Restriction map for
pFN21AB9713
(Linear) (Six-base) MAPSORT of: KIBB9713.seq Check: 3564 from: 1 to: 1122
KIBB9713.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1089 1122
Size: 1089 33
AatII G_ACGT'C
Cuts at: 0 65 1122
Size: 65 1057
AfeI AGC'GCT
Cuts at: 0 954 1122
Size: 954 168
AlwNI CAG_nnn'CTG
Cuts at: 0 99 243 1122
Size: 99 144 879
Fragments arranged by size:
879 144 99
ApoI r'AATT_y
Cuts at: 0 645 1122
Size: 645 477
AvaI C'yCGr_G
Cuts at: 0 286 1122
Size: 286 836
BanI G'GyrC_C
Cuts at: 0 44 535 1122
Size: 44 491 587
Fragments arranged by size:
587 491 44
BbeI G_GCGC'C
Cuts at: 0 48 539 1122
Size: 48 491 583
Fragments arranged by size:
583 491 48
BbvCI CC'TCA_GC
Cuts at: 0 269 1122
Size: 269 853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 651 685 1122
Size: 651 34 437
Fragments arranged by size:
651 437 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 858 1122
Size: 858 264
BmgBI CAC'GTC
Cuts at: 0 465 1122
Size: 465 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 979 1092 1106 1122
Size: 463 516 113 14 16
Fragments arranged by size:
516 463 113 16 14
Bpu10I CC'TnA_GC
Cuts at: 0 269 1122
Size: 269 853
BsaI GGTCTCn'nnnn_
Cuts at: 0 774 1082 1122
Size: 774 308 40
Fragments arranged by size:
774 308 40
BsaHI Gr'CG_yC
Cuts at: 0 45 62 233 536 962 1122
Size: 45 17 171 303 426 160
Fragments arranged by size:
426 303 171 160 45 17
BsaWI w'CCGG_w
Cuts at: 0 797 1122
Size: 797 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 296 326 1122
Size: 296 30 796
Fragments arranged by size:
796 296 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 317 320 466 918 992 1122
Size: 317 3 146 452 74 130
Fragments arranged by size:
452 317 146 130 74 3
BseYI C'CCAG_C
Cuts at: 0 236 712 949 955 1122
Size: 236 476 237 6 167
Fragments arranged by size:
476 237 236 167 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1122
Size: 300 822
BsiHKAI G_wGCw'C
Cuts at: 0 170 371 586 1122
Size: 170 201 215 536
Fragments arranged by size:
536 215 201 170
BsmI GAATG_Cn'
Cuts at: 0 209 1122
Size: 209 913
Bsp1286I G_dGCh'C
Cuts at: 0 170 371 586 1122
Size: 170 201 215 536
Fragments arranged by size:
536 215 201 170
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1074 1089 1122
Size: 1074 15 33
Fragments arranged by size:
1074 33 15
BsrBI CCG'CTC
Cuts at: 0 310 1122
Size: 310 812
BsrFI r'CCGG_y
Cuts at: 0 322 1122
Size: 322 800
BssSI C'ACGA_G
Cuts at: 0 54 1122
Size: 54 1068
BstYI r'GATC_y
Cuts at: 0 222 398 1122
Size: 222 176 724
Fragments arranged by size:
724 222 176
Bsu36I CC'TnA_GG
Cuts at: 0 244 512 1122
Size: 244 268 610
Fragments arranged by size:
610 268 244
BtgI C'CryG_G
Cuts at: 0 40 204 869 1122
Size: 40 164 665 253
Fragments arranged by size:
665 253 164 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 11 1122
Size: 11 1111
BtsI GCAGTG_nn'
Cuts at: 0 602 1122
Size: 602 520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 858 893 1122
Size: 858 35 229
Fragments arranged by size:
858 229 35
DraIII CAC_nnn'GTG
Cuts at: 0 340 1122
Size: 340 782
EaeI y'GGCC_r
Cuts at: 0 2 80 314 737 1122
Size: 2 78 234 423 385
Fragments arranged by size:
423 385 234 78 2
EarI CTCTTCn'nnn_
Cuts at: 0 286 294 725 824 1122
Size: 286 8 431 99 298
Fragments arranged by size:
431 298 286 99 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 657 1122
Size: 657 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 979 1092 1106 1122
Size: 463 516 113 14 16
Fragments arranged by size:
516 463 113 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 478 1010 1122
Size: 103 375 532 112
Fragments arranged by size:
532 375 112 103
EcoO109I rG'GnC_Cy
Cuts at: 0 882 1122
Size: 882 240
HaeII r_GCGC'y
Cuts at: 0 48 539 560 956 1122
Size: 48 491 21 396 166
Fragments arranged by size:
491 396 166 48 21
HincII GTy'rAC
Cuts at: 0 911 1122
Size: 911 211
KasI G'GCGC_C
Cuts at: 0 44 535 1122
Size: 44 491 587
Fragments arranged by size:
587 491 44
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 1122
Size: 426 696
MscI TGG'CCA
Cuts at: 0 82 316 739 1122
Size: 82 234 423 383
Fragments arranged by size:
423 383 234 82
MslI CAynn'nnrTG
Cuts at: 0 565 1122
Size: 565 557
MspA1I CmG'CkG
Cuts at: 0 42 902 945 1122
Size: 42 860 43 177
Fragments arranged by size:
860 177 43 42
NaeI GCC'GGC
Cuts at: 0 324 1122
Size: 324 798
NarI GG'CG_CC
Cuts at: 0 45 536 1122
Size: 45 491 586
Fragments arranged by size:
586 491 45
NgoMIV G'CCGG_C
Cuts at: 0 322 1122
Size: 322 800
NspI r_CATG'y
Cuts at: 0 127 261 1122
Size: 127 134 861
Fragments arranged by size:
861 134 127
PasI CC'CwG_GG
Cuts at: 0 886 1035 1122
Size: 886 149 87
Fragments arranged by size:
886 149 87
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 1122
Size: 99 1023
PfoI T'CCnGG_A
Cuts at: 0 479 1122
Size: 479 643
PpuMI rG'GwC_Cy
Cuts at: 0 882 1122
Size: 882 240
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 691 723 1122
Size: 691 32 399
Fragments arranged by size:
691 399 32
PstI C_TGCA'G
Cuts at: 0 71 1025 1122
Size: 71 954 97
Fragments arranged by size:
954 97 71
PvuII CAG'CTG
Cuts at: 0 902 1122
Size: 902 220
SacII CC_GC'GG
Cuts at: 0 43 1122
Size: 43 1079
SanDI GG'GwC_CC
Cuts at: 0 882 1122
Size: 882 240
SapI GCTCTTCn'nnn_
Cuts at: 0 725 1122
Size: 725 397
SexAI A'CCwGG_T
Cuts at: 0 336 1122
Size: 336 786
SfcI C'TryA_G
Cuts at: 0 67 378 1021 1122
Size: 67 311 643 101
Fragments arranged by size:
643 311 101 67
SfoI GGC'GCC
Cuts at: 0 46 537 1122
Size: 46 491 585
Fragments arranged by size:
585 491 46
SphI G_CATG'C
Cuts at: 0 127 1122
Size: 127 995
StuI AGG'CCT
Cuts at: 0 442 501 1122
Size: 442 59 621
Fragments arranged by size:
621 442 59
TatI w'GTAC_w
Cuts at: 0 1111 1122
Size: 1111 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 296 328 1122
Size: 296 32 794
Fragments arranged by size:
794 296 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 1003 1122
Size: 732 271 119
Fragments arranged by size:
732 271 119
ZraI GAC'GTC
Cuts at: 0 63 1122
Size: 63 1059
Enzymes that do cut:
AarI AatII AfeI AlwNI ApoI AvaI BanI BbeI
BbvCI BcgI BciVI BmgBI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BssSI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PflMI PfoI PpuMI
PsrI PstI PvuII SacII SanDI SapI SexAI SfcI
SfoI SphI StuI TatI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbsI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaAI
BsaBI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI DraI
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SalI SbfI ScaI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StyI SwaI TaqII
Tth111I XbaI XhoI XmaI XmnI