Restriction map for
pFN21AB9708
(Linear) (Six-base) MAPSORT of: KIBB9708.seq Check: 1722 from: 1 to: 1023
KIBB9708.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 700 1023
Size: 306 394 323
Fragments arranged by size:
394 323 306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 1023
Size: 594 429
AlwNI CAG_nnn'CTG
Cuts at: 0 273 846 900 1023
Size: 273 573 54 123
Fragments arranged by size:
573 273 123 54
ApaI G_GGCC'C
Cuts at: 0 404 883 1023
Size: 404 479 140
Fragments arranged by size:
479 404 140
AvaI C'yCGr_G
Cuts at: 0 70 96 145 162 194 397 477 700
Size: 70 26 49 17 32 203 80 223
Cuts at: 700 727 784 1023
Size: 27 57 239
Fragments arranged by size:
239 223 203 80 70 57 49 32
27 26 17
BanI G'GyrC_C
Cuts at: 0 488 851 934 1023
Size: 488 363 83 89
Fragments arranged by size:
488 363 89 83
BanII G_rGCy'C
Cuts at: 0 169 404 883 1023
Size: 169 235 479 140
Fragments arranged by size:
479 235 169 140
BbeI G_GCGC'C
Cuts at: 0 492 938 1023
Size: 492 446 85
Fragments arranged by size:
492 446 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 764 1023
Size: 764 259
BglI GCCn_nnn'nGGC
Cuts at: 0 18 114 304 399 1023
Size: 18 96 190 95 624
Fragments arranged by size:
624 190 96 95 18
Bme1580I G_kGCm'C
Cuts at: 0 404 854 883 1023
Size: 404 450 29 140
Fragments arranged by size:
450 404 140 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1023
Size: 1021 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1023
Size: 207 816
BsaAI yAC'GTr
Cuts at: 0 918 1023
Size: 918 105
BsaHI Gr'CG_yC
Cuts at: 0 92 489 551 638 935 1023
Size: 92 397 62 87 297 88
Fragments arranged by size:
397 297 92 88 87 62
BsaWI w'CCGG_w
Cuts at: 0 419 1023
Size: 419 604
BseYI C'CCAG_C
Cuts at: 0 127 135 213 308 438 494 538 1023
Size: 127 8 78 95 130 56 44 485
Fragments arranged by size:
485 130 127 95 78 56 44 8
BsiEI CG_ry'CG
Cuts at: 0 236 299 579 680 780 809 989 1023
Size: 236 63 280 101 100 29 180 34
Fragments arranged by size:
280 236 180 101 100 63 34 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 83 193 1023
Size: 83 110 830
Fragments arranged by size:
830 110 83
Bsp1286I G_dGCh'C
Cuts at: 0 169 404 854 883 1023
Size: 169 235 450 29 140
Fragments arranged by size:
450 235 169 140 29
BspEI T'CCGG_A
Cuts at: 0 419 1023
Size: 419 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 306 700 1023
Size: 306 394 323
Fragments arranged by size:
394 323 306
BsrBI CCG'CTC
Cuts at: 0 30 473 1023
Size: 30 443 550
Fragments arranged by size:
550 443 30
BsrFI r'CCGG_y
Cuts at: 0 153 231 531 1023
Size: 153 78 300 492
Fragments arranged by size:
492 300 153 78
BssHII G'CGCG_C
Cuts at: 0 131 243 245 498 710 794 1023
Size: 131 112 2 253 212 84 229
Fragments arranged by size:
253 229 212 131 112 84 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 1023
Size: 318 705
BstXI CCAn_nnnn'nTGG
Cuts at: 0 135 1023
Size: 135 888
BstYI r'GATC_y
Cuts at: 0 416 1023
Size: 416 607
Bsu36I CC'TnA_GG
Cuts at: 0 1006 1023
Size: 1006 17
BtgI C'CryG_G
Cuts at: 0 76 720 962 1023
Size: 76 644 242 61
Fragments arranged by size:
644 242 76 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 849 1023
Size: 849 174
BtsI GCAGTG_nn'
Cuts at: 0 343 1023
Size: 343 680
EaeI y'GGCC_r
Cuts at: 0 233 296 387 777 806 959 986 1023
Size: 233 63 91 390 29 153 27 37
Fragments arranged by size:
390 233 153 91 63 37 29 27
EagI C'GGCC_G
Cuts at: 0 233 296 777 806 986 1023
Size: 233 63 481 29 180 37
Fragments arranged by size:
481 233 180 63 37 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 594 1023
Size: 207 387 429
Fragments arranged by size:
429 387 207
EcoO109I rG'GnC_Cy
Cuts at: 0 101 270 401 731 1002 1011 1023
Size: 101 169 131 330 271 9 12
Fragments arranged by size:
330 271 169 131 101 12 9
HaeII r_GCGC'y
Cuts at: 0 194 492 938 1023
Size: 194 298 446 85
Fragments arranged by size:
446 298 194 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 1023
Size: 751 32 240
Fragments arranged by size:
751 240 32
KasI G'GCGC_C
Cuts at: 0 488 934 1023
Size: 488 446 89
Fragments arranged by size:
488 446 89
MspA1I CmG'CkG
Cuts at: 0 78 561 722 885 897 927 1023
Size: 78 483 161 163 12 30 96
Fragments arranged by size:
483 163 161 96 78 30 12
NaeI GCC'GGC
Cuts at: 0 533 1023
Size: 533 490
NarI GG'CG_CC
Cuts at: 0 489 935 1023
Size: 489 446 88
Fragments arranged by size:
489 446 88
NgoMIV G'CCGG_C
Cuts at: 0 531 1023
Size: 531 492
NotI GC'GGCC_GC
Cuts at: 0 986 1023
Size: 986 37
NruI TCG'CGA
Cuts at: 0 377 868 1023
Size: 377 491 155
Fragments arranged by size:
491 377 155
PasI CC'CwG_GG
Cuts at: 0 846 1023
Size: 846 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 918 1023
Size: 918 105
PfoI T'CCnGG_A
Cuts at: 0 254 1023
Size: 254 769
PmlI CAC'GTG
Cuts at: 0 918 1023
Size: 918 105
PpuMI rG'GwC_Cy
Cuts at: 0 101 731 1002 1011 1023
Size: 101 630 271 9 12
Fragments arranged by size:
630 271 101 12 9
PspOMI G'GGCC_C
Cuts at: 0 400 879 1023
Size: 400 479 144
Fragments arranged by size:
479 400 144
PstI C_TGCA'G
Cuts at: 0 842 1023
Size: 842 181
PvuII CAG'CTG
Cuts at: 0 897 927 1023
Size: 897 30 96
Fragments arranged by size:
897 96 30
SacII CC_GC'GG
Cuts at: 0 79 723 1023
Size: 79 644 300
Fragments arranged by size:
644 300 79
SanDI GG'GwC_CC
Cuts at: 0 101 731 1011 1023
Size: 101 630 280 12
Fragments arranged by size:
630 280 101 12
SfcI C'TryA_G
Cuts at: 0 838 1023
Size: 838 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 18 304 1023
Size: 18 286 719
Fragments arranged by size:
719 286 18
SfoI GGC'GCC
Cuts at: 0 490 936 1023
Size: 490 446 87
Fragments arranged by size:
490 446 87
SmaI CCC'GGG
Cuts at: 0 98 164 196 399 479 729 1023
Size: 98 66 32 203 80 250 294
Fragments arranged by size:
294 250 203 98 80 66 32
SrfI GCCC'GGGC
Cuts at: 0 399 1023
Size: 399 624
Tth111I GACn'n_nGTC
Cuts at: 0 951 1023
Size: 951 72
XmaI C'CCGG_G
Cuts at: 0 96 162 194 397 477 727 1023
Size: 96 66 32 203 80 250 296
Fragments arranged by size:
296 250 203 96 80 66 32
Enzymes that do cut:
AarI AcuI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BglI Bme1580I BplI BpmI BsaAI BsaHI BsaWI
BseYI BsiEI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EagI Eco57MI EcoO109I HaeII Hin4I KasI MspA1I
NaeI NarI NgoMIV NotI NruI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacII SanDI SfcI
SfiI SfoI SmaI SrfI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaBI BsaXI BseRI BsgI BsiHKAI BsiWI BsmI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NsiI NspI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI XbaI XcmI XhoI XmnI ZraI