Restriction map for
pFN21AB9733
(Linear) (Six-base) MAPSORT of: KIBB9733.seq Check: 1941 from: 1 to: 1449
KIBB9733.orf
With 182 enzymes: SgfI *
March 22, 2012 15:46 ..
Acc65I G'GTAC_C
Cuts at: 0 293 1449
Size: 293 1156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1449
Size: 679 770
AgeI A'CCGG_T
Cuts at: 0 1297 1449
Size: 1297 152
AleI CACnn'nnGTG
Cuts at: 0 475 1449
Size: 475 974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1449
Size: 1231 32 186
Fragments arranged by size:
1231 186 32
ApaI G_GGCC'C
Cuts at: 0 92 646 799 818 941 1094 1280 1449
Size: 92 554 153 19 123 153 186 169
Fragments arranged by size:
554 186 169 153 153 123 92 19
AvaI C'yCGr_G
Cuts at: 0 619 637 1087 1272 1279 1449
Size: 619 18 450 185 7 170
Fragments arranged by size:
619 450 185 170 18 7
AvrII C'CTAG_G
Cuts at: 0 1110 1449
Size: 1110 339
BanI G'GyrC_C
Cuts at: 0 165 202 293 309 506 888 918 1140
Size: 165 37 91 16 197 382 30 222
Cuts at: 1140 1449
Size: 309
Fragments arranged by size:
382 309 222 197 165 91 37 30
16
BanII G_rGCy'C
Cuts at: 0 92 107 127 253 286 611 646 799
Size: 92 15 20 126 33 325 35 153
Cuts at: 799 818 941 1069 1094 1280 1449
Size: 19 123 128 25 186 169
Fragments arranged by size:
325 186 169 153 128 126 123 92
35 33 25 20 19 15
BbeI G_GCGC'C
Cuts at: 0 169 206 313 510 892 1144 1449
Size: 169 37 107 197 382 252 305
Fragments arranged by size:
382 305 252 197 169 107 37
BbvCI CC'TCA_GC
Cuts at: 0 739 1449
Size: 739 710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1359 1393 1449
Size: 1359 34 56
Fragments arranged by size:
1359 56 34
BglI GCCn_nnn'nGGC
Cuts at: 0 43 163 1449
Size: 43 120 1286
Fragments arranged by size:
1286 120 43
BlpI GC'TnA_GC
Cuts at: 0 662 1449
Size: 662 787
Bme1580I G_kGCm'C
Cuts at: 0 61 92 646 799 818 941 1094 1280
Size: 61 31 554 153 19 123 153 186
Cuts at: 1280 1449
Size: 169
Fragments arranged by size:
554 186 169 153 153 123 61 31
19
BmgBI CAC'GTC
Cuts at: 0 533 1449
Size: 533 916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 783 1406 1449
Size: 783 623 43
Fragments arranged by size:
783 623 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 607 1449
Size: 178 429 842
Fragments arranged by size:
842 429 178
Bpu10I CC'TnA_GC
Cuts at: 0 739 1449
Size: 739 710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 496 1449
Size: 367 129 953
Fragments arranged by size:
953 367 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 1313 1449
Size: 1313 136
BsaAI yAC'GTr
Cuts at: 0 246 1449
Size: 246 1203
BsaHI Gr'CG_yC
Cuts at: 0 166 203 310 368 507 538 763 889
Size: 166 37 107 58 139 31 225 126
Cuts at: 889 1126 1141 1449
Size: 237 15 308
Fragments arranged by size:
308 237 225 166 139 126 107 58
37 31 15
BsaWI w'CCGG_w
Cuts at: 0 347 1297 1449
Size: 347 950 152
Fragments arranged by size:
950 347 152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1231 1261 1449
Size: 1231 30 188
Fragments arranged by size:
1231 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 583 586 671 1449
Size: 228 355 3 85 778
Fragments arranged by size:
778 355 228 85 3
BseYI C'CCAG_C
Cuts at: 0 339 826 1449
Size: 339 487 623
Fragments arranged by size:
623 487 339
BsiEI CG_ry'CG
Cuts at: 0 111 218 1449
Size: 111 107 1231
Fragments arranged by size:
1231 111 107
BsiHKAI G_wGCw'C
Cuts at: 0 107 404 986 1449
Size: 107 297 582 463
Fragments arranged by size:
582 463 297 107
BsiWI C'GTAC_G
Cuts at: 0 242 1449
Size: 242 1207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 718 754 1449
Size: 718 36 695
Fragments arranged by size:
718 695 36
Bsp1286I G_dGCh'C
Cuts at: 0 61 92 107 127 253 286 404 611
Size: 61 31 15 20 126 33 118 207
Cuts at: 611 646 799 818 941 986 1069 1094 1280
Size: 35 153 19 123 45 83 25 186
Cuts at: 1280 1449
Size: 169
Fragments arranged by size:
207 186 169 153 126 123 118 83
61 45 35 33 31 25 20 19
15
BsrBI CCG'CTC
Cuts at: 0 113 1348 1449
Size: 113 1235 101
Fragments arranged by size:
1235 113 101
BsrFI r'CCGG_y
Cuts at: 0 503 808 885 1131 1297 1449
Size: 503 305 77 246 166 152
Fragments arranged by size:
503 305 246 166 152 77
BssHII G'CGCG_C
Cuts at: 0 499 1449
Size: 499 950
BssSI C'ACGA_G
Cuts at: 0 54 1449
Size: 54 1395
BtgI C'CryG_G
Cuts at: 0 212 371 541 908 1449
Size: 212 159 170 367 541
Fragments arranged by size:
541 367 212 170 159
BtsI GCAGTG_nn'
Cuts at: 0 1171 1449
Size: 1171 278
DrdI GACnn_nn'nnGTC
Cuts at: 0 1191 1449
Size: 1191 258
EaeI y'GGCC_r
Cuts at: 0 108 215 446 1266 1449
Size: 108 107 231 820 183
Fragments arranged by size:
820 231 183 108 107
EagI C'GGCC_G
Cuts at: 0 108 215 1449
Size: 108 107 1234
Fragments arranged by size:
1234 108 107
EarI CTCTTCn'nnn_
Cuts at: 0 120 560 1449
Size: 120 440 889
Fragments arranged by size:
889 440 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 865 953 1449
Size: 24 841 88 496
Fragments arranged by size:
841 496 88 24
EcoICRI GAG'CTC
Cuts at: 0 105 1449
Size: 105 1344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 607 679 1449
Size: 178 429 72 770
Fragments arranged by size:
770 429 178 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 1175 1449
Size: 472 703 274
Fragments arranged by size:
703 472 274
EcoO109I rG'GnC_Cy
Cuts at: 0 88 89 336 642 643 795 796 815
Size: 88 1 247 306 1 152 1 19
Cuts at: 815 855 928 938 1276 1288 1449
Size: 40 73 10 338 12 161
Fragments arranged by size:
338 306 247 161 152 88 73 40
19 12 10 1 1 1
HaeII r_GCGC'y
Cuts at: 0 49 169 206 313 510 886 892 1144
Size: 49 120 37 107 197 376 6 252
Cuts at: 1144 1449
Size: 305
Fragments arranged by size:
376 305 252 197 120 107 49 37
6
KasI G'GCGC_C
Cuts at: 0 165 202 309 506 888 1140 1449
Size: 165 37 107 197 382 252 309
Fragments arranged by size:
382 309 252 197 165 107 37
KpnI G_GTAC'C
Cuts at: 0 297 1449
Size: 297 1152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1449
Size: 1010 439
MscI TGG'CCA
Cuts at: 0 448 1268 1449
Size: 448 820 181
Fragments arranged by size:
820 448 181
MslI CAynn'nnrTG
Cuts at: 0 460 475 1262 1316 1449
Size: 460 15 787 54 133
Fragments arranged by size:
787 460 133 54 15
MspA1I CmG'CkG
Cuts at: 0 214 543 835 910 1449
Size: 214 329 292 75 539
Fragments arranged by size:
539 329 292 214 75
NaeI GCC'GGC
Cuts at: 0 505 887 1133 1449
Size: 505 382 246 316
Fragments arranged by size:
505 382 316 246
NarI GG'CG_CC
Cuts at: 0 166 203 310 507 889 1141 1449
Size: 166 37 107 197 382 252 308
Fragments arranged by size:
382 308 252 197 166 107 37
NgoMIV G'CCGG_C
Cuts at: 0 503 885 1131 1449
Size: 503 382 246 318
Fragments arranged by size:
503 382 318 246
NotI GC'GGCC_GC
Cuts at: 0 215 1449
Size: 215 1234
PasI CC'CwG_GG
Cuts at: 0 315 1449
Size: 315 1134
PflMI CCAn_nnn'nTGG
Cuts at: 0 1001 1449
Size: 1001 448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1231 1263 1449
Size: 1051 32 148 32 186
Fragments arranged by size:
1051 186 148 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 336 855 928 1449
Size: 336 519 73 521
Fragments arranged by size:
521 519 336 73
PspOMI G'GGCC_C
Cuts at: 0 88 642 795 814 937 1090 1276 1449
Size: 88 554 153 19 123 153 186 173
Fragments arranged by size:
554 186 173 153 153 123 88 19
PstI C_TGCA'G
Cuts at: 0 717 794 1449
Size: 717 77 655
Fragments arranged by size:
717 655 77
RsrII CG'GwC_CG
Cuts at: 0 1300 1449
Size: 1300 149
SacI G_AGCT'C
Cuts at: 0 107 1449
Size: 107 1342
SacII CC_GC'GG
Cuts at: 0 215 544 911 1449
Size: 215 329 367 538
Fragments arranged by size:
538 367 329 215
SanDI GG'GwC_CC
Cuts at: 0 336 928 1449
Size: 336 592 521
Fragments arranged by size:
592 521 336
SapI GCTCTTCn'nnn_
Cuts at: 0 120 1449
Size: 120 1329
SbfI CC_TGCA'GG
Cuts at: 0 717 1449
Size: 717 732
SexAI A'CCwGG_T
Cuts at: 0 484 1449
Size: 484 965
SfcI C'TryA_G
Cuts at: 0 713 790 1449
Size: 713 77 659
Fragments arranged by size:
713 659 77
SfoI GGC'GCC
Cuts at: 0 167 204 311 508 890 1142 1449
Size: 167 37 107 197 382 252 307
Fragments arranged by size:
382 307 252 197 167 107 37
SgrAI Cr'CCGG_yG
Cuts at: 0 503 808 885 1449
Size: 503 305 77 564
Fragments arranged by size:
564 503 305 77
SmaI CCC'GGG
Cuts at: 0 1089 1274 1281 1449
Size: 1089 185 7 168
Fragments arranged by size:
1089 185 168 7
SmlI C'TyrA_G
Cuts at: 0 382 511 1449
Size: 382 129 938
Fragments arranged by size:
938 382 129
SrfI GCCC'GGGC
Cuts at: 0 1281 1449
Size: 1281 168
StuI AGG'CCT
Cuts at: 0 388 1449
Size: 388 1061
StyI C'CwwG_G
Cuts at: 0 287 425 1110 1449
Size: 287 138 685 339
Fragments arranged by size:
685 339 287 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 487 720 1449
Size: 487 233 729
Fragments arranged by size:
729 487 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1284 1316 1449
Size: 1284 32 133
Fragments arranged by size:
1284 133 32
Tth111I GACn'n_nGTC
Cuts at: 0 1036 1449
Size: 1036 413
XmaI C'CCGG_G
Cuts at: 0 1087 1272 1279 1449
Size: 1087 185 7 170
Fragments arranged by size:
1087 185 170 7
XmnI GAAnn'nnTTC
Cuts at: 0 692 1449
Size: 692 757
Enzymes that do cut:
Acc65I AcuI AgeI AleI AloI ApaI AvaI AvrII
BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BsrBI BsrFI BssHII BssSI BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI PasI PflMI PpiI PpuMI PspOMI
PstI RsrII SacI SacII SanDI SapI SbfI SexAI
SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AlfI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbsI BciVI BclI BfrBI BglII BmrI BmtI
BsaBI BsgI BsmI BspEI BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
NcoI NdeI NheI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
PvuII SalI ScaI SfiI SgfI SnaBI SpeI SphI
SspI SwaI TatI XbaI XcmI XhoI ZraI