Restriction map for
pFN21AB9648
(Linear) (Six-base) MAPSORT of: KIBB9648.seq Check: 994 from: 1 to: 1173
KIBB9648.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1057 1173
Size: 1057 116
AlwNI CAG_nnn'CTG
Cuts at: 0 507 1173
Size: 507 666
ApaI G_GGCC'C
Cuts at: 0 73 1173
Size: 73 1100
AvaI C'yCGr_G
Cuts at: 0 66 648 810 1009 1173
Size: 66 582 162 199 164
Fragments arranged by size:
582 199 164 162 66
BanI G'GyrC_C
Cuts at: 0 43 127 568 835 925 999 1155 1173
Size: 43 84 441 267 90 74 156 18
Fragments arranged by size:
441 267 156 90 84 74 43 18
BanII G_rGCy'C
Cuts at: 0 73 422 517 946 965 1173
Size: 73 349 95 429 19 208
Fragments arranged by size:
429 349 208 95 73 19
BbeI G_GCGC'C
Cuts at: 0 47 131 572 839 929 1003 1173
Size: 47 84 441 267 90 74 170
Fragments arranged by size:
441 267 170 90 84 74 47
BclI T'GATC_A
Cuts at: 0 1028 1173
Size: 1028 145
BglI GCCn_nnn'nGGC
Cuts at: 0 521 892 1173
Size: 521 371 281
Fragments arranged by size:
521 371 281
Bme1580I G_kGCm'C
Cuts at: 0 73 1009 1160 1173
Size: 73 936 151 13
Fragments arranged by size:
936 151 73 13
BmgBI CAC'GTC
Cuts at: 0 549 1173
Size: 549 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1173
Size: 529 644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1173
Size: 373 800
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 1173
Size: 13 1160
BsaHI Gr'CG_yC
Cuts at: 0 44 128 569 836 926 1000 1173
Size: 44 84 441 267 90 74 173
Fragments arranged by size:
441 267 173 90 84 74 44
BseYI C'CCAG_C
Cuts at: 0 557 939 964 984 1173
Size: 557 382 25 20 189
Fragments arranged by size:
557 382 189 25 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 313 1087 1173
Size: 13 300 774 86
Fragments arranged by size:
774 300 86 13
BsiEI CG_ry'CG
Cuts at: 0 295 364 431 494 605 845 887 914
Size: 295 69 67 63 111 240 42 27
Cuts at: 914 1085 1173
Size: 171 88
Fragments arranged by size:
295 240 171 111 88 69 67 63
42 27
BsiHKAI G_wGCw'C
Cuts at: 0 344 1173
Size: 344 829
Bsp1286I G_dGCh'C
Cuts at: 0 73 344 422 517 946 965 1009 1160
Size: 73 271 78 95 429 19 44 151
Cuts at: 1160 1173
Size: 13
Fragments arranged by size:
429 271 151 95 78 73 44 19
13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 35 1025 1173
Size: 35 990 148
Fragments arranged by size:
990 148 35
BsrFI r'CCGG_y
Cuts at: 0 359 412 430 1048 1097 1173
Size: 359 53 18 618 49 76
Fragments arranged by size:
618 359 76 53 49 18
BsrGI T'GTAC_A
Cuts at: 0 2 1173
Size: 2 1171
BssHII G'CGCG_C
Cuts at: 0 321 323 662 755 869 935 1173
Size: 321 2 339 93 114 66 238
Fragments arranged by size:
339 321 238 114 93 66 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 735 1110 1173
Size: 735 375 63
Fragments arranged by size:
735 375 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 1173
Size: 468 705
BtgI C'CryG_G
Cuts at: 0 186 461 494 605 839 887 1085 1173
Size: 186 275 33 111 234 48 198 88
Fragments arranged by size:
275 234 198 186 111 88 48 33
EaeI y'GGCC_r
Cuts at: 0 292 361 410 428 491 602 608 842
Size: 292 69 49 18 63 111 6 234
Cuts at: 842 884 911 1082 1173
Size: 42 27 171 91
Fragments arranged by size:
292 234 171 111 91 69 63 49
42 27 18 6
EagI C'GGCC_G
Cuts at: 0 292 361 428 491 602 842 884 911
Size: 292 69 67 63 111 240 42 27
Cuts at: 911 1082 1173
Size: 171 91
Fragments arranged by size:
292 240 171 111 91 69 67 63
42 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 113 1173
Size: 113 1060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1173
Size: 529 644
EcoO109I rG'GnC_Cy
Cuts at: 0 51 70 1014 1173
Size: 51 19 944 159
Fragments arranged by size:
944 159 51 19
HaeII r_GCGC'y
Cuts at: 0 47 131 252 507 572 839 929 1003
Size: 47 84 121 255 65 267 90 74
Cuts at: 1003 1173
Size: 170
Fragments arranged by size:
267 255 170 121 90 84 74 65
47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 388 420 1173
Size: 16 32 340 32 753
Fragments arranged by size:
753 340 32 32 16
HincII GTy'rAC
Cuts at: 0 552 1173
Size: 552 621
KasI G'GCGC_C
Cuts at: 0 43 127 568 835 925 999 1173
Size: 43 84 441 267 90 74 174
Fragments arranged by size:
441 267 174 90 84 74 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 938 1173
Size: 803 135 235
Fragments arranged by size:
803 235 135
MslI CAynn'nnrTG
Cuts at: 0 13 466 1173
Size: 13 453 707
Fragments arranged by size:
707 453 13
MspA1I CmG'CkG
Cuts at: 0 188 195 607 630 841 859 968 1081
Size: 188 7 412 23 211 18 109 113
Cuts at: 1081 1087 1173
Size: 6 86
Fragments arranged by size:
412 211 188 113 109 86 23 18
7 6
NaeI GCC'GGC
Cuts at: 0 414 432 1050 1099 1173
Size: 414 18 618 49 74
Fragments arranged by size:
618 414 74 49 18
NarI GG'CG_CC
Cuts at: 0 44 128 569 836 926 1000 1173
Size: 44 84 441 267 90 74 173
Fragments arranged by size:
441 267 173 90 84 74 44
NcoI C'CATG_G
Cuts at: 0 461 1173
Size: 461 712
NgoMIV G'CCGG_C
Cuts at: 0 412 430 1048 1097 1173
Size: 412 18 618 49 76
Fragments arranged by size:
618 412 76 49 18
NotI GC'GGCC_GC
Cuts at: 0 602 842 911 1082 1173
Size: 602 240 69 171 91
Fragments arranged by size:
602 240 171 91 69
NspI r_CATG'y
Cuts at: 0 1038 1173
Size: 1038 135
PasI CC'CwG_GG
Cuts at: 0 930 1173
Size: 930 243
PflMI CCAn_nnn'nTGG
Cuts at: 0 507 1173
Size: 507 666
PpuMI rG'GwC_Cy
Cuts at: 0 1014 1173
Size: 1014 159
PspOMI G'GGCC_C
Cuts at: 0 69 1173
Size: 69 1104
PstI C_TGCA'G
Cuts at: 0 764 1173
Size: 764 409
PvuII CAG'CTG
Cuts at: 0 630 1173
Size: 630 543
SacII CC_GC'GG
Cuts at: 0 189 608 842 1088 1173
Size: 189 419 234 246 85
Fragments arranged by size:
419 246 234 189 85
ScaI AGT'ACT
Cuts at: 0 403 1173
Size: 403 770
SfcI C'TryA_G
Cuts at: 0 455 760 1173
Size: 455 305 413
Fragments arranged by size:
455 413 305
SfoI GGC'GCC
Cuts at: 0 45 129 570 837 927 1001 1173
Size: 45 84 441 267 90 74 172
Fragments arranged by size:
441 267 172 90 84 74 45
SgrAI Cr'CCGG_yG
Cuts at: 0 1048 1173
Size: 1048 125
SmaI CCC'GGG
Cuts at: 0 650 1011 1173
Size: 650 361 162
Fragments arranged by size:
650 361 162
SmlI C'TyrA_G
Cuts at: 0 388 1173
Size: 388 785
StyI C'CwwG_G
Cuts at: 0 131 461 1173
Size: 131 330 712
Fragments arranged by size:
712 330 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 1173
Size: 37 1136
TatI w'GTAC_w
Cuts at: 0 2 401 764 1037 1173
Size: 2 399 363 273 136
Fragments arranged by size:
399 363 273 136 2
Tth111I GACn'n_nGTC
Cuts at: 0 148 1173
Size: 148 1025
XmaI C'CCGG_G
Cuts at: 0 648 1009 1173
Size: 648 361 164
Fragments arranged by size:
648 361 164
XmnI GAAnn'nnTTC
Cuts at: 0 169 1173
Size: 169 1004
Enzymes that do cut:
AlfI AlwNI ApaI AvaI BanI BanII BbeI BclI
BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssHII
BstAPI BstXI BtgI EaeI EagI EciI Eco57MI EcoO109I
HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PflMI PpuMI
PspOMI PstI PvuII SacII ScaI SfcI SfoI SgrAI
SmaI SmlI StyI TaqII TatI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MscI
NdeI NheI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI ZraI