Restriction map for
pFN21AE0018
(Linear) (Six-base) MAPSORT of: KIEE0018.seq Check: 2699 from: 1 to: 2568
KIEE0018.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1703 2568
Size: 1703 865
AccI GT'mk_AC
Cuts at: 0 281 2568
Size: 281 2287
AclI AA'CG_TT
Cuts at: 0 1004 1013 2568
Size: 1004 9 1555
Fragments arranged by size:
1555 1004 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 1810 2568
Size: 1795 15 758
Fragments arranged by size:
1795 758 15
AflIII A'CryG_T
Cuts at: 0 2450 2568
Size: 2450 118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 634 666 2568
Size: 634 32 1902
Fragments arranged by size:
1902 634 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1658 2568
Size: 1658 910
ApaI G_GGCC'C
Cuts at: 0 428 2568
Size: 428 2140
ApoI r'AATT_y
Cuts at: 0 232 692 1131 1239 1965 2346 2568
Size: 232 460 439 108 726 381 222
Fragments arranged by size:
726 460 439 381 232 222 108
AseI AT'TA_AT
Cuts at: 0 1733 2568
Size: 1733 835
AvaI C'yCGr_G
Cuts at: 0 1385 2568
Size: 1385 1183
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2291 2324 2568
Size: 2291 33 244
Fragments arranged by size:
2291 244 33
BanII G_rGCy'C
Cuts at: 0 428 2568
Size: 428 2140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1162 1407 1586 2568
Size: 1162 245 179 982
Fragments arranged by size:
1162 982 245 179
BciVI GTATCCnnnnn_n'
Cuts at: 0 1551 2568
Size: 1551 1017
BclI T'GATC_A
Cuts at: 0 1466 2568
Size: 1466 1102
BglII A'GATC_T
Cuts at: 0 657 2568
Size: 657 1911
BlpI GC'TnA_GC
Cuts at: 0 832 2568
Size: 832 1736
Bme1580I G_kGCm'C
Cuts at: 0 428 2568
Size: 428 2140
BmgBI CAC'GTC
Cuts at: 0 2451 2568
Size: 2451 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 618 1593 2568
Size: 618 975 975
Fragments arranged by size:
975 975 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 2568
Size: 581 1987
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 702 760 2568
Size: 118 584 58 1808
Fragments arranged by size:
1808 584 118 58
BsaHI Gr'CG_yC
Cuts at: 0 1412 2568
Size: 1412 1156
BsaWI w'CCGG_w
Cuts at: 0 1405 2568
Size: 1405 1163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 66 96 2437 2467 2568
Size: 66 30 2341 30 101
Fragments arranged by size:
2341 101 66 30 30
BseYI C'CCAG_C
Cuts at: 0 30 1278 2568
Size: 30 1248 1290
Fragments arranged by size:
1290 1248 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1826 2568
Size: 1826 742
BsiHKAI G_wGCw'C
Cuts at: 0 2053 2568
Size: 2053 515
BsmI GAATG_Cn'
Cuts at: 0 158 1195 2568
Size: 158 1037 1373
Fragments arranged by size:
1373 1037 158
Bsp1286I G_dGCh'C
Cuts at: 0 428 2053 2568
Size: 428 1625 515
Fragments arranged by size:
1625 515 428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 925 1102 1703 2568
Size: 925 177 601 865
Fragments arranged by size:
925 865 601 177
BsrBI CCG'CTC
Cuts at: 0 2293 2568
Size: 2293 275
BssSI C'ACGA_G
Cuts at: 0 1530 2568
Size: 1530 1038
BstXI CCAn_nnnn'nTGG
Cuts at: 0 145 2568
Size: 145 2423
BstYI r'GATC_y
Cuts at: 0 657 2568
Size: 657 1911
Bsu36I CC'TnA_GG
Cuts at: 0 86 433 2568
Size: 86 347 2135
Fragments arranged by size:
2135 347 86
BtgI C'CryG_G
Cuts at: 0 746 983 2568
Size: 746 237 1585
Fragments arranged by size:
1585 746 237
BtsI GCAGTG_nn'
Cuts at: 0 1764 2242 2568
Size: 1764 478 326
Fragments arranged by size:
1764 478 326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 372 407 2568
Size: 372 35 2161
Fragments arranged by size:
2161 372 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 277 2568
Size: 277 2291
EaeI y'GGCC_r
Cuts at: 0 2285 2568
Size: 2285 283
EarI CTCTTCn'nnn_
Cuts at: 0 799 1250 1694 2568
Size: 799 451 444 874
Fragments arranged by size:
874 799 451 444
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 704 2568
Size: 704 1864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1795 1810 2568
Size: 581 1214 15 758
Fragments arranged by size:
1214 758 581 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 432 2568
Size: 432 2136
EcoO109I rG'GnC_Cy
Cuts at: 0 425 2568
Size: 425 2143
EcoRI G'AATT_C
Cuts at: 0 692 1131 2568
Size: 692 439 1437
Fragments arranged by size:
1437 692 439
EcoRV GAT'ATC
Cuts at: 0 1149 2568
Size: 1149 1419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 701 727 733 759 1174 1206 1379 1411
Size: 701 26 6 26 415 32 173 32
Cuts at: 1411 2568
Size: 1157
Fragments arranged by size:
1157 701 415 173 32 32 26 26
6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 761 1068 1407 1413 1424 2081 2568
Size: 523 238 307 339 6 11 657 487
Fragments arranged by size:
657 523 487 339 307 238 11 6
MscI TGG'CCA
Cuts at: 0 2287 2568
Size: 2287 281
MslI CAynn'nnrTG
Cuts at: 0 1272 2525 2568
Size: 1272 1253 43
Fragments arranged by size:
1272 1253 43
MspA1I CmG'CkG
Cuts at: 0 948 2568
Size: 948 1620
NcoI C'CATG_G
Cuts at: 0 746 2568
Size: 746 1822
NdeI CA'TA_TG
Cuts at: 0 249 1926 2568
Size: 249 1677 642
Fragments arranged by size:
1677 642 249
NspI r_CATG'y
Cuts at: 0 1372 2568
Size: 1372 1196
PspOMI G'GGCC_C
Cuts at: 0 424 2568
Size: 424 2144
PstI C_TGCA'G
Cuts at: 0 1700 2237 2568
Size: 1700 537 331
Fragments arranged by size:
1700 537 331
PvuII CAG'CTG
Cuts at: 0 948 2568
Size: 948 1620
SapI GCTCTTCn'nnn_
Cuts at: 0 799 2568
Size: 799 1769
ScaI AGT'ACT
Cuts at: 0 2425 2568
Size: 2425 143
SexAI A'CCwGG_T
Cuts at: 0 515 2568
Size: 515 2053
SfcI C'TryA_G
Cuts at: 0 1696 2033 2233 2568
Size: 1696 337 200 335
Fragments arranged by size:
1696 337 335 200
SpeI A'CTAG_T
Cuts at: 0 171 2568
Size: 171 2397
SphI G_CATG'C
Cuts at: 0 1372 2568
Size: 1372 1196
StyI C'CwwG_G
Cuts at: 0 638 746 2568
Size: 638 108 1822
Fragments arranged by size:
1822 638 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 716 1270 1792 2568
Size: 716 554 522 776
Fragments arranged by size:
776 716 554 522
TatI w'GTAC_w
Cuts at: 0 626 823 1083 2423 2568
Size: 626 197 260 1340 145
Fragments arranged by size:
1340 626 260 197 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 66 98 2568
Size: 66 32 2470
Fragments arranged by size:
2470 66 32
XbaI T'CTAG_A
Cuts at: 0 1932 2295 2568
Size: 1932 363 273
Fragments arranged by size:
1932 363 273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 777 2484 2568
Size: 777 1707 84
Fragments arranged by size:
1707 777 84
XmnI GAAnn'nnTTC
Cuts at: 0 1705 2439 2568
Size: 1705 734 129
Fragments arranged by size:
1705 734 129
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AloI AlwNI ApaI
ApoI AseI AvaI BaeI BanII BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BssSI BstXI BstYI Bsu36I BtgI BtsI CspCI
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV Hin4I MmeI MscI MslI MspA1I NcoI NdeI
NspI PspOMI PstI PvuII SapI ScaI SexAI SfcI
SpeI SphI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI
ApaLI AscI AsiSI AvrII BamHI BanI BbeI BbvCI
BcgI BfrBI BglI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
ClaI DraI DraIII EagI EcoICRI FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SrfI SspI StuI SwaI Tth111I
XhoI XmaI ZraI