Restriction map for
pFN21AB9721
(Linear) (Six-base) MAPSORT of: KIBB9721.seq Check: 9373 from: 1 to: 1221
KIBB9721.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 836 1221
Size: 836 385
AccI GT'mk_AC
Cuts at: 0 302 1221
Size: 302 919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 882 1221
Size: 882 339
AflIII A'CryG_T
Cuts at: 0 923 1221
Size: 923 298
AhdI GACnn_n'nnGTC
Cuts at: 0 345 1221
Size: 345 876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 118 1221
Size: 118 1103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 607 639 1221
Size: 607 32 582
Fragments arranged by size:
607 582 32
AlwNI CAG_nnn'CTG
Cuts at: 0 726 1221
Size: 726 495
ApaI G_GGCC'C
Cuts at: 0 1004 1221
Size: 1004 217
ApoI r'AATT_y
Cuts at: 0 75 601 1221
Size: 75 526 620
Fragments arranged by size:
620 526 75
AvaI C'yCGr_G
Cuts at: 0 115 149 170 572 997 1085 1221
Size: 115 34 21 402 425 88 136
Fragments arranged by size:
425 402 136 115 88 34 21
BanI G'GyrC_C
Cuts at: 0 126 241 517 862 1018 1080 1206 1221
Size: 126 115 276 345 156 62 126 15
Fragments arranged by size:
345 276 156 126 126 115 62 15
BanII G_rGCy'C
Cuts at: 0 148 251 1004 1221
Size: 148 103 753 217
Fragments arranged by size:
753 217 148 103
BbeI G_GCGC'C
Cuts at: 0 245 866 1022 1084 1221
Size: 245 621 156 62 137
Fragments arranged by size:
621 245 156 137 62
BciVI GTATCCnnnnn_n'
Cuts at: 0 633 1221
Size: 633 588
BlpI GC'TnA_GC
Cuts at: 0 1165 1221
Size: 1165 56
Bme1580I G_kGCm'C
Cuts at: 0 131 1004 1211 1221
Size: 131 873 207 10
Fragments arranged by size:
873 207 131 10
BmgBI CAC'GTC
Cuts at: 0 737 1221
Size: 737 484
BmrI ACTGGGnnnn_n'
Cuts at: 0 323 1024 1221
Size: 323 701 197
Fragments arranged by size:
701 323 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1221
Size: 613 608
Bpu10I CC'TnA_GC
Cuts at: 0 142 1221
Size: 142 1079
BsaI GGTCTCn'nnnn_
Cuts at: 0 466 1221
Size: 466 755
BsaAI yAC'GTr
Cuts at: 0 621 1221
Size: 621 600
BsaHI Gr'CG_yC
Cuts at: 0 242 863 1019 1081 1221
Size: 242 621 156 62 140
Fragments arranged by size:
621 242 156 140 62
BsaWI w'CCGG_w
Cuts at: 0 313 1221
Size: 313 908
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 1221
Size: 295 30 896
Fragments arranged by size:
896 295 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 214 1167 1216 1221
Size: 211 3 953 49 5
Fragments arranged by size:
953 211 49 5 3
BseYI C'CCAG_C
Cuts at: 0 259 1221
Size: 259 962
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 549 1221
Size: 363 186 672
Fragments arranged by size:
672 363 186
BsiEI CG_ry'CG
Cuts at: 0 653 794 1221
Size: 653 141 427
Fragments arranged by size:
653 427 141
BsiHKAI G_wGCw'C
Cuts at: 0 1166 1221
Size: 1166 55
Bsp1286I G_dGCh'C
Cuts at: 0 131 148 251 1004 1166 1211 1221
Size: 131 17 103 753 162 45 10
Fragments arranged by size:
753 162 131 103 45 17 10
BspEI T'CCGG_A
Cuts at: 0 313 1221
Size: 313 908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 836 1221
Size: 671 165 385
Fragments arranged by size:
671 385 165
BsrBI CCG'CTC
Cuts at: 0 918 990 1130 1221
Size: 918 72 140 91
Fragments arranged by size:
918 140 91 72
BsrFI r'CCGG_y
Cuts at: 0 204 698 849 1027 1221
Size: 204 494 151 178 194
Fragments arranged by size:
494 204 194 178 151
BstXI CCAn_nnnn'nTGG
Cuts at: 0 259 1077 1221
Size: 259 818 144
Fragments arranged by size:
818 259 144
BtgI C'CryG_G
Cuts at: 0 89 132 631 794 952 1221
Size: 89 43 499 163 158 269
Fragments arranged by size:
499 269 163 158 89 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 806 1221
Size: 806 415
BtsI GCAGTG_nn'
Cuts at: 0 501 1221
Size: 501 720
EaeI y'GGCC_r
Cuts at: 0 164 266 650 728 739 791 1067 1221
Size: 164 102 384 78 11 52 276 154
Fragments arranged by size:
384 276 164 154 102 78 52 11
EagI C'GGCC_G
Cuts at: 0 650 791 1221
Size: 650 141 430
Fragments arranged by size:
650 430 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 766 861 870 967 1221
Size: 766 95 9 97 254
Fragments arranged by size:
766 254 97 95 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 882 1221
Size: 613 269 339
Fragments arranged by size:
613 339 269
EcoO109I rG'GnC_Cy
Cuts at: 0 283 1221
Size: 283 938
EcoRI G'AATT_C
Cuts at: 0 601 1221
Size: 601 620
HaeII r_GCGC'y
Cuts at: 0 245 445 536 726 866 1022 1084 1221
Size: 245 200 91 190 140 156 62 137
Fragments arranged by size:
245 200 190 156 140 137 91 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 331 363 1221
Size: 295 32 4 32 858
Fragments arranged by size:
858 295 32 32 4
HincII GTy'rAC
Cuts at: 0 303 1221
Size: 303 918
KasI G'GCGC_C
Cuts at: 0 241 862 1018 1080 1221
Size: 241 621 156 62 141
Fragments arranged by size:
621 241 156 141 62
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1221
Size: 329 892
MscI TGG'CCA
Cuts at: 0 730 1221
Size: 730 491
MslI CAynn'nnrTG
Cuts at: 0 22 682 772 1221
Size: 22 660 90 449
Fragments arranged by size:
660 449 90 22
MspA1I CmG'CkG
Cuts at: 0 102 633 796 902 954 1061 1221
Size: 102 531 163 106 52 107 160
Fragments arranged by size:
531 163 160 107 106 102 52
NaeI GCC'GGC
Cuts at: 0 1029 1221
Size: 1029 192
NarI GG'CG_CC
Cuts at: 0 242 863 1019 1081 1221
Size: 242 621 156 62 140
Fragments arranged by size:
621 242 156 140 62
NgoMIV G'CCGG_C
Cuts at: 0 1027 1221
Size: 1027 194
NotI GC'GGCC_GC
Cuts at: 0 650 791 1221
Size: 650 141 430
Fragments arranged by size:
650 430 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 737 1221
Size: 737 484
PpuMI rG'GwC_Cy
Cuts at: 0 283 1221
Size: 283 938
PspOMI G'GGCC_C
Cuts at: 0 1000 1221
Size: 1000 221
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 394 426 1221
Size: 394 32 795
Fragments arranged by size:
795 394 32
SacII CC_GC'GG
Cuts at: 0 634 797 955 1221
Size: 634 163 158 266
Fragments arranged by size:
634 266 163 158
SalI G'TCGA_C
Cuts at: 0 301 1221
Size: 301 920
SfoI GGC'GCC
Cuts at: 0 243 864 1020 1082 1221
Size: 243 621 156 62 139
Fragments arranged by size:
621 243 156 139 62
SgrAI Cr'CCGG_yG
Cuts at: 0 849 1221
Size: 849 372
SmaI CCC'GGG
Cuts at: 0 172 999 1087 1221
Size: 172 827 88 134
Fragments arranged by size:
827 172 134 88
SnaBI TAC'GTA
Cuts at: 0 621 1221
Size: 621 600
StuI AGG'CCT
Cuts at: 0 1174 1221
Size: 1174 47
XmaI C'CCGG_G
Cuts at: 0 170 997 1085 1221
Size: 170 827 88 136
Fragments arranged by size:
827 170 136 88
XmnI GAAnn'nnTTC
Cuts at: 0 601 1221
Size: 601 620
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BciVI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrFI BstXI BtgI BtgZI BtsI
EaeI EagI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI PflMI PpuMI PspOMI PsrI SacII SalI
SfoI SgrAI SmaI SnaBI StuI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BclI BfrBI BglI BglII BmtI BplI BpuEI
BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PstI PvuI PvuII RsrII SacI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SmlI SpeI
SphI SrfI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI ZraI