(Linear) (Six-base) MAPSORT of: KIBB9945.seq  Check: 7876  from: 1  to: 1590



KIBB9945.orf

 With 182 enzymes: SgfI * 

                        April 1, 2011 16:20  ..

AccI GT'mk_AC

Cuts at:      0      74    1246    1590 
   Size:         74    1172     344

  Fragments arranged by size: 

                1172     344      74
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     599     656     845    1132    1588    1590 
   Size:        599      57     189     287     456       2

  Fragments arranged by size: 

                 599     456     287     189      57       2
 
AflIII A'CryG_T

Cuts at:      0    1274    1590 
   Size:       1274     316
 
AleI CACnn'nnGTG

Cuts at:      0    1312    1590 
   Size:       1312     278
 
AlwNI CAG_nnn'CTG

Cuts at:      0     511    1590 
   Size:        511    1079
 
ApoI r'AATT_y

Cuts at:      0     271     786    1080    1590 
   Size:        271     515     294     510

  Fragments arranged by size: 

                 515     510     294     271
 
AvaI C'yCGr_G

Cuts at:      0      68    1590 
   Size:         68    1522
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1499    1590 
   Size:       1499      91
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1055    1590 
   Size:       1055     535
 
BclI T'GATC_A

Cuts at:      0     840    1304    1590 
   Size:        840     464     286

  Fragments arranged by size: 

                 840     464     286
 
BfrBI ATG'CAT

Cuts at:      0     310    1118    1590 
   Size:        310     808     472

  Fragments arranged by size: 

                 808     472     310
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41    1590 
   Size:         41    1549
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1436    1590 
   Size:       1436     154
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      17    1590 
   Size:         17    1573
 
BsaBI GATnn'nnATC

Cuts at:      0     257    1460    1590 
   Size:        257    1203     130

  Fragments arranged by size: 

                1203     257     130
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1286    1316    1590 
   Size:       1286      30     274

  Fragments arranged by size: 

                1286     274      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     493    1590 
   Size:        493    1097
 
BspHI T'CATG_A

Cuts at:      0     141    1590 
   Size:        141    1449
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     555    1590 
   Size:        555    1035
 
BsrDI GCAATG_nn'

Cuts at:      0     393    1494    1590 
   Size:        393    1101      96

  Fragments arranged by size: 

                1101     393      96
 
BstYI r'GATC_y

Cuts at:      0    1441    1590 
   Size:       1441     149
 
EaeI y'GGCC_r

Cuts at:      0      61    1590 
   Size:         61    1529
 
EarI CTCTTCn'nnn_

Cuts at:      0     632     698     853    1538    1590 
   Size:        632      66     155     685      52

  Fragments arranged by size: 

                 685     632     155      66      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     599     656     845    1132    1588    1590 
   Size:         41     558      57     189     287     456       2

  Fragments arranged by size: 

                 558     456     287     189      57      41       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     536    1590 
   Size:        536    1054
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      14      46    1590 
   Size:         14      32    1544

  Fragments arranged by size: 

                1544      32      14
 
HincII GTy'rAC

Cuts at:      0     283     411    1590 
   Size:        283     128    1179

  Fragments arranged by size: 

                1179     283     128
 
MscI TGG'CCA

Cuts at:      0      63    1590 
   Size:         63    1527
 
MslI CAynn'nnrTG

Cuts at:      0    1183    1312    1590 
   Size:       1183     129     278

  Fragments arranged by size: 

                1183     278     129
 
NruI TCG'CGA

Cuts at:      0       8    1590 
   Size:          8    1582
 
NsiI A_TGCA'T

Cuts at:      0     312    1120    1590 
   Size:        312     808     470

  Fragments arranged by size: 

                 808     470     312
 
NspI r_CATG'y

Cuts at:      0    1069    1278    1590 
   Size:       1069     209     312

  Fragments arranged by size: 

                1069     312     209
 
PciI A'CATG_T

Cuts at:      0    1274    1590 
   Size:       1274     316
 
PspXI vC'TCGA_Gb

Cuts at:      0      68    1590 
   Size:         68    1522
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      65      97    1590 
   Size:         65      32    1493

  Fragments arranged by size: 

                1493      65      32
 
PstI C_TGCA'G

Cuts at:      0     552    1590 
   Size:        552    1038
 
SfcI C'TryA_G

Cuts at:      0     548     554    1590 
   Size:        548       6    1036

  Fragments arranged by size: 

                1036     548       6
 
SmlI C'TyrA_G

Cuts at:      0      68    1451    1590 
   Size:         68    1383     139

  Fragments arranged by size: 

                1383     139      68
 
SpeI A'CTAG_T

Cuts at:      0     343    1590 
   Size:        343    1247
 
StuI AGG'CCT

Cuts at:      0    1126    1590 
   Size:       1126     464
 
StyI C'CwwG_G

Cuts at:      0    1127    1590 
   Size:       1127     463
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     120    1324    1590 
   Size:        120    1204     266

  Fragments arranged by size: 

                1204     266     120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1286    1318    1590 
   Size:       1286      32     272

  Fragments arranged by size: 

                1286     272      32
 
XhoI C'TCGA_G

Cuts at:      0      68    1590 
   Size:         68    1522
 
XmnI GAAnn'nnTTC

Cuts at:      0    1276    1590 
   Size:       1276     314
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI     ApoI     AvaI     BbsI
    BciVI     BclI    BfrBI     BpmI    BpuEI     BsaI    BsaBI    BsaXI
    BseRI    BspHI    BspMI    BsrDI    BstYI     EaeI     EarI  Eco57MI
    EcoNI    Hin4I   HincII     MscI     MslI     NruI     NsiI     NspI
     PciI    PspXI     PsrI     PstI     SfcI     SmlI     SpeI     StuI
     StyI    TaqII     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XcmI     XmaI     ZraI