Restriction map for
pFN21AB9968
(Linear) (Six-base) MAPSORT of: KIBB9968.seq Check: 7699 from: 1 to: 2100
KIBB9968.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1238 2100
Size: 1238 862
AccI GT'mk_AC
Cuts at: 0 809 1695 2100
Size: 809 886 405
Fragments arranged by size:
886 809 405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 961 1984 2100
Size: 189 772 1023 116
Fragments arranged by size:
1023 772 189 116
AflIII A'CryG_T
Cuts at: 0 1151 1208 2100
Size: 1151 57 892
Fragments arranged by size:
1151 892 57
AleI CACnn'nnGTG
Cuts at: 0 1564 1889 2100
Size: 1564 325 211
Fragments arranged by size:
1564 325 211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 695 1907 2100
Size: 695 1212 193
Fragments arranged by size:
1212 695 193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 2100
Size: 1270 32 798
Fragments arranged by size:
1270 798 32
AlwNI CAG_nnn'CTG
Cuts at: 0 138 2100
Size: 138 1962
ApaI G_GGCC'C
Cuts at: 0 326 989 2100
Size: 326 663 1111
Fragments arranged by size:
1111 663 326
ApaLI G'TGCA_C
Cuts at: 0 976 1882 2100
Size: 976 906 218
Fragments arranged by size:
976 906 218
ApoI r'AATT_y
Cuts at: 0 548 607 1093 2038 2100
Size: 548 59 486 945 62
Fragments arranged by size:
945 548 486 62 59
AvaI C'yCGr_G
Cuts at: 0 120 1174 2100
Size: 120 1054 926
Fragments arranged by size:
1054 926 120
BanI G'GyrC_C
Cuts at: 0 124 1027 1246 2100
Size: 124 903 219 854
Fragments arranged by size:
903 854 219 124
BanII G_rGCy'C
Cuts at: 0 8 57 326 989 1633 2100
Size: 8 49 269 663 644 467
Fragments arranged by size:
663 644 467 269 49 8
BbeI G_GCGC'C
Cuts at: 0 128 2100
Size: 128 1972
BbvCI CC'TCA_GC
Cuts at: 0 1927 2100
Size: 1927 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 58 92 2100
Size: 58 34 2008
Fragments arranged by size:
2008 58 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1025 1054 2100
Size: 1025 29 1046
Fragments arranged by size:
1046 1025 29
BlpI GC'TnA_GC
Cuts at: 0 304 2100
Size: 304 1796
Bme1580I G_kGCm'C
Cuts at: 0 326 980 989 1030 1886 2100
Size: 326 654 9 41 856 214
Fragments arranged by size:
856 654 326 214 41 9
BmgBI CAC'GTC
Cuts at: 0 1209 2100
Size: 1209 891
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 435 2100
Size: 435 1665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 675 1240 1297 1551 2100
Size: 379 296 565 57 254 549
Fragments arranged by size:
565 549 379 296 254 57
Bpu10I CC'TnA_GC
Cuts at: 0 1927 2100
Size: 1927 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 934 2100
Size: 605 329 1166
Fragments arranged by size:
1166 605 329
BsaI GGTCTCn'nnnn_
Cuts at: 0 64 882 1998 2100
Size: 64 818 1116 102
Fragments arranged by size:
1116 818 102 64
BsaBI GATnn'nnATC
Cuts at: 0 373 2100
Size: 373 1727
BsaHI Gr'CG_yC
Cuts at: 0 125 2100
Size: 125 1975
BsaWI w'CCGG_w
Cuts at: 0 114 821 1307 2100
Size: 114 707 486 793
Fragments arranged by size:
793 707 486 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 661 691 1198 1228 1270 1300 2100
Size: 25 636 30 507 30 42 30 800
Fragments arranged by size:
800 636 507 42 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 163 166 289 376 379 424 1294
Size: 160 3 3 123 87 3 45 870
Cuts at: 1294 1297 1756 2100
Size: 3 459 344
Fragments arranged by size:
870 459 344 160 123 87 45 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 131 982 1900 2100
Size: 131 851 918 200
Fragments arranged by size:
918 851 200 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1603 1861 2100
Size: 871 732 258 239
Fragments arranged by size:
871 732 258 239
BsiHKAI G_wGCw'C
Cuts at: 0 185 356 980 1886 2100
Size: 185 171 624 906 214
Fragments arranged by size:
906 624 214 185 171
Bsp1286I G_dGCh'C
Cuts at: 0 8 57 185 326 356 980 989 1030
Size: 8 49 128 141 30 624 9 41
Cuts at: 1030 1633 1886 2100
Size: 603 253 214
Fragments arranged by size:
624 603 253 214 141 128 49 41
30 9 8
BspEI T'CCGG_A
Cuts at: 0 114 821 1307 2100
Size: 114 707 486 793
Fragments arranged by size:
793 707 486 114
BspHI T'CATG_A
Cuts at: 0 1313 2100
Size: 1313 787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 1238 1737 2100
Size: 299 939 499 363
Fragments arranged by size:
939 499 363 299
BsrBI CCG'CTC
Cuts at: 0 45 2100
Size: 45 2055
BsrDI GCAATG_nn'
Cuts at: 0 421 2100
Size: 421 1679
BsrFI r'CCGG_y
Cuts at: 0 1344 2100
Size: 1344 756
BssSI C'ACGA_G
Cuts at: 0 178 2100
Size: 178 1922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1239 2100
Size: 1239 861
BstEII G'GTnAC_C
Cuts at: 0 311 2100
Size: 311 1789
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 544 2100
Size: 348 196 1556
Fragments arranged by size:
1556 348 196
BstYI r'GATC_y
Cuts at: 0 368 379 2100
Size: 368 11 1721
Fragments arranged by size:
1721 368 11
BstZ17I GTA'TAC
Cuts at: 0 1696 2100
Size: 1696 404
Bsu36I CC'TnA_GG
Cuts at: 0 105 318 2100
Size: 105 213 1782
Fragments arranged by size:
1782 213 105
BtgI C'CryG_G
Cuts at: 0 347 1776 2100
Size: 347 1429 324
Fragments arranged by size:
1429 347 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1473 1815 2100
Size: 1473 342 285
Fragments arranged by size:
1473 342 285
BtsI GCAGTG_nn'
Cuts at: 0 74 1884 1896 2100
Size: 74 1810 12 204
Fragments arranged by size:
1810 204 74 12
ClaI AT'CG_AT
Cuts at: 0 1043 2100
Size: 1043 1057
DrdI GACnn_nn'nnGTC
Cuts at: 0 1708 2100
Size: 1708 392
EaeI y'GGCC_r
Cuts at: 0 1055 1618 2100
Size: 1055 563 482
Fragments arranged by size:
1055 563 482
EarI CTCTTCn'nnn_
Cuts at: 0 1981 2100
Size: 1981 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 372 2100
Size: 372 1728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 379 675 961 1240 1297 1551 1984
Size: 189 190 296 286 279 57 254 433
Cuts at: 1984 2100
Size: 116
Fragments arranged by size:
433 296 286 279 254 190 189 116
57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 2100
Size: 361 1739
EcoO109I rG'GnC_Cy
Cuts at: 0 109 322 625 2100
Size: 109 213 303 1475
Fragments arranged by size:
1475 303 213 109
EcoRI G'AATT_C
Cuts at: 0 548 2038 2100
Size: 548 1490 62
Fragments arranged by size:
1490 548 62
EcoRV GAT'ATC
Cuts at: 0 493 2100
Size: 493 1607
HaeII r_GCGC'y
Cuts at: 0 128 1122 2100
Size: 128 994 978
Fragments arranged by size:
994 978 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 661 693 1198 1230 2100
Size: 661 32 505 32 870
Fragments arranged by size:
870 661 505 32 32
HincII GTy'rAC
Cuts at: 0 645 698 810 2100
Size: 645 53 112 1290
Fragments arranged by size:
1290 645 112 53
HpaI GTT'AAC
Cuts at: 0 645 698 2100
Size: 645 53 1402
Fragments arranged by size:
1402 645 53
KasI G'GCGC_C
Cuts at: 0 124 2100
Size: 124 1976
MscI TGG'CCA
Cuts at: 0 1057 1620 2100
Size: 1057 563 480
Fragments arranged by size:
1057 563 480
MslI CAynn'nnrTG
Cuts at: 0 346 691 778 934 1564 1879 1889 2100
Size: 346 345 87 156 630 315 10 211
Fragments arranged by size:
630 346 345 315 211 156 87 10
MspA1I CmG'CkG
Cuts at: 0 138 162 885 899 2100
Size: 138 24 723 14 1201
Fragments arranged by size:
1201 723 138 24 14
NaeI GCC'GGC
Cuts at: 0 1346 2100
Size: 1346 754
NarI GG'CG_CC
Cuts at: 0 125 2100
Size: 125 1975
NdeI CA'TA_TG
Cuts at: 0 2012 2100
Size: 2012 88
NgoMIV G'CCGG_C
Cuts at: 0 1344 2100
Size: 1344 756
PasI CC'CwG_GG
Cuts at: 0 1497 2100
Size: 1497 603
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 2100
Size: 1270 32 798
Fragments arranged by size:
1270 798 32
PpuMI rG'GwC_Cy
Cuts at: 0 625 2100
Size: 625 1475
PshAI GACnn'nnGTC
Cuts at: 0 688 1303 2100
Size: 688 615 797
Fragments arranged by size:
797 688 615
PspOMI G'GGCC_C
Cuts at: 0 322 985 2100
Size: 322 663 1115
Fragments arranged by size:
1115 663 322
PspXI vC'TCGA_Gb
Cuts at: 0 1174 2100
Size: 1174 926
PstI C_TGCA'G
Cuts at: 0 1235 1891 2100
Size: 1235 656 209
Fragments arranged by size:
1235 656 209
PvuII CAG'CTG
Cuts at: 0 885 899 2100
Size: 885 14 1201
Fragments arranged by size:
1201 885 14
SalI G'TCGA_C
Cuts at: 0 808 2100
Size: 808 1292
ScaI AGT'ACT
Cuts at: 0 739 1180 2100
Size: 739 441 920
Fragments arranged by size:
920 739 441
SfcI C'TryA_G
Cuts at: 0 1002 1231 1887 2100
Size: 1002 229 656 213
Fragments arranged by size:
1002 656 229 213
SfoI GGC'GCC
Cuts at: 0 126 2100
Size: 126 1974
SmaI CCC'GGG
Cuts at: 0 122 2100
Size: 122 1978
SmlI C'TyrA_G
Cuts at: 0 620 949 1174 2100
Size: 620 329 225 926
Fragments arranged by size:
926 620 329 225
StyI C'CwwG_G
Cuts at: 0 750 2100
Size: 750 1350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1270 1510 2100
Size: 1270 240 590
Fragments arranged by size:
1270 590 240
TatI w'GTAC_w
Cuts at: 0 737 1148 1178 2100
Size: 737 411 30 922
Fragments arranged by size:
922 737 411 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 2100
Size: 27 2073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 2100
Size: 267 1833
XhoI C'TCGA_G
Cuts at: 0 1174 2100
Size: 1174 926
XmaI C'CCGG_G
Cuts at: 0 120 2100
Size: 120 1980
XmnI GAAnn'nnTTC
Cuts at: 0 607 2100
Size: 607 1493
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbvCI
BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
HpaI KasI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV PasI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SalI ScaI SfcI SfoI SmaI SmlI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI
BfrBI BglII BmrI BmtI BsaAI BsiEI BsiWI BsmI
BsmBI BsrGI BssHII BstBI CspCI DraI DraIII EagI
EcoICRI FalI FseI FspI FspAI HindIII KpnI MfeI
MluI MmeI NcoI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PsiI PsrI
PvuI RsrII SacI SacII SanDI SapI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI ZraI