Restriction map for
pFN21AB9605
(Linear) (Six-base) MAPSORT of: KIBB9605.seq Check: 1981 from: 1 to: 1209
KIBB9605.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1187 1209
Size: 1187 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1209
Size: 868 341
AhdI GACnn_n'nnGTC
Cuts at: 0 84 1209
Size: 84 1125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 514 973 1209
Size: 514 459 236
Fragments arranged by size:
514 459 236
AlwNI CAG_nnn'CTG
Cuts at: 0 244 247 1209
Size: 244 3 962
Fragments arranged by size:
962 244 3
ApaI G_GGCC'C
Cuts at: 0 957 1209
Size: 957 252
ApaLI G'TGCA_C
Cuts at: 0 323 1209
Size: 323 886
AvaI C'yCGr_G
Cuts at: 0 673 685 696 871 1209
Size: 673 12 11 175 338
Fragments arranged by size:
673 338 175 12 11
BanI G'GyrC_C
Cuts at: 0 166 892 942 1020 1209
Size: 166 726 50 78 189
Fragments arranged by size:
726 189 166 78 50
BanII G_rGCy'C
Cuts at: 0 124 957 1209
Size: 124 833 252
Fragments arranged by size:
833 252 124
BbeI G_GCGC'C
Cuts at: 0 170 896 946 1209
Size: 170 726 50 263
Fragments arranged by size:
726 263 170 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 615 1209
Size: 615 594
BbvCI CC'TCA_GC
Cuts at: 0 646 1209
Size: 646 563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 215 1209
Size: 181 34 994
Fragments arranged by size:
994 181 34
BglI GCCn_nnn'nGGC
Cuts at: 0 283 736 791 996 1147 1169 1209
Size: 283 453 55 205 151 22 40
Fragments arranged by size:
453 283 205 151 55 40 22
Bme1580I G_kGCm'C
Cuts at: 0 327 957 1209
Size: 327 630 252
Fragments arranged by size:
630 327 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 786 1209
Size: 79 707 423
Fragments arranged by size:
707 423 79
Bpu10I CC'TnA_GC
Cuts at: 0 646 1209
Size: 646 563
BsaHI Gr'CG_yC
Cuts at: 0 167 893 943 1209
Size: 167 726 50 266
Fragments arranged by size:
726 266 167 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 703 787 922 1209
Size: 238 465 84 135 287
Fragments arranged by size:
465 287 238 135 84
BseYI C'CCAG_C
Cuts at: 0 999 1209
Size: 999 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1209
Size: 120 1089
BsiEI CG_ry'CG
Cuts at: 0 155 1031 1100 1209
Size: 155 876 69 109
Fragments arranged by size:
876 155 109 69
BsiHKAI G_wGCw'C
Cuts at: 0 327 1209
Size: 327 882
BsiWI C'GTAC_G
Cuts at: 0 974 1209
Size: 974 235
BsmBI CGTCTCn'nnnn_
Cuts at: 0 101 1189 1209
Size: 101 1088 20
Fragments arranged by size:
1088 101 20
Bsp1286I G_dGCh'C
Cuts at: 0 124 327 957 1209
Size: 124 203 630 252
Fragments arranged by size:
630 252 203 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1187 1209
Size: 1187 22
BsrBI CCG'CTC
Cuts at: 0 314 966 1041 1209
Size: 314 652 75 168
Fragments arranged by size:
652 314 168 75
BsrFI r'CCGG_y
Cuts at: 0 660 990 1099 1209
Size: 660 330 109 110
Fragments arranged by size:
660 330 110 109
BssHII G'CGCG_C
Cuts at: 0 656 777 1004 1006 1106 1209
Size: 656 121 227 2 100 103
Fragments arranged by size:
656 227 121 103 100 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 244 247 1209
Size: 244 3 962
Fragments arranged by size:
962 244 3
BstYI r'GATC_y
Cuts at: 0 174 1209
Size: 174 1035
Bsu36I CC'TnA_GG
Cuts at: 0 856 1209
Size: 856 353
BtgI C'CryG_G
Cuts at: 0 33 155 386 468 788 938 1209
Size: 33 122 231 82 320 150 271
Fragments arranged by size:
320 271 231 150 122 82 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 366 1209
Size: 357 9 843
Fragments arranged by size:
843 357 9
BtsI GCAGTG_nn'
Cuts at: 0 57 1209
Size: 57 1152
DrdI GACnn_nn'nnGTC
Cuts at: 0 529 1209
Size: 529 680
EaeI y'GGCC_r
Cuts at: 0 140 152 389 1097 1209
Size: 140 12 237 708 112
Fragments arranged by size:
708 237 140 112 12
EagI C'GGCC_G
Cuts at: 0 152 1097 1209
Size: 152 945 112
Fragments arranged by size:
945 152 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 167 277 442 616 1150 1209
Size: 167 110 165 174 534 59
Fragments arranged by size:
534 174 167 165 110 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 786 868 1209
Size: 79 707 82 341
Fragments arranged by size:
707 341 82 79
EcoO109I rG'GnC_Cy
Cuts at: 0 693 953 954 1209
Size: 693 260 1 255
Fragments arranged by size:
693 260 255 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 1209
Size: 477 732
FspI TGC'GCA
Cuts at: 0 832 1209
Size: 832 377
HaeII r_GCGC'y
Cuts at: 0 170 417 645 896 946 1209
Size: 170 247 228 251 50 263
Fragments arranged by size:
263 251 247 228 170 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 1209
Size: 70 32 1107
Fragments arranged by size:
1107 70 32
KasI G'GCGC_C
Cuts at: 0 166 892 942 1209
Size: 166 726 50 267
Fragments arranged by size:
726 267 166 50
MscI TGG'CCA
Cuts at: 0 391 1209
Size: 391 818
MslI CAynn'nnrTG
Cuts at: 0 385 1209
Size: 385 824
MspA1I CmG'CkG
Cuts at: 0 93 157 244 253 470 790 912 940
Size: 93 64 87 9 217 320 122 28
Cuts at: 940 1209
Size: 269
Fragments arranged by size:
320 269 217 122 93 87 64 28
9
NaeI GCC'GGC
Cuts at: 0 992 1101 1209
Size: 992 109 108
Fragments arranged by size:
992 109 108
NarI GG'CG_CC
Cuts at: 0 167 893 943 1209
Size: 167 726 50 266
Fragments arranged by size:
726 266 167 50
NcoI C'CATG_G
Cuts at: 0 386 1209
Size: 386 823
NgoMIV G'CCGG_C
Cuts at: 0 990 1099 1209
Size: 990 109 110
Fragments arranged by size:
990 110 109
NspI r_CATG'y
Cuts at: 0 753 1209
Size: 753 456
PasI CC'CwG_GG
Cuts at: 0 117 560 1209
Size: 117 443 649
Fragments arranged by size:
649 443 117
PspOMI G'GGCC_C
Cuts at: 0 953 1209
Size: 953 256
PstI C_TGCA'G
Cuts at: 0 252 952 1148 1209
Size: 252 700 196 61
Fragments arranged by size:
700 252 196 61
PvuII CAG'CTG
Cuts at: 0 244 1209
Size: 244 965
SacII CC_GC'GG
Cuts at: 0 158 471 791 941 1209
Size: 158 313 320 150 268
Fragments arranged by size:
320 313 268 158 150
SbfI CC_TGCA'GG
Cuts at: 0 952 1148 1209
Size: 952 196 61
Fragments arranged by size:
952 196 61
SfcI C'TryA_G
Cuts at: 0 248 948 1144 1209
Size: 248 700 196 65
Fragments arranged by size:
700 248 196 65
SfoI GGC'GCC
Cuts at: 0 168 894 944 1209
Size: 168 726 50 265
Fragments arranged by size:
726 265 168 50
SmaI CCC'GGG
Cuts at: 0 675 698 873 1209
Size: 675 23 175 336
Fragments arranged by size:
675 336 175 23
SphI G_CATG'C
Cuts at: 0 753 1209
Size: 753 456
SrfI GCCC'GGGC
Cuts at: 0 675 1209
Size: 675 534
StyI C'CwwG_G
Cuts at: 0 386 1209
Size: 386 823
TatI w'GTAC_w
Cuts at: 0 602 1115 1209
Size: 602 513 94
Fragments arranged by size:
602 513 94
Tth111I GACn'n_nGTC
Cuts at: 0 619 1209
Size: 619 590
XmaI C'CCGG_G
Cuts at: 0 673 696 871 1209
Size: 673 23 175 338
Fragments arranged by size:
673 338 175 23
Enzymes that do cut:
AarI AcuI AhdI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I
BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EciI Eco57MI EcoO109I FalI FspI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PspOMI PstI PvuII SacII SbfI SfcI SfoI
SmaI SphI SrfI StyI TatI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI DraIII EarI EcoICRI
EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SspI StuI SwaI TaqII TstI
XbaI XcmI XhoI XmnI ZraI