 2442 273
PfoI T'CCnGG_A
Cuts at: 0 482 2715
Size: 482 2233
PmlI CAC'GTG
Cuts at: 0 867 2715
Size: 867 1848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2549 2581 2715
Size: 2549 32 134
Fragments arranged by size:
2549 134 32
PspOMI G'GGCC_C
Cuts at: 0 1469 2715
Size: 1469 1246
PstI C_TGCA'G
Cuts at: 0 908 1040 2715
Size: 908 132 1675
Fragments arranged by size:
1675 908 132
PvuII CAG'CTG
Cuts at: 0 53 297 612 1020 1724 2715
Size: 53 244 315 408 704 991
Fragments arranged by size:
991 704 408 315 244 53
SacI G_AGCT'C
Cuts at: 0 566 1854 2715
Size: 566 1288 861
Fragments arranged by size:
1288 861 566
ScaI AGT'ACT
Cuts at: 0 107 754 2715
Size: 107 647 1961
Fragments arranged by size:
1961 647 107
SfcI C'TryA_G
Cuts at: 0 807 904 1036 2715
Size: 807 97 132 1679
Fragments arranged by size:
1679 807 132 97
SfoI GGC'GCC
Cuts at: 0 1063 2715
Size: 1063 1652
SmaI CCC'GGG
Cuts at: 0 30 2715
Size: 30 2685
SmlI C'TyrA_G
Cuts at: 0 646 718 1149 2715
Size: 646 72 431 1566
Fragments arranged by size:
1566 646 431 72
SpeI A'CTAG_T
Cuts at: 0 1446 2715
Size: 1446 1269
SrfI GCCC'GGGC
Cuts at: 0 30 2715
Size: 30 2685
SspI AAT'ATT
Cuts at: 0 591 2457 2715
Size: 591 1866 258
Fragments arranged by size:
1866 591 258
StyI C'CwwG_G
Cuts at: 0 893 1057 1605 2715
Size: 893 164 548 1110
Fragments arranged by size:
1110 893 548 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 930 1793 2715
Size: 763 167 863 922
Fragments arranged by size:
922 863 763 167
TatI w'GTAC_w
Cuts at: 0 105 752 983 1337 1728 2715
Size: 105 647 231 354 391 987
Fragments arranged by size:
987 647 391 354 231 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 273 305 856 888 997 1029 2715
Size: 273 32 551 32 109 32 1686
Fragments arranged by size:
1686 551 273 109 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 616 2715
Size: 301 315 2099
Fragments arranged by size:
2099 315 301
XmaI C'CCGG_G
Cuts at: 0 28 2715
Size: 28 2687
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrGI BstXI BstYI BtgI BtsI CspCI EaeI EciI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NdeI NspI
PasI PfoI PmlI PpiI PspOMI PstI PvuII SacI
ScaI SfcI SfoI SmaI SmlI SpeI SrfI SspI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI BaeI BbvCI BfrBI BglII
BlpI BmtI Bpu10I BsaI BsiEI BsiWI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EarI
EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI NaeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SphI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI