Restriction map for
pFN21AB9912
(Linear) (Six-base) MAPSORT of: KIBB9912.seq Check: 874 from: 1 to: 1776
KIBB9912.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 492 1046 1157 1776
Size: 464 28 554 111 619
Fragments arranged by size:
619 554 464 111 28
AhdI GACnn_n'nnGTC
Cuts at: 0 645 1776
Size: 645 1131
AlwNI CAG_nnn'CTG
Cuts at: 0 874 955 1776
Size: 874 81 821
Fragments arranged by size:
874 821 81
ApoI r'AATT_y
Cuts at: 0 609 1776
Size: 609 1167
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1753 1776
Size: 1753 23
BamHI G'GATC_C
Cuts at: 0 813 1050 1776
Size: 813 237 726
Fragments arranged by size:
813 726 237
BanI G'GyrC_C
Cuts at: 0 115 531 1776
Size: 115 416 1245
Fragments arranged by size:
1245 416 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1518 1552 1776
Size: 1518 34 224
Fragments arranged by size:
1518 224 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 713 1776
Size: 713 1063
BglII A'GATC_T
Cuts at: 0 1328 1776
Size: 1328 448
BmrI ACTGGGnnnn_n'
Cuts at: 0 53 650 1031 1776
Size: 53 597 381 745
Fragments arranged by size:
745 597 381 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1707 1734 1776
Size: 1707 27 42
Fragments arranged by size:
1707 42 27
Bpu10I CC'TnA_GC
Cuts at: 0 1195 1776
Size: 1195 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1776
Size: 982 794
BsaAI yAC'GTr
Cuts at: 0 377 910 1776
Size: 377 533 866
Fragments arranged by size:
866 533 377
BsaBI GATnn'nnATC
Cuts at: 0 875 956 1776
Size: 875 81 820
Fragments arranged by size:
875 820 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 201 231 275 305 1655 1685 1776
Size: 201 30 44 30 1350 30 91
Fragments arranged by size:
1350 201 91 44 30 30 30
BseYI C'CCAG_C
Cuts at: 0 1518 1776
Size: 1518 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1732 1776
Size: 1732 44
BsiWI C'GTAC_G
Cuts at: 0 398 1776
Size: 398 1378
BsmI GAATG_Cn'
Cuts at: 0 356 1776
Size: 356 1420
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1243 1704 1776
Size: 1243 461 72
Fragments arranged by size:
1243 461 72
BstYI r'GATC_y
Cuts at: 0 12 813 1050 1328 1718 1776
Size: 12 801 237 278 390 58
Fragments arranged by size:
801 390 278 237 58 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1717 1776
Size: 1717 59
BtsI GCAGTG_nn'
Cuts at: 0 153 1776
Size: 153 1623
ClaI AT'CG_AT
Cuts at: 0 879 1776
Size: 879 897
DraI TTT'AAA
Cuts at: 0 182 1776
Size: 182 1594
EaeI y'GGCC_r
Cuts at: 0 663 1776
Size: 663 1113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 492 1046 1157 1707 1734 1776
Size: 464 28 554 111 550 27 42
Fragments arranged by size:
554 550 464 111 42 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1713 1776
Size: 1713 63
EcoO109I rG'GnC_Cy
Cuts at: 0 1025 1191 1776
Size: 1025 166 585
Fragments arranged by size:
1025 585 166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1320 1776
Size: 1320 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 631 663 889 921 959 991 1776
Size: 631 32 226 32 38 32 785
Fragments arranged by size:
785 631 226 38 32 32 32
HincII GTy'rAC
Cuts at: 0 1436 1776
Size: 1436 340
HindIII A'AGCT_T
Cuts at: 0 504 1776
Size: 504 1272
MfeI C'AATT_G
Cuts at: 0 203 1776
Size: 203 1573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1335 1776
Size: 293 1042 441
Fragments arranged by size:
1042 441 293
MscI TGG'CCA
Cuts at: 0 665 1776
Size: 665 1111
MslI CAynn'nnrTG
Cuts at: 0 349 1160 1776
Size: 349 811 616
Fragments arranged by size:
811 616 349
NdeI CA'TA_TG
Cuts at: 0 324 414 1776
Size: 324 90 1362
Fragments arranged by size:
1362 324 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 1758 1776
Size: 1758 18
PfoI T'CCnGG_A
Cuts at: 0 1471 1776
Size: 1471 305
PmlI CAC'GTG
Cuts at: 0 910 1776
Size: 910 866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 955 987 1776
Size: 955 32 789
Fragments arranged by size:
955 789 32
PpuMI rG'GwC_Cy
Cuts at: 0 1025 1191 1776
Size: 1025 166 585
Fragments arranged by size:
1025 585 166
PsiI TTA'TAA
Cuts at: 0 689 1776
Size: 689 1087
PstI C_TGCA'G
Cuts at: 0 1236 1397 1776
Size: 1236 161 379
Fragments arranged by size:
1236 379 161
SfcI C'TryA_G
Cuts at: 0 362 1232 1311 1393 1776
Size: 362 870 79 82 383
Fragments arranged by size:
870 383 362 82 79
SmlI C'TyrA_G
Cuts at: 0 961 1776
Size: 961 815
SspI AAT'ATT
Cuts at: 0 1491 1575 1776
Size: 1491 84 201
Fragments arranged by size:
1491 201 84
StyI C'CwwG_G
Cuts at: 0 1071 1757 1776
Size: 1071 686 19
Fragments arranged by size:
1071 686 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 870 1071 1438 1776
Size: 870 201 367 338
Fragments arranged by size:
870 367 338 201
TatI w'GTAC_w
Cuts at: 0 827 1776
Size: 827 949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 894 926 1467 1499 1628 1660
Size: 275 32 587 32 541 32 129 32
Cuts at: 1660 1776
Size: 116
Fragments arranged by size:
587 541 275 129 116 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1150 1776
Size: 1150 626
XmnI GAAnn'nnTTC
Cuts at: 0 597 1776
Size: 597 1179
Enzymes that do cut:
AcuI AhdI AlwNI ApoI BaeI BamHI BanI BcgI
BciVI BglII BmrI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaXI BseYI BsgI BsiWI BsmI BstAPI BstYI BtgZI
BtsI ClaI DraI EaeI Eco57MI EcoNI EcoO109I FalI
Hin4I HincII HindIII MfeI MmeI MscI MslI NdeI
PflMI PfoI PmlI PpiI PpuMI PsiI PstI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BanII BbeI BbsI BbvCI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BsaI
BsaHI BsaWI BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraIII
DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XcmI XhoI XmaI ZraI