Restriction map for
pFN21AB9930
(Linear) (Six-base) MAPSORT of: KIBB9930.seq Check: 3930 from: 1 to: 1701
KIBB9930.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
Acc65I G'GTAC_C
Cuts at: 0 764 1701
Size: 764 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 544 1480 1701
Size: 532 12 936 221
Fragments arranged by size:
936 532 221 12
AflIII A'CryG_T
Cuts at: 0 1046 1701
Size: 1046 655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 707 1701
Size: 707 994
AlwNI CAG_nnn'CTG
Cuts at: 0 510 1701
Size: 510 1191
ApoI r'AATT_y
Cuts at: 0 75 1701
Size: 75 1626
BanI G'GyrC_C
Cuts at: 0 764 1701
Size: 764 937
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 702 1701
Size: 34 668 999
Fragments arranged by size:
999 668 34
BbvCI CC'TCA_GC
Cuts at: 0 1363 1701
Size: 1363 338
BglI GCCn_nnn'nGGC
Cuts at: 0 827 1144 1701
Size: 827 317 557
Fragments arranged by size:
827 557 317
BlpI GC'TnA_GC
Cuts at: 0 895 1603 1701
Size: 895 708 98
Fragments arranged by size:
895 708 98
Bme1580I G_kGCm'C
Cuts at: 0 1064 1701
Size: 1064 637
BmgBI CAC'GTC
Cuts at: 0 1562 1623 1701
Size: 1562 61 78
Fragments arranged by size:
1562 78 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 651 1701
Size: 651 1050
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 885 1317 1701
Size: 885 432 384
Fragments arranged by size:
885 432 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1405 1701
Size: 1076 329 296
Fragments arranged by size:
1076 329 296
Bpu10I CC'TnA_GC
Cuts at: 0 962 1363 1487 1701
Size: 962 401 124 214
Fragments arranged by size:
962 401 214 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1206 1701
Size: 1206 495
BsaWI w'CCGG_w
Cuts at: 0 761 1701
Size: 761 940
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1158 1188 1701
Size: 1158 30 513
Fragments arranged by size:
1158 513 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 319 322 641 995 1701
Size: 246 73 3 319 354 706
Fragments arranged by size:
706 354 319 246 73 3
BseYI C'CCAG_C
Cuts at: 0 1304 1701
Size: 1304 397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1645 1676 1701
Size: 1645 31 25
Fragments arranged by size:
1645 31 25
BsiEI CG_ry'CG
Cuts at: 0 1533 1701
Size: 1533 168
BsiHKAI G_wGCw'C
Cuts at: 0 72 575 730 1701
Size: 72 503 155 971
Fragments arranged by size:
971 503 155 72
BsmI GAATG_Cn'
Cuts at: 0 864 1701
Size: 864 837
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1282 1613 1701
Size: 1282 331 88
Fragments arranged by size:
1282 331 88
Bsp1286I G_dGCh'C
Cuts at: 0 72 575 730 1064 1701
Size: 72 503 155 334 637
Fragments arranged by size:
637 503 334 155 72
BsrBI CCG'CTC
Cuts at: 0 1607 1701
Size: 1607 94
BsrFI r'CCGG_y
Cuts at: 0 826 1532 1567 1701
Size: 826 706 35 134
Fragments arranged by size:
826 706 134 35
BsrGI T'GTAC_A
Cuts at: 0 129 1701
Size: 129 1572
BssSI C'ACGA_G
Cuts at: 0 933 1291 1701
Size: 933 358 410
Fragments arranged by size:
933 410 358
BstEII G'GTnAC_C
Cuts at: 0 1499 1701
Size: 1499 202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1344 1701
Size: 1344 357
BstYI r'GATC_y
Cuts at: 0 1256 1701
Size: 1256 445
BtgI C'CryG_G
Cuts at: 0 32 719 1034 1521 1701
Size: 32 687 315 487 180
Fragments arranged by size:
687 487 315 180 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1496 1701
Size: 1496 205
BtsI GCAGTG_nn'
Cuts at: 0 784 1701
Size: 784 917
EaeI y'GGCC_r
Cuts at: 0 819 1530 1534 1701
Size: 819 711 4 167
Fragments arranged by size:
819 711 167 4
EagI C'GGCC_G
Cuts at: 0 1530 1701
Size: 1530 171
EarI CTCTTCn'nnn_
Cuts at: 0 37 1436 1463 1664 1701
Size: 37 1399 27 201 37
Fragments arranged by size:
1399 201 37 37 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1270 1363 1701
Size: 1270 93 338
Fragments arranged by size:
1270 338 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 544 1076 1405 1480 1701
Size: 532 12 532 329 75 221
Fragments arranged by size:
532 532 329 221 75 12
EcoRV GAT'ATC
Cuts at: 0 162 1701
Size: 162 1539
FseI GG_CCGG'CC
Cuts at: 0 1536 1701
Size: 1536 165
HaeII r_GCGC'y
Cuts at: 0 285 1701
Size: 285 1416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1158 1190 1260 1292 1701
Size: 1158 32 70 32 409
Fragments arranged by size:
1158 409 70 32 32
HincII GTy'rAC
Cuts at: 0 380 1701
Size: 380 1321
KpnI G_GTAC'C
Cuts at: 0 768 1701
Size: 768 933
MscI TGG'CCA
Cuts at: 0 821 1701
Size: 821 880
MslI CAynn'nnrTG
Cuts at: 0 61 637 1549 1701
Size: 61 576 912 152
Fragments arranged by size:
912 576 152 61
MspA1I CmG'CkG
Cuts at: 0 51 538 1437 1701
Size: 51 487 899 264
Fragments arranged by size:
899 487 264 51
NaeI GCC'GGC
Cuts at: 0 828 1534 1569 1701
Size: 828 706 35 132
Fragments arranged by size:
828 706 132 35
NcoI C'CATG_G
Cuts at: 0 32 1034 1521 1701
Size: 32 1002 487 180
Fragments arranged by size:
1002 487 180 32
NdeI CA'TA_TG
Cuts at: 0 354 1701
Size: 354 1347
NgoMIV G'CCGG_C
Cuts at: 0 826 1532 1567 1701
Size: 826 706 35 134
Fragments arranged by size:
826 706 134 35
NspI r_CATG'y
Cuts at: 0 1050 1701
Size: 1050 651
PciI A'CATG_T
Cuts at: 0 1046 1701
Size: 1046 655
PflMI CCAn_nnn'nTGG
Cuts at: 0 559 720 1701
Size: 559 161 981
Fragments arranged by size:
981 559 161
PstI C_TGCA'G
Cuts at: 0 1152 1265 1409 1701
Size: 1152 113 144 292
Fragments arranged by size:
1152 292 144 113
PvuII CAG'CTG
Cuts at: 0 51 538 1437 1701
Size: 51 487 899 264
Fragments arranged by size:
899 487 264 51
SapI GCTCTTCn'nnn_
Cuts at: 0 1436 1463 1701
Size: 1436 27 238
Fragments arranged by size:
1436 238 27
SbfI CC_TGCA'GG
Cuts at: 0 1265 1409 1701
Size: 1265 144 292
Fragments arranged by size:
1265 292 144
SfcI C'TryA_G
Cuts at: 0 1148 1261 1405 1701
Size: 1148 113 144 296
Fragments arranged by size:
1148 296 144 113
SmlI C'TyrA_G
Cuts at: 0 1185 1701
Size: 1185 516
StuI AGG'CCT
Cuts at: 0 1300 1633 1701
Size: 1300 333 68
Fragments arranged by size:
1300 333 68
StyI C'CwwG_G
Cuts at: 0 32 1034 1521 1701
Size: 32 1002 487 180
Fragments arranged by size:
1002 487 180 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1534 1597 1701
Size: 1534 63 104
Fragments arranged by size:
1534 104 63
TatI w'GTAC_w
Cuts at: 0 129 1701
Size: 129 1572
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 576 608 1572 1604 1701
Size: 576 32 964 32 97
Fragments arranged by size:
964 576 97 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1051 1080 1701
Size: 1051 29 621
Fragments arranged by size:
1051 621 29
XmnI GAAnn'nnTTC
Cuts at: 0 709 1701
Size: 709 992
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AlwNI ApoI BanI BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI BsrGI BssSI
BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI
EarI EciI Eco57MI EcoRV FseI HaeII Hin4I HincII
KpnI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PciI PflMI PstI PvuII SapI SbfI SfcI
SmlI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI
BfrBI BglII BmtI BsaI BsaAI BsaBI BsaHI BsiWI
BspEI BspHI BspMI BsrDI BssHII BstAPI BstBI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoO109I EcoRI FalI FspI FspAI HindIII HpaI KasI
MfeI MluI MmeI NarI NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI