Restriction map for
pFN21AB9889
(Linear) (Six-base) MAPSORT of: KIBB9889.seq Check: 9603 from: 1 to: 3129
KIBB9889.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AccI GT'mk_AC
Cuts at: 0 2180 3129
Size: 2180 949
AclI AA'CG_TT
Cuts at: 0 2274 3129
Size: 2274 855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 661 1597 2097 2689 3129
Size: 146 515 936 500 592 440
Fragments arranged by size:
936 592 515 500 440 146
AleI CACnn'nnGTG
Cuts at: 0 1786 3129
Size: 1786 1343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1101 3129
Size: 1101 2028
ApaLI G'TGCA_C
Cuts at: 0 1159 3129
Size: 1159 1970
ApoI r'AATT_y
Cuts at: 0 50 70 85 268 345 1053 3124 3129
Size: 50 20 15 183 77 708 2071 5
Fragments arranged by size:
2071 708 183 77 50 20 15 5
AseI AT'TA_AT
Cuts at: 0 1166 2068 3129
Size: 1166 902 1061
Fragments arranged by size:
1166 1061 902
AvaI C'yCGr_G
Cuts at: 0 588 2161 3129
Size: 588 1573 968
Fragments arranged by size:
1573 968 588
AvrII C'CTAG_G
Cuts at: 0 411 3129
Size: 411 2718
BanI G'GyrC_C
Cuts at: 0 611 2119 3129
Size: 611 1508 1010
Fragments arranged by size:
1508 1010 611
BanII G_rGCy'C
Cuts at: 0 759 1218 1241 1387 2765 3129
Size: 759 459 23 146 1378 364
Fragments arranged by size:
1378 759 459 364 146 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 667 1580 1730 2226 3129
Size: 667 913 150 496 903
Fragments arranged by size:
913 903 667 496 150
BbvCI CC'TCA_GC
Cuts at: 0 1231 1435 3129
Size: 1231 204 1694
Fragments arranged by size:
1694 1231 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 311 345 983 1017 1498 1532 2678 2712
Size: 311 34 638 34 481 34 1146 34
Cuts at: 2712 3129
Size: 417
Fragments arranged by size:
1146 638 481 417 311 34 34 34
34
BfrBI ATG'CAT
Cuts at: 0 3031 3129
Size: 3031 98
BglII A'GATC_T
Cuts at: 0 881 2426 3129
Size: 881 1545 703
Fragments arranged by size:
1545 881 703
BlpI GC'TnA_GC
Cuts at: 0 694 2759 3129
Size: 694 2065 370
Fragments arranged by size:
2065 694 370
Bme1580I G_kGCm'C
Cuts at: 0 570 1163 2122 2716 3129
Size: 570 593 959 594 413
Fragments arranged by size:
959 594 593 570 413
BmgBI CAC'GTC
Cuts at: 0 1821 3129
Size: 1821 1308
BmrI ACTGGGnnnn_n'
Cuts at: 0 1648 3129
Size: 1648 1481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 773 2107 3129
Size: 773 1334 1022
Fragments arranged by size:
1334 1022 773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 196 558 1039 1381 2989 3129
Size: 170 26 362 481 342 1608 140
Fragments arranged by size:
1608 481 362 342 170 140 26
Bpu10I CC'TnA_GC
Cuts at: 0 758 1231 1435 3072 3129
Size: 758 473 204 1637 57
Fragments arranged by size:
1637 758 473 204 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1113 1225 2095 3129
Size: 646 467 112 870 1034
Fragments arranged by size:
1034 870 646 467 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 2349 3129
Size: 907 1442 780
Fragments arranged by size:
1442 907 780
BsaWI w'CCGG_w
Cuts at: 0 476 2093 2519 2921 3129
Size: 476 1617 426 402 208
Fragments arranged by size:
1617 476 426 402 208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1108 2641 3129
Size: 1108 1533 488
Fragments arranged by size:
1533 1108 488
BseYI C'CCAG_C
Cuts at: 0 2079 3129
Size: 2079 1050
BsiHKAI G_wGCw'C
Cuts at: 0 368 759 857 1163 1218 1241 1343 2765
Size: 368 391 98 306 55 23 102 1422
Cuts at: 2765 3129
Size: 364
Fragments arranged by size:
1422 391 368 364 306 102 98 55
23
BsmI GAATG_Cn'
Cuts at: 0 947 1527 2087 2937 3024 3129
Size: 947 580 560 850 87 105
Fragments arranged by size:
947 850 580 560 105 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1811 1999 3129
Size: 1811 188 1130
Fragments arranged by size:
1811 1130 188
Bsp1286I G_dGCh'C
Cuts at: 0 368 570 759 857 1163 1218 1241 1343
Size: 368 202 189 98 306 55 23 102
Cuts at: 1343 1387 2122 2716 2765 3129
Size: 44 735 594 49 364
Fragments arranged by size:
735 594 368 364 306 202 189 102
98 55 49 44 23
BspEI T'CCGG_A
Cuts at: 0 476 2093 2519 2921 3129
Size: 476 1617 426 402 208
Fragments arranged by size:
1617 476 426 402 208
BspHI T'CATG_A
Cuts at: 0 785 1886 2546 2579 3129
Size: 785 1101 660 33 550
Fragments arranged by size:
1101 785 660 550 33
BsrDI GCAATG_nn'
Cuts at: 0 461 1095 1664 2033 2412 3129
Size: 461 634 569 369 379 717
Fragments arranged by size:
717 634 569 461 379 369
BsrFI r'CCGG_y
Cuts at: 0 1178 1226 2122 3129
Size: 1178 48 896 1007
Fragments arranged by size:
1178 1007 896 48
BsrGI T'GTAC_A
Cuts at: 0 1460 2987 3129
Size: 1460 1527 142
Fragments arranged by size:
1527 1460 142
BssSI C'ACGA_G
Cuts at: 0 2002 2635 3129
Size: 2002 633 494
Fragments arranged by size:
2002 633 494
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2211 3129
Size: 2211 918
BstYI r'GATC_y
Cuts at: 0 106 881 1744 2426 2658 3129
Size: 106 775 863 682 232 471
Fragments arranged by size:
863 775 682 471 232 106
Bsu36I CC'TnA_GG
Cuts at: 0 3062 3129
Size: 3062 67
BtgI C'CryG_G
Cuts at: 0 387 602 3129
Size: 387 215 2527
Fragments arranged by size:
2527 387 215
BtsI GCAGTG_nn'
Cuts at: 0 570 644 845 2067 3129
Size: 570 74 201 1222 1062
Fragments arranged by size:
1222 1062 570 201 74
ClaI AT'CG_AT
Cuts at: 0 482 3129
Size: 482 2647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5 40 3129
Size: 5 35 3089
Fragments arranged by size:
3089 35 5
DraIII CAC_nnn'GTG
Cuts at: 0 861 3129
Size: 861 2268
DrdI GACnn_nn'nnGTC
Cuts at: 0 737 3129
Size: 737 2392
EaeI y'GGCC_r
Cuts at: 0 451 1412 3129
Size: 451 961 1717
Fragments arranged by size:
1717 961 451
EarI CTCTTCn'nnn_
Cuts at: 0 664 747 2050 3129
Size: 664 83 1303 1079
Fragments arranged by size:
1303 1079 664 83
EcoICRI GAG'CTC
Cuts at: 0 757 1216 1239 2763 3129
Size: 757 459 23 1524 366
Fragments arranged by size:
1524 757 459 366 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 170 196 558 661 1039 1381 1597
Size: 146 24 26 362 103 378 342 216
Cuts at: 1597 2097 2689 2989 3129
Size: 500 592 300 140
Fragments arranged by size:
592 500 378 362 342 300 216 146
140 103 26 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 3129
Size: 199 2930
EcoO109I rG'GnC_Cy
Cuts at: 0 3078 3129
Size: 3078 51
EcoRI G'AATT_C
Cuts at: 0 345 1053 3129
Size: 345 708 2076
Fragments arranged by size:
2076 708 345
EcoRV GAT'ATC
Cuts at: 0 379 997 3129
Size: 379 618 2132
Fragments arranged by size:
2132 618 379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 171 334 948 2127 3129
Size: 171 163 614 1179 1002
Fragments arranged by size:
1179 1002 614 171 163
FseI GG_CCGG'CC
Cuts at: 0 1182 3129
Size: 1182 1947
HaeII r_GCGC'y
Cuts at: 0 2919 3129
Size: 2919 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 368 400 487 519 657 689 1276 1308
Size: 368 32 87 32 138 32 587 32
Cuts at: 1308 1606 1638 3129
Size: 298 32 1491
Fragments arranged by size:
1491 587 368 298 138 87 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 501 1425 3129
Size: 501 924 1704
Fragments arranged by size:
1704 924 501
HindIII A'AGCT_T
Cuts at: 0 3069 3129
Size: 3069 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2318 3129
Size: 2318 811
MscI TGG'CCA
Cuts at: 0 1414 3129
Size: 1414 1715
MslI CAynn'nnrTG
Cuts at: 0 1786 1838 1885 2551 3129
Size: 1786 52 47 666 578
Fragments arranged by size:
1786 666 578 52 47
MspA1I CmG'CkG
Cuts at: 0 1402 1439 2413 3129
Size: 1402 37 974 716
Fragments arranged by size:
1402 974 716 37
NaeI GCC'GGC
Cuts at: 0 1180 3129
Size: 1180 1949
NcoI C'CATG_G
Cuts at: 0 387 602 3129
Size: 387 215 2527
Fragments arranged by size:
2527 387 215
NgoMIV G'CCGG_C
Cuts at: 0 1178 3129
Size: 1178 1951
NsiI A_TGCA'T
Cuts at: 0 3033 3129
Size: 3033 96
NspI r_CATG'y
Cuts at: 0 931 1513 3129
Size: 931 582 1616
Fragments arranged by size:
1616 931 582
PflMI CCAn_nnn'nTGG
Cuts at: 0 388 608 3129
Size: 388 220 2521
Fragments arranged by size:
2521 388 220
PfoI T'CCnGG_A
Cuts at: 0 194 3129
Size: 194 2935
PpuMI rG'GwC_Cy
Cuts at: 0 3078 3129
Size: 3078 51
PspXI vC'TCGA_Gb
Cuts at: 0 2161 3129
Size: 2161 968
PstI C_TGCA'G
Cuts at: 0 808 1397 1444 3129
Size: 808 589 47 1685
Fragments arranged by size:
1685 808 589 47
PvuII CAG'CTG
Cuts at: 0 1402 1439 2413 3129
Size: 1402 37 974 716
Fragments arranged by size:
1402 974 716 37
SacI G_AGCT'C
Cuts at: 0 759 1218 1241 2765 3129
Size: 759 459 23 1524 364
Fragments arranged by size:
1524 759 459 364 23
SanDI GG'GwC_CC
Cuts at: 0 3078 3129
Size: 3078 51
SapI GCTCTTCn'nnn_
Cuts at: 0 747 2050 3129
Size: 747 1303 1079
Fragments arranged by size:
1303 1079 747
SfcI C'TryA_G
Cuts at: 0 804 1393 1440 1847 3129
Size: 804 589 47 407 1282
Fragments arranged by size:
1282 804 589 407 47
SmlI C'TyrA_G
Cuts at: 0 661 1128 1240 2074 2161 3129
Size: 661 467 112 834 87 968
Fragments arranged by size:
968 834 661 467 112 87
SphI G_CATG'C
Cuts at: 0 1513 3129
Size: 1513 1616
StuI AGG'CCT
Cuts at: 0 416 2159 3129
Size: 416 1743 970
Fragments arranged by size:
1743 970 416
StyI C'CwwG_G
Cuts at: 0 23 387 411 467 602 3129
Size: 23 364 24 56 135 2527
Fragments arranged by size:
2527 364 135 56 24 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 74 3129
Size: 33 41 3055
Fragments arranged by size:
3055 41 33
TatI w'GTAC_w
Cuts at: 0 1460 2188 2732 2987 3129
Size: 1460 728 544 255 142
Fragments arranged by size:
1460 728 544 255 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 610 642 3129
Size: 610 32 2487
Fragments arranged by size:
2487 610 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2398 2592 3129
Size: 2398 194 537
Fragments arranged by size:
2398 537 194
XhoI C'TCGA_G
Cuts at: 0 2161 3129
Size: 2161 968
XmnI GAAnn'nnTTC
Cuts at: 0 893 3129
Size: 893 2236
Enzymes that do cut:
AccI AclI AcuI AleI AlfI ApaLI ApoI AseI
AvaI AvrII BanI BanII BbsI BbvCI BcgI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BssSI BstXI
BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI HaeII Hin4I HincII HindIII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI
PfoI PpuMI PspXI PstI PvuII SacI SanDI SapI
SfcI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI AscI AsiSI BaeI BamHI BbeI
BciVI BclI BglI BmtI BsaAI BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BspMI BsrBI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI DraI EagI EciI FspI FspAI
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI
ZraI