Restriction map for
pFN21AB9813
(Linear) (Six-base) MAPSORT of: KIBB9813.seq Check: 5363 from: 1 to: 816
KIBB9813.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 816
Size: 53 763
AleI CACnn'nnGTG
Cuts at: 0 181 816
Size: 181 635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 816
Size: 199 617
AlwNI CAG_nnn'CTG
Cuts at: 0 774 816
Size: 774 42
ApaI G_GGCC'C
Cuts at: 0 205 572 816
Size: 205 367 244
Fragments arranged by size:
367 244 205
AvaI C'yCGr_G
Cuts at: 0 339 430 604 733 816
Size: 339 91 174 129 83
Fragments arranged by size:
339 174 129 91 83
BanI G'GyrC_C
Cuts at: 0 78 85 175 326 799 816
Size: 78 7 90 151 473 17
Fragments arranged by size:
473 151 90 78 17 7
BanII G_rGCy'C
Cuts at: 0 154 205 572 816
Size: 154 51 367 244
Fragments arranged by size:
367 244 154 51
BbeI G_GCGC'C
Cuts at: 0 82 330 803 816
Size: 82 248 473 13
Fragments arranged by size:
473 248 82 13
BbvCI CC'TCA_GC
Cuts at: 0 553 816
Size: 553 263
BglI GCCn_nnn'nGGC
Cuts at: 0 582 737 797 816
Size: 582 155 60 19
Fragments arranged by size:
582 155 60 19
BlpI GC'TnA_GC
Cuts at: 0 136 816
Size: 136 680
Bme1580I G_kGCm'C
Cuts at: 0 205 572 816
Size: 205 367 244
Fragments arranged by size:
367 244 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 409 472 816
Size: 184 225 63 344
Fragments arranged by size:
344 225 184 63
Bpu10I CC'TnA_GC
Cuts at: 0 553 816
Size: 553 263
BsaHI Gr'CG_yC
Cuts at: 0 79 327 800 816
Size: 79 248 473 16
Fragments arranged by size:
473 248 79 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 88 367 816
Size: 85 3 279 449
Fragments arranged by size:
449 279 85 3
BseYI C'CCAG_C
Cuts at: 0 675 707 809 816
Size: 675 32 102 7
Fragments arranged by size:
675 102 32 7
BsiEI CG_ry'CG
Cuts at: 0 65 245 269 282 816
Size: 65 180 24 13 534
Fragments arranged by size:
534 180 65 24 13
BsiWI C'GTAC_G
Cuts at: 0 720 816
Size: 720 96
Bsp1286I G_dGCh'C
Cuts at: 0 154 205 572 816
Size: 154 51 367 244
Fragments arranged by size:
367 244 154 51
BsrFI r'CCGG_y
Cuts at: 0 323 393 625 816
Size: 323 70 232 191
Fragments arranged by size:
323 232 191 70
BssHII G'CGCG_C
Cuts at: 0 121 123 420 422 615 816
Size: 121 2 297 2 193 201
Fragments arranged by size:
297 201 193 121 2 2
BtgI C'CryG_G
Cuts at: 0 24 635 816
Size: 24 611 181
Fragments arranged by size:
611 181 24
EaeI y'GGCC_r
Cuts at: 0 62 242 266 473 506 665 816
Size: 62 180 24 207 33 159 151
Fragments arranged by size:
207 180 159 151 62 33 24
EagI C'GGCC_G
Cuts at: 0 62 242 266 816
Size: 62 180 24 550
Fragments arranged by size:
550 180 62 24
EarI CTCTTCn'nnn_
Cuts at: 0 605 816
Size: 605 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 289 727 816
Size: 289 438 89
Fragments arranged by size:
438 289 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 184 409 472 816
Size: 53 131 225 63 344
Fragments arranged by size:
344 225 131 63 53
EcoO109I rG'GnC_Cy
Cuts at: 0 261 568 816
Size: 261 307 248
Fragments arranged by size:
307 261 248
HaeII r_GCGC'y
Cuts at: 0 82 330 370 376 388 445 643 750
Size: 82 248 40 6 12 57 198 107
Cuts at: 750 803 816
Size: 53 13
Fragments arranged by size:
248 198 107 82 57 53 40 13
12 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 816
Size: 448 32 336
Fragments arranged by size:
448 336 32
KasI G'GCGC_C
Cuts at: 0 78 326 799 816
Size: 78 248 473 17
Fragments arranged by size:
473 248 78 17
MslI CAynn'nnrTG
Cuts at: 0 181 649 816
Size: 181 468 167
Fragments arranged by size:
468 181 167
MspA1I CmG'CkG
Cuts at: 0 26 54 683 816
Size: 26 28 629 133
Fragments arranged by size:
629 133 28 26
NaeI GCC'GGC
Cuts at: 0 325 395 627 816
Size: 325 70 232 189
Fragments arranged by size:
325 232 189 70
NarI GG'CG_CC
Cuts at: 0 79 327 800 816
Size: 79 248 473 16
Fragments arranged by size:
473 248 79 16
NgoMIV G'CCGG_C
Cuts at: 0 323 393 625 816
Size: 323 70 232 191
Fragments arranged by size:
323 232 191 70
PfoI T'CCnGG_A
Cuts at: 0 587 816
Size: 587 229
PspOMI G'GGCC_C
Cuts at: 0 201 568 816
Size: 201 367 248
Fragments arranged by size:
367 248 201
PspXI vC'TCGA_Gb
Cuts at: 0 430 604 733 816
Size: 430 174 129 83
Fragments arranged by size:
430 174 129 83
PstI C_TGCA'G
Cuts at: 0 380 767 816
Size: 380 387 49
Fragments arranged by size:
387 380 49
PvuII CAG'CTG
Cuts at: 0 54 816
Size: 54 762
SacII CC_GC'GG
Cuts at: 0 27 816
Size: 27 789
SapI GCTCTTCn'nnn_
Cuts at: 0 605 816
Size: 605 211
SexAI A'CCwGG_T
Cuts at: 0 533 816
Size: 533 283
SfcI C'TryA_G
Cuts at: 0 376 763 816
Size: 376 387 53
Fragments arranged by size:
387 376 53
SfoI GGC'GCC
Cuts at: 0 80 328 801 816
Size: 80 248 473 15
Fragments arranged by size:
473 248 80 15
SgrAI Cr'CCGG_yG
Cuts at: 0 323 816
Size: 323 493
SmaI CCC'GGG
Cuts at: 0 341 816
Size: 341 475
SmlI C'TyrA_G
Cuts at: 0 430 604 733 816
Size: 430 174 129 83
Fragments arranged by size:
430 174 129 83
StyI C'CwwG_G
Cuts at: 0 146 179 816
Size: 146 33 637
Fragments arranged by size:
637 146 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 816
Size: 585 231
XhoI C'TCGA_G
Cuts at: 0 430 604 733 816
Size: 430 174 129 83
Fragments arranged by size:
430 174 129 83
XmaI C'CCGG_G
Cuts at: 0 339 816
Size: 339 477
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbvCI BglI BlpI Bme1580I BpmI Bpu10I BsaHI
BseRI BseYI BsiEI BsiWI Bsp1286I BsrFI BssHII BtgI
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
KasI MslI MspA1I NaeI NarI NgoMIV PfoI PspOMI
PspXI PstI PvuII SacII SapI SexAI SfcI SfoI
SgrAI SmaI SmlI StyI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiHKAI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII
SacI SalI SanDI SbfI ScaI SfiI SgfI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XmnI ZraI