Restriction map for
pFN21AB9739
(Linear) (Six-base) MAPSORT of: KIBB9739.seq Check: 3084 from: 1 to: 1521
KIBB9739.orf
With 182 enzymes: SgfI *
March 4, 2011 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1033 1521
Size: 1033 488
AatII G_ACGT'C
Cuts at: 0 892 1521
Size: 892 629
Acc65I G'GTAC_C
Cuts at: 0 399 1521
Size: 399 1122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1034 1521
Size: 21 1013 487
Fragments arranged by size:
1013 487 21
AgeI A'CCGG_T
Cuts at: 0 1346 1521
Size: 1346 175
AleI CACnn'nnGTG
Cuts at: 0 262 958 1521
Size: 262 696 563
Fragments arranged by size:
696 563 262
ApaI G_GGCC'C
Cuts at: 0 1063 1521
Size: 1063 458
ApoI r'AATT_y
Cuts at: 0 1413 1479 1521
Size: 1413 66 42
Fragments arranged by size:
1413 66 42
AvaI C'yCGr_G
Cuts at: 0 629 1521
Size: 629 892
AvrII C'CTAG_G
Cuts at: 0 964 1521
Size: 964 557
BamHI G'GATC_C
Cuts at: 0 1312 1521
Size: 1312 209
BanI G'GyrC_C
Cuts at: 0 399 1332 1521
Size: 399 933 189
Fragments arranged by size:
933 399 189
BanII G_rGCy'C
Cuts at: 0 88 461 1063 1521
Size: 88 373 602 458
Fragments arranged by size:
602 458 373 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 558 827 914 1521
Size: 558 269 87 607
Fragments arranged by size:
607 558 269 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1167 1201 1521
Size: 1167 34 320
Fragments arranged by size:
1167 320 34
BlpI GC'TnA_GC
Cuts at: 0 455 1521
Size: 455 1066
Bme1580I G_kGCm'C
Cuts at: 0 230 937 1063 1521
Size: 230 707 126 458
Fragments arranged by size:
707 458 230 126
BmgBI CAC'GTC
Cuts at: 0 81 1521
Size: 81 1440
BmrI ACTGGGnnnn_n'
Cuts at: 0 716 947 1066 1521
Size: 716 231 119 455
Fragments arranged by size:
716 455 231 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 1521
Size: 33 1488
Bpu10I CC'TnA_GC
Cuts at: 0 877 1521
Size: 877 644
BsaI GGTCTCn'nnnn_
Cuts at: 0 1337 1355 1521
Size: 1337 18 166
Fragments arranged by size:
1337 166 18
BsaHI Gr'CG_yC
Cuts at: 0 889 1521
Size: 889 632
BsaWI w'CCGG_w
Cuts at: 0 461 1346 1521
Size: 461 885 175
Fragments arranged by size:
885 461 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 561 591 1521
Size: 561 30 930
Fragments arranged by size:
930 561 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1521
Size: 758 763
BsiEI CG_ry'CG
Cuts at: 0 241 290 1521
Size: 241 49 1231
Fragments arranged by size:
1231 241 49
BsiHKAI G_wGCw'C
Cuts at: 0 25 328 461 1521
Size: 25 303 133 1060
Fragments arranged by size:
1060 303 133 25
BsmI GAATG_Cn'
Cuts at: 0 471 710 1136 1367 1521
Size: 471 239 426 231 154
Fragments arranged by size:
471 426 239 231 154
Bsp1286I G_dGCh'C
Cuts at: 0 25 88 230 328 461 937 1063 1521
Size: 25 63 142 98 133 476 126 458
Fragments arranged by size:
476 458 142 133 126 98 63 25
BspEI T'CCGG_A
Cuts at: 0 461 1521
Size: 461 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1033 1229 1521
Size: 1033 196 292
Fragments arranged by size:
1033 292 196
BsrFI r'CCGG_y
Cuts at: 0 29 1346 1521
Size: 29 1317 175
Fragments arranged by size:
1317 175 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1432 1521
Size: 1432 89
BstYI r'GATC_y
Cuts at: 0 15 1312 1521
Size: 15 1297 209
Fragments arranged by size:
1297 209 15
Bsu36I CC'TnA_GG
Cuts at: 0 1173 1521
Size: 1173 348
BtgI C'CryG_G
Cuts at: 0 260 368 754 844 1328 1521
Size: 260 108 386 90 484 193
Fragments arranged by size:
484 386 260 193 108 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 1521
Size: 258 1263
BtsI GCAGTG_nn'
Cuts at: 0 230 986 1521
Size: 230 756 535
Fragments arranged by size:
756 535 230
DrdI GACnn_nn'nnGTC
Cuts at: 0 894 1521
Size: 894 627
EaeI y'GGCC_r
Cuts at: 0 238 287 1521
Size: 238 49 1234
Fragments arranged by size:
1234 238 49
EagI C'GGCC_G
Cuts at: 0 238 287 1521
Size: 238 49 1234
Fragments arranged by size:
1234 238 49
EarI CTCTTCn'nnn_
Cuts at: 0 1027 1248 1521
Size: 1027 221 273
Fragments arranged by size:
1027 273 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1185 1358 1521
Size: 1185 173 163
Fragments arranged by size:
1185 173 163
EcoICRI GAG'CTC
Cuts at: 0 459 1521
Size: 459 1062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 33 1034 1521
Size: 21 12 1001 487
Fragments arranged by size:
1001 487 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 1521
Size: 394 1127
EcoO109I rG'GnC_Cy
Cuts at: 0 1060 1521
Size: 1060 461
EcoRI G'AATT_C
Cuts at: 0 1479 1521
Size: 1479 42
FspI TGC'GCA
Cuts at: 0 101 1521
Size: 101 1420
HaeII r_GCGC'y
Cuts at: 0 36 351 1521
Size: 36 315 1170
Fragments arranged by size:
1170 315 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 722 754 1521
Size: 722 32 767
Fragments arranged by size:
767 722 32
HincII GTy'rAC
Cuts at: 0 1440 1521
Size: 1440 81
HindIII A'AGCT_T
Cuts at: 0 655 1521
Size: 655 866
KpnI G_GTAC'C
Cuts at: 0 403 1521
Size: 403 1118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1145 1521
Size: 1145 376
MslI CAynn'nnrTG
Cuts at: 0 262 958 1521
Size: 262 696 563
Fragments arranged by size:
696 563 262
MspA1I CmG'CkG
Cuts at: 0 220 918 1521
Size: 220 698 603
Fragments arranged by size:
698 603 220
NaeI GCC'GGC
Cuts at: 0 31 1521
Size: 31 1490
NcoI C'CATG_G
Cuts at: 0 260 1328 1521
Size: 260 1068 193
Fragments arranged by size:
1068 260 193
NgoMIV G'CCGG_C
Cuts at: 0 29 1521
Size: 29 1492
NotI GC'GGCC_GC
Cuts at: 0 238 1521
Size: 238 1283
NspI r_CATG'y
Cuts at: 0 1182 1521
Size: 1182 339
PspOMI G'GGCC_C
Cuts at: 0 1059 1521
Size: 1059 462
PstI C_TGCA'G
Cuts at: 0 62 225 1521
Size: 62 163 1296
Fragments arranged by size:
1296 163 62
PvuII CAG'CTG
Cuts at: 0 220 1521
Size: 220 1301
SacI G_AGCT'C
Cuts at: 0 461 1521
Size: 461 1060
SapI GCTCTTCn'nnn_
Cuts at: 0 1027 1248 1521
Size: 1027 221 273
Fragments arranged by size:
1027 273 221
SfcI C'TryA_G
Cuts at: 0 58 221 1521
Size: 58 163 1300
Fragments arranged by size:
1300 163 58
StyI C'CwwG_G
Cuts at: 0 260 964 1328 1521
Size: 260 704 364 193
Fragments arranged by size:
704 364 260 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 561 593 927 959 1447 1479 1521
Size: 561 32 334 32 488 32 42
Fragments arranged by size:
561 488 334 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 803 1521
Size: 803 718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1255 1521
Size: 1255 266
ZraI GAC'GTC
Cuts at: 0 890 1521
Size: 890 631
Enzymes that do cut:
AarI AatII Acc65I AcuI AgeI AleI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbsI BcgI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrFI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII KpnI MmeI
MslI MspA1I NaeI NcoI NgoMIV NotI NspI PspOMI
PstI PvuII SacI SapI SfcI StyI TstI Tth111I
XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI
BciVI BclI BfrBI BglI BglII BmtI BplI BpuEI
BsaAI BsaBI BseRI BseYI BsiWI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
ClaI CspCI DraI DraIII EcoRV FalI FseI FspAI
HpaI KasI MfeI MluI MscI NarI NdeI NheI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI XbaI XhoI
XmaI XmnI