Restriction map for
pFN21AB9768
(Linear) (Six-base) MAPSORT of: KIBB9768.seq Check: 3989 from: 1 to: 3018
KIBB9768.orf
With 182 enzymes: SgfI *
July 29, 2015 17:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 785 3018
Size: 785 2233
AatII G_ACGT'C
Cuts at: 0 719 3018
Size: 719 2299
Acc65I G'GTAC_C
Cuts at: 0 746 3018
Size: 746 2272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1740 2058 3018
Size: 140 1600 318 960
Fragments arranged by size:
1600 960 318 140
AflIII A'CryG_T
Cuts at: 0 1586 3018
Size: 1586 1432
AgeI A'CCGG_T
Cuts at: 0 2918 3018
Size: 2918 100
AleI CACnn'nnGTG
Cuts at: 0 516 700 1055 3018
Size: 516 184 355 1963
Fragments arranged by size:
1963 516 355 184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 534 3018
Size: 534 2484
AlwNI CAG_nnn'CTG
Cuts at: 0 254 465 660 3018
Size: 254 211 195 2358
Fragments arranged by size:
2358 254 211 195
ApaI G_GGCC'C
Cuts at: 0 315 349 1432 1448 1569 1598 1719 1921
Size: 315 34 1083 16 121 29 121 202
Cuts at: 1921 3018
Size: 1097
Fragments arranged by size:
1097 1083 315 202 121 121 34 29
16
ApaLI G'TGCA_C
Cuts at: 0 1021 1648 1780 2098 2454 3018
Size: 1021 627 132 318 356 564
Fragments arranged by size:
1021 627 564 356 318 132
AscI GG'CGCG_CC
Cuts at: 0 2645 3018
Size: 2645 373
AvaI C'yCGr_G
Cuts at: 0 126 1710 1816 1914 1960 2004 2160 2296
Size: 126 1584 106 98 46 44 156 136
Cuts at: 2296 2485 2661 2734 2750 2818 3018
Size: 189 176 73 16 68 200
Fragments arranged by size:
1584 200 189 176 156 136 126 106
98 73 68 46 44 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2913 2946 3018
Size: 2913 33 72
Fragments arranged by size:
2913 72 33
BanI G'GyrC_C
Cuts at: 0 85 114 537 746 805 1010 1260 1539
Size: 85 29 423 209 59 205 250 279
Cuts at: 1539 1605 1681 1848 2257 2405 2427 2841 2895
Size: 66 76 167 409 148 22 414 54
Cuts at: 2895 3018
Size: 123
Fragments arranged by size:
423 414 409 279 250 209 205 167
148 123 85 76 66 59 54 29
22
BanII G_rGCy'C
Cuts at: 0 133 315 349 406 581 626 1364 1432
Size: 133 182 34 57 175 45 738 68
Cuts at: 1432 1448 1531 1569 1598 1706 1719 1921 2005
Size: 16 83 38 29 108 13 202 84
Cuts at: 2005 2424 2774 3018
Size: 419 350 244
Fragments arranged by size:
738 419 350 244 202 182 175 133
108 84 83 68 57 45 38 34
29 16 13
BbeI G_GCGC'C
Cuts at: 0 1264 1609 1685 1852 2261 2409 2845 2899
Size: 1264 345 76 167 409 148 436 54
Cuts at: 2899 3018
Size: 119
Fragments arranged by size:
1264 436 409 345 167 148 119 76
54
BbsI GAAGACnn'nnnn_
Cuts at: 0 711 3018
Size: 711 2307
BbvCI CC'TCA_GC
Cuts at: 0 445 3018
Size: 445 2573
BclI T'GATC_A
Cuts at: 0 704 3018
Size: 704 2314
BglI GCCn_nnn'nGGC
Cuts at: 0 679 803 1127 2497 3018
Size: 679 124 324 1370 521
Fragments arranged by size:
1370 679 521 324 124
BglII A'GATC_T
Cuts at: 0 1142 3018
Size: 1142 1876
BlpI GC'TnA_GC
Cuts at: 0 1036 3018
Size: 1036 1982
Bme1580I G_kGCm'C
Cuts at: 0 315 349 634 808 1025 1432 1448 1544
Size: 315 34 285 174 217 407 16 96
Cuts at: 1544 1569 1598 1652 1719 1784 1921 2102 2458
Size: 25 29 54 67 65 137 181 356
Cuts at: 2458 2521 2890 3018
Size: 63 369 128
Fragments arranged by size:
407 369 356 315 285 217 181 174
137 128 96 67 65 63 54 34
29 25 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 879 3018
Size: 879 2139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1164 3018
Size: 1164 1854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 295 637 940 1515 2629 3018
Size: 276 19 342 303 575 1114 389
Fragments arranged by size:
1114 575 389 342 303 276 19
Bpu10I CC'TnA_GC
Cuts at: 0 445 556 2389 3018
Size: 445 111 1833 629
Fragments arranged by size:
1833 629 445 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 3018
Size: 1000 2018
BsaI GGTCTCn'nnnn_
Cuts at: 0 844 2080 3018
Size: 844 1236 938
Fragments arranged by size:
1236 938 844
BsaAI yAC'GTr
Cuts at: 0 629 1587 1733 1779 3012 3018
Size: 629 958 146 46 1233 6
Fragments arranged by size:
1233 958 629 146 46 6
BsaHI Gr'CG_yC
Cuts at: 0 716 1261 1465 1606 1682 1849 2226 2258
Size: 716 545 204 141 76 167 377 32
Cuts at: 2258 2406 2680 2842 2867 2896 3018
Size: 148 274 162 25 29 122
Fragments arranged by size:
716 545 377 274 204 167 162 148
141 122 76 32 29 25
BsaWI w'CCGG_w
Cuts at: 0 2918 3018
Size: 2918 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 635 665 3018
Size: 61 30 544 30 2353
Fragments arranged by size:
2353 544 61 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 556 1417 1948 2982 3018
Size: 82 474 861 531 1034 36
Fragments arranged by size:
1034 861 531 474 82 36
BseYI C'CCAG_C
Cuts at: 0 136 405 665 904 1167 1551 1942 2038
Size: 136 269 260 239 263 384 391 96
Cuts at: 2038 2878 3018
Size: 840 140
Fragments arranged by size:
840 391 384 269 263 260 239 140
136 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 307 540 626 2230 3018
Size: 94 213 233 86 1604 788
Fragments arranged by size:
1604 788 233 213 94 86
BsiEI CG_ry'CG
Cuts at: 0 575 821 1980 2149 2153 2180 2255 2372
Size: 575 246 1159 169 4 27 75 117
Cuts at: 2372 2687 2691 2810 3018
Size: 315 4 119 208
Fragments arranged by size:
1159 575 315 246 208 169 119 117
75 27 4 4
BsiHKAI G_wGCw'C
Cuts at: 0 566 1025 1652 1784 2102 2458 3011 3018
Size: 566 459 627 132 318 356 553 7
Fragments arranged by size:
627 566 553 459 356 318 132 7
Bsp1286I G_dGCh'C
Cuts at: 0 133 315 349 406 566 581 626 634
Size: 133 182 34 57 160 15 45 8
Cuts at: 634 808 1025 1364 1432 1448 1531 1544 1569
Size: 174 217 339 68 16 83 13 25
Cuts at: 1569 1598 1652 1706 1719 1784 1921 2005 2102
Size: 29 54 54 13 65 137 84 97
Cuts at: 2102 2424 2458 2521 2774 2890 3011 3018
Size: 322 34 63 253 116 121 7
Fragments arranged by size:
339 322 253 217 182 174 160 137
133 121 116 97 84 83 68 65
63 57 54 54 45 34 34 29
25 16 15 13 13 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 773 785 2612 3018
Size: 773 12 1827 406
Fragments arranged by size:
1827 773 406 12
BsrBI CCG'CTC
Cuts at: 0 260 951 1008 1040 1327 2412 2707 3018
Size: 260 691 57 32 287 1085 295 311
Fragments arranged by size:
1085 691 311 295 287 260 57 32
BsrFI r'CCGG_y
Cuts at: 0 279 534 808 1655 1684 2148 2175 2254
Size: 279 255 274 847 29 464 27 79
Cuts at: 2254 2430 2656 2682 2838 2892 2918 3018
Size: 176 226 26 156 54 26 100
Fragments arranged by size:
847 464 279 274 255 226 176 156
100 79 54 29 27 26 26
BssHII G'CGCG_C
Cuts at: 0 1691 1834 1868 1927 2350 2540 2645 3018
Size: 1691 143 34 59 423 190 105 373
Fragments arranged by size:
1691 423 373 190 143 105 59 34
BssSI C'ACGA_G
Cuts at: 0 2083 2449 2514 3018
Size: 2083 366 65 504
Fragments arranged by size:
2083 504 366 65
BstAPI GCAn_nnn'nTGC
Cuts at: 0 899 2051 2462 2634 3018
Size: 899 1152 411 172 384
Fragments arranged by size:
1152 899 411 384 172
BstEII G'GTnAC_C
Cuts at: 0 525 1101 3018
Size: 525 576 1917
Fragments arranged by size:
1917 576 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 201 918 1057 1113 3018
Size: 96 105 717 139 56 1905
Fragments arranged by size:
1905 717 139 105 96 56
BstYI r'GATC_y
Cuts at: 0 491 923 1142 1622 3018
Size: 491 432 219 480 1396
Fragments arranged by size:
1396 491 480 432 219
BtgI C'CryG_G
Cuts at: 0 110 230 329 354 1244 2015 2305 2366
Size: 110 120 99 25 890 771 290 61
Cuts at: 2366 2401 2691 2742 3018
Size: 35 290 51 276
Fragments arranged by size:
890 771 290 290 276 120 110 99
61 51 35 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1256 2952 3018
Size: 1256 1696 66
Fragments arranged by size:
1696 1256 66
BtsI GCAGTG_nn'
Cuts at: 0 707 3018
Size: 707 2311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 717 752 3018
Size: 717 35 2266
Fragments arranged by size:
2266 717 35
DraIII CAC_nnn'GTG
Cuts at: 0 603 2051 3018
Size: 603 1448 967
Fragments arranged by size:
1448 967 603
DrdI GACnn_nn'nnGTC
Cuts at: 0 2866 3018
Size: 2866 152
EaeI y'GGCC_r
Cuts at: 0 572 594 818 1298 1490 1559 1628 1878
Size: 572 22 224 480 192 69 69 250
Cuts at: 1878 1952 1977 2150 2177 2252 2280 2369 2438
Size: 74 25 173 27 75 28 89 69
Cuts at: 2438 2654 2684 2807 3018
Size: 216 30 123 211
Fragments arranged by size:
572 480 250 224 216 211 192 173
123 89 75 74 69 69 69 30
28 27 25 22
EagI C'GGCC_G
Cuts at: 0 572 818 1977 2150 2177 2252 2369 2684
Size: 572 246 1159 173 27 75 117 315
Cuts at: 2684 2807 3018
Size: 123 211
Fragments arranged by size:
1159 572 315 246 211 173 123 117
75 27
EarI CTCTTCn'nnn_
Cuts at: 0 233 1711 3018
Size: 233 1478 1307
Fragments arranged by size:
1478 1307 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 409 2608 3018
Size: 24 385 2199 410
Fragments arranged by size:
2199 410 385 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 276 295 637 940 1515 1740 2058
Size: 140 136 19 342 303 575 225 318
Cuts at: 2058 2629 3018
Size: 571 389
Fragments arranged by size:
575 571 389 342 318 303 225 140
136 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 469 1300 3018
Size: 469 831 1718
Fragments arranged by size:
1718 831 469
EcoO109I rG'GnC_Cy
Cuts at: 0 190 345 462 1231 1293 1429 1445 1452
Size: 190 155 117 769 62 136 16 7
Cuts at: 1452 1565 1566 1715 1716 1918 2164 2601 2738
Size: 113 1 149 1 202 246 437 137
Cuts at: 2738 3018
Size: 280
Fragments arranged by size:
769 437 280 246 202 190 155 149
137 136 117 113 62 16 7 1
1
FseI GG_CCGG'CC
Cuts at: 0 2179 2660 3018
Size: 2179 481 358
Fragments arranged by size:
2179 481 358
FspI TGC'GCA
Cuts at: 0 934 2215 3018
Size: 934 1281 803
Fragments arranged by size:
1281 934 803
FspAI rTGC'GCAy
Cuts at: 0 2215 3018
Size: 2215 803
HaeII r_GCGC'y
Cuts at: 0 911 1264 1609 1685 1730 1791 1852 2261
Size: 911 353 345 76 45 61 61 409
Cuts at: 2261 2409 2437 2845 2899 3018
Size: 148 28 408 54 119
Fragments arranged by size:
911 409 408 353 345 148 119 76
61 61 54 45 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 308 340 3018
Size: 136 32 140 32 2678
Fragments arranged by size:
2678 140 136 32 32
HincII GTy'rAC
Cuts at: 0 150 1767 3018
Size: 150 1617 1251
Fragments arranged by size:
1617 1251 150
HpaI GTT'AAC
Cuts at: 0 150 3018
Size: 150 2868
KasI G'GCGC_C
Cuts at: 0 1260 1605 1681 1848 2257 2405 2841 2895
Size: 1260 345 76 167 409 148 436 54
Cuts at: 2895 3018
Size: 123
Fragments arranged by size:
1260 436 409 345 167 148 123 76
54
KpnI G_GTAC'C
Cuts at: 0 750 3018
Size: 750 2268
MscI TGG'CCA
Cuts at: 0 1630 3018
Size: 1630 1388
MslI CAynn'nnrTG
Cuts at: 0 359 516 700 1055 1387 3018
Size: 359 157 184 355 332 1631
Fragments arranged by size:
1631 359 355 332 184 157
MspA1I CmG'CkG
Cuts at: 0 165 944 1055 1246 2307 2368 2403 2524
Size: 165 779 111 191 1061 61 35 121
Cuts at: 2524 2693 2901 3018
Size: 169 208 117
Fragments arranged by size:
1061 779 208 191 169 165 121 117
111 61 35
NaeI GCC'GGC
Cuts at: 0 1657 1686 2177 2256 2658 2684 2840 2894
Size: 1657 29 491 79 402 26 156 54
Cuts at: 2894 3018
Size: 124
Fragments arranged by size:
1657 491 402 156 124 79 54 29
26
NarI GG'CG_CC
Cuts at: 0 1261 1606 1682 1849 2258 2406 2842 2896
Size: 1261 345 76 167 409 148 436 54
Cuts at: 2896 3018
Size: 122
Fragments arranged by size:
1261 436 409 345 167 148 122 76
54
NcoI C'CATG_G
Cuts at: 0 354 3018
Size: 354 2664
NgoMIV G'CCGG_C
Cuts at: 0 1655 1684 2175 2254 2656 2682 2838 2892
Size: 1655 29 491 79 402 26 156 54
Cuts at: 2892 3018
Size: 126
Fragments arranged by size:
1655 491 402 156 126 79 54 29
26
NspI r_CATG'y
Cuts at: 0 933 3018
Size: 933 2085
PasI CC'CwG_GG
Cuts at: 0 2170 3018
Size: 2170 848
PmlI CAC'GTG
Cuts at: 0 629 3012 3018
Size: 629 2383 6
Fragments arranged by size:
2383 629 6
PpuMI rG'GwC_Cy
Cuts at: 0 2164 2601 2738 3018
Size: 2164 437 137 280
Fragments arranged by size:
2164 437 280 137
PspOMI G'GGCC_C
Cuts at: 0 311 345 1428 1444 1565 1594 1715 1917
Size: 311 34 1083 16 121 29 121 202
Cuts at: 1917 3018
Size: 1101
Fragments arranged by size:
1101 1083 311 202 121 121 34 29
16
PspXI vC'TCGA_Gb
Cuts at: 0 2485 3018
Size: 2485 533
PstI C_TGCA'G
Cuts at: 0 770 1664 3018
Size: 770 894 1354
Fragments arranged by size:
1354 894 770
PvuII CAG'CTG
Cuts at: 0 1055 3018
Size: 1055 1963
SacII CC_GC'GG
Cuts at: 0 1247 2308 2369 2404 2694 3018
Size: 1247 1061 61 35 290 324
Fragments arranged by size:
1247 1061 324 290 61 35
SanDI GG'GwC_CC
Cuts at: 0 2164 2738 3018
Size: 2164 574 280
Fragments arranged by size:
2164 574 280
SapI GCTCTTCn'nnn_
Cuts at: 0 233 1711 3018
Size: 233 1478 1307
Fragments arranged by size:
1478 1307 233
SbfI CC_TGCA'GG
Cuts at: 0 770 3018
Size: 770 2248
ScaI AGT'ACT
Cuts at: 0 2512 3018
Size: 2512 506
SfcI C'TryA_G
Cuts at: 0 766 1089 1660 3018
Size: 766 323 571 1358
Fragments arranged by size:
1358 766 571 323
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 679 3018
Size: 679 2339
SfoI GGC'GCC
Cuts at: 0 1262 1607 1683 1850 2259 2407 2843 2897
Size: 1262 345 76 167 409 148 436 54
Cuts at: 2897 3018
Size: 121
Fragments arranged by size:
1262 436 409 345 167 148 121 76
54
SgrAI Cr'CCGG_yG
Cuts at: 0 1684 2430 2838 2892 3018
Size: 1684 746 408 54 126
Fragments arranged by size:
1684 746 408 126 54
SmaI CCC'GGG
Cuts at: 0 1712 1916 2006 2162 2663 2736 2752 3018
Size: 1712 204 90 156 501 73 16 266
Fragments arranged by size:
1712 501 266 204 156 90 73 16
SmlI C'TyrA_G
Cuts at: 0 1015 2485 3018
Size: 1015 1470 533
Fragments arranged by size:
1470 1015 533
StuI AGG'CCT
Cuts at: 0 436 2059 2491 3018
Size: 436 1623 432 527
Fragments arranged by size:
1623 527 436 432
StyI C'CwwG_G
Cuts at: 0 194 354 992 1133 1721 1844 3018
Size: 194 160 638 141 588 123 1174
Fragments arranged by size:
1174 638 588 194 160 141 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 1181 3018
Size: 73 1108 1837
Fragments arranged by size:
1837 1108 73
TatI w'GTAC_w
Cuts at: 0 2510 3018
Size: 2510 508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 635 667 3018
Size: 635 32 2351
Fragments arranged by size:
2351 635 32
Tth111I GACn'n_nGTC
Cuts at: 0 1467 3018
Size: 1467 1551
XhoI C'TCGA_G
Cuts at: 0 2485 3018
Size: 2485 533
XmaI C'CCGG_G
Cuts at: 0 1710 1914 2004 2160 2661 2734 2750 3018
Size: 1710 204 90 156 501 73 16 268
Fragments arranged by size:
1710 501 268 204 156 90 73 16
ZraI GAC'GTC
Cuts at: 0 717 3018
Size: 717 2301
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AscI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FseI FspI
FspAI HaeII Hin4I HincII HpaI KasI KpnI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PmlI PpuMI PspOMI PspXI PstI PvuII SacII SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XhoI
XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AhdI AloI ApoI AseI
AsiSI AvrII BamHI BcgI BciVI BfrBI BmgBI BmtI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BstBI BstZ17I Bsu36I ClaI DraI EcoICRI EcoRI EcoRV
FalI HindIII MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PvuI RsrII SacI SalI SexAI
SgfI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XcmI XmnI