Restriction map for
pFN21AB9638
(Linear) (Six-base) MAPSORT of: KIBB9638.seq Check: 9700 from: 1 to: 1353
KIBB9638.orf
With 182 enzymes: SgfI *
July 3, 2012 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 695 1353
Size: 695 658
AccI GT'mk_AC
Cuts at: 0 1232 1353
Size: 1232 121
AflIII A'CryG_T
Cuts at: 0 857 914 1353
Size: 857 57 439
Fragments arranged by size:
857 439 57
AlwNI CAG_nnn'CTG
Cuts at: 0 21 153 1353
Size: 21 132 1200
Fragments arranged by size:
1200 132 21
ApaI G_GGCC'C
Cuts at: 0 34 415 1353
Size: 34 381 938
Fragments arranged by size:
938 381 34
ApaLI G'TGCA_C
Cuts at: 0 349 1353
Size: 349 1004
AvaI C'yCGr_G
Cuts at: 0 433 466 492 693 1060 1353
Size: 433 33 26 201 367 293
Fragments arranged by size:
433 367 293 201 33 26
BanI G'GyrC_C
Cuts at: 0 52 295 625 1353
Size: 52 243 330 728
Fragments arranged by size:
728 330 243 52
BanII G_rGCy'C
Cuts at: 0 34 415 1353
Size: 34 381 938
Fragments arranged by size:
938 381 34
BbeI G_GCGC'C
Cuts at: 0 629 1353
Size: 629 724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1017 1051 1353
Size: 1017 34 302
Fragments arranged by size:
1017 302 34
BglI GCCn_nnn'nGGC
Cuts at: 0 38 85 468 1353
Size: 38 47 383 885
Fragments arranged by size:
885 383 47 38
BlpI GC'TnA_GC
Cuts at: 0 901 1353
Size: 901 452
Bme1580I G_kGCm'C
Cuts at: 0 34 55 353 415 844 1084 1353
Size: 34 21 298 62 429 240 269
Fragments arranged by size:
429 298 269 240 62 34 21
BmgBI CAC'GTC
Cuts at: 0 825 1031 1353
Size: 825 206 322
Fragments arranged by size:
825 322 206
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 1353
Size: 207 1146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 978 1353
Size: 978 375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 784 970 1153 1353
Size: 619 165 186 183 200
Fragments arranged by size:
619 200 186 183 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1060 1353
Size: 910 150 293
Fragments arranged by size:
910 293 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 949 1353
Size: 949 404
BsaHI Gr'CG_yC
Cuts at: 0 77 626 1353
Size: 77 549 727
Fragments arranged by size:
727 549 77
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 245 967 1353
Size: 245 722 386
Fragments arranged by size:
722 386 245
BseYI C'CCAG_C
Cuts at: 0 171 458 748 804 837 1353
Size: 171 287 290 56 33 516
Fragments arranged by size:
516 290 287 171 56 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 544 556 652 673 1297 1353
Size: 166 378 12 96 21 624 56
Fragments arranged by size:
624 378 166 96 56 21 12
BsiEI CG_ry'CG
Cuts at: 0 107 303 1202 1353
Size: 107 196 899 151
Fragments arranged by size:
899 196 151 107
BsiHKAI G_wGCw'C
Cuts at: 0 353 731 1353
Size: 353 378 622
Fragments arranged by size:
622 378 353
Bsp1286I G_dGCh'C
Cuts at: 0 34 55 353 415 731 844 1084 1353
Size: 34 21 298 62 316 113 240 269
Fragments arranged by size:
316 298 269 240 113 62 34 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 29 695 1353
Size: 29 666 658
Fragments arranged by size:
666 658 29
BsrBI CCG'CTC
Cuts at: 0 465 1353
Size: 465 888
BsrFI r'CCGG_y
Cuts at: 0 253 298 1108 1166 1201 1302 1353
Size: 253 45 810 58 35 101 51
Fragments arranged by size:
810 253 101 58 51 45 35
BssHII G'CGCG_C
Cuts at: 0 340 1055 1353
Size: 340 715 298
Fragments arranged by size:
715 340 298
BssSI C'ACGA_G
Cuts at: 0 163 574 586 1099 1353
Size: 163 411 12 513 254
Fragments arranged by size:
513 411 254 163 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 21 1353
Size: 21 1332
BstXI CCAn_nnnn'nTGG
Cuts at: 0 987 1041 1353
Size: 987 54 312
Fragments arranged by size:
987 312 54
BstZ17I GTA'TAC
Cuts at: 0 1233 1353
Size: 1233 120
BtgI C'CryG_G
Cuts at: 0 25 80 107 194 224 630 1220 1353
Size: 25 55 27 87 30 406 590 133
Fragments arranged by size:
590 406 133 87 55 30 27 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1256 1353
Size: 1256 97
BtsI GCAGTG_nn'
Cuts at: 0 1333 1353
Size: 1333 20
DraIII CAC_nnn'GTG
Cuts at: 0 195 1353
Size: 195 1158
DrdI GACnn_nn'nnGTC
Cuts at: 0 963 1353
Size: 963 390
EaeI y'GGCC_r
Cuts at: 0 2 104 300 416 515 582 1199 1353
Size: 2 102 196 116 99 67 617 154
Fragments arranged by size:
617 196 154 116 102 99 67 2
EagI C'GGCC_G
Cuts at: 0 104 300 1199 1353
Size: 104 196 899 154
Fragments arranged by size:
899 196 154 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 56 341 1353
Size: 56 285 1012
Fragments arranged by size:
1012 285 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 784 970 1153 1353
Size: 619 165 186 183 200
Fragments arranged by size:
619 200 186 183 165
EcoO109I rG'GnC_Cy
Cuts at: 0 30 31 891 1252 1353
Size: 30 1 860 361 101
Fragments arranged by size:
860 361 101 30 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 825 1353
Size: 825 528
HaeII r_GCGC'y
Cuts at: 0 629 838 1312 1353
Size: 629 209 474 41
Fragments arranged by size:
629 474 209 41
KasI G'GCGC_C
Cuts at: 0 625 1353
Size: 625 728
MscI TGG'CCA
Cuts at: 0 4 584 1353
Size: 4 580 769
Fragments arranged by size:
769 580 4
MslI CAynn'nnrTG
Cuts at: 0 578 647 1039 1353
Size: 578 69 392 314
Fragments arranged by size:
578 392 314 69
MspA1I CmG'CkG
Cuts at: 0 27 82 109 443 597 792 900 933
Size: 27 55 27 334 154 195 108 33
Cuts at: 933 971 1131 1353
Size: 38 160 222
Fragments arranged by size:
334 222 195 160 154 108 55 38
33 27 27
NaeI GCC'GGC
Cuts at: 0 255 1203 1304 1353
Size: 255 948 101 49
Fragments arranged by size:
948 255 101 49
NarI GG'CG_CC
Cuts at: 0 626 1353
Size: 626 727
NcoI C'CATG_G
Cuts at: 0 1220 1353
Size: 1220 133
NgoMIV G'CCGG_C
Cuts at: 0 253 1201 1302 1353
Size: 253 948 101 51
Fragments arranged by size:
948 253 101 51
NotI GC'GGCC_GC
Cuts at: 0 104 1353
Size: 104 1249
NspI r_CATG'y
Cuts at: 0 124 652 918 1353
Size: 124 528 266 435
Fragments arranged by size:
528 435 266 124
PciI A'CATG_T
Cuts at: 0 914 1353
Size: 914 439
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 1353
Size: 195 1158
PspOMI G'GGCC_C
Cuts at: 0 30 411 1353
Size: 30 381 942
Fragments arranged by size:
942 381 30
PstI C_TGCA'G
Cuts at: 0 899 1130 1353
Size: 899 231 223
Fragments arranged by size:
899 231 223
PvuII CAG'CTG
Cuts at: 0 597 900 1131 1353
Size: 597 303 231 222
Fragments arranged by size:
597 303 231 222
SacII CC_GC'GG
Cuts at: 0 28 83 110 1353
Size: 28 55 27 1243
Fragments arranged by size:
1243 55 28 27
SfcI C'TryA_G
Cuts at: 0 895 1126 1353
Size: 895 231 227
Fragments arranged by size:
895 231 227
SfoI GGC'GCC
Cuts at: 0 627 1353
Size: 627 726
SgrAI Cr'CCGG_yG
Cuts at: 0 1302 1353
Size: 1302 51
SmaI CCC'GGG
Cuts at: 0 435 494 695 1353
Size: 435 59 201 658
Fragments arranged by size:
658 435 201 59
SmlI C'TyrA_G
Cuts at: 0 925 1060 1075 1353
Size: 925 135 15 278
Fragments arranged by size:
925 278 135 15
SphI G_CATG'C
Cuts at: 0 124 1353
Size: 124 1229
StyI C'CwwG_G
Cuts at: 0 1220 1353
Size: 1220 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 973 1353
Size: 973 380
XhoI C'TCGA_G
Cuts at: 0 1060 1353
Size: 1060 293
XmaI C'CCGG_G
Cuts at: 0 433 492 693 1353
Size: 433 59 201 660
Fragments arranged by size:
660 433 201 59
Enzymes that do cut:
AarI AccI AflIII AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstXI BstZ17I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EciI Eco57MI EcoO109I FalI HaeII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PflMI PspOMI PstI PvuII SacII SfcI
SfoI SgrAI SmaI SmlI SphI StyI TaqII XhoI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII BstYI
Bsu36I ClaI CspCI DraI EarI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XmnI ZraI