Restriction map for
pFN21AB9645
(Linear) (Six-base) MAPSORT of: KIBB9645.seq Check: 331 from: 1 to: 3096
KIBB9645.orf
With 182 enzymes: SgfI *
March 22, 2012 15:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 358 1653 3096
Size: 358 1295 1443
Fragments arranged by size:
1443 1295 358
AatII G_ACGT'C
Cuts at: 0 499 3096
Size: 499 2597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 805 895 916 1373 2832 2875 3096
Size: 81 724 90 21 457 1459 43 221
Fragments arranged by size:
1459 724 457 221 90 81 43 21
AfeI AGC'GCT
Cuts at: 0 739 2106 3096
Size: 739 1367 990
Fragments arranged by size:
1367 990 739
AflIII A'CryG_T
Cuts at: 0 1769 3096
Size: 1769 1327
AgeI A'CCGG_T
Cuts at: 0 2505 3096
Size: 2505 591
AhdI GACnn_n'nnGTC
Cuts at: 0 2499 3096
Size: 2499 597
AleI CACnn'nnGTG
Cuts at: 0 637 677 2236 3096
Size: 637 40 1559 860
Fragments arranged by size:
1559 860 637 40
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 485 2221 2267 3096
Size: 485 1736 46 829
Fragments arranged by size:
1736 829 485 46
AlwNI CAG_nnn'CTG
Cuts at: 0 939 2690 3096
Size: 939 1751 406
Fragments arranged by size:
1751 939 406
ApaI G_GGCC'C
Cuts at: 0 1948 2632 2938 3096
Size: 1948 684 306 158
Fragments arranged by size:
1948 684 306 158
ApaLI G'TGCA_C
Cuts at: 0 652 2644 3096
Size: 652 1992 452
Fragments arranged by size:
1992 652 452
ApoI r'AATT_y
Cuts at: 0 1441 2737 3096
Size: 1441 1296 359
Fragments arranged by size:
1441 1296 359
AvaI C'yCGr_G
Cuts at: 0 143 1620 2580 3055 3096
Size: 143 1477 960 475 41
Fragments arranged by size:
1477 960 475 143 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 408 441 3096
Size: 408 33 2655
Fragments arranged by size:
2655 408 33
BamHI G'GATC_C
Cuts at: 0 159 3096
Size: 159 2937
BanI G'GyrC_C
Cuts at: 0 461 589 942 1066 1624 3096
Size: 461 128 353 124 558 1472
Fragments arranged by size:
1472 558 461 353 128 124
BanII G_rGCy'C
Cuts at: 0 325 524 1451 1858 1948 2632 2669 2938
Size: 325 199 927 407 90 684 37 269
Cuts at: 2938 2968 3096
Size: 30 128
Fragments arranged by size:
927 684 407 325 269 199 128 90
37 30
BbeI G_GCGC'C
Cuts at: 0 593 946 1070 3096
Size: 593 353 124 2026
Fragments arranged by size:
2026 593 353 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 573 715 1694 2963 3096
Size: 573 142 979 1269 133
Fragments arranged by size:
1269 979 573 142 133
BbvCI CC'TCA_GC
Cuts at: 0 829 3096
Size: 829 2267
BclI T'GATC_A
Cuts at: 0 842 3096
Size: 842 2254
BglI GCCn_nnn'nGGC
Cuts at: 0 1099 3096
Size: 1099 1997
BlpI GC'TnA_GC
Cuts at: 0 258 2101 3096
Size: 258 1843 995
Fragments arranged by size:
1843 995 258
Bme1580I G_kGCm'C
Cuts at: 0 656 1184 1627 1948 2632 2648 2938 3096
Size: 656 528 443 321 684 16 290 158
Fragments arranged by size:
684 656 528 443 321 290 158 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 46 1970 2565 2672 2950 3096
Size: 46 1924 595 107 278 146
Fragments arranged by size:
1924 595 278 146 107 46
BmtI G_CTAG'C
Cuts at: 0 121 3096
Size: 121 2975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1872 3096
Size: 1872 1224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1290 1549 3096
Size: 390 900 259 1547
Fragments arranged by size:
1547 900 390 259
Bpu10I CC'TnA_GC
Cuts at: 0 518 829 959 3096
Size: 518 311 130 2137
Fragments arranged by size:
2137 518 311 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1504 3096
Size: 1096 408 1592
Fragments arranged by size:
1592 1096 408
BsaI GGTCTCn'nnnn_
Cuts at: 0 1827 2587 3096
Size: 1827 760 509
Fragments arranged by size:
1827 760 509
BsaHI Gr'CG_yC
Cuts at: 0 77 496 590 943 1067 2419 3096
Size: 77 419 94 353 124 1352 677
Fragments arranged by size:
1352 677 419 353 124 94 77
BsaWI w'CCGG_w
Cuts at: 0 602 2076 2505 2993 3096
Size: 602 1474 429 488 103
Fragments arranged by size:
1474 602 488 429 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 220 250 685 715 2950 2980
Size: 31 30 159 30 435 30 2235 30
Cuts at: 2980 3096
Size: 116
Fragments arranged by size:
2235 435 159 116 31 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1432 1492 1798 1843 2497 2733 3096
Size: 1432 60 306 45 654 236 363
Fragments arranged by size:
1432 654 363 306 236 60 45
BseYI C'CCAG_C
Cuts at: 0 388 523 1450 1594 3096
Size: 388 135 927 144 1502
Fragments arranged by size:
1502 927 388 144 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 562 1672 1681 1687 1711 3010 3096
Size: 455 107 1110 9 6 24 1299 86
Fragments arranged by size:
1299 1110 455 107 86 24 9 6
BsiEI CG_ry'CG
Cuts at: 0 1649 2262 3096
Size: 1649 613 834
Fragments arranged by size:
1649 834 613
BsiHKAI G_wGCw'C
Cuts at: 0 325 385 656 1715 1858 2648 3096
Size: 325 60 271 1059 143 790 448
Fragments arranged by size:
1059 790 448 325 271 143 60
BsiWI C'GTAC_G
Cuts at: 0 626 1028 3096
Size: 626 402 2068
Fragments arranged by size:
2068 626 402
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7 186 1545 3096
Size: 7 179 1359 1551
Fragments arranged by size:
1551 1359 179 7
Bsp1286I G_dGCh'C
Cuts at: 0 325 385 524 656 1184 1451 1627 1715
Size: 325 60 139 132 528 267 176 88
Cuts at: 1715 1858 1948 2632 2648 2669 2938 2968 3096
Size: 143 90 684 16 21 269 30 128
Fragments arranged by size:
684 528 325 269 267 176 143 139
132 128 90 88 60 30 21 16
BspHI T'CATG_A
Cuts at: 0 2120 3096
Size: 2120 976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 358 1478 1653 3096
Size: 358 1120 175 1443
Fragments arranged by size:
1443 1120 358 175
BsrDI GCAATG_nn'
Cuts at: 0 12 3096
Size: 12 3084
BsrFI r'CCGG_y
Cuts at: 0 79 1299 1641 2217 2505 2525 3096
Size: 79 1220 342 576 288 20 571
Fragments arranged by size:
1220 576 571 342 288 79 20
BsrGI T'GTAC_A
Cuts at: 0 2186 3096
Size: 2186 910
BssSI C'ACGA_G
Cuts at: 0 378 3096
Size: 378 2718
BstAPI GCAn_nnn'nTGC
Cuts at: 0 398 614 1230 1659 3096
Size: 398 216 616 429 1437
Fragments arranged by size:
1437 616 429 398 216
BstBI TT'CG_AA
Cuts at: 0 2908 3096
Size: 2908 188
BstEII G'GTnAC_C
Cuts at: 0 1173 2031 3096
Size: 1173 858 1065
Fragments arranged by size:
1173 1065 858
BstYI r'GATC_y
Cuts at: 0 159 1106 1256 2859 3096
Size: 159 947 150 1603 237
Fragments arranged by size:
1603 947 237 159 150
Bsu36I CC'TnA_GG
Cuts at: 0 1949 3096
Size: 1949 1147
BtgI C'CryG_G
Cuts at: 0 1334 1544 1778 3042 3096
Size: 1334 210 234 1264 54
Fragments arranged by size:
1334 1264 234 210 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2194 3096
Size: 2194 902
BtsI GCAGTG_nn'
Cuts at: 0 172 624 1636 3096
Size: 172 452 1012 1460
Fragments arranged by size:
1460 1012 452 172
DraI TTT'AAA
Cuts at: 0 272 3096
Size: 272 2824
DrdI GACnn_nn'nnGTC
Cuts at: 0 832 2424 3096
Size: 832 1592 672
Fragments arranged by size:
1592 832 672
EaeI y'GGCC_r
Cuts at: 0 476 974 1547 1646 1862 2696 3096
Size: 476 498 573 99 216 834 400
Fragments arranged by size:
834 573 498 476 400 216 99
EagI C'GGCC_G
Cuts at: 0 1646 3096
Size: 1646 1450
EarI CTCTTCn'nnn_
Cuts at: 0 5 141 143 1244 1846 3096
Size: 5 136 2 1101 602 1250
Fragments arranged by size:
1250 1101 602 136 5 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 315 1695 3096
Size: 315 1380 1401
Fragments arranged by size:
1401 1380 315
EcoICRI GAG'CTC
Cuts at: 0 323 1856 3096
Size: 323 1533 1240
Fragments arranged by size:
1533 1240 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 390 805 895 916 1290 1373 1549
Size: 81 309 415 90 21 374 83 176
Cuts at: 1549 2832 2875 3096
Size: 1283 43 221
Fragments arranged by size:
1283 415 374 309 221 176 90 83
81 43 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1474 1864 2137 3096
Size: 1474 390 273 959
Fragments arranged by size:
1474 959 390 273
EcoO109I rG'GnC_Cy
Cuts at: 0 825 936 1482 1944 1945 3096
Size: 825 111 546 462 1 1151
Fragments arranged by size:
1151 825 546 462 111 1
HaeII r_GCGC'y
Cuts at: 0 593 741 931 946 1070 2108 3096
Size: 593 148 190 15 124 1038 988
Fragments arranged by size:
1038 988 593 190 148 124 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 685 717 2485 2517 2776 2808
Size: 166 32 487 32 1768 32 259 32
Cuts at: 2808 2950 2982 3096
Size: 142 32 114
Fragments arranged by size:
1768 487 259 166 142 114 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1815 3096
Size: 1815 1281
KasI G'GCGC_C
Cuts at: 0 589 942 1066 3096
Size: 589 353 124 2030
Fragments arranged by size:
2030 589 353 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2411 2471 2558 2600 3065 3096
Size: 2411 60 87 42 465 31
Fragments arranged by size:
2411 465 87 60 42 31
MscI TGG'CCA
Cuts at: 0 478 1864 2698 3096
Size: 478 1386 834 398
Fragments arranged by size:
1386 834 478 398
MslI CAynn'nnrTG
Cuts at: 0 637 677 2236 2976 3096
Size: 637 40 1559 740 120
Fragments arranged by size:
1559 740 637 120 40
MspA1I CmG'CkG
Cuts at: 0 398 1336 1925 1986 2100 2175 2322 3096
Size: 398 938 589 61 114 75 147 774
Fragments arranged by size:
938 774 589 398 147 114 75 61
NaeI GCC'GGC
Cuts at: 0 81 1301 1643 2527 3096
Size: 81 1220 342 884 569
Fragments arranged by size:
1220 884 569 342 81
NarI GG'CG_CC
Cuts at: 0 590 943 1067 3096
Size: 590 353 124 2029
Fragments arranged by size:
2029 590 353 124
NcoI C'CATG_G
Cuts at: 0 1778 3096
Size: 1778 1318
NgoMIV G'CCGG_C
Cuts at: 0 79 1299 1641 2525 3096
Size: 79 1220 342 884 571
Fragments arranged by size:
1220 884 571 342 79
NheI G'CTAG_C
Cuts at: 0 117 3096
Size: 117 2979
NspI r_CATG'y
Cuts at: 0 795 2208 3096
Size: 795 1413 888
Fragments arranged by size:
1413 888 795
PasI CC'CwG_GG
Cuts at: 0 1735 3096
Size: 1735 1361
PflMI CCAn_nnn'nTGG
Cuts at: 0 719 1170 2694 3096
Size: 719 451 1524 402
Fragments arranged by size:
1524 719 451 402
PpuMI rG'GwC_Cy
Cuts at: 0 825 936 1482 3096
Size: 825 111 546 1614
Fragments arranged by size:
1614 825 546 111
PspOMI G'GGCC_C
Cuts at: 0 1944 2628 2934 3096
Size: 1944 684 306 162
Fragments arranged by size:
1944 684 306 162
PspXI vC'TCGA_Gb
Cuts at: 0 2580 3096
Size: 2580 516
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2796 2828 3096
Size: 2796 32 268
Fragments arranged by size:
2796 268 32
PstI C_TGCA'G
Cuts at: 0 403 491 619 1151 2567 3096
Size: 403 88 128 532 1416 529
Fragments arranged by size:
1416 532 529 403 128 88
PvuII CAG'CTG
Cuts at: 0 398 3096
Size: 398 2698
SacI G_AGCT'C
Cuts at: 0 325 1858 3096
Size: 325 1533 1238
Fragments arranged by size:
1533 1238 325
SacII CC_GC'GG
Cuts at: 0 1337 3096
Size: 1337 1759
SapI GCTCTTCn'nnn_
Cuts at: 0 141 1846 3096
Size: 141 1705 1250
Fragments arranged by size:
1705 1250 141
SbfI CC_TGCA'GG
Cuts at: 0 2567 3096
Size: 2567 529
SfcI C'TryA_G
Cuts at: 0 399 487 615 1147 2563 3096
Size: 399 88 128 532 1416 533
Fragments arranged by size:
1416 533 532 399 128 88
SfoI GGC'GCC
Cuts at: 0 591 944 1068 3096
Size: 591 353 124 2028
Fragments arranged by size:
2028 591 353 124
SmaI CCC'GGG
Cuts at: 0 1622 3096
Size: 1622 1474
SmlI C'TyrA_G
Cuts at: 0 143 1111 1519 2580 3096
Size: 143 968 408 1061 516
Fragments arranged by size:
1061 968 516 408 143
SphI G_CATG'C
Cuts at: 0 2208 3096
Size: 2208 888
StyI C'CwwG_G
Cuts at: 0 1004 1499 1778 1865 3096
Size: 1004 495 279 87 1231
Fragments arranged by size:
1231 1004 495 279 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1300 2143 3096
Size: 1300 843 953
Fragments arranged by size:
1300 953 843
TatI w'GTAC_w
Cuts at: 0 794 2186 2609 3096
Size: 794 1392 423 487
Fragments arranged by size:
1392 794 487 423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 151 183 2748 2780 3096
Size: 151 32 2565 32 316
Fragments arranged by size:
2565 316 151 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 178 3096
Size: 178 2918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 900 1506 1785 2928 3096
Size: 261 639 606 279 1143 168
Fragments arranged by size:
1143 639 606 279 261 168
XhoI C'TCGA_G
Cuts at: 0 143 2580 3096
Size: 143 2437 516
Fragments arranged by size:
2437 516 143
XmaI C'CCGG_G
Cuts at: 0 1620 3096
Size: 1620 1476
XmnI GAAnn'nnTTC
Cuts at: 0 2808 3096
Size: 2808 288
ZraI GAC'GTC
Cuts at: 0 497 3096
Size: 497 2599
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BclI BglI BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PflMI PpuMI PspOMI PspXI PsrI PstI
PvuII SacI SacII SapI SbfI SfcI SfoI SmaI
SmlI SphI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AloI AscI AseI AsiSI
AvrII BcgI BciVI BfrBI BglII BmgBI BsaAI BsaBI
BsmI BspEI BsrBI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PvuI RsrII SalI SanDI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI