Restriction map for
pFN21AB9932
(Linear) (Six-base) MAPSORT of: KIBB9932.seq Check: 9593 from: 1 to: 1914
KIBB9932.orf
With 182 enzymes: SgfI *
July 3, 2012 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1203 1215 1914
Size: 1203 12 699
Fragments arranged by size:
1203 699 12
AccI GT'mk_AC
Cuts at: 0 1829 1914
Size: 1829 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 592 1914
Size: 75 517 1322
Fragments arranged by size:
1322 517 75
AflII C'TTAA_G
Cuts at: 0 1044 1914
Size: 1044 870
AleI CACnn'nnGTG
Cuts at: 0 1253 1914
Size: 1253 661
AlwNI CAG_nnn'CTG
Cuts at: 0 289 1914
Size: 289 1625
ApaLI G'TGCA_C
Cuts at: 0 150 420 1914
Size: 150 270 1494
Fragments arranged by size:
1494 270 150
ApoI r'AATT_y
Cuts at: 0 884 1489 1914
Size: 884 605 425
Fragments arranged by size:
884 605 425
AvaI C'yCGr_G
Cuts at: 0 891 1914
Size: 891 1023
BanI G'GyrC_C
Cuts at: 0 1247 1914
Size: 1247 667
BanII G_rGCy'C
Cuts at: 0 1102 1914
Size: 1102 812
BbsI GAAGACnn'nnnn_
Cuts at: 0 760 1019 1914
Size: 760 259 895
Fragments arranged by size:
895 760 259
Bme1580I G_kGCm'C
Cuts at: 0 154 424 585 1809 1914
Size: 154 270 161 1224 105
Fragments arranged by size:
1224 270 161 154 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 1139 1914
Size: 1139 775
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1282 1914
Size: 1282 632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1450 1914
Size: 647 803 464
Fragments arranged by size:
803 647 464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1914
Size: 797 1117
BsaWI w'CCGG_w
Cuts at: 0 485 1558 1914
Size: 485 1073 356
Fragments arranged by size:
1073 485 356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 932 962 1089 1119 1914
Size: 205 30 697 30 127 30 795
Fragments arranged by size:
795 697 205 127 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 380 800 923 1914
Size: 380 420 123 991
Fragments arranged by size:
991 420 380 123
BseYI C'CCAG_C
Cuts at: 0 1790 1914
Size: 1790 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 675 1131 1914
Size: 302 373 456 783
Fragments arranged by size:
783 456 373 302
BsiHKAI G_wGCw'C
Cuts at: 0 154 424 1102 1914
Size: 154 270 678 812
Fragments arranged by size:
812 678 270 154
BsmI GAATG_Cn'
Cuts at: 0 412 1645 1914
Size: 412 1233 269
Fragments arranged by size:
1233 412 269
Bsp1286I G_dGCh'C
Cuts at: 0 154 424 585 1102 1809 1914
Size: 154 270 161 517 707 105
Fragments arranged by size:
707 517 270 161 154 105
BspHI T'CATG_A
Cuts at: 0 1443 1914
Size: 1443 471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 856 1203 1215 1914
Size: 856 347 12 699
Fragments arranged by size:
856 699 347 12
BsrDI GCAATG_nn'
Cuts at: 0 313 1914
Size: 313 1601
BsrGI T'GTAC_A
Cuts at: 0 116 1914
Size: 116 1798
BstEII G'GTnAC_C
Cuts at: 0 1554 1914
Size: 1554 360
BtsI GCAGTG_nn'
Cuts at: 0 402 1289 1299 1887 1914
Size: 402 887 10 588 27
Fragments arranged by size:
887 588 402 27 10
DraI TTT'AAA
Cuts at: 0 1355 1914
Size: 1355 559
DrdI GACnn_nn'nnGTC
Cuts at: 0 1141 1914
Size: 1141 773
EarI CTCTTCn'nnn_
Cuts at: 0 718 1019 1194 1914
Size: 718 301 175 720
Fragments arranged by size:
720 718 301 175
EcoICRI GAG'CTC
Cuts at: 0 1100 1914
Size: 1100 814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 592 647 1450 1914
Size: 75 517 55 803 464
Fragments arranged by size:
803 517 464 75 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1517 1914
Size: 1517 397
EcoO109I rG'GnC_Cy
Cuts at: 0 1284 1914
Size: 1284 630
EcoRI G'AATT_C
Cuts at: 0 1489 1914
Size: 1489 425
EcoRV GAT'ATC
Cuts at: 0 1449 1654 1914
Size: 1449 205 260
Fragments arranged by size:
1449 260 205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1059 1914
Size: 1059 855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 966 998 1914
Size: 966 32 916
Fragments arranged by size:
966 916 32
HincII GTy'rAC
Cuts at: 0 900 1914
Size: 900 1014
MfeI C'AATT_G
Cuts at: 0 492 1914
Size: 492 1422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1093 1155 1914
Size: 661 432 62 759
Fragments arranged by size:
759 661 432 62
MslI CAynn'nnrTG
Cuts at: 0 970 1253 1914
Size: 970 283 661
Fragments arranged by size:
970 661 283
MspA1I CmG'CkG
Cuts at: 0 1790 1914
Size: 1790 124
NdeI CA'TA_TG
Cuts at: 0 453 1914
Size: 453 1461
PflMI CCAn_nnn'nTGG
Cuts at: 0 1550 1680 1914
Size: 1550 130 234
Fragments arranged by size:
1550 234 130
PfoI T'CCnGG_A
Cuts at: 0 513 1914
Size: 513 1401
PpuMI rG'GwC_Cy
Cuts at: 0 1284 1914
Size: 1284 630
PsiI TTA'TAA
Cuts at: 0 347 1664 1914
Size: 347 1317 250
Fragments arranged by size:
1317 347 250
PstI C_TGCA'G
Cuts at: 0 1226 1325 1914
Size: 1226 99 589
Fragments arranged by size:
1226 589 99
PvuII CAG'CTG
Cuts at: 0 1790 1914
Size: 1790 124
SacI G_AGCT'C
Cuts at: 0 1102 1914
Size: 1102 812
SbfI CC_TGCA'GG
Cuts at: 0 1226 1325 1914
Size: 1226 99 589
Fragments arranged by size:
1226 589 99
SexAI A'CCwGG_T
Cuts at: 0 1821 1914
Size: 1821 93
SfcI C'TryA_G
Cuts at: 0 44 1222 1321 1341 1914
Size: 44 1178 99 20 573
Fragments arranged by size:
1178 573 99 44 20
SmaI CCC'GGG
Cuts at: 0 893 1914
Size: 893 1021
SmlI C'TyrA_G
Cuts at: 0 812 1044 1914
Size: 812 232 870
Fragments arranged by size:
870 812 232
SspI AAT'ATT
Cuts at: 0 1599 1617 1914
Size: 1599 18 297
Fragments arranged by size:
1599 297 18
StyI C'CwwG_G
Cuts at: 0 163 1914
Size: 163 1751
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 176 1914
Size: 176 1738
TatI w'GTAC_w
Cuts at: 0 116 267 506 1847 1914
Size: 116 151 239 1341 67
Fragments arranged by size:
1341 239 151 116 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 1671 1703 1914
Size: 1015 32 624 32 211
Fragments arranged by size:
1015 624 211 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1306 1914
Size: 1306 608
XmaI C'CCGG_G
Cuts at: 0 891 1914
Size: 891 1023
XmnI GAAnn'nnTTC
Cuts at: 0 613 1914
Size: 613 1301
Enzymes that do cut:
AarI AccI AcuI AflII AleI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI Bme1580I BmrI BplI BpmI
BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrGI BstEII BtsI DraI
DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII MfeI MmeI MslI MspA1I NdeI
PflMI PfoI PpuMI PsiI PstI PvuII SacI SbfI
SexAI SfcI SmaI SmlI SspI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII EaeI EagI EciI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XhoI ZraI