Restriction map for
pFN21AB9596
(Linear) (Six-base) MAPSORT of: KIBB9596.seq Check: 5883 from: 1 to: 1503
KIBB9596.orf
With 182 enzymes: SgfI *
March 22, 2012 15:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 1503
Size: 792 711
AfeI AGC'GCT
Cuts at: 0 1110 1503
Size: 1110 393
AflIII A'CryG_T
Cuts at: 0 461 515 1040 1313 1503
Size: 461 54 525 273 190
Fragments arranged by size:
525 461 273 190 54
AleI CACnn'nnGTG
Cuts at: 0 679 1503
Size: 679 824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 1503
Size: 499 1004
AlwNI CAG_nnn'CTG
Cuts at: 0 434 960 1503
Size: 434 526 543
Fragments arranged by size:
543 526 434
ApaI G_GGCC'C
Cuts at: 0 543 1503
Size: 543 960
AvaI C'yCGr_G
Cuts at: 0 265 697 864 1083 1240 1396 1503
Size: 265 432 167 219 157 156 107
Fragments arranged by size:
432 265 219 167 157 156 107
BanI G'GyrC_C
Cuts at: 0 45 195 396 488 577 786 891 1194
Size: 45 150 201 92 89 209 105 303
Cuts at: 1194 1503
Size: 309
Fragments arranged by size:
309 303 209 201 150 105 92 89
45
BanII G_rGCy'C
Cuts at: 0 543 697 865 1503
Size: 543 154 168 638
Fragments arranged by size:
638 543 168 154
BbeI G_GCGC'C
Cuts at: 0 199 492 581 790 1198 1503
Size: 199 293 89 209 408 305
Fragments arranged by size:
408 305 293 209 199 89
BclI T'GATC_A
Cuts at: 0 635 1503
Size: 635 868
BglI GCCn_nnn'nGGC
Cuts at: 0 113 795 1136 1252 1503
Size: 113 682 341 116 251
Fragments arranged by size:
682 341 251 116 113
BlpI GC'TnA_GC
Cuts at: 0 691 859 1503
Size: 691 168 644
Fragments arranged by size:
691 644 168
Bme1580I G_kGCm'C
Cuts at: 0 50 401 543 1503
Size: 50 351 142 960
Fragments arranged by size:
960 351 142 50
BmgBI CAC'GTC
Cuts at: 0 516 1097 1503
Size: 516 581 406
Fragments arranged by size:
581 516 406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1089 1503
Size: 1089 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1503
Size: 982 521
Bpu10I CC'TnA_GC
Cuts at: 0 55 953 1105 1503
Size: 55 898 152 398
Fragments arranged by size:
898 398 152 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1503
Size: 532 971
BsaHI Gr'CG_yC
Cuts at: 0 116 196 489 568 578 605 652 787
Size: 116 80 293 79 10 27 47 135
Cuts at: 787 1195 1231 1503
Size: 408 36 272
Fragments arranged by size:
408 293 272 135 116 80 79 47
36 27 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1503
Size: 436 30 1037
Fragments arranged by size:
1037 436 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 547 1503
Size: 547 956
BseYI C'CCAG_C
Cuts at: 0 1217 1223 1325 1374 1503
Size: 1217 6 102 49 129
Fragments arranged by size:
1217 129 102 49 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 1476 1503
Size: 1011 465 27
Fragments arranged by size:
1011 465 27
BsiEI CG_ry'CG
Cuts at: 0 1033 1503
Size: 1033 470
BsiHKAI G_wGCw'C
Cuts at: 0 776 1503
Size: 776 727
BsmBI CGTCTCn'nnnn_
Cuts at: 0 679 1503
Size: 679 824
Bsp1286I G_dGCh'C
Cuts at: 0 50 401 543 697 776 865 1503
Size: 50 351 142 154 79 89 638
Fragments arranged by size:
638 351 154 142 89 79 50
BspHI T'CATG_A
Cuts at: 0 824 1503
Size: 824 679
BsrBI CCG'CTC
Cuts at: 0 161 537 1029 1088 1503
Size: 161 376 492 59 415
Fragments arranged by size:
492 415 376 161 59
BsrDI GCAATG_nn'
Cuts at: 0 1425 1503
Size: 1425 78
BsrFI r'CCGG_y
Cuts at: 0 491 666 745 1032 1126 1209 1370 1503
Size: 491 175 79 287 94 83 161 133
Fragments arranged by size:
491 287 175 161 133 94 83 79
BsrGI T'GTAC_A
Cuts at: 0 1013 1503
Size: 1013 490
BssHII G'CGCG_C
Cuts at: 0 7 704 1139 1503
Size: 7 697 435 364
Fragments arranged by size:
697 435 364 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 111 1414 1503
Size: 36 75 1303 89
Fragments arranged by size:
1303 89 75 36
BstYI r'GATC_y
Cuts at: 0 1444 1503
Size: 1444 59
Bsu36I CC'TnA_GG
Cuts at: 0 200 1503
Size: 200 1303
BtgI C'CryG_G
Cuts at: 0 110 191 245 782 1151 1173 1250 1503
Size: 110 81 54 537 369 22 77 253
Fragments arranged by size:
537 369 253 110 81 77 54 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 216 1018 1503
Size: 216 802 485
Fragments arranged by size:
802 485 216
EaeI y'GGCC_r
Cuts at: 0 101 126 248 584 939 1030 1128 1176
Size: 101 25 122 336 355 91 98 48
Cuts at: 1176 1308 1503
Size: 132 195
Fragments arranged by size:
355 336 195 132 122 101 98 91
48 25
EagI C'GGCC_G
Cuts at: 0 1030 1503
Size: 1030 473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 866 1503
Size: 349 517 637
Fragments arranged by size:
637 517 349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 982 1503
Size: 792 190 521
Fragments arranged by size:
792 521 190
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1063 1503
Size: 1063 440
EcoO109I rG'GnC_Cy
Cuts at: 0 204 957 1068 1290 1503
Size: 204 753 111 222 213
Fragments arranged by size:
753 222 213 204 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 297 1503
Size: 297 1206
HaeII r_GCGC'y
Cuts at: 0 199 492 501 581 664 790 907 1112
Size: 199 293 9 80 83 126 117 205
Cuts at: 1112 1198 1503
Size: 86 305
Fragments arranged by size:
305 293 205 199 126 117 86 83
80 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 637 669 1503
Size: 436 32 169 32 834
Fragments arranged by size:
834 436 169 32 32
KasI G'GCGC_C
Cuts at: 0 195 488 577 786 1194 1503
Size: 195 293 89 209 408 309
Fragments arranged by size:
408 309 293 209 195 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1503
Size: 47 1456
MscI TGG'CCA
Cuts at: 0 103 250 1503
Size: 103 147 1253
Fragments arranged by size:
1253 147 103
MslI CAynn'nnrTG
Cuts at: 0 244 679 1503
Size: 244 435 824
Fragments arranged by size:
824 435 244
MspA1I CmG'CkG
Cuts at: 0 193 784 900 1252 1286 1427 1434 1503
Size: 193 591 116 352 34 141 7 69
Fragments arranged by size:
591 352 193 141 116 69 34 7
NaeI GCC'GGC
Cuts at: 0 493 747 1034 1128 1211 1503
Size: 493 254 287 94 83 292
Fragments arranged by size:
493 292 287 254 94 83
NarI GG'CG_CC
Cuts at: 0 196 489 578 787 1195 1503
Size: 196 293 89 209 408 308
Fragments arranged by size:
408 308 293 209 196 89
NcoI C'CATG_G
Cuts at: 0 110 245 1503
Size: 110 135 1258
Fragments arranged by size:
1258 135 110
NgoMIV G'CCGG_C
Cuts at: 0 491 745 1032 1126 1209 1503
Size: 491 254 287 94 83 294
Fragments arranged by size:
491 294 287 254 94 83
NspI r_CATG'y
Cuts at: 0 465 999 1044 1503
Size: 465 534 45 459
Fragments arranged by size:
534 465 459 45
PasI CC'CwG_GG
Cuts at: 0 36 1503
Size: 36 1467
PciI A'CATG_T
Cuts at: 0 461 1040 1503
Size: 461 579 463
Fragments arranged by size:
579 463 461
PflMI CCAn_nnn'nTGG
Cuts at: 0 1374 1503
Size: 1374 129
PpuMI rG'GwC_Cy
Cuts at: 0 204 1290 1503
Size: 204 1086 213
Fragments arranged by size:
1086 213 204
PspOMI G'GGCC_C
Cuts at: 0 539 1503
Size: 539 964
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 277 309 1503
Size: 277 32 1194
Fragments arranged by size:
1194 277 32
PstI C_TGCA'G
Cuts at: 0 713 1503
Size: 713 790
PvuII CAG'CTG
Cuts at: 0 1427 1434 1503
Size: 1427 7 69
Fragments arranged by size:
1427 69 7
SacII CC_GC'GG
Cuts at: 0 194 785 1253 1503
Size: 194 591 468 250
Fragments arranged by size:
591 468 250 194
SexAI A'CCwGG_T
Cuts at: 0 206 1503
Size: 206 1297
SfcI C'TryA_G
Cuts at: 0 222 709 1440 1503
Size: 222 487 731 63
Fragments arranged by size:
731 487 222 63
SfoI GGC'GCC
Cuts at: 0 197 490 579 788 1196 1503
Size: 197 293 89 209 408 307
Fragments arranged by size:
408 307 293 209 197 89
SgrAI Cr'CCGG_yG
Cuts at: 0 491 1503
Size: 491 1012
SmaI CCC'GGG
Cuts at: 0 866 1242 1503
Size: 866 376 261
Fragments arranged by size:
866 376 261
SmlI C'TyrA_G
Cuts at: 0 547 1503
Size: 547 956
SphI G_CATG'C
Cuts at: 0 999 1503
Size: 999 504
StyI C'CwwG_G
Cuts at: 0 110 245 521 572 611 677 1503
Size: 110 135 276 51 39 66 826
Fragments arranged by size:
826 276 135 110 66 51 39
TatI w'GTAC_w
Cuts at: 0 140 1013 1503
Size: 140 873 490
Fragments arranged by size:
873 490 140
XmaI C'CCGG_G
Cuts at: 0 864 1240 1503
Size: 864 376 263
Fragments arranged by size:
864 376 263
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BclI BglI BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstXI BstYI Bsu36I BtgI BtgZI
EaeI EagI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PpuMI PspOMI
PsrI PstI PvuII SacII SexAI SfcI SfoI SgrAI
SmaI SmlI SphI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BfrBI BglII BmrI
BmtI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI
BspMI BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SfiI SgfI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI ZraI