Restriction map for
pFN21AB9693
(Linear) (Six-base) MAPSORT of: KIBB9693.seq Check: 8256 from: 1 to: 756
KIBB9693.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 239 449 756
Size: 239 210 307
Fragments arranged by size:
307 239 210
AfeI AGC'GCT
Cuts at: 0 556 583 756
Size: 556 27 173
Fragments arranged by size:
556 173 27
ApaI G_GGCC'C
Cuts at: 0 109 208 756
Size: 109 99 548
Fragments arranged by size:
548 109 99
ApaLI G'TGCA_C
Cuts at: 0 73 756
Size: 73 683
AscI GG'CGCG_CC
Cuts at: 0 136 756
Size: 136 620
AvaI C'yCGr_G
Cuts at: 0 94 157 165 201 282 333 756
Size: 94 63 8 36 81 51 423
Fragments arranged by size:
423 94 81 63 51 36 8
BanI G'GyrC_C
Cuts at: 0 64 211 222 289 349 653 661 756
Size: 64 147 11 67 60 304 8 95
Fragments arranged by size:
304 147 95 67 64 60 11 8
BanII G_rGCy'C
Cuts at: 0 109 208 715 756
Size: 109 99 507 41
Fragments arranged by size:
507 109 99 41
BbeI G_GCGC'C
Cuts at: 0 68 215 226 293 353 657 756
Size: 68 147 11 67 60 304 99
Fragments arranged by size:
304 147 99 68 67 60 11
BbvCI CC'TCA_GC
Cuts at: 0 312 756
Size: 312 444
BglI GCCn_nnn'nGGC
Cuts at: 0 98 220 629 756
Size: 98 122 409 127
Fragments arranged by size:
409 127 122 98
Bme1580I G_kGCm'C
Cuts at: 0 77 109 208 666 756
Size: 77 32 99 458 90
Fragments arranged by size:
458 99 90 77 32
BmgBI CAC'GTC
Cuts at: 0 42 756
Size: 42 714
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 756
Size: 168 588
Bpu10I CC'TnA_GC
Cuts at: 0 312 756
Size: 312 444
BsaHI Gr'CG_yC
Cuts at: 0 65 212 223 290 350 654 756
Size: 65 147 11 67 60 304 102
Fragments arranged by size:
304 147 102 67 65 60 11
BsaWI w'CCGG_w
Cuts at: 0 533 756
Size: 533 223
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 294 297 300 756
Size: 294 3 3 456
Fragments arranged by size:
456 294 3 3
BseYI C'CCAG_C
Cuts at: 0 679 721 733 738 756
Size: 679 42 12 5 18
Fragments arranged by size:
679 42 18 12 5
BsiEI CG_ry'CG
Cuts at: 0 11 756
Size: 11 745
BsiHKAI G_wGCw'C
Cuts at: 0 47 77 577 756
Size: 47 30 500 179
Fragments arranged by size:
500 179 47 30
Bsp1286I G_dGCh'C
Cuts at: 0 47 77 109 208 577 666 715 756
Size: 47 30 32 99 369 89 49 41
Fragments arranged by size:
369 99 89 49 47 41 32 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 266 449 653 756
Size: 239 27 183 204 103
Fragments arranged by size:
239 204 183 103 27
BsrBI CCG'CTC
Cuts at: 0 509 756
Size: 509 247
BsrFI r'CCGG_y
Cuts at: 0 115 756
Size: 115 641
BssHII G'CGCG_C
Cuts at: 0 129 136 756
Size: 129 7 620
Fragments arranged by size:
620 129 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 756
Size: 249 507
BtgI C'CryG_G
Cuts at: 0 11 68 78 218 756
Size: 11 57 10 140 538
Fragments arranged by size:
538 140 57 11 10
BtsI GCAGTG_nn'
Cuts at: 0 600 756
Size: 600 156
EaeI y'GGCC_r
Cuts at: 0 8 50 236 251 605 704 756
Size: 8 42 186 15 354 99 52
Fragments arranged by size:
354 186 99 52 42 15 8
EagI C'GGCC_G
Cuts at: 0 8 756
Size: 8 748
EarI CTCTTCn'nnn_
Cuts at: 0 63 756
Size: 63 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 281 351 756
Size: 281 70 405
Fragments arranged by size:
405 281 70
EcoO109I rG'GnC_Cy
Cuts at: 0 106 161 205 756
Size: 106 55 44 551
Fragments arranged by size:
551 106 55 44
HaeII r_GCGC'y
Cuts at: 0 68 215 226 293 353 558 585 657
Size: 68 147 11 67 60 205 27 72
Cuts at: 657 756
Size: 99
Fragments arranged by size:
205 147 99 72 68 67 60 27
11
KasI G'GCGC_C
Cuts at: 0 64 211 222 289 349 653 756
Size: 64 147 11 67 60 304 103
Fragments arranged by size:
304 147 103 67 64 60 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 756
Size: 536 220
MscI TGG'CCA
Cuts at: 0 52 607 756
Size: 52 555 149
Fragments arranged by size:
555 149 52
MspA1I CmG'CkG
Cuts at: 0 80 220 383 756
Size: 80 140 163 373
Fragments arranged by size:
373 163 140 80
NaeI GCC'GGC
Cuts at: 0 117 756
Size: 117 639
NarI GG'CG_CC
Cuts at: 0 65 212 223 290 350 654 756
Size: 65 147 11 67 60 304 102
Fragments arranged by size:
304 147 102 67 65 60 11
NgoMIV G'CCGG_C
Cuts at: 0 115 756
Size: 115 641
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 756
Size: 234 522
PfoI T'CCnGG_A
Cuts at: 0 392 756
Size: 392 364
PpuMI rG'GwC_Cy
Cuts at: 0 161 756
Size: 161 595
PspOMI G'GGCC_C
Cuts at: 0 105 204 756
Size: 105 99 552
Fragments arranged by size:
552 105 99
PstI C_TGCA'G
Cuts at: 0 700 756
Size: 700 56
SacII CC_GC'GG
Cuts at: 0 81 221 756
Size: 81 140 535
Fragments arranged by size:
535 140 81
SanDI GG'GwC_CC
Cuts at: 0 161 756
Size: 161 595
SfcI C'TryA_G
Cuts at: 0 696 756
Size: 696 60
SfoI GGC'GCC
Cuts at: 0 66 213 224 291 351 655 756
Size: 66 147 11 67 60 304 101
Fragments arranged by size:
304 147 101 67 66 60 11
SmaI CCC'GGG
Cuts at: 0 159 167 203 284 756
Size: 159 8 36 81 472
Fragments arranged by size:
472 159 81 36 8
SrfI GCCC'GGGC
Cuts at: 0 203 756
Size: 203 553
StuI AGG'CCT
Cuts at: 0 752 756
Size: 752 4
XmaI C'CCGG_G
Cuts at: 0 157 165 201 282 756
Size: 157 8 36 81 474
Fragments arranged by size:
474 157 81 36 8
XmnI GAAnn'nnTTC
Cuts at: 0 556 756
Size: 556 200
Enzymes that do cut:
AarI AfeI ApaI ApaLI AscI AvaI BanI BanII
BbeI BbvCI BglI Bme1580I BmgBI BplI Bpu10I BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstXI BtgI BtsI EaeI EagI EarI
EciI EcoO109I HaeII KasI MmeI MscI MspA1I NaeI
NarI NgoMIV PflMI PfoI PpuMI PspOMI PstI SacII
SanDI SfcI SfoI SmaI SrfI StuI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApoI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI ZraI