Restriction map for
pFN21AB9862
(Linear) (Six-base) MAPSORT of: KIBB9862.seq Check: 6774 from: 1 to: 1248
KIBB9862.orf
With 182 enzymes: SgfI *
July 3, 2012 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 168 650 1069 1248
Size: 168 482 419 179
Fragments arranged by size:
482 419 179 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 320 1248
Size: 233 87 928
Fragments arranged by size:
928 233 87
AflIII A'CryG_T
Cuts at: 0 569 1248
Size: 569 679
AlwNI CAG_nnn'CTG
Cuts at: 0 1020 1248
Size: 1020 228
ApaI G_GGCC'C
Cuts at: 0 717 1248
Size: 717 531
AvaI C'yCGr_G
Cuts at: 0 209 237 630 738 763 1248
Size: 209 28 393 108 25 485
Fragments arranged by size:
485 393 209 108 28 25
BanI G'GyrC_C
Cuts at: 0 259 273 840 879 1222 1248
Size: 259 14 567 39 343 26
Fragments arranged by size:
567 343 259 39 26 14
BanII G_rGCy'C
Cuts at: 0 639 717 1248
Size: 639 78 531
Fragments arranged by size:
639 531 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 113 125 1248
Size: 74 39 12 1123
Fragments arranged by size:
1123 74 39 12
BbvCI CC'TCA_GC
Cuts at: 0 913 1248
Size: 913 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 784 818 1248
Size: 784 34 430
Fragments arranged by size:
784 430 34
BglI GCCn_nnn'nGGC
Cuts at: 0 527 1239 1248
Size: 527 712 9
Fragments arranged by size:
712 527 9
BlpI GC'TnA_GC
Cuts at: 0 17 391 1248
Size: 17 374 857
Fragments arranged by size:
857 374 17
Bme1580I G_kGCm'C
Cuts at: 0 276 717 843 1225 1248
Size: 276 441 126 382 23
Fragments arranged by size:
441 382 276 126 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 564 1050 1248
Size: 148 416 486 198
Fragments arranged by size:
486 416 198 148
Bpu10I CC'TnA_GC
Cuts at: 0 913 1248
Size: 913 335
BsaHI Gr'CG_yC
Cuts at: 0 771 942 1248
Size: 771 171 306
Fragments arranged by size:
771 306 171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 1248
Size: 544 30 674
Fragments arranged by size:
674 544 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 139 142 145 939 1248
Size: 109 30 3 3 794 309
Fragments arranged by size:
794 309 109 30 3 3
BseYI C'CCAG_C
Cuts at: 0 1214 1248
Size: 1214 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 667 772 1248
Size: 653 14 105 476
Fragments arranged by size:
653 476 105 14
BsiEI CG_ry'CG
Cuts at: 0 1042 1248
Size: 1042 206
BsiHKAI G_wGCw'C
Cuts at: 0 218 1248
Size: 218 1030
BsmBI CGTCTCn'nnnn_
Cuts at: 0 762 1248
Size: 762 486
Bsp1286I G_dGCh'C
Cuts at: 0 218 276 639 717 843 1225 1248
Size: 218 58 363 78 126 382 23
Fragments arranged by size:
382 363 218 126 78 58 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 168 650 672 1069 1248
Size: 14 154 482 22 397 179
Fragments arranged by size:
482 397 179 154 22 14
BsrBI CCG'CTC
Cuts at: 0 1008 1248
Size: 1008 240
BsrFI r'CCGG_y
Cuts at: 0 315 1248
Size: 315 933
BstYI r'GATC_y
Cuts at: 0 503 1248
Size: 503 745
BtgI C'CryG_G
Cuts at: 0 353 1248
Size: 353 895
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 324 1248
Size: 324 924
ClaI AT'CG_AT
Cuts at: 0 80 1248
Size: 80 1168
EaeI y'GGCC_r
Cuts at: 0 356 417 1248
Size: 356 61 831
Fragments arranged by size:
831 356 61
EarI CTCTTCn'nnn_
Cuts at: 0 806 1248
Size: 806 442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 457 973 1112 1248
Size: 457 516 139 136
Fragments arranged by size:
516 457 139 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 233 320 564 1050 1248
Size: 148 85 87 244 486 198
Fragments arranged by size:
486 244 198 148 87 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 1248
Size: 91 1157
EcoO109I rG'GnC_Cy
Cuts at: 0 700 713 714 743 1248
Size: 700 13 1 29 505
Fragments arranged by size:
700 505 29 13 1
FspI TGC'GCA
Cuts at: 0 1024 1248
Size: 1024 224
HaeII r_GCGC'y
Cuts at: 0 24 384 618 1245 1248
Size: 24 360 234 627 3
Fragments arranged by size:
627 360 234 24 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 544 576 1248
Size: 203 32 309 32 672
Fragments arranged by size:
672 309 203 32 32
MscI TGG'CCA
Cuts at: 0 358 1248
Size: 358 890
MslI CAynn'nnrTG
Cuts at: 0 352 1248
Size: 352 896
MspA1I CmG'CkG
Cuts at: 0 395 1101 1131 1248
Size: 395 706 30 117
Fragments arranged by size:
706 395 117 30
NaeI GCC'GGC
Cuts at: 0 317 1248
Size: 317 931
NcoI C'CATG_G
Cuts at: 0 353 1248
Size: 353 895
NgoMIV G'CCGG_C
Cuts at: 0 315 1248
Size: 315 933
NspI r_CATG'y
Cuts at: 0 573 1248
Size: 573 675
PciI A'CATG_T
Cuts at: 0 569 1248
Size: 569 679
PfoI T'CCnGG_A
Cuts at: 0 50 1248
Size: 50 1198
PspOMI G'GGCC_C
Cuts at: 0 713 1248
Size: 713 535
PstI C_TGCA'G
Cuts at: 0 179 898 1248
Size: 179 719 350
Fragments arranged by size:
719 350 179
SfcI C'TryA_G
Cuts at: 0 175 894 1248
Size: 175 719 354
Fragments arranged by size:
719 354 175
SmaI CCC'GGG
Cuts at: 0 632 740 765 1248
Size: 632 108 25 483
Fragments arranged by size:
632 483 108 25
StyI C'CwwG_G
Cuts at: 0 280 353 918 1201 1248
Size: 280 73 565 283 47
Fragments arranged by size:
565 283 280 73 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 539 710 1248
Size: 539 171 538
Fragments arranged by size:
539 538 171
XmaI C'CCGG_G
Cuts at: 0 630 738 763 1248
Size: 630 108 25 485
Fragments arranged by size:
630 485 108 25
XmnI GAAnn'nnTTC
Cuts at: 0 607 1248
Size: 607 641
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BcgI BglI BlpI Bme1580I BpmI Bpu10I
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BstYI BtgI BtgZI ClaI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PfoI PspOMI PstI SfcI SmaI StyI TaqII
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI ZraI