Restriction map for
pFN21AB9602
(Linear) (Six-base) MAPSORT of: KIBB9602.seq Check: 4155 from: 1 to: 1467
KIBB9602.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AflIII A'CryG_T
Cuts at: 0 788 1467
Size: 788 679
AlwNI CAG_nnn'CTG
Cuts at: 0 303 306 1467
Size: 303 3 1161
Fragments arranged by size:
1161 303 3
ApaI G_GGCC'C
Cuts at: 0 295 1467
Size: 295 1172
AvaI C'yCGr_G
Cuts at: 0 73 592 744 1467
Size: 73 519 152 723
Fragments arranged by size:
723 519 152 73
BanI G'GyrC_C
Cuts at: 0 69 240 265 411 442 448 723 802
Size: 69 171 25 146 31 6 275 79
Cuts at: 802 821 985 1161 1179 1467
Size: 19 164 176 18 288
Fragments arranged by size:
288 275 176 171 164 146 79 69
31 25 19 18 6
BanII G_rGCy'C
Cuts at: 0 295 592 1467
Size: 295 297 875
Fragments arranged by size:
875 297 295
BbeI G_GCGC'C
Cuts at: 0 269 415 446 452 825 1467
Size: 269 146 31 6 373 642
Fragments arranged by size:
642 373 269 146 31 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1436 1467
Size: 1436 31
BglI GCCn_nnn'nGGC
Cuts at: 0 420 850 1467
Size: 420 430 617
Fragments arranged by size:
617 430 420
Bme1580I G_kGCm'C
Cuts at: 0 74 295 1166 1184 1467
Size: 74 221 871 18 283
Fragments arranged by size:
871 283 221 74 18
BmgBI CAC'GTC
Cuts at: 0 789 992 1260 1295 1328 1467
Size: 789 203 268 35 33 139
Fragments arranged by size:
789 268 203 139 35 33
BmtI G_CTAG'C
Cuts at: 0 1153 1467
Size: 1153 314
Bpu10I CC'TnA_GC
Cuts at: 0 1084 1467
Size: 1084 383
BsaBI GATnn'nnATC
Cuts at: 0 577 1467
Size: 577 890
BsaHI Gr'CG_yC
Cuts at: 0 266 412 443 449 822 1467
Size: 266 146 31 6 373 645
Fragments arranged by size:
645 373 266 146 31 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1045 1051 1075 1081 1102 1132 1198 1228
Size: 1045 6 24 6 21 30 66 30
Cuts at: 1228 1467
Size: 239
Fragments arranged by size:
1045 239 66 30 30 24 21 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 1311 1467
Size: 475 836 156
Fragments arranged by size:
836 475 156
BseYI C'CCAG_C
Cuts at: 0 134 296 369 1003 1047 1467
Size: 134 162 73 634 44 420
Fragments arranged by size:
634 420 162 134 73 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1467
Size: 931 536
BsiEI CG_ry'CG
Cuts at: 0 275 338 1142 1467
Size: 275 63 804 325
Fragments arranged by size:
804 325 275 63
BsiWI C'GTAC_G
Cuts at: 0 1118 1467
Size: 1118 349
Bsp1286I G_dGCh'C
Cuts at: 0 74 295 592 1166 1184 1467
Size: 74 221 297 574 18 283
Fragments arranged by size:
574 297 283 221 74 18
BspHI T'CATG_A
Cuts at: 0 569 632 1467
Size: 569 63 835
Fragments arranged by size:
835 569 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1232 1467
Size: 1232 235
BsrBI CCG'CTC
Cuts at: 0 827 1467
Size: 827 640
BsrFI r'CCGG_y
Cuts at: 0 333 445 883 907 1094 1467
Size: 333 112 438 24 187 373
Fragments arranged by size:
438 373 333 187 112 24
BssHII G'CGCG_C
Cuts at: 0 868 1467
Size: 868 599
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 1467
Size: 306 1161
BstYI r'GATC_y
Cuts at: 0 1112 1467
Size: 1112 355
BtgI C'CryG_G
Cuts at: 0 108 806 1467
Size: 108 698 661
Fragments arranged by size:
698 661 108
DrdI GACnn_nn'nnGTC
Cuts at: 0 1099 1398 1467
Size: 1099 299 69
Fragments arranged by size:
1099 299 69
EaeI y'GGCC_r
Cuts at: 0 111 272 335 575 1139 1467
Size: 111 161 63 240 564 328
Fragments arranged by size:
564 328 240 161 111 63
EagI C'GGCC_G
Cuts at: 0 272 335 1139 1467
Size: 272 63 804 328
Fragments arranged by size:
804 328 272 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 1467
Size: 676 791
EcoO109I rG'GnC_Cy
Cuts at: 0 291 292 1193 1338 1467
Size: 291 1 901 145 129
Fragments arranged by size:
901 291 145 129 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 870 1391 1467
Size: 870 521 76
Fragments arranged by size:
870 521 76
HaeII r_GCGC'y
Cuts at: 0 269 415 446 452 825 1307 1467
Size: 269 146 31 6 373 482 160
Fragments arranged by size:
482 373 269 160 146 31 6
HincII GTy'rAC
Cuts at: 0 1034 1104 1221 1467
Size: 1034 70 117 246
Fragments arranged by size:
1034 246 117 70
KasI G'GCGC_C
Cuts at: 0 265 411 442 448 821 1467
Size: 265 146 31 6 373 646
Fragments arranged by size:
646 373 265 146 31 6
MscI TGG'CCA
Cuts at: 0 577 1467
Size: 577 890
MslI CAynn'nnrTG
Cuts at: 0 571 895 1297 1467
Size: 571 324 402 170
Fragments arranged by size:
571 402 324 170
MspA1I CmG'CkG
Cuts at: 0 303 1467
Size: 303 1164
NaeI GCC'GGC
Cuts at: 0 447 909 1467
Size: 447 462 558
Fragments arranged by size:
558 462 447
NarI GG'CG_CC
Cuts at: 0 266 412 443 449 822 1467
Size: 266 146 31 6 373 645
Fragments arranged by size:
645 373 266 146 31 6
NgoMIV G'CCGG_C
Cuts at: 0 445 907 1467
Size: 445 462 560
Fragments arranged by size:
560 462 445
NheI G'CTAG_C
Cuts at: 0 1149 1467
Size: 1149 318
PpuMI rG'GwC_Cy
Cuts at: 0 1193 1467
Size: 1193 274
PspOMI G'GGCC_C
Cuts at: 0 291 1467
Size: 291 1176
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 517 549 631 663 1467
Size: 517 32 82 32 804
Fragments arranged by size:
804 517 82 32 32
PvuII CAG'CTG
Cuts at: 0 303 1467
Size: 303 1164
SfcI C'TryA_G
Cuts at: 0 1020 1467
Size: 1020 447
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 850 1467
Size: 850 617
SfoI GGC'GCC
Cuts at: 0 267 413 444 450 823 1467
Size: 267 146 31 6 373 644
Fragments arranged by size:
644 373 267 146 31 6
SgrAI Cr'CCGG_yG
Cuts at: 0 445 1467
Size: 445 1022
SmaI CCC'GGG
Cuts at: 0 746 1467
Size: 746 721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 682 714 1051 1083 1467
Size: 682 32 337 32 384
Fragments arranged by size:
682 384 337 32 32
XmaI C'CCGG_G
Cuts at: 0 744 1467
Size: 744 723
XmnI GAAnn'nnTTC
Cuts at: 0 640 1467
Size: 640 827
Enzymes that do cut:
AflIII AlwNI ApaI AvaI BanI BanII BbeI BbsI
BglI Bme1580I BmgBI BmtI Bpu10I BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiWI Bsp1286I BspHI BspMI
BsrBI BsrFI BssHII BstAPI BstYI BtgI DrdI EaeI
EagI EciI EcoO109I FalI HaeII HincII KasI MscI
MslI MspA1I NaeI NarI NgoMIV NheI PpuMI PspOMI
PsrI PvuII SfcI SfiI SfoI SgrAI SmaI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaWI BsiHKAI BsmI BsmBI BspEI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EarI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI ZraI