Restriction map for
pFN21AB9956
(Linear) (Six-base) MAPSORT of: KIBB9956.seq Check: 4838 from: 1 to: 2052
KIBB9956.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 578 1037 1051 1648 1736 2052
Size: 395 183 459 14 597 88 316
Fragments arranged by size:
597 459 395 316 183 88 14
AflIII A'CryG_T
Cuts at: 0 1208 2052
Size: 1208 844
AleI CACnn'nnGTG
Cuts at: 0 1328 2052
Size: 1328 724
ApoI r'AATT_y
Cuts at: 0 18 513 529 1728 1783 1857 2021 2052
Size: 18 495 16 1199 55 74 164 31
Fragments arranged by size:
1199 495 164 74 55 31 18 16
AvrII C'CTAG_G
Cuts at: 0 804 2052
Size: 804 1248
BanI G'GyrC_C
Cuts at: 0 135 2052
Size: 135 1917
BanII G_rGCy'C
Cuts at: 0 40 298 1274 2052
Size: 40 258 976 778
Fragments arranged by size:
976 778 258 40
BbvCI CC'TCA_GC
Cuts at: 0 1928 2052
Size: 1928 124
BciVI GTATCCnnnnn_n'
Cuts at: 0 1145 1266 2052
Size: 1145 121 786
Fragments arranged by size:
1145 786 121
BfrBI ATG'CAT
Cuts at: 0 915 1432 2052
Size: 915 517 620
Fragments arranged by size:
915 620 517
BglI GCCn_nnn'nGGC
Cuts at: 0 953 2052
Size: 953 1099
BmrI ACTGGGnnnn_n'
Cuts at: 0 245 631 753 828 960 1254 2052
Size: 245 386 122 75 132 294 798
Fragments arranged by size:
798 386 294 245 132 122 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 895 2052
Size: 523 372 1157
Fragments arranged by size:
1157 523 372
Bpu10I CC'TnA_GC
Cuts at: 0 1928 2052
Size: 1928 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 2052
Size: 1393 659
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 188 218 2052
Size: 188 30 1834
Fragments arranged by size:
1834 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 494 2052
Size: 494 1558
BseYI C'CCAG_C
Cuts at: 0 81 300 1192 2052
Size: 81 219 892 860
Fragments arranged by size:
892 860 219 81
BsiHKAI G_wGCw'C
Cuts at: 0 298 1274 2052
Size: 298 976 778
Fragments arranged by size:
976 778 298
BsmI GAATG_Cn'
Cuts at: 0 330 805 915 2052
Size: 330 475 110 1137
Fragments arranged by size:
1137 475 330 110
Bsp1286I G_dGCh'C
Cuts at: 0 40 298 1274 2052
Size: 40 258 976 778
Fragments arranged by size:
976 778 258 40
BsrDI GCAATG_nn'
Cuts at: 0 397 975 2052
Size: 397 578 1077
Fragments arranged by size:
1077 578 397
BsrGI T'GTAC_A
Cuts at: 0 569 2052
Size: 569 1483
BstAPI GCAn_nnn'nTGC
Cuts at: 0 322 2052
Size: 322 1730
BstEII G'GTnAC_C
Cuts at: 0 1156 2052
Size: 1156 896
Bsu36I CC'TnA_GG
Cuts at: 0 380 2052
Size: 380 1672
BtsI GCAGTG_nn'
Cuts at: 0 783 2052
Size: 783 1269
DraI TTT'AAA
Cuts at: 0 245 1397 1616 2052
Size: 245 1152 219 436
Fragments arranged by size:
1152 436 245 219
EaeI y'GGCC_r
Cuts at: 0 703 2052
Size: 703 1349
EarI CTCTTCn'nnn_
Cuts at: 0 374 2052
Size: 374 1678
EcoICRI GAG'CTC
Cuts at: 0 296 1272 2052
Size: 296 976 780
Fragments arranged by size:
976 780 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 523 578 895 1037 1051 1648 1736
Size: 395 128 55 317 142 14 597 88
Cuts at: 1736 2052
Size: 316
Fragments arranged by size:
597 395 317 316 142 128 88 55
14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 608 2052
Size: 174 434 1444
Fragments arranged by size:
1444 434 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 903 935 1093 1125 1732 1764 2052
Size: 903 32 158 32 607 32 288
Fragments arranged by size:
903 607 288 158 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 456 2052
Size: 425 31 1596
Fragments arranged by size:
1596 425 31
MslI CAynn'nnrTG
Cuts at: 0 113 161 1328 2052
Size: 113 48 1167 724
Fragments arranged by size:
1167 724 113 48
NsiI A_TGCA'T
Cuts at: 0 917 1434 2052
Size: 917 517 618
Fragments arranged by size:
917 618 517
NspI r_CATG'y
Cuts at: 0 205 348 1212 1768 2052
Size: 205 143 864 556 284
Fragments arranged by size:
864 556 284 205 143
PciI A'CATG_T
Cuts at: 0 1208 2052
Size: 1208 844
PflMI CCAn_nnn'nTGG
Cuts at: 0 652 2052
Size: 652 1400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 702 734 2052
Size: 702 32 1318
Fragments arranged by size:
1318 702 32
PsiI TTA'TAA
Cuts at: 0 1144 2052
Size: 1144 908
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 985 1017 2052
Size: 985 32 1035
Fragments arranged by size:
1035 985 32
SacI G_AGCT'C
Cuts at: 0 298 1274 2052
Size: 298 976 778
Fragments arranged by size:
976 778 298
SmlI C'TyrA_G
Cuts at: 0 1372 2052
Size: 1372 680
StyI C'CwwG_G
Cuts at: 0 26 804 2052
Size: 26 778 1248
Fragments arranged by size:
1248 778 26
SwaI ATTT'AAAT
Cuts at: 0 245 2052
Size: 245 1807
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 2052
Size: 292 1760
TatI w'GTAC_w
Cuts at: 0 217 569 589 2052
Size: 217 352 20 1463
Fragments arranged by size:
1463 352 217 20
Enzymes that do cut:
AcuI AflIII AleI ApoI AvrII BanI BanII BbvCI
BciVI BfrBI BglI BmrI BpmI Bpu10I BpuEI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrGI BstAPI
BstEII Bsu36I BtsI DraI EaeI EarI EcoICRI Eco57MI
FalI Hin4I MmeI MslI NsiI NspI PciI PflMI
PpiI PsiI PsrI SacI SmlI StyI SwaI TaqII
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BbeI BbsI BcgI BclI
BglII BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstXI
BstYI BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI